rlpA

Functionⁱ

Lytic transglycosylase with a strong preference for naked glycan strands that lack stem peptides.UniRule annotation

GO - Molecular functionⁱ

lyase activity Source: UniProtKB-KW
lytic endotransglycosylase activity Source: UniProtKB-UniRule
peptidoglycan binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell wall organization Source: UniProtKB-KW
peptidoglycan metabolic process Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Biological process	Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG10854-MONOMER

BioCycⁱ

EcoCyc:EG10854-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Endolytic peptidoglycan transglycosylase RlpAUniRule annotation (EC:4.2.2.-UniRule annotation) Alternative name(s): Rare lipoprotein A1 Publication
Gene namesⁱ	Name:rlpA1 PublicationUniRule annotation Ordered Locus Names:b0633, JW0628
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10854 rlpA

EcoGeneⁱ

EG10854 rlpA

Subcellular locationⁱ

Cell membrane
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_02071
; Lipid-anchor
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_02071
1 Publication
Manual assertion inferred by curator fromⁱ
- Ref.1
  "Genes encoding two lipoproteins in the leuS-dacA region of the Escherichia coli chromosome."
  Takase I., Ishino F., Wachi M., Kamata H., Doi M., Asoh S., Matsuzawa H., Ohta T., Matsuhashi M.
  J. Bacteriol. 169:5692-5699(1987) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DIACYLGLYCEROL AT CYS-18, PALMITOYLATION AT CYS-18, SUBCELLULAR LOCATION.

Note:

Localizes at the septal ring. Is also associated with some other structures/complexes along the cell cylinder.2 Publications

GO - Cellular componentⁱ

cell outer membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 22534293
plasma membrane Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Deletion does not result in any obvious growth or division defects.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 17	UniRule annotation1 PublicationAdd BLAST		17
ChainⁱPRO_0000030796	18 – 362	Endolytic peptidoglycan transglycosylase RlpAUniRule annotationAdd BLAST		345

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Lipidationⁱ	18	N-palmitoyl cysteineUniRule annotation1 Publication		1
Lipidationⁱ	18	S-diacylglycerol cysteineUniRule annotation1 Publication		1

Keywords - PTMⁱ

Lipoprotein, Palmitate

Proteomic databases

PaxDbⁱ	P10100
PRIDEⁱ	P10100

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4260680, 171 interactors
IntActⁱ	P10100, 1 interactor
STRINGⁱ	316385.ECDH10B_0702

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P10100
SMRⁱ	P10100
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	285 – 361	SPORUniRule annotationAdd BLAST		77

Domainⁱ

The SPOR domain binds septal peptidoglycans and is required to target RlpA to the septal ring.1 Publication

Sequence similaritiesⁱ

Belongs to the RlpA family.UniRule annotation

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG4105K6T Bacteria COG0797 LUCA
HOGENOMⁱ	HOG000117956
InParanoidⁱ	P10100
KEGG Orthology (KO) More...KOⁱ	K03642
OMAⁱ	PFYSDRI

Family and domain databases

Gene3Dⁱ	2.40.40.10, 1 hit 3.30.70.1070, 1 hit
HAMAPⁱ	MF_02071 RlpA, 1 hit
InterProⁱ	View protein in InterPro IPR034718 RlpA IPR009009 RlpA-like_DPBB IPR036908 RlpA-like_sf IPR012997 RplA IPR007730 SPOR-like_dom IPR036680 SPOR-like_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03330 DPBB_1, 1 hit PF05036 SPOR, 1 hit
SUPFAMⁱ	SSF110997 SSF110997, 1 hit SSF50685 SSF50685, 1 hit
TIGRFAMsⁱ	TIGR00413 rlpA, 1 hit
PROSITEⁱ	View protein in PROSITE PS51257 PROKAR_LIPOPROTEIN, 1 hit PS51724 SPOR, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P10100-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRKQWLGICI AAGMLAACTS DDGQQQTVSV PQPAVCNGPI VEISGADPRF 
        60         70         80         90        100
EPLNATANQD YQRDGKSYKI VQDPSRFSQA GLAAIYDAEP GSNLTASGEA 
       110        120        130        140        150
FDPTQLTAAH PTLPIPSYAR ITNLANGRMI VVRINDRGPY GNDRVISLSR 
       160        170        180        190        200
AAADRLNTSN NTKVRIDPII VAQDGSLSGP GMACTTVAKQ TYALPAPPDL 
       210        220        230        240        250
SGGAGTSSVS GPQGDILPVS NSTLKSEDPT GAPVTSSGFL GAPTTLAPGV 
       260        270        280        290        300
LEGSEPTPAP QPVVTAPSTT PATSPAMVTP QAVSQSASGN FMVQVGAVSD 
       310        320        330        340        350
QARAQQYQQQ LGQKFGVPGR VTQNGAVWRI QLGPFASKAE ASTLQQRLQT 
       360 
EAQLQSFITT AQ

Length:362

Mass (Da):37,528

Last modified:July 1, 1989 - v1

Checksum:ⁱE28B57023CF15686

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M18276 Genomic DNA Translation: AAA24552.1 U82598 Genomic DNA Translation: AAB40833.1 U00096 Genomic DNA Translation: AAC73734.1 AP009048 Genomic DNA Translation: BAA35276.1 M22857 Genomic DNA Translation: AAA24572.1
PIRⁱ	A28387 LPECRA
NCBI Reference Sequences More...RefSeqⁱ	NP_415166.1, NC_000913.3 WP_001231430.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73734; AAC73734; b0633 BAA35276; BAA35276; BAA35276
GeneIDⁱ	945241
KEGGⁱ	ecj:JW0628 eco:b0633
PATRICⁱ	fig\|1411691.4.peg.1635

Protein	Similar proteins		Species	Score	Length	Source
P10100	Endolytic peptidoglycan transglycosylase RlpA		ECOLX		362	UniRef100_P10100
Endolytic peptidoglycan transglycosylase RlpA		SHIBO		362
Endolytic peptidoglycan transglycosylase RlpA		Escherichia coli UMNK88		362
Endolytic peptidoglycan transglycosylase RlpA		Escherichia coli 2-210-07_S3_C3		362
Endolytic peptidoglycan transglycosylase RlpA		Escherichia coli DEC6A		362
+14

Protein	Similar proteins		Species	Score	Length	Source
P10100	Endolytic peptidoglycan transglycosylase RlpA		ECO57		362	UniRef90_P10100
Endolytic peptidoglycan transglycosylase RlpA		ECOLX		362
Endolytic peptidoglycan transglycosylase RlpA		SHIBO		362
Endolytic peptidoglycan transglycosylase RlpA		Escherichia coli UMNK88		362
Endolytic peptidoglycan transglycosylase RlpA		Escherichia coli 2-210-07_S3_C3		362
+645

Protein	Similar proteins		Species	Score	Length	Source
P10100	Endolytic peptidoglycan transglycosylase RlpA		ECO57		362	UniRef50_P10100
Endolytic peptidoglycan transglycosylase RlpA		SALTY		381
Endolytic peptidoglycan transglycosylase RlpA		SALTI		377
Endolytic peptidoglycan transglycosylase RlpA		YERPE		360
Endolytic peptidoglycan transglycosylase RlpA		ECOLX		362
+2378

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M18276 Genomic DNA Translation: AAA24552.1 U82598 Genomic DNA Translation: AAB40833.1 U00096 Genomic DNA Translation: AAC73734.1 AP009048 Genomic DNA Translation: BAA35276.1 M22857 Genomic DNA Translation: AAA24572.1
PIRⁱ	A28387 LPECRA
RefSeqⁱ	NP_415166.1, NC_000913.3 WP_001231430.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P10100
SMRⁱ	P10100
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260680, 171 interactors
IntActⁱ	P10100, 1 interactor
STRINGⁱ	316385.ECDH10B_0702

Proteomic databases

PaxDbⁱ	P10100
PRIDEⁱ	P10100

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73734; AAC73734; b0633 BAA35276; BAA35276; BAA35276
GeneIDⁱ	945241
KEGGⁱ	ecj:JW0628 eco:b0633
PATRICⁱ	fig\|1411691.4.peg.1635

Organism-specific databases

EchoBASEⁱ	EB0847
EcoGeneⁱ	EG10854 rlpA

Phylogenomic databases

eggNOGⁱ	ENOG4105K6T Bacteria COG0797 LUCA
HOGENOMⁱ	HOG000117956
InParanoidⁱ	P10100
KOⁱ	K03642
OMAⁱ	PFYSDRI

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG10854-MONOMER

BioCycⁱ

EcoCyc:EG10854-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P10100

PROⁱ

PR:P10100

Family and domain databases

Gene3Dⁱ	2.40.40.10, 1 hit 3.30.70.1070, 1 hit
HAMAPⁱ	MF_02071 RlpA, 1 hit
InterProⁱ	View protein in InterPro IPR034718 RlpA IPR009009 RlpA-like_DPBB IPR036908 RlpA-like_sf IPR012997 RplA IPR007730 SPOR-like_dom IPR036680 SPOR-like_sf
Pfamⁱ	View protein in Pfam PF03330 DPBB_1, 1 hit PF05036 SPOR, 1 hit
SUPFAMⁱ	SSF110997 SSF110997, 1 hit SSF50685 SSF50685, 1 hit
TIGRFAMsⁱ	TIGR00413 rlpA, 1 hit
PROSITEⁱ	View protein in PROSITE PS51257 PROKAR_LIPOPROTEIN, 1 hit PS51724 SPOR, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RLPA_ECOLI
Accessionⁱ	P10100Primary (citable) accession number: P10100
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	July 1, 1989
	Last modified:	October 10, 2018
	This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P10100 (RLPA_ECOLI)

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Endolytic peptidoglycan transglycosylase RlpA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ