UniProtKB - P0DTC9 (NCAP_SARS2)
Nucleoprotein
N
Functioni
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (By similarity).
UniRule annotationBy similarityMay induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
1 PublicationGO - Molecular functioni
- identical protein binding Source: IntAct
- RNA binding Source: UniProtKB-UniRule
GO - Biological processi
- negative regulation of interferon-beta production Source: ComplexPortal
- positive regulation of NLRP3 inflammasome complex assembly Source: UniProtKB
- viral RNA genome packaging Source: ComplexPortal
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-HSA-9694322, Virion Assembly and Release R-HSA-9694614, Attachment and Entry R-HSA-9694631, Maturation of nucleoprotein R-HSA-9694635, Translation of Structural Proteins R-HSA-9694786, Transcription of SARS-CoV-2 sgRNAs |
SIGNORi | P0DTC9 |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinUniRule annotationShort name: N Alternative name(s): Nucleocapsid proteinUniRule annotation Short name: NCUniRule annotation Short name: Protein NUniRule annotation |
Gene namesi | Name:NUniRule annotation |
Organismi | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) |
Taxonomic identifieri | 2697049 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Sarbecovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion UniRule annotation
- Host cytoplasm 2 Publications Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.UniRule annotation
Keywords - Cellular componenti
Host cytoplasm, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 109 | Y → A: Significant decrease of RNA binding capacity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000449656 | 1 – 419 | NucleoproteinAdd BLAST | 419 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 176 | Phosphoserine; by hostUniRule annotation | 1 |
Post-translational modificationi
Keywords - PTMi
ADP-ribosylation, PhosphoproteinInteractioni
Subunit structurei
Homooligomer. Both monomeric and oligomeric forms interact with RNA.
Interacts with protein M.
Interacts with protein E.
Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection (By similarity). May interact with host NLRP3 (PubMed:34341353).
UniRule annotationBy similarity1 PublicationBinary interactionsi
P0DTC9
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4383847, 466 interactors |
ComplexPortali | CPX-5686, SARS-CoV-2 nucleocapsid complex |
IntActi | P0DTC9, 518 interactors |
Structurei
Secondary structure
3D structure databases
BMRBi | P0DTC9 |
SMRi | P0DTC9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 48 – 174 | CoV N NTDPROSITE-ProRule annotation1 PublicationAdd BLAST | 127 | |
Domaini | 247 – 364 | CoV N CTDPROSITE-ProRule annotationAdd BLAST | 118 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 51 | DisorderedSequence analysisAdd BLAST | 51 | |
Regioni | 41 – 186 | RNA-bindingUniRule annotationAdd BLAST | 146 | |
Regioni | 63 – 83 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 168 – 212 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 233 – 266 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 258 – 361 | DimerizationUniRule annotationAdd BLAST | 104 | |
Regioni | 260 – 340 | Putative NLRP3 binding1 PublicationAdd BLAST | 81 | |
Regioni | 361 – 391 | DisorderedSequence analysisAdd BLAST | 31 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 33 | Polar residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 177 – 208 | Polar residuesSequence analysisAdd BLAST | 32 | |
Compositional biasi | 233 – 251 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 362 – 382 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Family and domain databases
CDDi | cd21595, CoV_N-CTD, 1 hit cd21554, CoV_N-NTD, 1 hit |
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR044344, N_prot_C_CoV IPR044345, N_prot_N_CoV IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSDNGPQNQR NAPRITFGGP SDSTGSNQNG ERSGARSKQR RPQGLPNNTA
60 70 80 90 100
SWFTALTQHG KEDLKFPRGQ GVPINTNSSP DDQIGYYRRA TRRIRGGDGK
110 120 130 140 150
MKDLSPRWYF YYLGTGPEAG LPYGANKDGI IWVATEGALN TPKDHIGTRN
160 170 180 190 200
PANNAAIVLQ LPQGTTLPKG FYAEGSRGGS QASSRSSSRS RNSSRNSTPG
210 220 230 240 250
SSRGTSPARM AGNGGDAALA LLLLDRLNQL ESKMSGKGQQ QQGQTVTKKS
260 270 280 290 300
AAEASKKPRQ KRTATKAYNV TQAFGRRGPE QTQGNFGDQE LIRQGTDYKH
310 320 330 340 350
WPQIAQFAPS ASAFFGMSRI GMEVTPSGTW LTYTGAIKLD DKDPNFKDQV
360 370 380 390 400
ILLNKHIDAY KTFPPTEPKK DKKKKADETQ ALPQRQKKQQ TVTLLPAADL
410
DDFSKQLQQS MSSADSTQA
Polymorphismi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 2 – 3 | SD → Y in strain: Eta/B.1.525. Curated | 2 | |
Natural varianti | 3 | D → L in strain: Alpha/B.1.1.7. 1 Publication | 1 | |
Natural varianti | 12 | A → G in strain: Eta/B.1.525. | 1 | |
Natural varianti | 13 | P → L in strain: Lambda/C.37, Omicron/BA.1, Omicron/BA.2. Curated | 1 | |
Natural varianti | 31 – 33 | Missing in strain: Omicron/BA.1, Omicron/BA.2. Curated | 3 | |
Natural varianti | 63 | D → G in strain: Delta/B.1.617.2. Curated | 1 | |
Natural varianti | 80 | P → R in strain: Gamma/P.1. Curated | 1 | |
Natural varianti | 119 | A → S in strain: Zeta/P.2. Curated | 1 | |
Natural varianti | 203 – 204 | RG → KR in strain: Alpha/B.1.1.7, Gamma/P.1, Zeta/P.2, Theta/P.3, Lambda/C.37, Omicron/BA.1, Omicron/BA.2. Curated | 2 | |
Natural varianti | 203 | R → M in strain: Delta/B.1.617.2, Kappa/B.1.617.1. Curated | 1 | |
Natural varianti | 205 | T → I in strain: Beta/B.1.351, Epsilon/B.1.429, Eta/B.1.525 and Mu/B.1.621. Curated | 1 | |
Natural varianti | 214 | G → C in strain: Lambda/C.37. | 1 | |
Natural varianti | 234 | M → I in strain: Zeta/P.2. Curated | 1 | |
Natural varianti | 235 | S → F in strain: Alpha/B.1.1.7. 1 Publication | 1 | |
Natural varianti | 377 | D → Y in strain: Delta/B.1.617.2, Kappa/B.1.617.1. Curated | 1 | |
Natural varianti | 413 | S → R in strain: Omicron/BA.2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | MN908947 Genomic RNA Translation: QHD43423.2 |
RefSeqi | YP_009724397.2, NC_045512.2 |
Genome annotation databases
GeneIDi | 43740575 |
KEGGi | vg:43740575 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | MN908947 Genomic RNA Translation: QHD43423.2 |
RefSeqi | YP_009724397.2, NC_045512.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6M3M | X-ray | 2.70 | A/B/C/D | 41-174 | [»] | |
6VYO | X-ray | 1.70 | A/B/C/D | 47-173 | [»] | |
6WJI | X-ray | 2.05 | A/B/C/D/E/F | 257-364 | [»] | |
6WKP | X-ray | 2.67 | A/B/C/D | 47-173 | [»] | |
6WZO | X-ray | 1.42 | A/B/C/D | 247-364 | [»] | |
6WZQ | X-ray | 1.45 | A/B/C/D | 247-364 | [»] | |
6YI3 | NMR | - | A | 44-180 | [»] | |
6YUN | X-ray | 1.44 | A/B | 249-364 | [»] | |
6ZCO | X-ray | 1.36 | A | 247-364 | [»] | |
7ACS | NMR | - | A | 44-180 | [»] | |
7ACT | NMR | - | A | 44-180 | [»] | |
7C22 | X-ray | 2.00 | A/B/C/D | 248-364 | [»] | |
7CDZ | X-ray | 1.80 | A/B/C/D | 44-174 | [»] | |
7CE0 | X-ray | 1.50 | A/B/C/D | 255-364 | [»] | |
7CR5 | X-ray | 2.08 | A | 41-174 | [»] | |
7DE1 | X-ray | 2.00 | A/B | 250-364 | [»] | |
7F2B | X-ray | 2.00 | A/B | 257-362 | [»] | |
7F2E | X-ray | 3.10 | A/B/C/D/E/F/G/H/I/J/K/L | 255-362 | [»] | |
7KGO | X-ray | 2.15 | C | 351-359 | [»] | |
7KGP | X-ray | 1.40 | C | 316-324 | [»] | |
7KGQ | X-ray | 1.34 | C | 222-230 | [»] | |
7KGR | X-ray | 1.55 | C | 159-167 | [»] | |
7KGS | X-ray | 1.58 | C | 138-146 | [»] | |
7KGT | X-ray | 1.90 | C | 226-234 | [»] | |
7LGD | X-ray | 2.88 | E/F | 105-113 | [»] | |
7LTU | X-ray | 1.12 | A/B | 217-222 | [»] | |
7LUX | X-ray | 1.30 | A | 217-222 | [»] | |
7LUZ | X-ray | 1.10 | A | 243-248 | [»] | |
7LV2 | X-ray | 1.30 | A | 179-184 | [»] | |
7N0I | X-ray | 2.20 | A/B/C/D/E/F/G/H | 269-364 | [»] | |
7N0R | X-ray | 1.42 | A/B | 49-174 | [»] | |
7N3C | X-ray | 1.82 | C | 47-173 | [»] | |
7N3D | X-ray | 1.53 | C | 47-173 | [»] | |
7R98 | X-ray | 2.51 | A/B/C | 49-174 | [»] | |
7VNU | X-ray | 1.95 | A/B/C/D | 47-174 | [»] | |
BMRBi | P0DTC9 | |||||
SMRi | P0DTC9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4383847, 466 interactors |
ComplexPortali | CPX-5686, SARS-CoV-2 nucleocapsid complex |
IntActi | P0DTC9, 518 interactors |
Protocols and materials databases
ABCDi | P0DTC9, 26 sequenced antibodies |
DNASUi | 43740575 |
Genome annotation databases
GeneIDi | 43740575 |
KEGGi | vg:43740575 |
Enzyme and pathway databases
Reactomei | R-HSA-9694322, Virion Assembly and Release R-HSA-9694614, Attachment and Entry R-HSA-9694631, Maturation of nucleoprotein R-HSA-9694635, Translation of Structural Proteins R-HSA-9694786, Transcription of SARS-CoV-2 sgRNAs |
SIGNORi | P0DTC9 |
Miscellaneous databases
PROi | PR:P0DTC9 |
Family and domain databases
CDDi | cd21595, CoV_N-CTD, 1 hit cd21554, CoV_N-NTD, 1 hit |
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR044344, N_prot_C_CoV IPR044345, N_prot_N_CoV IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_SARS2 | |
Accessioni | P0DTC9Primary (citable) accession number: P0DTC9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 22, 2020 |
Last sequence update: | April 22, 2020 | |
Last modified: | May 25, 2022 | |
This is version 11 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families