UniProtKB - P0DM78 (PHOP_SALTY)
Protein
Virulence transcriptional regulatory protein PhoP
Gene
phoP
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Functioni
Member of the two-component regulatory system PhoP/PhoQ which regulates the expression of genes involved in virulence, adaptation to acidic and low Mg2+ environments and resistance to host defense antimicrobial peptides. Essential for intramacrophage survival of S.typhimurium. In low periplasmic Mg2+, PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg2+, PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Essential for transcription of spiC inside macrophages by controlling the expression of the two-component regulatory system SsrB/SpiR (SsrA) and Pir at transcriptional and post-transcriptional levels respectively. Promotes expression of the two-component regulatory system PmrA/PmrB via activation of pmrD gene. Is required to attenuate bacterial growth within fibroblast cells and to enhance bacterial resistance to bile in intestinal cells. Negatively regulates prgH, which is required for invasion of epithelial cells. PhoP uses multiple mechanisms to promote transcription and activates promoters for PAG at low (µM range) Mg2+ concentrations (Probable). Involved in acid tolerance.Curated2 Publications
Miscellaneous
PhoP/PhoQ-signaling cascade, which activates virulence membrane proteins (PagC, PagO, PagD, PagK, PgtE and PhoN), is induced by cationic antimicrobial peptides (CAMP) (polymyxin, alpha-helical peptide C18G and sheet peptide protegrin-1) at sublethal concentrations.Curated
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 125 – 223 | OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST | 99 |
GO - Molecular functioni
- DNA-binding transcription activator activity Source: GO_Central
- phosphorelay response regulator activity Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- pathogenesis Source: UniProtKB-KW
- regulation of growth Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Growth regulation, Transcription, Transcription regulation, Two-component regulatory system, Virulence |
Names & Taxonomyi
Protein namesi | Recommended name: Virulence transcriptional regulatory protein PhoPAlternative name(s): Acid shock protein 29 Short name: ASP29 |
Gene namesi | Name:phoP Ordered Locus Names:STM1231 |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: GO_Central
Other locations
- protein-DNA complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
10,000-fold reduction in virulence in male BALB/c mice (for strain ATCC 14028). Decreased tolerance to acid stress.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | D → A: Expression at concentrations above 30 uM retains a protein-protein interaction and PhoQ-independent induction of PAG. Yields insoluble purified protein above 7 uM. 1 Publication | 1 |
Miscellaneous databases
PHI-basei | PHI:2674 PHI:6591 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081201 | 1 – 224 | Virulence transcriptional regulatory protein PhoPAdd BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 52 | 4-aspartylphosphatePROSITE-ProRule annotation | 1 |
Post-translational modificationi
Phosphorylated and dephosphorylated by PhoQ.Curated
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P0DM78 |
Expressioni
Inductioni
The phoP/phoQ operon is positively autoregulated by both PhoP and PhoQ in a Mg2+-dependent manner. Repressed by RcsB via sigma factor RpoS.
Gene expression databases
CollecTFi | EXPREG_000008e0 |
Interactioni
Subunit structurei
Monomer in the inactive, unphosphorylated state and dimer in the active, phosphorylated state.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 117 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 115 |
Phylogenomic databases
HOGENOMi | CLU_000445_30_1_6 |
OMAi | NKRGFGY |
PhylomeDBi | P0DM78 |
Family and domain databases
CDDi | cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001867, OmpR/PhoB-type_DNA-bd IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR48111, PTHR48111, 1 hit |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
P0DM78-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMRVLVVEDN ALLRHHLKVQ LQDSGHQVDA AEDAREADYY LNEHLPDIAI
60 70 80 90 100
VDLGLPDEDG LSLIRRWRSS DVSLPVLVLT AREGWQDKVE VLSSGADDYV
110 120 130 140 150
TKPFHIEEVM ARMQALMRRN SGLASQVINI PPFQVDLSRR ELSVNEEVIK
160 170 180 190 200
LTAFEYTIME TLIRNNGKVV SKDSLMLQLY PDAELRESHT IDVLMGRLRK
210 220
KIQAQYPHDV ITTVRGQGYL FELR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24424 Genomic DNA Translation: AAA27188.1 M25241 Genomic DNA Translation: AAA27187.1 AE006468 Genomic DNA Translation: AAL20160.1 |
PIRi | A32932, RGEBFT |
RefSeqi | NP_460201.1, NC_003197.2 WP_000986522.1, NC_003197.2 |
Genome annotation databases
EnsemblBacteriai | AAL20160; AAL20160; STM1231 |
GeneIDi | 1252749 |
KEGGi | stm:STM1231 |
PATRICi | fig|99287.12.peg.1302 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24424 Genomic DNA Translation: AAA27188.1 M25241 Genomic DNA Translation: AAA27187.1 AE006468 Genomic DNA Translation: AAL20160.1 |
PIRi | A32932, RGEBFT |
RefSeqi | NP_460201.1, NC_003197.2 WP_000986522.1, NC_003197.2 |
3D structure databases
SMRi | P0DM78 |
ModBasei | Search... |
Proteomic databases
PaxDbi | P0DM78 |
Genome annotation databases
EnsemblBacteriai | AAL20160; AAL20160; STM1231 |
GeneIDi | 1252749 |
KEGGi | stm:STM1231 |
PATRICi | fig|99287.12.peg.1302 |
Phylogenomic databases
HOGENOMi | CLU_000445_30_1_6 |
OMAi | NKRGFGY |
PhylomeDBi | P0DM78 |
Miscellaneous databases
PHI-basei | PHI:2674 PHI:6591 |
Gene expression databases
CollecTFi | EXPREG_000008e0 |
Family and domain databases
CDDi | cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001867, OmpR/PhoB-type_DNA-bd IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR48111, PTHR48111, 1 hit |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHOP_SALTY | |
Accessioni | P0DM78Primary (citable) accession number: P0DM78 Secondary accession number(s): P14146 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2013 |
Last sequence update: | November 13, 2013 | |
Last modified: | April 7, 2021 | |
This is version 52 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |