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UniProtKB - P0DTC7 (NS7A_SARS2)
Protein
ORF7a protein
Gene
7a
Organism
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Status
Functioni
Plays a role as antagonist of host tetherin (BST2), disrupting its antiviral effect. Acts by binding to BST2 and sequestering it to perinuclear region, thereby preventing its antiviral function at cell membrane.
1 PublicationMiscellaneous
Variant B.1.1.7 is also called Variant Of Concern (VOC) 202012/01, Variant Under Investigation (VUI) 202012/01, or 20B/501Y.V1.1 Publication
GO - Biological processi
- modulation by virus of host G0/G1 transition checkpoint Source: UniProtKB-KW
- suppression by virus of host tetherin activity Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
Keywordsi
Enzyme and pathway databases
Reactomei | R-HSA-9694322, Virion Assembly and Release R-HSA-9694614, Attachment and Entry |
Names & Taxonomyi
Protein namesi | Recommended name: ORF7a proteinShort name: ORF7a Alternative name(s): Accessory protein 7a Protein U122 Protein X4 |
Gene namesi | ORF Names:7a |
Organismi | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) |
Taxonomic identifieri | 2697049 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Sarbecovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- host perinuclear region 1 Publication
- Virion By similarity
- Host endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity
- Host endoplasmic reticulum-Golgi intermediate compartment membrane By similarity; Single-pass type I membrane protein By similarity
- Host Golgi apparatus membrane By similarity; Single-pass membrane protein By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 96 – 116 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Host cytoplasm, Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | K → A: No effect on ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 32 | K → A: No effect on ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 53 | K → A: No effect on ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 72 | K → A: No effect on ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 85 | K → A: No effect on ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 117 | K → A: No effect on ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 119 | K → A: Complete loss of ubiquitination. Partial loss of interferon pathway inhibition. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | Sequence analysisAdd BLAST | 15 | |
ChainiPRO_0000449654 | 16 – 121 | ORF7a proteinSequence analysisAdd BLAST | 106 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 23 ↔ 58 | 2 Publications | ||
Disulfide bondi | 35 ↔ 67 | 2 Publications | ||
Cross-linki | 119 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Post-translational modificationi
Poly-ubiquitinated by host with K63-linked polyubiquitin chains.1 Publication
Keywords - PTMi
Disulfide bond, Isopeptide bond, Ubl conjugationInteractioni
Subunit structurei
Interacts with host BST2 (PubMed:33930332).
Interacts with the spike glycoprotein (By similarity).
Interacts with M protein (By similarity).
Interacts with E protein (By similarity).
Interacts with the ORF3a protein (By similarity).
By similarity1 PublicationBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 4383872, 1103 interactors |
IntActi | P0DTC7, 109 interactors |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0DTC7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 16 – 81 | X4ePROSITE-ProRule annotationAdd BLAST | 66 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 117 – 121 | Di-lysine motifBy similarity | 5 |
Domaini
The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.By similarity
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd21684, ORF7a_SARS-CoV-2-like, 1 hit |
Gene3Di | 2.60.40.1550, 1 hit |
InterProi | View protein in InterPro IPR044390, ORF7a_SARS-CoV-2-like IPR014888, ORF7a_SARS-CoV-like IPR044871, ORF7a_SARS-CoV-like_X4e IPR036495, ORF7a_sf_CoV |
Pfami | View protein in Pfam PF08779, bCoV_NS7A, 1 hit |
SUPFAMi | SSF117066, SSF117066, 1 hit |
PROSITEi | View protein in PROSITE PS51919, X4E, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0DTC7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIILFLALI TLATCELYHY QECVRGTTVL LKEPCSSGTY EGNSPFHPLA
60 70 80 90 100
DNKFALTCFS TQFAFACPDG VKHVYQLRAR SVSPKLFIRQ EEVQELYSPI
110 120
FLIVAAIVFI TLCFTLKRKT E
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 14 | T → I in strain: Alpha/B.1.1.7. 1 Publication | 1 | |
Natural varianti | 82 | V → A in strain: Delta/B.1.617.2 and Kappa/B.1.617.1 . Curated | 1 | |
Natural varianti | 120 | T → I in strain; Delta/B.1.617.2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | MN908947 Genomic RNA Translation: QHD43421.1 |
RefSeqi | YP_009724395.1, NC_045512.2 |
Genome annotation databases
GeneIDi | 43740573 |
KEGGi | vg:43740573 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | MN908947 Genomic RNA Translation: QHD43421.1 |
RefSeqi | YP_009724395.1, NC_045512.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6W37 | X-ray | 2.90 | A | 16-82 | [»] | |
7CI3 | X-ray | 2.20 | A | 14-96 | [»] | |
SMRi | P0DTC7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4383872, 1103 interactors |
IntActi | P0DTC7, 109 interactors |
Genome annotation databases
GeneIDi | 43740573 |
KEGGi | vg:43740573 |
Enzyme and pathway databases
Reactomei | R-HSA-9694322, Virion Assembly and Release R-HSA-9694614, Attachment and Entry |
Miscellaneous databases
PROi | PR:P0DTC7 |
Family and domain databases
CDDi | cd21684, ORF7a_SARS-CoV-2-like, 1 hit |
Gene3Di | 2.60.40.1550, 1 hit |
InterProi | View protein in InterPro IPR044390, ORF7a_SARS-CoV-2-like IPR014888, ORF7a_SARS-CoV-like IPR044871, ORF7a_SARS-CoV-like_X4e IPR036495, ORF7a_sf_CoV |
Pfami | View protein in Pfam PF08779, bCoV_NS7A, 1 hit |
SUPFAMi | SSF117066, SSF117066, 1 hit |
PROSITEi | View protein in PROSITE PS51919, X4E, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NS7A_SARS2 | |
Accessioni | P0DTC7Primary (citable) accession number: P0DTC7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 22, 2020 |
Last sequence update: | April 22, 2020 | |
Last modified: | February 23, 2022 | |
This is version 10 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references