UniProtKB - P10088 (TFDA_CUPPJ)
Protein
Alpha-ketoglutarate-dependent 2,4-dichlorophenoxyacetate dioxygenase
Gene
tfdA
Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Cupriavidus necator (strain JMP 134))
Status
Functioni
Involved in degradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2-methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid.
Catalytic activityi
Cofactori
Fe2+Note: Binds 1 Fe2+ ion per subunit.
Activity regulationi
Activated by ascorbate.Curated
: (2,4-dichlorophenoxy)acetate degradation Pathwayi
This protein is involved in the pathway (2,4-dichlorophenoxy)acetate degradation, which is part of Xenobiotic degradation.View all proteins of this organism that are known to be involved in the pathway (2,4-dichlorophenoxy)acetate degradation and in Xenobiotic degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 114 | Iron; catalyticCurated | 1 | |
Metal bindingi | 116 | Iron; catalyticCurated | 1 | |
Binding sitei | 141 | 2-oxoglutarateBy similarity | 1 | |
Binding sitei | 248 | 2-oxoglutarateBy similarity | 1 | |
Metal bindingi | 263 | Iron; catalyticCurated | 1 | |
Binding sitei | 274 | 2-oxoglutarateBy similarity | 1 | |
Binding sitei | 278 | 2-oxoglutarateBy similarity | 1 |
GO - Molecular functioni
- dioxygenase activity Source: UniProtKB-KW
- L-ascorbic acid binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- 2,4-dichlorophenoxyacetic acid catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Aromatic hydrocarbons catabolism |
Ligand | Iron, Metal-binding, Vitamin C |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-14384 |
UniPathwayi | UPA00685 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-ketoglutarate-dependent 2,4-dichlorophenoxyacetate dioxygenase (EC:1.14.11.-)Short name: 2,4-D dioxygenase |
Gene namesi | Name:tfdA Ordered Locus Names:Reut_D6479 |
Encoded oni | Plasmid pJP45 Publications Plasmid pPJ41 Publication |
Organismi | Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Cupriavidus necator (strain JMP 134)) |
Taxonomic identifieri | 264198 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | H → A: No change in activity and substrate affinity. 1 Publication | 1 | |
Mutagenesisi | 114 | H → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 116 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 168 | H → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 201 | H → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 214 | H → A: 10-fold decrease in affinity for 2,4-D and highly reduced activity. 1 Publication | 1 | |
Mutagenesisi | 217 | H → A: 2.5-fold decrease in affinity for 2,4-D but no change in activity. 1 Publication | 1 | |
Mutagenesisi | 236 | H → A: No change in activity and substrate affinity. 1 Publication | 1 | |
Mutagenesisi | 246 | H → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 263 | H → A: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194017 | 1 – 287 | Alpha-ketoglutarate-dependent 2,4-dichlorophenoxyacetate dioxygenaseAdd BLAST | 287 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 113 | 3-hydroxytryptophan; by autocatalysis1 Publication | 1 |
Post-translational modificationi
Hydroxylated on Trp-113; inactivates the enzyme.1 Publication
Keywords - PTMi
HydroxylationStructurei
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the TfdA dioxygenase family.Curated
Phylogenomic databases
HOGENOMi | HOG000165071 |
KOi | K06912 |
OMAi | RDWATQP |
OrthoDBi | 1742732at2 |
Family and domain databases
Gene3Di | 3.60.130.10, 1 hit |
InterProi | View protein in InterPro IPR042098 TauD-like_sf IPR003819 TauD/TfdA-like |
Pfami | View protein in Pfam PF02668 TauD, 1 hit |
i Sequence
Sequence statusi: Complete.
P10088-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVVANPLHP LFAAGVEDID LREALGSTEV REIERLMDEK SVLVFRGQPL
60 70 80 90 100
SQDQQIAFAR NFGPLEGGFI KVNQRPSRFK YAELADISNV SLDGKVAQRD
110 120 130 140 150
AREVVGNFAN QLWHSDSSFQ QPAARYSMLS AVVVPPSGGD TEFCDMRAAY
160 170 180 190 200
DALPRDLQSE LEGLRAEHYA LNSRFLLGDT DYSEAQRNAM PPVNWPLVRT
210 220 230 240 250
HAGSGRKFLF IGAHASHVEG LPVAEGRMLL AELLEHATQR EFVYRHRWNV
260 270 280
GDLVMWDNRC VLHRGRRYDI SARRELRRAT TLDDAVV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16730 Genomic DNA Translation: AAA21983.1 AY365053 Genomic DNA Translation: AAR31052.1 EU827467 Genomic DNA Translation: ACF35494.1 CP000093 Genomic DNA Translation: AAZ65777.1 |
PIRi | A27082 |
RefSeqi | WP_011178399.1, NC_007337.1 |
Genome annotation databases
EnsemblBacteriai | AAZ65777; AAZ65777; Reut_D6479 |
KEGGi | reu:Reut_D6479 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16730 Genomic DNA Translation: AAA21983.1 AY365053 Genomic DNA Translation: AAR31052.1 EU827467 Genomic DNA Translation: ACF35494.1 CP000093 Genomic DNA Translation: AAZ65777.1 |
PIRi | A27082 |
RefSeqi | WP_011178399.1, NC_007337.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GQX | model | - | A | 1-287 | [»] | |
ModBasei | Search... | |||||
SWISS-MODEL-Workspacei | Submit a new modelling project... | |||||
PDBe-KBi | Search... |
Genome annotation databases
EnsemblBacteriai | AAZ65777; AAZ65777; Reut_D6479 |
KEGGi | reu:Reut_D6479 |
Phylogenomic databases
HOGENOMi | HOG000165071 |
KOi | K06912 |
OMAi | RDWATQP |
OrthoDBi | 1742732at2 |
Enzyme and pathway databases
UniPathwayi | UPA00685 |
BioCyci | MetaCyc:MONOMER-14384 |
Family and domain databases
Gene3Di | 3.60.130.10, 1 hit |
InterProi | View protein in InterPro IPR042098 TauD-like_sf IPR003819 TauD/TfdA-like |
Pfami | View protein in Pfam PF02668 TauD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TFDA_CUPPJ | |
Accessioni | P10088Primary (citable) accession number: P10088 Secondary accession number(s): B5A9M3, Q46M53 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | October 16, 2019 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Plasmid, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references