Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Relaxosome protein TraM

Gene

traM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Cooperatively binds 3 regions in the F plasmid oriT locus; 2 are required for autoregulation while the other is required for plasmid transfer. Bends oriT DNA less than 50 degrees. Plasmid specificity is conferred by the TraD-TraM pair.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processConjugation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Relaxosome protein TraM
Gene namesi
Name:traM
Ordered Locus Names:ECOK12F071
Encoded oniPlasmid F12 Publications
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of conjugative DNA transfer.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5N → D: Loss of all functions. 1 Publication1
Mutagenesisi76K → E: 100,000-fold decrease in conjugation efficiency, but tetramerizes and binds DNA normally. 1 Publication1
Mutagenesisi88E → L or Q: Increased homotetramer stability. 1 Publication1
Mutagenesisi99K → E: 100,000-fold decrease in conjugation efficiency, but tetramerizes and binds DNA normally. Binds less well to TraD. May be dominant over wild-type. Partially rescued by a TraD E-712 mutation. 2 Publications1
Mutagenesisi106V → A: 2000-fold decrease in conjugation efficiency, but tetramerizes and binds DNA normally. 1 Publication1
Mutagenesisi110R → E: 33,000-fold decrease in conjugation efficiency, but tetramerize and bind DNA normally. 1 Publication1
Mutagenesisi121F → S: Alters oligomerization, probably more dimers than tetramers. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000684671 – 127Relaxosome protein TraMAdd BLAST127

Proteomic databases

PRIDEiP10026

Expressioni

Inductioni

Regulates its own expression from two promoters, requires TraY as well as expression of the tra operon for maximal transcription. TraM is in a monocistonic operon.1 Publication

Interactioni

Subunit structurei

Homotetramer. 2 homotetramers cooperatively bind to DNA although they do not contact each other; cooperativity is achieved by DNA kinking and unwinding (Probable). Part of the relaxosome, a complex composed of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to the F plasmid origin of transfer (oriT) in a site- and sequence-specific manner. Interacts with TraD. Also interacts with TraY.Curated6 Publications

Protein-protein interaction databases

DIPiDIP-27653N

Structurei

Secondary structure

1127
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP10026
SMRiP10026
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10026

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili63 – 891 PublicationAdd BLAST27

Sequence similaritiesi

Belongs to the relaxosome TraM family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

CDDicd14804 Tra_M, 1 hit
InterProiView protein in InterPro
IPR010992 IHF-like_DNA-bd_dom_sf
IPR007925 TRelaxosome_TraM
PfamiView protein in Pfam
PF05261 Tra_M, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD008204 T4SS_TraM, 1 hit
SUPFAMiSSF47729 SSF47729, 1 hit

Sequencei

Sequence statusi: Complete.

P10026-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKVNLYISN DAYEKINAII EKRRQEGARE KDVSFSATAS MLLELGLRVH
60 70 80 90 100
EAQMERKESA FNQTEFNKLL LECVVKTQSS VAKILGIESL SPHVSGNSKF
110 120
EYANMVEDIR EKVSSEMERF FPKNDDE
Length:127
Mass (Da):14,507
Last modified:July 1, 1989 - v1
Checksum:iFC4642125B7E7CAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01147 Genomic DNA Translation: AAA24907.1
X00545 Genomic DNA Translation: CAA25216.1
U01159 Genomic DNA Translation: AAC44191.1
AP001918 Genomic DNA Translation: BAA97941.1
PIRiA29331 JCECMF
RefSeqiNP_061450.1, NC_002483.1
WP_001151527.1, NZ_CP014273.1

Genome annotation databases

GeneIDi1263565
PATRICifig|83333.107.peg.642

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01147 Genomic DNA Translation: AAA24907.1
X00545 Genomic DNA Translation: CAA25216.1
U01159 Genomic DNA Translation: AAC44191.1
AP001918 Genomic DNA Translation: BAA97941.1
PIRiA29331 JCECMF
RefSeqiNP_061450.1, NC_002483.1
WP_001151527.1, NZ_CP014273.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G7OX-ray1.40A58-127[»]
2G9EX-ray1.80A58-127[»]
3D8AX-ray2.55A/B/C/D/E/F/G/H58-127[»]
4QPOX-ray2.00A/B/C/D2-54[»]
4QPQX-ray3.11A/B/C/D/E/F/G/H2-54[»]
ProteinModelPortaliP10026
SMRiP10026
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-27653N

Proteomic databases

PRIDEiP10026

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1263565
PATRICifig|83333.107.peg.642

Miscellaneous databases

EvolutionaryTraceiP10026
PROiPR:P10026

Family and domain databases

CDDicd14804 Tra_M, 1 hit
InterProiView protein in InterPro
IPR010992 IHF-like_DNA-bd_dom_sf
IPR007925 TRelaxosome_TraM
PfamiView protein in Pfam
PF05261 Tra_M, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD008204 T4SS_TraM, 1 hit
SUPFAMiSSF47729 SSF47729, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRAM1_ECOLI
AccessioniPrimary (citable) accession number: P10026
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again