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Entry version 37 (25 May 2022)
Sequence version 1 (03 Sep 2014)
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Protein

High-affinity nitrate transporter 2.2

Gene

NRT2.2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in nitrate transport, but does not seem to be able to mediate transport by its own. Acts as a dual component transporter with NAR2.1. Imports nitrate with high affinity when expressed with NAR2.1 in a heterologous system (Xenopus oocytes).

3 Publications

Miscellaneous

Plants over-expressing NRT2.2 have accelerated growth under low nitrate conditions (Ref. 6).

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=34 µM for nitrate1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processNitrate assimilation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
High-affinity nitrate transporter 2.2
Short name:
OsNRT2.2
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NRT2.2Curated
Ordered Locus Names:Os02g0112600Imported, LOC_Os02g02190Curated
ORF Names:OJ1399_H05.7Imported, OsJ_05080Imported, P0501G04.36Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiOryza sativa subsp. japonica (Rice)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri39947 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000007752 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2
  • UP000059680 Componenti: Chromosome 2
  • UP000000763 Componenti: Chromosome 2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
Transmembranei101 – 122HelicalSequence analysisAdd BLAST22
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Transmembranei227 – 247HelicalSequence analysisAdd BLAST21
Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
Transmembranei319 – 339HelicalSequence analysisAdd BLAST21
Transmembranei351 – 371HelicalSequence analysisAdd BLAST21
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Transmembranei411 – 431HelicalSequence analysisAdd BLAST21
Transmembranei442 – 462HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004300051 – 533High-affinity nitrate transporter 2.2Add BLAST533

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in primary and lateral roots, root-shoot junction zone, vascular tissues of adventitious root primordia, leaves, embryo and coleoptiles of germinating seeds, husks and anthers.1 Publication

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Circadian-regulation with a peak in the middle of the morning and at the end of the light period. Induced by nitrate and sucrose in roots. Down-regulated by ammonium, glutamine, asparagine and aspartate in roots.3 Publications

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
P0DKH0, baseline and differential

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterotetramer composed of two NRT2.2 and two NAR2.1 (By similarity).

Interacts with NAR2.1.

By similarity1 Publication

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
39947.P0DKH0

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

AlphaFold Protein Structure Database

More...
AlphaFoldDBi
P0DKH0

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P0DKH0

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_024204_0_0_1

Database of Orthologous Groups

More...
OrthoDBi
542533at2759

Family and domain databases

Conserved Domains Database

More...
CDDi
cd17341, MFS_NRT2_like, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.1250.20, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011701, MFS
IPR036259, MFS_trans_sf
IPR044772, NO3_transporter

The PANTHER Classification System

More...
PANTHERi
PTHR23515, PTHR23515, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07690, MFS_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103473, SSF103473, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0DKH0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDSSTVGAPG SSLHGVTGRE PAFAFSTEVG GEDAAAASKF DLPVDSEHKA
60 70 80 90 100
KTIRLLSFAN PHMRTFHLSW ISFFSCFVST FAAAPLVPII RDNLNLTKAD
110 120 130 140 150
IGNAGVASVS GSIFSRLAMG AICDMLGPRY GCAFLIMLAA PTVFCMSLID
160 170 180 190 200
SAAGYIAVRF LIGFSLATFV SCQYWMSTMF NSKIIGLVNG LAAGWGNMGG
210 220 230 240 250
GATQLIMPLV YDVIRKCGAT PFTAWRLAYF VPGTLHVVMG VLVLTLGQDL
260 270 280 290 300
PDGNLRSLQK KGDVNRDSFS RVLWYAVTNY RTWIFVLLYG YSMGVELTTD
310 320 330 340 350
NVIAEYFYDR FDLDLRVAGI IAASFGMANI VARPTGGLLS DLGARYFGMR
360 370 380 390 400
ARLWNIWILQ TAGGAFCLLL GRASTLPTSV VCMVLFSFCA QAACGAIFGV
410 420 430 440 450
IPFVSRRSLG IISGMTGAGG NFGAGLTQLL FFTSSRYSTG TGLEYMGIMI
460 470 480 490 500
MACTLPVVLV HFPQWGSMFL PPNAGAEEEH YYGSEWSEQE KSKGLHGASL
510 520 530
KFAENSRSER GRRNVINAAA AAATPPNNSP EHA
Length:533
Mass (Da):57,230
Last modified:September 3, 2014 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i29665CAC0E24750E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AP004090 Genomic DNA Translation: BAD07629.1
AP004752 Genomic DNA Translation: BAD07853.1
AP008208 Genomic DNA Translation: BAF07573.1
AP014958 Genomic DNA Translation: BAS76626.1
CM000139 Genomic DNA Translation: EAZ21473.1
AK109733 mRNA Translation: BAG98877.1

NCBI Reference Sequences

More...
RefSeqi
XP_015623596.1, XM_015768110.1
XP_015623638.1, XM_015768152.1

Genome annotation databases

Ensembl plant genome annotation project

More...
EnsemblPlantsi
Os02t0112100-01; Os02t0112100-01; Os02g0112100
Os02t0112600-01; Os02t0112600-01; Os02g0112600

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
4328051
4328052

Gramene; a comparative resource for plants

More...
Gramenei
Os02t0112100-01; Os02t0112100-01; Os02g0112100
Os02t0112600-01; Os02t0112600-01; Os02g0112600

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
osa:4328051
osa:4328052

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004090 Genomic DNA Translation: BAD07629.1
AP004752 Genomic DNA Translation: BAD07853.1
AP008208 Genomic DNA Translation: BAF07573.1
AP014958 Genomic DNA Translation: BAS76626.1
CM000139 Genomic DNA Translation: EAZ21473.1
AK109733 mRNA Translation: BAG98877.1
RefSeqiXP_015623596.1, XM_015768110.1
XP_015623638.1, XM_015768152.1

3D structure databases

AlphaFoldDBiP0DKH0
SMRiP0DKH0
ModBaseiSearch...

Protein-protein interaction databases

STRINGi39947.P0DKH0

Genome annotation databases

EnsemblPlantsiOs02t0112100-01; Os02t0112100-01; Os02g0112100
Os02t0112600-01; Os02t0112600-01; Os02g0112600
GeneIDi4328051
4328052
GrameneiOs02t0112100-01; Os02t0112100-01; Os02g0112100
Os02t0112600-01; Os02t0112600-01; Os02g0112600
KEGGiosa:4328051
osa:4328052

Phylogenomic databases

HOGENOMiCLU_024204_0_0_1
OrthoDBi542533at2759

Gene expression databases

ExpressionAtlasiP0DKH0, baseline and differential

Family and domain databases

CDDicd17341, MFS_NRT2_like, 1 hit
Gene3Di1.20.1250.20, 2 hits
InterProiView protein in InterPro
IPR011701, MFS
IPR036259, MFS_trans_sf
IPR044772, NO3_transporter
PANTHERiPTHR23515, PTHR23515, 1 hit
PfamiView protein in Pfam
PF07690, MFS_1, 1 hit
SUPFAMiSSF103473, SSF103473, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNRT22_ORYSJ
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0DKH0
Secondary accession number(s): A0A0N7KEJ9, Q6ZH34
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 3, 2014
Last sequence update: September 3, 2014
Last modified: May 25, 2022
This is version 37 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families
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