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UniProtKB - P0DUB6 (AMY1A_HUMAN)
Protein
Alpha-amylase 1A
Gene
AMY1A
Organism
Homo sapiens (Human)
Status
Functioni
Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308).
Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308).
1 PublicationCaution
Three distinct genes (AMY1A, AMY1B and AMY1C), located in a gene cluster on 1p21, encode proteins sharing the same peptidic sequence.Curated
Catalytic activityi
- Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication EC:3.2.1.1
Cofactori
Protein has several cofactor binding sites:- Ca2+2 PublicationsNote: Binds 1 Ca2+ ion per subunit.2 Publications
- chloride2 PublicationsNote: Binds 1 Cl- ion per subunit.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 115 | CalciumCombined sources2 Publications | 1 | |
Metal bindingi | 173 | Calcium; via carbonyl oxygenCombined sources2 Publications | 1 | |
Metal bindingi | 182 | CalciumCombined sources2 Publications | 1 | |
Binding sitei | 210 | ChlorideCombined sources2 Publications | 1 | |
Active sitei | 212 | Nucleophile | 1 | |
Metal bindingi | 216 | Calcium; via carbonyl oxygenCombined sources2 Publications | 1 | |
Active sitei | 248 | Proton donor | 1 | |
Binding sitei | 313 | ChlorideCombined sources2 Publications | 1 | |
Sitei | 315 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 352 | ChlorideCombined sources2 Publications | 1 |
GO - Molecular functioni
- alpha-amylase activity Source: UniProtKB
- alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
- calcium ion binding Source: UniProtKB
- chloride ion binding Source: UniProtKB
GO - Biological processi
- oligosaccharide metabolic process Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Calcium, Chloride, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | P04745 |
Reactomei | R-HSA-189085, Digestion of dietary carbohydrate |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-amylase 1A (EC:3.2.1.11 Publication)Alternative name(s): 1,4-alpha-D-glucan glucanohydrolase 1 Salivary alpha-amylase |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:474, AMY1A |
MIMi | 104702, gene |
neXtProti | NX_P0DUB6 |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular space Source: UniProtKB
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 276 277 278 |
OpenTargetsi | ENSG00000187733 |
Chemistry databases
ChEMBLi | CHEMBL2478 |
Genetic variation databases
BioMutai | AMY1A |
DMDMi | 1351933 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | Sequence analysisAdd BLAST | 15 | |
ChainiPRO_0000001401 | 16 – 511 | Alpha-amylase 1AAdd BLAST | 496 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 16 | Pyrrolidone carboxylic acidBy similarity | 1 | |
Disulfide bondi | 43 ↔ 101 | Combined sources2 Publications | ||
Disulfide bondi | 85 ↔ 130 | Combined sources2 Publications | ||
Disulfide bondi | 156 ↔ 175 | Combined sources2 Publications | ||
Modified residuei | 365 | Deamidated asparagine; partial1 Publication | 1 | |
Disulfide bondi | 393 ↔ 399 | Combined sources2 Publications | ||
Modified residuei | 427 | Deamidated asparagine; partial; alternate1 Publication | 1 | |
Glycosylationi | 427 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 465 ↔ 477 | Combined sources2 Publications | ||
Modified residuei | 474 | Deamidated asparagine; partial1 Publication | 1 | |
Glycosylationi | 476 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acidProteomic databases
jPOSTi | P04745 |
PaxDbi | P04745 |
PTM databases
GlyGeni | P0DUB6, 2 sites |
iPTMneti | P04745 |
PhosphoSitePlusi | P0DUB6 |
Interactioni
Subunit structurei
Monomer.
Chemistry databases
BindingDBi | P0DUB6 |
Miscellaneous databases
RNActi | P04745, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0DUB6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04745 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 13 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2212, Eukaryota |
GeneTreei | ENSGT00940000154802 |
HOGENOMi | CLU_013336_2_1_1 |
InParanoidi | P04745 |
OMAi | TWVETHD |
OrthoDBi | 665362at2759 |
TreeFami | TF312850 |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR031319, A-amylase_C IPR006046, Alpha_amylase IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit |
PRINTSi | PR00110, ALPHAAMYLASE |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit SM00632, Aamy_C, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0DUB6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLFWLLFTI GFCWAQYSSN TQQGRTSIVH LFEWRWVDIA LECERYLAPK
60 70 80 90 100
GFGGVQVSPP NENVAIHNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD
160 170 180 190 200
FNDGKCKTGS GDIENYNDAT QVRDCRLSGL LDLALGKDYV RSKIAEYMNH
210 220 230 240 250
LIDIGVAGFR IDASKHMWPG DIKAILDKLH NLNSNWFPEG SKPFIYQEVI
260 270 280 290 300
DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG
310 320 330 340 350
FMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF
360 370 380 390 400
TRVMSSYRWP RYFENGKDVN DWVGPPNDNG VTKEVTINPD TTCGNDWVCE
410 420 430 440 450
HRWRQIRNMV NFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWT
460 470 480 490 500
FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED
510
PFIAIHAESK L
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 285 | N → T in AAA57345 (PubMed:2442579).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18786 , M18715, M18717, M18719, M18721, M18723, M18725, M18727, M18784 Genomic DNA Translation: AAA52279.1 AK292341 mRNA Translation: BAF85030.1 AC105272 Genomic DNA No translation available. BC063129 mRNA Translation: AAH63129.1 BC069347 mRNA Translation: AAH69347.1 BC069463 mRNA Translation: AAH69463.1 BC092444 mRNA Translation: AAH92444.1 BC132985 mRNA Translation: AAI32986.1 BC132987 mRNA Translation: AAI32988.1 BC132995 mRNA Translation: AAI32996.1 BC132997 mRNA Translation: AAI32998.1 M18671 Genomic DNA Translation: AAA58368.1 M18674 Genomic DNA Translation: AAA16183.2 M19233, M17883 Genomic DNA Translation: AAA57345.1 Different termination. M17884 Genomic DNA No translation available. |
CCDSi | CCDS30782.1 |
PIRi | A91543, ALHUS |
RefSeqi | NP_001008219.1, NM_001008218.1 NP_001008220.1, NM_001008219.2 NP_001008222.1, NM_001008221.1 NP_001333709.1, NM_001346780.1 NP_004029.2, NM_004038.3 XP_011539564.1, XM_011541262.1 XP_016856547.1, XM_017001058.1 |
Genome annotation databases
Ensembli | ENST00000370083; ENSP00000359100; ENSG00000237763 |
GeneIDi | 276 277 278 |
KEGGi | hsa:276 hsa:277 hsa:278 |
MANE-Selecti | ENST00000330330.10; ENSP00000330484.5; NM_001008218.2; NP_001008219.1 ENST00000370083.9; ENSP00000359100.4; NM_004038.4; NP_004029.2 ENST00000622339.5; ENSP00000481450.2; NM_001008219.3; NP_001008220.1 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Amylase entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18786 , M18715, M18717, M18719, M18721, M18723, M18725, M18727, M18784 Genomic DNA Translation: AAA52279.1 AK292341 mRNA Translation: BAF85030.1 AC105272 Genomic DNA No translation available. BC063129 mRNA Translation: AAH63129.1 BC069347 mRNA Translation: AAH69347.1 BC069463 mRNA Translation: AAH69463.1 BC092444 mRNA Translation: AAH92444.1 BC132985 mRNA Translation: AAI32986.1 BC132987 mRNA Translation: AAI32988.1 BC132995 mRNA Translation: AAI32996.1 BC132997 mRNA Translation: AAI32998.1 M18671 Genomic DNA Translation: AAA58368.1 M18674 Genomic DNA Translation: AAA16183.2 M19233, M17883 Genomic DNA Translation: AAA57345.1 Different termination. M17884 Genomic DNA No translation available. |
CCDSi | CCDS30782.1 |
PIRi | A91543, ALHUS |
RefSeqi | NP_001008219.1, NM_001008218.1 NP_001008220.1, NM_001008219.2 NP_001008222.1, NM_001008221.1 NP_001333709.1, NM_001346780.1 NP_004029.2, NM_004038.3 XP_011539564.1, XM_011541262.1 XP_016856547.1, XM_017001058.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1C8Q | X-ray | 2.30 | A | 16-511 | [»] | |
1JXJ | X-ray | 1.99 | A | 16-511 | [»] | |
1JXK | X-ray | 1.90 | A | 16-511 | [»] | |
1MFU | X-ray | 2.00 | A | 17-511 | [»] | |
1MFV | X-ray | 2.00 | A | 17-511 | [»] | |
1NM9 | X-ray | 2.10 | A | 16-511 | [»] | |
1Q4N | X-ray | 2.07 | X | 16-511 | [»] | |
1SMD | X-ray | 1.60 | A | 17-511 | [»] | |
1XV8 | X-ray | 3.00 | A/B | 16-511 | [»] | |
1Z32 | X-ray | 1.60 | X | 16-511 | [»] | |
3BLK | X-ray | 2.00 | A | 16-511 | [»] | |
3BLP | X-ray | 1.60 | X | 16-511 | [»] | |
3DHP | X-ray | 1.50 | A | 16-511 | [»] | |
SMRi | P0DUB6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
BindingDBi | P0DUB6 |
ChEMBLi | CHEMBL2478 |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
PTM databases
GlyGeni | P0DUB6, 2 sites |
iPTMneti | P04745 |
PhosphoSitePlusi | P0DUB6 |
Genetic variation databases
BioMutai | AMY1A |
DMDMi | 1351933 |
Proteomic databases
jPOSTi | P04745 |
PaxDbi | P04745 |
Genome annotation databases
Ensembli | ENST00000370083; ENSP00000359100; ENSG00000237763 |
GeneIDi | 276 277 278 |
KEGGi | hsa:276 hsa:277 hsa:278 |
MANE-Selecti | ENST00000330330.10; ENSP00000330484.5; NM_001008218.2; NP_001008219.1 ENST00000370083.9; ENSP00000359100.4; NM_004038.4; NP_004029.2 ENST00000622339.5; ENSP00000481450.2; NM_001008219.3; NP_001008220.1 |
Organism-specific databases
CTDi | 276 277 278 |
DisGeNETi | 276 277 278 |
GeneCardsi | AMY1A |
HGNCi | HGNC:474, AMY1A |
MIMi | 104702, gene |
neXtProti | NX_P0DUB6 |
OpenTargetsi | ENSG00000187733 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2212, Eukaryota |
GeneTreei | ENSGT00940000154802 |
HOGENOMi | CLU_013336_2_1_1 |
InParanoidi | P04745 |
OMAi | TWVETHD |
OrthoDBi | 665362at2759 |
TreeFami | TF312850 |
Enzyme and pathway databases
PathwayCommonsi | P04745 |
Reactomei | R-HSA-189085, Digestion of dietary carbohydrate |
Miscellaneous databases
EvolutionaryTracei | P04745 |
GeneWikii | AMY1A |
RNActi | P04745, protein |
SOURCEi | Search... |
Gene expression databases
Genevisiblei | P04745, HS |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR031319, A-amylase_C IPR006046, Alpha_amylase IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit |
PRINTSi | PR00110, ALPHAAMYLASE |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit SM00632, Aamy_C, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AMY1A_HUMAN | |
Accessioni | P0DUB6Primary (citable) accession number: P0DUB6 Secondary accession number(s): A6NJS5 Q5T083 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 2, 2020 |
Last sequence update: | December 2, 2020 | |
Last modified: | February 23, 2022 | |
This is version 6 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families