UniProtKB - P0DMC7 (RCSB_ECOLI)
Protein
Transcriptional regulatory protein RcsB
Gene
rcsB
Organism
Escherichia coli (strain K12)
Status
Functioni
Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.UniRule annotation7 Publications
Miscellaneous
There is a close linkage between the Rcs and PhoQ/P signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium).1 Publication
Activity regulationi
Activity is modulated by phosphorylation and interaction with other transcriptional regulators. Probably up-regulated by YmgA/AriR, and possibly down-regulated by YcgZ, all 3 are connector proteins providing additional signal input into the signaling system.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 168 – 187 | H-T-H motifUniRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-UniRule
- DNA-binding transcription activator activity Source: EcoCyc
- DNA-binding transcription repressor activity Source: EcoCyc
- identical protein binding Source: IntAct
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: EcoCyc
- phosphorelay signal transduction system Source: UniProtKB-UniRule
- positive regulation of cell projection organization Source: CACAO
- positive regulation of transcription, DNA-templated Source: EcoCyc
- response to antibiotic Source: CACAO
- single-species biofilm formation on inanimate substrate Source: CACAO
- transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Capsule biogenesis/degradation, Transcription, Transcription regulation, Two-component regulatory system |
Enzyme and pathway databases
BioCyci | EcoCyc:RCSB-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional regulatory protein RcsBUniRule annotationAlternative name(s): Capsular synthesis regulator component B |
Gene namesi | Name:rcsBUniRule annotation Ordered Locus Names:b2217, JW2205 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 56 | D → E: Increases heterodimer formation with RcsA. Does not affect heterodimer formation with BglJ. 1 Publication | 1 | |
Mutagenesisi | 56 | D → N: Decreases heterodimer formation with RcsA. Does not affect heterodimer formation with BglJ. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081209 | 1 – 216 | Transcriptional regulatory protein RcsBAdd BLAST | 216 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 56 | 4-aspartylphosphateUniRule annotation | 1 |
Post-translational modificationi
Phosphorylated and activated by RcsD.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0DMC7 |
PaxDbi | P0DMC7 |
PRIDEi | P0DMC7 |
Expressioni
Inductioni
Expression is dependent on the alternate sigma factor, RpoN (PubMed:2404948). Repressed by hydroxyurea.2 Publications
Interactioni
Subunit structurei
Interacts with RcsD and RcsA. Homodimer. Can form an heterodimer with the coactivator RcsA, which binds to the RcsAB boxes to promote transcription of RcsA-dependent genes. Can also form a heterodimer with GadE to bind to the GAD box, and with BglJ to bind to the bgl promoter.
UniRule annotation6 PublicationsBinary interactionsi
Hide detailsP0DMC7
With | #Exp. | IntAct |
---|---|---|
bglJ [P39404] | 4 | EBI-369670,EBI-551429 |
dctR [P37195] | 3 | EBI-369670,EBI-562540 |
itself | 3 | EBI-369670,EBI-369670 |
rcsD [P39838] | 5 | EBI-369670,EBI-556803 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4260879, 5 interactors 851761, 4 interactors |
ComplexPortali | CPX-5762, rcsAB DNA-binding transcription factor complex CPX-5763, bglJ-rcsB DNA-binding transcription factor complex CPX-5764, matA-rcsB DNA-binding transcription factor complex CPX-5765, rcsB DNA-binding transcription factor homodimer CPX-5781, gadE-rcsB DNA-binding transcription factor complex |
IntActi | P0DMC7, 43 interactors |
STRINGi | 511145.b2217 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0DMC7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 124 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 120 | |
Domaini | 144 – 209 | HTH luxR-typeUniRule annotationAdd BLAST | 66 |
Sequence similaritiesi
Belongs to the RcsB family.UniRule annotation
Phylogenomic databases
eggNOGi | COG2197, Bacteria |
HOGENOMi | CLU_000445_90_1_6 |
PhylomeDBi | P0DMC7 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00981, RcsB, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR030864, RcsB IPR016032, Sig_transdc_resp-reg_C-effctor IPR001789, Sig_transdc_resp-reg_receiver IPR000792, Tscrpt_reg_LuxR_C IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00196, GerE, 1 hit PF00072, Response_reg, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS00622, HTH_LUXR_1, 1 hit PS50043, HTH_LUXR_2, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
P0DMC7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNNMNVIIAD DHPIVLFGIR KSLEQIEWVN VVGEFEDSTA LINNLPKLDA
60 70 80 90 100
HVLITDLSMP GDKYGDGITL IKYIKRHFPS LSIIVLTMNN NPAILSAVLD
110 120 130 140 150
LDIEGIVLKQ GAPTDLPKAL AALQKGKKFT PESVSRLLEK ISAGGYGDKR
160 170 180 190 200
LSPKESEVLR LFAEGFLVTE IAKKLNRSIK TISSQKKSAM MKLGVENDIA
210
LLNYLSSVTL SPADKD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28242 Genomic DNA Translation: AAA24504.1 U00096 Genomic DNA Translation: AAC75277.1 AP009048 Genomic DNA Translation: BAA16000.1 S37760 Genomic DNA Translation: AAB22290.1 |
PIRi | JV0068, BVECCB |
RefSeqi | NP_416721.1, NC_000913.3 WP_001061917.1, NZ_STEB01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC75277; AAC75277; b2217 BAA16000; BAA16000; BAA16000 |
GeneIDi | 58463116 947441 |
KEGGi | ecj:JW2205 eco:b2217 |
PATRICi | fig|1411691.4.peg.18 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28242 Genomic DNA Translation: AAA24504.1 U00096 Genomic DNA Translation: AAC75277.1 AP009048 Genomic DNA Translation: BAA16000.1 S37760 Genomic DNA Translation: AAB22290.1 |
PIRi | JV0068, BVECCB |
RefSeqi | NP_416721.1, NC_000913.3 WP_001061917.1, NZ_STEB01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5I4C | X-ray | 2.00 | A | 1-147 | [»] | |
5VXN | X-ray | 3.38 | A/B/C/D | 1-216 | [»] | |
5W43 | X-ray | 3.15 | A/B | 1-216 | [»] | |
SMRi | P0DMC7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260879, 5 interactors 851761, 4 interactors |
ComplexPortali | CPX-5762, rcsAB DNA-binding transcription factor complex CPX-5763, bglJ-rcsB DNA-binding transcription factor complex CPX-5764, matA-rcsB DNA-binding transcription factor complex CPX-5765, rcsB DNA-binding transcription factor homodimer CPX-5781, gadE-rcsB DNA-binding transcription factor complex |
IntActi | P0DMC7, 43 interactors |
STRINGi | 511145.b2217 |
Proteomic databases
jPOSTi | P0DMC7 |
PaxDbi | P0DMC7 |
PRIDEi | P0DMC7 |
Genome annotation databases
EnsemblBacteriai | AAC75277; AAC75277; b2217 BAA16000; BAA16000; BAA16000 |
GeneIDi | 58463116 947441 |
KEGGi | ecj:JW2205 eco:b2217 |
PATRICi | fig|1411691.4.peg.18 |
Organism-specific databases
EchoBASEi | EB0814 |
Phylogenomic databases
eggNOGi | COG2197, Bacteria |
HOGENOMi | CLU_000445_90_1_6 |
PhylomeDBi | P0DMC7 |
Enzyme and pathway databases
BioCyci | EcoCyc:RCSB-MONOMER |
Miscellaneous databases
PROi | PR:P0DMC7 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00981, RcsB, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR030864, RcsB IPR016032, Sig_transdc_resp-reg_C-effctor IPR001789, Sig_transdc_resp-reg_receiver IPR000792, Tscrpt_reg_LuxR_C IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00196, GerE, 1 hit PF00072, Response_reg, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS00622, HTH_LUXR_1, 1 hit PS50043, HTH_LUXR_2, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RCSB_ECOLI | |
Accessioni | P0DMC7Primary (citable) accession number: P0DMC7 Secondary accession number(s): P14374, P69407 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 19, 2014 |
Last sequence update: | February 19, 2014 | |
Last modified: | April 7, 2021 | |
This is version 58 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families