UniProtKB - P0DQJ6 (NTA_AVIPU)
Protein
Mu/kappa-theraphotoxin-Ap1a
Gene
N/A
Organism
Avicularia purpurea (Ecuadorian purple pinktoe tarantula)
Status
Functioni
Inhibitor of voltage-gated potassium and sodium channels. Among other potassium channels, it selectively inhibits Kv10.1/KCNH1/EAG1 (IC50=236 nM) by shifting the voltage dependence of channel activation in a depolarising direction, it shows a maximum inhibition of 80% at saturating concentrations, it shows fast on-rates, and is poorly reversible. It also slightly affects channel inactivation, when the membrane is highly depolarised (>+80 mV). It shows similar potency on Nav1.7/SCN9A (IC50=222 nM) and lower potency on Nav1.2/SCN2A (IC50=519 nM).1 Publication
Miscellaneous
Weakly inhibits Nav1.5/SCN5A (IC50=2565), and Kv11.1/KCNH2/ERG1 (IC50=8197 nM).1 Publication
GO - Molecular functioni
- ion channel inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- pathogenesis Source: InterPro
Keywordsi
Molecular function | Ion channel impairing toxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Mu/kappa-theraphotoxin-Ap1a1 PublicationShort name: Mu/kappa-TRTX-Ap1a1 Publication Alternative name(s): Avicularia purpurea |
Organismi | Avicularia purpurea (Ecuadorian purple pinktoe tarantula) |
Taxonomic identifieri | 2652666 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Chelicerata › Arachnida › Araneae › Mygalomorphae › Theraphosidae › Avicularia |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Pharmaceutical usei
Could be used as informative lead for the development of novel antiepileptic drugs, as well as pharmacological tool for probing Kv10.1/KCNH1/EAG1 function.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000448841 | 1 – 36 | Mu/kappa-theraphotoxin-Ap1a1 PublicationAdd BLAST | 36 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 3 ↔ 18 | By similarity | ||
Disulfide bondi | 10 ↔ 23 | By similarity | ||
Disulfide bondi | 17 ↔ 30 | By similarity | ||
Modified residuei | 36 | Phenylalanine amide1 Publication | 1 |
Keywords - PTMi
Amidation, Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.1 Publication
Family & Domainsi
Domaini
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.1 Publication
Sequence similaritiesi
Belongs to the neurotoxin 10 (Hwtx-1) family.Curated
Keywords - Domaini
KnottinFamily and domain databases
InterProi | View protein in InterPro IPR011696, Huwentoxin-1 |
Pfami | View protein in Pfam PF07740, Toxin_12, 1 hit |
i Sequence
Sequence statusi: Complete.
Mass spectrometryi
Molecular mass is 4220.077 Da. Determined by MALDI. Monoisotopic mass.1 Publication
Similar proteinsi
Cross-referencesi
3D structure databases
SMRi | P0DQJ6 |
ModBasei | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR011696, Huwentoxin-1 |
Pfami | View protein in Pfam PF07740, Toxin_12, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NTA_AVIPU | |
Accessioni | P0DQJ6Primary (citable) accession number: P0DQJ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 11, 2019 |
Last sequence update: | December 11, 2019 | |
Last modified: | December 2, 2020 | |
This is version 4 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, PharmaceuticalDocuments
- SIMILARITY comments
Index of protein domains and families