ADORA3

Functionⁱ

Isoform 2: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase (PubMed:8234299).1 Publication

Isoform 1: Plays a suppressive role in osteosarcoma malignancy by inhibiting NF-kappa-B activity (PubMed:27886186).1 Publication

GO - Molecular functionⁱ

G protein-coupled adenosine receptor activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

activation of adenylate cyclase activity
Source: ProtInc
Traceable author statementⁱ
- 9380026
G protein-coupled receptor signaling pathway Source: Reactome
inflammatory response
Source: ProtInc
Traceable author statementⁱ
- 9164961
negative regulation of cell migration
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 27886186
negative regulation of cell proliferation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 27886186
negative regulation of NF-kappaB transcription factor activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 27886186
regulation of heart contraction
Source: ProtInc
Traceable author statementⁱ
- 9837869
response to wounding
Source: ProtInc
Traceable author statementⁱ
- 9837869
signal transduction
Source: ProtInc
Traceable author statementⁱ
- 8399349
- 9380026

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	G-protein coupled receptor, Receptor, Transducer

Molecular function

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

Reactomeⁱ	R-HSA-417973 Adenosine P1 receptors R-HSA-418594 G alpha (i) signalling events

Reactomeⁱ

R-HSA-417973 Adenosine P1 receptors
R-HSA-418594 G alpha (i) signalling events

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Adenosine receptor A3
Gene namesⁱ	Name:ADORA3Imported
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000282608.1
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:268 ADORA3
MIMⁱ	600445 gene
neXtProtⁱ	NX_P0DMS8

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 14	ExtracellularBy similarityAdd BLAST	14
Transmembraneⁱ	15 – 37	Helical; Name=1By similarityAdd BLAST	23
Topological domainⁱ	38 – 48	CytoplasmicBy similarityAdd BLAST	11
Transmembraneⁱ	49 – 72	Helical; Name=2By similarityAdd BLAST	24
Topological domainⁱ	73 – 84	ExtracellularBy similarityAdd BLAST	12
Transmembraneⁱ	85 – 106	Helical; Name=3By similarityAdd BLAST	22
Topological domainⁱ	107 – 126	CytoplasmicBy similarityAdd BLAST	20
Transmembraneⁱ	127 – 148	Helical; Name=4By similarityAdd BLAST	22
Topological domainⁱ	149 – 177	ExtracellularBy similarityAdd BLAST	29
Transmembraneⁱ	178 – 198	Helical; Name=5By similarityAdd BLAST	21
Topological domainⁱ	199 – 231	CytoplasmicBy similarityAdd BLAST	33
Transmembraneⁱ	232 – 255	Helical; Name=6By similarityAdd BLAST	24
Topological domainⁱ	256 – 261	ExtracellularBy similarity	6
Transmembraneⁱ	262 – 284	Helical; Name=7By similarityAdd BLAST	23
Topological domainⁱ	285 – 318	CytoplasmicBy similarityAdd BLAST	34

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	140
Open Targets More...OpenTargetsⁱ	ENSG00000282608

Chemistry databases

ChEMBLⁱ	CHEMBL256
Drug and drug target database More...DrugBankⁱ	DB00640 Adenosine DB01223 Aminophylline DB05511 CF-101 DB00824 Enprofylline
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	21

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000069010	1 – 318	Adenosine receptor A3Add BLAST		318

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	3	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	4	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	12	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	83 ↔ 166	PROSITE-ProRule annotation
Lipidationⁱ	303	S-palmitoyl cysteineSequence analysis	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEⁱ	P0DMS8

PRIDEⁱ

P0DMS8

PTM databases

iPTMnetⁱ	P0DMS8
PhosphoSitePlusⁱ	P0DMS8

Expressionⁱ

Tissue specificityⁱ

Expressed in the lung and bone. Expressed at lower levels in osteosarcoma tissues (at protein level).1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000282608 Expressed in 129 organ(s), highest expression level in substantia nigra
CleanExⁱ	HS_ADORA3
ExpressionAtlasⁱ	P0DMS8 differential
Genevisibleⁱ	P0DMS8 HS

Interactionⁱ

Chemistry databases

BindingDBⁱ

P0DMS8

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0DMS8
SMRⁱ	P0DMS8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074524
KEGG Orthology (KO) More...KOⁱ	K04268
OMAⁱ	TTQRRIW
PhylomeDBⁱ	P0DMS8

Family and domain databases

InterProⁱ	View protein in InterPro IPR000466 Adeno_A3_rcpt IPR001634 Adenosn_rcpt IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00555 ADENOSINEA3R PR00424 ADENOSINER PR00237 GPCRRHODOPSN
SMARTⁱ	View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 2 (identifier: P0DMS8-1) [UniParc]FASTA Add to basket

Also known as: A3AR i21 Publication, A3AR1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPNNSTALSL ANVTYITMEI FIGLCAIVGN VLVICVVKLN PSLQTTTFYF 
        60         70         80         90        100
IVSLALADIA VGVLVMPLAI VVSLGITIHF YSCLFMTCLL LIFTHASIMS 
       110        120        130        140        150
LLAIAVDRYL RVKLTVRYKR VTTHRRIWLA LGLCWLVSFL VGLTPMFGWN 
       160        170        180        190        200
MKLTSEYHRN VTFLSCQFVS VMRMDYMVYF SFLTWIFIPL VVMCAIYLDI 
       210        220        230        240        250
FYIIRNKLSL NLSNSKETGA FYGREFKTAK SLFLVLFLFA LSWLPLSIIN 
       260        270        280        290        300
CIIYFNGEVP QLVLYMGILL SHANSMMNPI VYAYKIKKFK ETYLLILKAC 
       310 
VVCHPSDSLD TSIEKNSE

Length:318

Mass (Da):36,185

Last modified:April 1, 2015 - v1

Checksum:ⁱ690E67986130FC28

GO

Isoform 1 (identifier: P0DMS9-2) [UniParc]FASTA Add to basket

The sequence of this isoform can be found in the external entry P0DMS9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: The first exon of TMIGD3 isoform 1 (i1) is shared with the first exon of ADORA3 isoform 2 (A3AR i2, commonly known as A3AR), resulting in a fusion protein.1 Publication

Length:347

Mass (Da):38,976

GO

Isoform 3 (identifier: P0DMS9-1) [UniParc]FASTA Add to basket

Also known as: TMIGD3 i3, A3AR i3

The sequence of this isoform can be found in the external entry P0DMS9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Length:266

Mass (Da):30,327

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A0J9YWR0	A0A0J9YWR0_HUMAN		Adenosine receptor A3 Adenosine receptor A3	ADORA3	123	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	7	A → T in AAA16365 (PubMed:8399349).Curated		1
Sequence conflictⁱ	175	D → V in AAG35155 (PubMed:11214319).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_035755	105	A → T in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs746154553Ensembl.	1
Natural variantⁱVAR_049366	248	I → L1 PublicationCorresponds to variant dbSNP:rs35511654Ensembl.	1
Natural variantⁱVAR_049367	266	M → K. Corresponds to variant dbSNP:rs2800889Ensembl.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L20463 mRNA Translation: AAA16365.1 L22607 mRNA Translation: AAA35949.1 X76981 mRNA Translation: CAA54288.1 L77730, L77729 Genomic DNA Translation: AAB02790.1 AY136749 mRNA Translation: AAN01275.1 AL390195 Genomic DNA No translation available. BC029831 mRNA Translation: AAH29831.1 AY011231 Genomic DNA Translation: AAG35155.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS839.1 [P0DMS8-1]
PIRⁱ	S38511
NCBI Reference Sequences More...RefSeqⁱ	NP_000668.1, NM_000677.3 [P0DMS8-1]
UniGeneⁱ	Hs.281342

Genome annotation databases

Ensemblⁱ	ENST00000241356; ENSP00000241356; ENSG00000282608 [P0DMS8-1]
GeneIDⁱ	140
KEGGⁱ	hsa:140

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P0DMS8	Adenosine receptor A3		PANPA		318	UniRef100_P0DMS8
Adenosine receptor A3 (Fragment)		HUMAN		317
ADORA3 (Fragment)		PANTR		107
UPI000166C3C3		HUMAN		173

Protein	Similar proteins		Species	Score	Length	Source
P0DMS8	Adenosine receptor A3		PANPA		318	UniRef90_P0DMS8
Adenosine receptor A3 (Fragment)		HUMAN		317
ADORA3 (Fragment)		PANTR		107
Adenosine receptor A3		CALJA		318
Adenosine receptor A3		SAIBB		318
+31
P0DMS9-2	Uncharacterized protein		MACMU		347	UniRef90_P0DMS9-2
Macaca fascicularis brain cDNA clone: QorA-14524, similar to human AD026 protein (AD026), mRNA, RefSeq: NM_020683.5		MACFA		347
Uncharacterized protein		COLAP		347
TMIGD3 isoform 3		PONAB		347
Uncharacterized protein		PANTR		347
+31

Protein	Similar proteins		Species	Score	Length	Source
P0DMS8	Adenosine receptor A3		SHEEP		317	UniRef50_P0DMS8
Adenosine receptor A3		RABIT		319
Adenosine receptor A3		BOVIN		317
Adenosine receptor A3		CANLF		314
Adenosine receptor A3		PANPA		318
+353
P0DMS9-2	Uncharacterized protein		PANPA		347	UniRef50_P0DMS9-2
Uncharacterized protein		COLAP		347
TMIGD3 isoform 3		PANTR		347
Uncharacterized protein		MACMU		347
Macaca fascicularis brain cDNA clone: QorA-14524, similar to human AD026 protein (AD026), mRNA, RefSeq: NM_020683.5		MACFA		347
+68

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L20463 mRNA Translation: AAA16365.1 L22607 mRNA Translation: AAA35949.1 X76981 mRNA Translation: CAA54288.1 L77730, L77729 Genomic DNA Translation: AAB02790.1 AY136749 mRNA Translation: AAN01275.1 AL390195 Genomic DNA No translation available. BC029831 mRNA Translation: AAH29831.1 AY011231 Genomic DNA Translation: AAG35155.1
CCDSⁱ	CCDS839.1 [P0DMS8-1]
PIRⁱ	S38511
RefSeqⁱ	NP_000668.1, NM_000677.3 [P0DMS8-1]
UniGeneⁱ	Hs.281342

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1OEA	model	-	A	1-298	[»]
	1R7N	model	-	A	1-318	[»]
ProteinModelPortalⁱ	P0DMS8
SMRⁱ	P0DMS8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Chemistry databases

BindingDBⁱ	P0DMS8
ChEMBLⁱ	CHEMBL256
DrugBankⁱ	DB00640 Adenosine DB01223 Aminophylline DB05511 CF-101 DB00824 Enprofylline
GuidetoPHARMACOLOGYⁱ	21

Protein family/group databases

GPCRDBⁱ	Search...

GPCRDBⁱ

Search...

PTM databases

iPTMnetⁱ	P0DMS8
PhosphoSitePlusⁱ	P0DMS8

Proteomic databases

PRIDEⁱ	P0DMS8

PRIDEⁱ

P0DMS8

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000241356; ENSP00000241356; ENSG00000282608 [P0DMS8-1]
GeneIDⁱ	140
KEGGⁱ	hsa:140

Organism-specific databases

CTDⁱ	140
DisGeNETⁱ	140
EuPathDBⁱ	HostDB:ENSG00000282608.1
GeneCardsⁱ	ADORA3
HGNCⁱ	HGNC:268 ADORA3
MIMⁱ	600445 gene
neXtProtⁱ	NX_P0DMS8
OpenTargetsⁱ	ENSG00000282608
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

GeneTreeⁱ	ENSGT00550000074524
KOⁱ	K04268
OMAⁱ	TTQRRIW
PhylomeDBⁱ	P0DMS8

Enzyme and pathway databases

Reactomeⁱ	R-HSA-417973 Adenosine P1 receptors R-HSA-418594 G alpha (i) signalling events

Reactomeⁱ

R-HSA-417973 Adenosine P1 receptors
R-HSA-418594 G alpha (i) signalling events

Miscellaneous databases

ChiTaRSⁱ	ADORA3 human
GenomeRNAiⁱ	140
Protein Ontology More...PROⁱ	PR:P0DMS8
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000282608 Expressed in 129 organ(s), highest expression level in substantia nigra
CleanExⁱ	HS_ADORA3
ExpressionAtlasⁱ	P0DMS8 differential
Genevisibleⁱ	P0DMS8 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR000466 Adeno_A3_rcpt IPR001634 Adenosn_rcpt IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM
Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00555 ADENOSINEA3R PR00424 ADENOSINER PR00237 GPCRRHODOPSN
SMARTⁱ	View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AA3R_HUMAN
Accessionⁱ	P0DMS8Primary (citable) accession number: P0DMS8 Secondary accession number(s): A2A3P4 , P33765, Q6UWU0, Q9BYZ1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 2015
	Last sequence update:	April 1, 2015
	Last modified:	November 7, 2018
	This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries

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UniProtKB - P0DMS8 (AA3R_HUMAN)

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Adenosine receptor A3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

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Plasma membrane

Plasma Membrane

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

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Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

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Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

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<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ