UniProtKB - P0DOZ7 (CRFA_CONVC)
Protein
Conorfamide-Vc1
Gene
N/A
Organism
Conus victoriae (Queen Victoria cone)
Status
Functioni
This peptide activates human and mouse sensory neuron-specific G-protein coupled receptors MRGPRX1 (PubMed:30243794). The activity on human receptors has been measured (EC(50)=1.8 µM) (PubMed:30243794). Compared with the agonist chloroquine (anti-malaria drug), it is 200-fold more potent (PubMed:30243794). The peptide also causes an increase in cytosolic calcium in a specific subset of DRG neurons, and, in contrast to other Conus venom peptides, the peptide also affects a large fraction of the non-neuronal cells (PubMed:25464369). In vivo, when intracranially injected into mice, it principally renders mice unable to move, and a very low doses, is causes hyperactivity (PubMed:25464369). It also induces itch sensation, since intradermal cheek injection into humanized transgenic mouse (mouse MRGPRX1 replaced by human MRGPRX1) induces scratching (PubMed:30243794).2 Publications
Miscellaneous
The mature peptide does not contain cysteine residue.Curated
GO - Molecular functioni
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | G-protein coupled receptor impairing toxin, Neurotoxin, Toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Conorfamide-Vc1CuratedShort name: CNF-Vc11 Publication Alternative name(s): Cono-RFamide-Vc11 Publication |
Organismi | Conus victoriae (Queen Victoria cone) |
Taxonomic identifieri | 319920 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Neogastropoda › Conoidea › Conidae › Conus › Cylinder |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 26 – 39 | Missing : Complecte loss of activating potency on MrgprX1 receptor. 1 PublicationAdd BLAST | 14 | |
Mutagenesisi | 26 – 31 | Missing : No change in activity on MrgprX1 receptor. 1 Publication | 6 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
PropeptideiPRO_0000439423 | 20 – 25 | 1 Publication | 6 | |
PeptideiPRO_0000439424 | 26 – 43 | Conorfamide-Vc11 PublicationAdd BLAST | 18 | |
PropeptideiPRO_0000439425 | 45 – 59 | 1 PublicationAdd BLAST | 15 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 43 | Phenylalanine amide1 Publication | 1 |
Keywords - PTMi
Amidation, Cleavage on pair of basic residuesExpressioni
Tissue specificityi
Expressed by the venom duct.1 Publication
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 32 – 39 | Positively charged region crucial for activity against MRGPRX1 receptors1 Publication | 8 |
Sequence similaritiesi
Belongs to the FARP (FMRFamide related peptide) family.Curated
Keywords - Domaini
Signali Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0DOZ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGRGFLLLA LLLLVTVEAT KVEKKHSGFL LAWSGPRNRF VRFGRRDMQS
PLLSERLRL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GAIH01000072 mRNA No translation available. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GAIH01000072 mRNA No translation available. |
3D structure databases
ModBasei | Search... |
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Family and domain databases
ProtoNeti | Search... |
Entry informationi
Entry namei | CRFA_CONVC | |
Accessioni | P0DOZ7Primary (citable) accession number: P0DOZ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2017 |
Last sequence update: | April 12, 2017 | |
Last modified: | February 13, 2019 | |
This is version 8 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families