UniProtKB - P10078 (ZFP28_MOUSE)
Protein
Zinc finger protein 28
Gene
Zfp28
Organism
Mus musculus (Mouse)
Status
Functioni
May be involved in transcriptional regulation. May have a role in embryonic development.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 377 – 399 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 405 – 427 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 433 – 456 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 462 – 484 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 490 – 512 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 518 – 540 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 546 – 568 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 574 – 596 | C2H2-type 8PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 602 – 624 | C2H2-type 9PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 630 – 652 | C2H2-type 10PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 658 – 680 | C2H2-type 11PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 686 – 708 | C2H2-type 12PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 714 – 736 | C2H2-type 13PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 742 – 764 | C2H2-type 14PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 770 – 792 | C2H2-type 15; degeneratePROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-212436, Generic Transcription Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein 28Short name: Zfp-28 Alternative name(s): Protein mKR5 |
Gene namesi | Name:Zfp28 Synonyms:Mkr5, Zfp-28 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99175, Zfp28 |
Subcellular locationi
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047290 | 1 – 825 | Zinc finger protein 28Add BLAST | 825 |
Proteomic databases
MaxQBi | P10078 |
PaxDbi | P10078 |
PRIDEi | P10078 |
PTM databases
iPTMneti | P10078 |
PhosphoSitePlusi | P10078 |
Expressioni
Tissue specificityi
Expressed predominantly in ovary.
Developmental stagei
Expression decreases in embryo after day 16.
Gene expression databases
Bgeei | ENSMUSG00000062861, Expressed in head and 214 other tissues |
Genevisiblei | P10078, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 204654, 34 interactors |
STRINGi | 10090.ENSMUSP00000079812 |
Miscellaneous databases
RNActi | P10078, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 103 – 174 | KRABPROSITE-ProRule annotationAdd BLAST | 72 |
Sequence similaritiesi
Belongs to the krueppel C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 377 – 399 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 405 – 427 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 433 – 456 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 462 – 484 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 490 – 512 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 518 – 540 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 546 – 568 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 574 – 596 | C2H2-type 8PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 602 – 624 | C2H2-type 9PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 630 – 652 | C2H2-type 10PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 658 – 680 | C2H2-type 11PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 686 – 708 | C2H2-type 12PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 714 – 736 | C2H2-type 13PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 742 – 764 | C2H2-type 14PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 770 – 792 | C2H2-type 15; degeneratePROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162376 |
HOGENOMi | CLU_002678_44_5_1 |
InParanoidi | P10078 |
OMAi | QETQFRQ |
TreeFami | TF341817 |
Family and domain databases
CDDi | cd07765, KRAB_A-box, 1 hit |
InterProi | View protein in InterPro IPR001909, KRAB IPR036051, KRAB_dom_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF01352, KRAB, 1 hit PF00096, zf-C2H2, 11 hits |
SMARTi | View protein in SMART SM00349, KRAB, 1 hit SM00355, ZnF_C2H2, 14 hits |
SUPFAMi | SSF109640, SSF109640, 1 hit SSF57667, SSF57667, 8 hits |
PROSITEi | View protein in PROSITE PS50805, KRAB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 14 hits PS50157, ZINC_FINGER_C2H2_2, 14 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P10078-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQGMASVVSC EPWALLGRGA LCTKARPGGG PAAGTVVAPG SPDRGRPRSR
60 70 80 90 100
NSLASQDQQG AVTSGTAHKA LFSRDTNFLQ EINRKQEAAP TGTRHKAKSQ
110 120 130 140 150
GLVTFGDVAV VFSQEEWEWL NSEQRSLYWK VMLDNYRNLA SLGLCASQPD
160 170 180 190 200
MITSLEQGRD PWMMKRKMRK GQHLDLKAMQ ETKEFPPKDL SEETLFLAVL
210 220 230 240 250
RKQLLPHRPK CSMVRAAWEG GAVFTTHRGL KTNSGLARDS PAQLVSAQRS
260 270 280 290 300
FCKSVTWENC GDRGSVGQQS VQEAQDLLPR QDSHAERVTG RTWSTKLECS
310 320 330 340 350
TFRDQDSECT FERNEQETVT PNRAFSEGRD GMCIESGRWF HLNSSDERSH
360 370 380 390 400
NCDSGKSFSS NPVVVKETGI CSGKKLFQCN ECKKTFTQSS SLTVHQRIHT
410 420 430 440 450
GEKPYKCNQC GKAFSDGSSF ARHQRCHTGK KPYECPECGK AFIQNTSLVR
460 470 480 490 500
HWRYYHTGEK PFDCIDCGKA FSDHIGLNQH RRIHTGEKPY TCEVCHKSFR
510 520 530 540 550
YGSSLTVHQR IHTGEKPYEC EICRKAFSHH ASLTQHQRVH SGEKPFKCKE
560 570 580 590 600
CGKAFRQNIH LASHWRIHTG EKPFECGECG KSFSISSQLA THQRIHTGEK
610 620 630 640 650
PYECKVCRKA FTQKAHLAQH QKTHTGEKPY ECKECGKAFS QTTHLIQHQR
660 670 680 690 700
VHTGEKPYKC LECGKAFGDN SSCTQHRRLH TGQRPYECVE CGKTFKTKSS
710 720 730 740 750
LICHRRCHTG EKPYECSACG KAFSHRQSLS VHQRIHSGKK PYECKECRKT
760 770 780 790 800
FIQIGHLNQH KRVHTGERTY NYKKGRRAFR QTAHFAHHQQ IHSGKSPAHH
810 820
SLPSTSNPVD LFSKFVWNPS SLPSS
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LIA3 | A0A140LIA3_MOUSE | Zinc finger protein 28 | Zfp28 | 171 | Annotation score: | ||
A0A140LJ19 | A0A140LJ19_MOUSE | Zinc finger protein 28 | Zfp28 | 161 | Annotation score: | ||
A0A140LIZ0 | A0A140LIZ0_MOUSE | Zinc finger protein 28 | Zfp28 | 58 | Annotation score: | ||
A0A140LIS3 | A0A140LIS3_MOUSE | Zinc finger protein 28 | Zfp28 | 30 | Annotation score: | ||
A0A140LJB6 | A0A140LJB6_MOUSE | Zinc finger protein 28 | Zfp28 | 56 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 263 | R → W in BAC33362 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 426 | C → Y in CAA31107 (PubMed:3143103).Curated | 1 | |
Sequence conflicti | 426 | C → Y in AAA37120 (PubMed:3143103).Curated | 1 | |
Sequence conflicti | 677 | R → Q (PubMed:3143103).Curated | 1 | |
Sequence conflicti | 677 | R → Q (PubMed:7958967).Curated | 1 | |
Sequence conflicti | 683 | Q → L (PubMed:3143103).Curated | 1 | |
Sequence conflicti | 683 | Q → L (PubMed:7958967).Curated | 1 | |
Sequence conflicti | 704 – 705 | HR → YC (PubMed:3143103).Curated | 2 | |
Sequence conflicti | 704 – 705 | HR → YC (PubMed:7958967).Curated | 2 | |
Sequence conflicti | 726 | R → W (PubMed:3143103).Curated | 1 | |
Sequence conflicti | 726 | R → W (PubMed:7958967).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 658 | Y → I in strain: 129/Sv; requires 2 nucleotide substitutions. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006882 | 600 | K → KPFECKVCRKAFRQNIHLAS HWRIHTGEKPFECGECGKSF SISSQLATHQRIHTGEK in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028202 mRNA Translation: BAC25810.1 AK048530 mRNA Translation: BAC33362.1 BC062116 mRNA Translation: AAH62116.1 X12594 mRNA Translation: CAA31107.1 M36516 mRNA Translation: AAA37120.1 L28802 Genomic DNA Translation: AAA64428.1 |
CCDSi | CCDS39752.1 [P10078-1] |
PIRi | I53859 |
RefSeqi | NP_780456.2, NM_175247.3 [P10078-1] |
Genome annotation databases
Ensembli | ENSMUST00000081022; ENSMUSP00000079812; ENSMUSG00000062861 [P10078-1] |
GeneIDi | 22690 |
KEGGi | mmu:22690 |
UCSCi | uc009fbi.1, mouse [P10078-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028202 mRNA Translation: BAC25810.1 AK048530 mRNA Translation: BAC33362.1 BC062116 mRNA Translation: AAH62116.1 X12594 mRNA Translation: CAA31107.1 M36516 mRNA Translation: AAA37120.1 L28802 Genomic DNA Translation: AAA64428.1 |
CCDSi | CCDS39752.1 [P10078-1] |
PIRi | I53859 |
RefSeqi | NP_780456.2, NM_175247.3 [P10078-1] |
3D structure databases
SMRi | P10078 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204654, 34 interactors |
STRINGi | 10090.ENSMUSP00000079812 |
PTM databases
iPTMneti | P10078 |
PhosphoSitePlusi | P10078 |
Proteomic databases
MaxQBi | P10078 |
PaxDbi | P10078 |
PRIDEi | P10078 |
Protocols and materials databases
Antibodypediai | 33216, 65 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000081022; ENSMUSP00000079812; ENSMUSG00000062861 [P10078-1] |
GeneIDi | 22690 |
KEGGi | mmu:22690 |
UCSCi | uc009fbi.1, mouse [P10078-1] |
Organism-specific databases
CTDi | 140612 |
MGIi | MGI:99175, Zfp28 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162376 |
HOGENOMi | CLU_002678_44_5_1 |
InParanoidi | P10078 |
OMAi | QETQFRQ |
TreeFami | TF341817 |
Enzyme and pathway databases
Reactomei | R-MMU-212436, Generic Transcription Pathway |
Miscellaneous databases
BioGRID-ORCSi | 22690, 1 hit in 17 CRISPR screens |
ChiTaRSi | Zfp28, mouse |
PROi | PR:P10078 |
RNActi | P10078, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000062861, Expressed in head and 214 other tissues |
Genevisiblei | P10078, MM |
Family and domain databases
CDDi | cd07765, KRAB_A-box, 1 hit |
InterProi | View protein in InterPro IPR001909, KRAB IPR036051, KRAB_dom_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF01352, KRAB, 1 hit PF00096, zf-C2H2, 11 hits |
SMARTi | View protein in SMART SM00349, KRAB, 1 hit SM00355, ZnF_C2H2, 14 hits |
SUPFAMi | SSF109640, SSF109640, 1 hit SSF57667, SSF57667, 8 hits |
PROSITEi | View protein in PROSITE PS50805, KRAB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 14 hits PS50157, ZINC_FINGER_C2H2_2, 14 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZFP28_MOUSE | |
Accessioni | P10078Primary (citable) accession number: P10078 Secondary accession number(s): Q61776, Q8BT11, Q8C836 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | August 4, 2003 | |
Last modified: | December 2, 2020 | |
This is version 192 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families