UniProtKB - P0DQD4 (O16F_CONCT)
Protein
Omega conotoxin-CVIF
Gene
N/A
Organism
Conus catus (Cat cone)
Status
Functioni
Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels. This toxin blocks N-type calcium channels (Cav2.2/CACNA1B). It shows a higher potency when Cav2.2/CACNA1B is only expressed with the ancillary subunit CACNB3 (IC50=0.1 nM) than on Cav2.2/CACNA1B expressed with the ancillary subunits CACNA2D1 and CACNB3 (IC50=19.9 nM). The Cav2.2/CACNA1B block by this toxin is voltage-independent, whereas the recovery from toxin block is voltage-dependent (PubMed:19892914). There is a low recovery at physiological membrane potential and a high recovery with hyperpolarized potential (PubMed:19892914). This indicates that the toxin has a higher affinity for Cav2.2/CACNA1B in the inactivated state (PubMed:19892914). It is noteworthy that ancillary subunits beta modulate recovery from this toxin block (PubMed:19892914). Cav2.2/CACNA1B expressed with the ancillary subunit CACNB2a (isoform 2a) almost recover completely from this toxin block, whereas an expression with CACNB3 exhibits relatively weak recovery (PubMed:19892914). Inhibition by this toxin of excitatory synaptic transmission is reversible (PubMed:19892914). In vivo, when tested on rat model of persistent pain, this toxin blocks chronic pain behavior (PubMed:19892914).1 Publication
Miscellaneous
Does not show effect on Cav1.2/CACNA1C, Cav1.3/CACNA1D, and Cav2.3/CACNA1E and shows a very little inhibition on Cav2.1/CACNA1A.1 Publication
GO - Molecular functioni
- ion channel inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Calcium channel impairing toxin, Ion channel impairing toxin, Toxin, Voltage-gated calcium channel impairing toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Omega conotoxin-CVIF1 Publication |
Organismi | Conus catus (Cat cone) |
Taxonomic identifieri | 101291 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Neogastropoda › Conoidea › Conidae › Conus › Pionoconus |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | R → K: Alters the kinetics of action and improves reversibility without diminishing conotoxin potency and specificity for Cav2.2/CACNA1B and without diminishing the serum stability. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PeptideiPRO_0000446386 | 1 – 25 | Omega conotoxin-CVIF1 PublicationAdd BLAST | 25 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 1 ↔ 16 | By similarity | ||
Disulfide bondi | 8 ↔ 20 | By similarity | ||
Disulfide bondi | 15 ↔ 25 | By similarity | ||
Modified residuei | 25 | Cysteine amideBy similarity | 1 |
Keywords - PTMi
Amidation, Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom duct.1 Publication
Family & Domainsi
Domaini
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity
The cysteine framework is VI/VII (C-C-CC-C-C).Curated
Sequence similaritiesi
Belongs to the conotoxin O1 superfamily.Curated
Family and domain databases
InterProi | View protein in InterPro IPR012321 Conotoxin_omega-typ_CS |
PROSITEi | View protein in PROSITE PS60004 OMEGA_CONOTOXIN, 1 hit |
i Sequence
Sequence statusi: Complete.
Cross-referencesi
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family and domain databases
InterProi | View protein in InterPro IPR012321 Conotoxin_omega-typ_CS |
PROSITEi | View protein in PROSITE PS60004 OMEGA_CONOTOXIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | O16F_CONCT | |
Accessioni | P0DQD4Primary (citable) accession number: P0DQD4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 13, 2019 |
Last sequence update: | February 13, 2019 | |
Last modified: | July 3, 2019 | |
This is version 3 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |