UniProtKB - P10026 (TRAM1_ECOLI)
Relaxosome protein TraM
traM
Functioni
Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Cooperatively binds 3 regions in the F plasmid oriT locus; 2 are required for autoregulation while the other is required for plasmid transfer. Bends oriT DNA less than 50 degrees. Plasmid specificity is conferred by the TraD-TraM pair.
4 PublicationsGO - Molecular functioni
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- conjugation Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Conjugation, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Relaxosome protein TraM |
Gene namesi | Name:traM Ordered Locus Names:ECOK12F071 |
Encoded oni | Plasmid F12 Publications |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 5 | N → D: Loss of all functions. 1 Publication | 1 | |
Mutagenesisi | 76 | K → E: 100,000-fold decrease in conjugation efficiency, but tetramerizes and binds DNA normally. 1 Publication | 1 | |
Mutagenesisi | 88 | E → L or Q: Increased homotetramer stability. 1 Publication | 1 | |
Mutagenesisi | 99 | K → E: 100,000-fold decrease in conjugation efficiency, but tetramerizes and binds DNA normally. Binds less well to TraD. May be dominant over wild-type. Partially rescued by a TraD E-712 mutation. 2 Publications | 1 | |
Mutagenesisi | 106 | V → A: 2000-fold decrease in conjugation efficiency, but tetramerizes and binds DNA normally. 1 Publication | 1 | |
Mutagenesisi | 110 | R → E: 33,000-fold decrease in conjugation efficiency, but tetramerize and bind DNA normally. 1 Publication | 1 | |
Mutagenesisi | 121 | F → S: Alters oligomerization, probably more dimers than tetramers. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068467 | 1 – 127 | Relaxosome protein TraMAdd BLAST | 127 |
Proteomic databases
PRIDEi | P10026 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homotetramer. 2 homotetramers cooperatively bind to DNA although they do not contact each other; cooperativity is achieved by DNA kinking and unwinding (Probable). Part of the relaxosome, a complex composed of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to the F plasmid origin of transfer (oriT) in a site- and sequence-specific manner.
Interacts with TraD.
Also interacts with TraY.
Curated6 PublicationsProtein-protein interaction databases
DIPi | DIP-27653N |
Structurei
Secondary structure
3D structure databases
SMRi | P10026 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P10026 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 63 – 89 | 1 PublicationAdd BLAST | 27 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilFamily and domain databases
CDDi | cd14804, Tra_M, 1 hit |
Gene3Di | 1.10.10.450, 1 hit |
InterProi | View protein in InterPro IPR010992, IHF-like_DNA-bd_dom_sf IPR042073, TraM_DNA-bd IPR007925, TRelaxosome_TraM |
Pfami | View protein in Pfam PF05261, Tra_M, 1 hit |
SUPFAMi | SSF47729, SSF47729, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAKVNLYISN DAYEKINAII EKRRQEGARE KDVSFSATAS MLLELGLRVH
60 70 80 90 100
EAQMERKESA FNQTEFNKLL LECVVKTQSS VAKILGIESL SPHVSGNSKF
110 120
EYANMVEDIR EKVSSEMERF FPKNDDE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01147 Genomic DNA Translation: AAA24907.1 X00545 Genomic DNA Translation: CAA25216.1 U01159 Genomic DNA Translation: AAC44191.1 AP001918 Genomic DNA Translation: BAA97941.1 |
PIRi | A29331, JCECMF |
RefSeqi | NP_061450.1, NC_002483.1 WP_001151527.1, NZ_CP014273.1 |
Genome annotation databases
PATRICi | fig|83333.107.peg.642 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01147 Genomic DNA Translation: AAA24907.1 X00545 Genomic DNA Translation: CAA25216.1 U01159 Genomic DNA Translation: AAC44191.1 AP001918 Genomic DNA Translation: BAA97941.1 |
PIRi | A29331, JCECMF |
RefSeqi | NP_061450.1, NC_002483.1 WP_001151527.1, NZ_CP014273.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2G7O | X-ray | 1.40 | A | 58-127 | [»] | |
2G9E | X-ray | 1.80 | A | 58-127 | [»] | |
3D8A | X-ray | 2.55 | A/B/C/D/E/F/G/H | 58-127 | [»] | |
4QPO | X-ray | 2.00 | A/B/C/D | 2-54 | [»] | |
4QPQ | X-ray | 3.11 | A/B/C/D/E/F/G/H | 2-54 | [»] | |
SMRi | P10026 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-27653N |
Proteomic databases
PRIDEi | P10026 |
Genome annotation databases
PATRICi | fig|83333.107.peg.642 |
Miscellaneous databases
EvolutionaryTracei | P10026 |
PROi | PR:P10026 |
Family and domain databases
CDDi | cd14804, Tra_M, 1 hit |
Gene3Di | 1.10.10.450, 1 hit |
InterProi | View protein in InterPro IPR010992, IHF-like_DNA-bd_dom_sf IPR042073, TraM_DNA-bd IPR007925, TRelaxosome_TraM |
Pfami | View protein in Pfam PF05261, Tra_M, 1 hit |
SUPFAMi | SSF47729, SSF47729, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRAM1_ECOLI | |
Accessioni | P10026Primary (citable) accession number: P10026 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | September 29, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, PlasmidDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families