UniProtKB - P10037 (PIT1_RAT)
Protein
Pituitary-specific positive transcription factor 1
Gene
Pou1f1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 214 – 273 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- chromatin binding Source: Ensembl
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- RNA polymerase II activating transcription factor binding Source: Ensembl
- RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II transcription factor binding Source: Ensembl
GO - Biological processi
- adenohypophysis development Source: RGD
- B cell differentiation Source: Ensembl
- cell fate specification Source: Ensembl
- determination of adult lifespan Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of cell population proliferation Source: Ensembl
- positive regulation of inositol trisphosphate biosynthetic process Source: Ensembl
- positive regulation of multicellular organism growth Source: Ensembl
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of insulin-like growth factor receptor signaling pathway Source: Ensembl
- somatotropin secreting cell development Source: Ensembl
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Pituitary-specific positive transcription factor 1Short name: PIT-1 Alternative name(s): Growth hormone factor 1 Short name: GHF-1 |
Gene namesi | Name:Pou1f1 Synonyms:Ghf-1, Pit-1, Pit1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3367 Pou1f1 |
Subcellular locationi
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100701 | 1 – 291 | Pituitary-specific positive transcription factor 1Add BLAST | 291 |
Proteomic databases
PRIDEi | P10037 |
PTM databases
iPTMneti | P10037 |
PhosphoSitePlusi | P10037 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000000715 Expressed in 4 organ(s), highest expression level in brain |
Genevisiblei | P10037 RN |
Interactioni
Subunit structurei
Interacts with PITX1.
Interacts with LHX3.
Interacts with ELK1.
By similarityBinary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
Q99697-3 | 2 | EBI-9825525,EBI-1175243 | From a different organism. |
GO - Molecular functioni
- RNA polymerase II activating transcription factor binding Source: Ensembl
- RNA polymerase II transcription factor binding Source: Ensembl
Protein-protein interaction databases
BioGridi | 247549, 4 interactors |
CORUMi | P10037 |
IntActi | P10037, 1 interactor |
STRINGi | 10116.ENSRNOP00000000905 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P10037 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P10037 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 124 – 198 | POU-specificPROSITE-ProRule annotationAdd BLAST | 75 |
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
GeneTreei | ENSGT00940000158913 |
InParanoidi | P10037 |
OMAi | FPEHALS |
OrthoDBi | 797288at2759 |
PhylomeDBi | P10037 |
TreeFami | TF316413 |
Family and domain databases
CDDi | cd00086 homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057 Homeobox-like_sf IPR017970 Homeobox_CS IPR001356 Homeobox_dom IPR010982 Lambda_DNA-bd_dom_sf IPR015586 Pit_1 IPR013847 POU IPR000327 POU_dom |
PANTHERi | PTHR11636:SF84 PTHR11636:SF84, 1 hit |
Pfami | View protein in Pfam PF00046 Homeodomain, 1 hit PF00157 Pou, 1 hit |
PRINTSi | PR00028 POUDOMAIN |
SMARTi | View protein in SMART SM00389 HOX, 1 hit SM00352 POU, 1 hit |
SUPFAMi | SSF46689 SSF46689, 1 hit SSF47413 SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027 HOMEOBOX_1, 1 hit PS50071 HOMEOBOX_2, 1 hit PS00035 POU_1, 1 hit PS00465 POU_2, 1 hit PS51179 POU_3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform B (identifier: P10037-1) [UniParc]FASTAAdd to basket
Also known as: PIT-1B, GHF-1
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSCQPFTSAD TFIPLNSDAS AALPLRMHHS AAEGLPASNH ATNVMSTATG
60 70 80 90 100
LHYSVPSCHY GNQPSTYGVM AGTLTPCLYK FPDHTLSHGF PPLHQPLLAE
110 120 130 140 150
DPTASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ
160 170 180 190 200
TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV
210 220 230 240 250
GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEHSKPS SQEIMRMAEE
260 270 280 290
LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R
Sequence cautioni
The sequence AAA41851 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAA41853 differs from that shown. Reason: Erroneous initiation.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002316 | 47 | T → TVPSILSLIQTPKCLHTYFS MTTMGNT in isoform A. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65364 X65368 Genomic DNA Translation: CAA46440.1 L01506 mRNA Translation: AAA41850.1 L01506 mRNA Translation: AAA41851.1 Different initiation. L01507 mRNA Translation: AAA41852.1 L01507 mRNA Translation: AAA41853.1 Different initiation. S97398 mRNA Translation: AAC60656.1 M23253 mRNA Translation: AAA41854.1 X12658 mRNA Translation: CAA31185.1 X63089 mRNA No translation available. |
PIRi | S22705 |
RefSeqi | XP_006248036.1, XM_006247974.1 [P10037-2] |
Genome annotation databases
Ensembli | ENSRNOT00000000905; ENSRNOP00000000905; ENSRNOG00000000715 [P10037-1] |
GeneIDi | 25517 |
UCSCi | RGD:3367 rat [P10037-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65364 X65368 Genomic DNA Translation: CAA46440.1 L01506 mRNA Translation: AAA41850.1 L01506 mRNA Translation: AAA41851.1 Different initiation. L01507 mRNA Translation: AAA41852.1 L01507 mRNA Translation: AAA41853.1 Different initiation. S97398 mRNA Translation: AAC60656.1 M23253 mRNA Translation: AAA41854.1 X12658 mRNA Translation: CAA31185.1 X63089 mRNA No translation available. |
PIRi | S22705 |
RefSeqi | XP_006248036.1, XM_006247974.1 [P10037-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AU7 | X-ray | 2.30 | A/B | 128-273 | [»] | |
SMRi | P10037 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 247549, 4 interactors |
CORUMi | P10037 |
IntActi | P10037, 1 interactor |
STRINGi | 10116.ENSRNOP00000000905 |
PTM databases
iPTMneti | P10037 |
PhosphoSitePlusi | P10037 |
Proteomic databases
PRIDEi | P10037 |
Genome annotation databases
Ensembli | ENSRNOT00000000905; ENSRNOP00000000905; ENSRNOG00000000715 [P10037-1] |
GeneIDi | 25517 |
UCSCi | RGD:3367 rat [P10037-1] |
Organism-specific databases
CTDi | 5449 |
RGDi | 3367 Pou1f1 |
Phylogenomic databases
GeneTreei | ENSGT00940000158913 |
InParanoidi | P10037 |
OMAi | FPEHALS |
OrthoDBi | 797288at2759 |
PhylomeDBi | P10037 |
TreeFami | TF316413 |
Miscellaneous databases
EvolutionaryTracei | P10037 |
PROi | PR:P10037 |
Gene expression databases
Bgeei | ENSRNOG00000000715 Expressed in 4 organ(s), highest expression level in brain |
Genevisiblei | P10037 RN |
Family and domain databases
CDDi | cd00086 homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057 Homeobox-like_sf IPR017970 Homeobox_CS IPR001356 Homeobox_dom IPR010982 Lambda_DNA-bd_dom_sf IPR015586 Pit_1 IPR013847 POU IPR000327 POU_dom |
PANTHERi | PTHR11636:SF84 PTHR11636:SF84, 1 hit |
Pfami | View protein in Pfam PF00046 Homeodomain, 1 hit PF00157 Pou, 1 hit |
PRINTSi | PR00028 POUDOMAIN |
SMARTi | View protein in SMART SM00389 HOX, 1 hit SM00352 POU, 1 hit |
SUPFAMi | SSF46689 SSF46689, 1 hit SSF47413 SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027 HOMEOBOX_1, 1 hit PS50071 HOMEOBOX_2, 1 hit PS00035 POU_1, 1 hit PS00465 POU_2, 1 hit PS51179 POU_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIT1_RAT | |
Accessioni | P10037Primary (citable) accession number: P10037 Secondary accession number(s): Q63431 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | April 27, 2001 | |
Last modified: | November 13, 2019 | |
This is version 192 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references