UniProtKB - P10100 (RLPA_ECOLI)
Protein
Endolytic peptidoglycan transglycosylase RlpA
Gene
rlpA
Organism
Escherichia coli (strain K12)
Status
Functioni
Lytic transglycosylase with a strong preference for naked glycan strands that lack stem peptides.UniRule annotation
GO - Molecular functioni
- lyase activity Source: UniProtKB-KW
- lytic endotransglycosylase activity Source: UniProtKB-UniRule
- peptidoglycan binding Source: InterPro
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan metabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10854-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Endolytic peptidoglycan transglycosylase RlpAUniRule annotation (EC:4.2.2.-UniRule annotation)Alternative name(s): Rare lipoprotein A1 Publication |
Gene namesi | Name:rlpA1 PublicationUniRule annotation Ordered Locus Names:b0633, JW0628 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane UniRule annotation; Lipid-anchor UniRule annotation1 Publication
Note: Localizes at the septal ring. Is also associated with some other structures/complexes along the cell cylinder.2 Publications
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell outer membrane Source: EcoCyc
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Deletion does not result in any obvious growth or division defects.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | UniRule annotation1 PublicationAdd BLAST | 17 | |
ChainiPRO_0000030796 | 18 – 362 | Endolytic peptidoglycan transglycosylase RlpAUniRule annotationAdd BLAST | 345 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 18 | N-palmitoyl cysteineUniRule annotation1 Publication | 1 | |
Lipidationi | 18 | S-diacylglycerol cysteineUniRule annotation1 Publication | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P10100 |
PaxDbi | P10100 |
PRIDEi | P10100 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260680, 171 interactors 849624, 1 interactor |
IntActi | P10100, 1 interactor |
STRINGi | 511145.b0633 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 285 – 361 | SPORUniRule annotationAdd BLAST | 77 |
Domaini
The SPOR domain binds septal peptidoglycans and is required to target RlpA to the septal ring.1 Publication
Sequence similaritiesi
Belongs to the RlpA family.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0797, Bacteria |
HOGENOMi | CLU_042923_3_0_6 |
InParanoidi | P10100 |
Family and domain databases
Gene3Di | 2.40.40.10, 1 hit 3.30.70.1070, 1 hit |
HAMAPi | MF_02071, RlpA, 1 hit |
InterProi | View protein in InterPro IPR034718, RlpA IPR009009, RlpA-like_DPBB IPR036908, RlpA-like_sf IPR012997, RplA IPR007730, SPOR-like_dom IPR036680, SPOR-like_sf |
Pfami | View protein in Pfam PF03330, DPBB_1, 1 hit PF05036, SPOR, 1 hit |
SUPFAMi | SSF110997, SSF110997, 1 hit SSF50685, SSF50685, 1 hit |
TIGRFAMsi | TIGR00413, rlpA, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit PS51724, SPOR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P10100-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRKQWLGICI AAGMLAACTS DDGQQQTVSV PQPAVCNGPI VEISGADPRF
60 70 80 90 100
EPLNATANQD YQRDGKSYKI VQDPSRFSQA GLAAIYDAEP GSNLTASGEA
110 120 130 140 150
FDPTQLTAAH PTLPIPSYAR ITNLANGRMI VVRINDRGPY GNDRVISLSR
160 170 180 190 200
AAADRLNTSN NTKVRIDPII VAQDGSLSGP GMACTTVAKQ TYALPAPPDL
210 220 230 240 250
SGGAGTSSVS GPQGDILPVS NSTLKSEDPT GAPVTSSGFL GAPTTLAPGV
260 270 280 290 300
LEGSEPTPAP QPVVTAPSTT PATSPAMVTP QAVSQSASGN FMVQVGAVSD
310 320 330 340 350
QARAQQYQQQ LGQKFGVPGR VTQNGAVWRI QLGPFASKAE ASTLQQRLQT
360
EAQLQSFITT AQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18276 Genomic DNA Translation: AAA24552.1 U82598 Genomic DNA Translation: AAB40833.1 U00096 Genomic DNA Translation: AAC73734.1 AP009048 Genomic DNA Translation: BAA35276.1 M22857 Genomic DNA Translation: AAA24572.1 |
PIRi | A28387, LPECRA |
RefSeqi | NP_415166.1, NC_000913.3 WP_001231430.1, NZ_SSZK01000037.1 |
Genome annotation databases
EnsemblBacteriai | AAC73734; AAC73734; b0633 BAA35276; BAA35276; BAA35276 |
GeneIDi | 57728817 945241 |
KEGGi | ecj:JW0628 eco:b0633 |
PATRICi | fig|1411691.4.peg.1635 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18276 Genomic DNA Translation: AAA24552.1 U82598 Genomic DNA Translation: AAB40833.1 U00096 Genomic DNA Translation: AAC73734.1 AP009048 Genomic DNA Translation: BAA35276.1 M22857 Genomic DNA Translation: AAA24572.1 |
PIRi | A28387, LPECRA |
RefSeqi | NP_415166.1, NC_000913.3 WP_001231430.1, NZ_SSZK01000037.1 |
3D structure databases
SMRi | P10100 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260680, 171 interactors 849624, 1 interactor |
IntActi | P10100, 1 interactor |
STRINGi | 511145.b0633 |
Proteomic databases
jPOSTi | P10100 |
PaxDbi | P10100 |
PRIDEi | P10100 |
Genome annotation databases
EnsemblBacteriai | AAC73734; AAC73734; b0633 BAA35276; BAA35276; BAA35276 |
GeneIDi | 57728817 945241 |
KEGGi | ecj:JW0628 eco:b0633 |
PATRICi | fig|1411691.4.peg.1635 |
Organism-specific databases
EchoBASEi | EB0847 |
Phylogenomic databases
eggNOGi | COG0797, Bacteria |
HOGENOMi | CLU_042923_3_0_6 |
InParanoidi | P10100 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10854-MONOMER |
Miscellaneous databases
PROi | PR:P10100 |
Family and domain databases
Gene3Di | 2.40.40.10, 1 hit 3.30.70.1070, 1 hit |
HAMAPi | MF_02071, RlpA, 1 hit |
InterProi | View protein in InterPro IPR034718, RlpA IPR009009, RlpA-like_DPBB IPR036908, RlpA-like_sf IPR012997, RplA IPR007730, SPOR-like_dom IPR036680, SPOR-like_sf |
Pfami | View protein in Pfam PF03330, DPBB_1, 1 hit PF05036, SPOR, 1 hit |
SUPFAMi | SSF110997, SSF110997, 1 hit SSF50685, SSF50685, 1 hit |
TIGRFAMsi | TIGR00413, rlpA, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit PS51724, SPOR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RLPA_ECOLI | |
Accessioni | P10100Primary (citable) accession number: P10100 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families