UniProtKB - P0DQH8 (CRF2_CONCF)
Protein
Conorfamide-As2
Gene
N/A
Organism
Conus cancellatus (Cancellate cone) (Conus austini)
Status
Functioni
Conorfamide As2a: this amidated form is active on both ASIC channels and nicotinic acetylcholine receptors (nAChRs) (PubMed:31028742). Potentiates ASIC1a (EC(50)=10.9 µM) (PubMed:31028742). Inhibits desensitization of ASIC1a and to a lesser degree ASIC3 currents, resulting in a sustained opening of channels in the presence of low pH (PubMed:31028742). Inhibits with a slow reversibility human alpha-7/CHRNA7 nAChRs (IC50=689-3867 nM) and with a rapid reversibility human alpha-1-beta-1-delta-epsilon (CHRNA1-CHRNB1-CHRND-CHRNE) nAChRs (IC50=208 nM) (PubMed:31028742).1 Publication
Conorfamide As2b: this non-amidated form is only weakly active on both ASIC channels and nicotinic acetylcholine receptors (nAChRs) (PubMed:31028742). Shows a very weak inhibition of peak current of rat ASIC1a and ASIC3 (PubMed:31028742). No inhibition or very weak desensitization of both rat ASIC1a and ASIC3 are observed (PubMed:31028742). Reversibly inhibits both human alpha-7/CHRNA7 nAChRs (IC50=2971-7036 nM) and human alpha-1-beta-1-delta-epsilon (CHRNA1-CHRNB1-CHRND-CHRNE) nAChRs (IC50=707 nM) (PubMed:31028742).1 Publication
GO - Molecular functioni
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Proton-gated sodium channel impairing toxin, Toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Conorfamide-As21 PublicationAlternative name(s): Conorfamide-As2a1 Publication Short name: CNF-As2aBy similarity Short name: Cono-RFamide-As2aBy similarity Conorfamide-As2b1 Publication Short name: CNF-As2bBy similarity Short name: Cono-RFamide-As2bBy similarity |
Organismi | Conus cancellatus (Cancellate cone) (Conus austini) |
Taxonomic identifieri | 289020 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Spiralia › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Neogastropoda › Conoidea › Conidae › Conus › Dauciconus |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- host cell postsynaptic membrane Source: UniProtKB-KW
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PeptideiPRO_0000447683 | 1 – 12 | Conorfamide-As21 PublicationAdd BLAST | 12 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | Phenylalanine amide; in Conorfamide As2a1 Publication | 1 |
Post-translational modificationi
Both amidated and non-amidated conorfamides As2 are found in the venom. Amidation is important since amidated peptide is more active.1 Publication
Keywords - PTMi
AmidationExpressioni
Tissue specificityi
Expressed by the venom duct.1 Publication
Family & Domainsi
Sequence similaritiesi
Belongs to the FARP (FMRFamide related peptide) family.Curated
i Sequence
Sequence statusi: Complete.
Mass spectrometryi
Molecular mass is 1659.97 Da. Determined by MALDI. Conorfamide As2a.1 Publication
Molecular mass is 1661.02 Da. Determined by MALDI. Conorfamide As2b.1 Publication
Cross-referencesi
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family and domain databases
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CRF2_CONCF | |
Accessioni | P0DQH8Primary (citable) accession number: P0DQH8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 31, 2019 |
Last sequence update: | July 31, 2019 | |
Last modified: | April 22, 2020 | |
This is version 5 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families