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Entry version 13 (02 Jun 2021)
Sequence version 1 (09 Dec 2015)
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Protein

Replicase polyprotein 1TF

Gene
N/A
Organism
Porcine reproductive and respiratory syndrome virus (strain Lelystad) (PRRSV)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Inhibits host IFN-beta production. Plays a role in the degradation of the host transcriptional activator CREBBP protein. The degradation of host CREBBP which is a key component of the IFN enhanceosome is likely responsible for the inhibition of interferon mediated by Nsp1-alpha. Participates also in the inhibition of host NF-kappa-B activation.

By similarity1 Publication

Plays a role in the inhibition of the interferon-activated JAK/STAT signal transduction by mediating the ubiquitination and subsequent proteasomal degradation of host KPNA1.

By similarity

Plays a role in viral replication.

1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei76For Nsp1-alpha papain-like cysteine proteinase activityPROSITE-ProRule annotationBy similarity1
Active sitei146For Nsp1-alpha papain-like cysteine proteinase activityPROSITE-ProRule annotationBy similarity1
Active sitei276For Nsp1-beta papain-like cysteine proteinase activityPROSITE-ProRule annotationBy similarity1
Active sitei345For Nsp1-beta papain-like cysteine proteinase activityPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri8 – 28C4-type; atypicalBy similarityAdd BLAST21

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandMetal-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Replicase polyprotein 1TF
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 4 chains:
Nsp1 (EC:3.4.22.-)
Nsp1-alpha papain-like cysteine proteinase (EC:3.4.22.-)
Alternative name(s):
PCP1-alpha
Nsp1-beta papain-like cysteine proteinase (EC:3.4.22.-)
Alternative name(s):
PCP1-beta
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPorcine reproductive and respiratory syndrome virus (strain Lelystad) (PRRSV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri11049 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaOrthornaviraePisuviricotaPisoniviricetesNidoviralesArnidovirineaeArteriviridaeVariarterivirinaeBetaarterivirusEurpobartevirusBetaarterivirus suid 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiSus scrofa (Pig) [TaxID: 9823]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006687 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

  • Host nucleus 1 Publication
  • Host cytoplasm 1 Publication
  • Host nucleus 1 Publication
  • Host cytoplasm 1 Publication
  • Host nucleus 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei1136 – 1156HelicalSequence analysisAdd BLAST21
Transmembranei1170 – 1190HelicalSequence analysisAdd BLAST21
Transmembranei1211 – 1231HelicalSequence analysisAdd BLAST21
Transmembranei1250 – 1270HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Host nucleus, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004348741 – 1285Replicase polyprotein 1TFAdd BLAST1285
ChainiPRO_00004348751 – 384Nsp1By similarityAdd BLAST384
ChainiPRO_00004348761 – 180Nsp1-alpha papain-like cysteine proteinaseSequence analysisAdd BLAST180
ChainiPRO_0000434877181 – 385Nsp1-beta papain-like cysteine proteinaseSequence analysisAdd BLAST205
ChainiPRO_0000434878386 – 1285Nsp2TFAdd BLAST900

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei180 – 181Cleavage; by autolysisSequence analysis2
Sitei385 – 386Cleavage; by autolysisBy similarity2

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P0DJZ9

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini69 – 180Peptidase C31PROSITE-ProRule annotationAdd BLAST112
Domaini269 – 385Peptidase C32PROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni69 – 182PCP1-alphaBy similarityAdd BLAST114
Regioni269 – 384PCP1-betaBy similarityAdd BLAST116
Regioni752 – 797DisorderedSequence analysisAdd BLAST46
Regioni1050 – 1088DisorderedSequence analysisAdd BLAST39

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi773 – 788Polar residuesSequence analysisAdd BLAST16

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri8 – 28C4-type; atypicalBy similarityAdd BLAST21

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.90.70.60, 1 hit
3.90.70.70, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR008743, Arterivirus_Nsp2_C33
IPR008741, AV_PCPalpha
IPR038155, AV_PCPalpha_sf
IPR025773, AV_PCPbeta
IPR038154, AV_PCPbeta_sf
IPR032855, NSP2-B_epitope
IPR032841, NSP2_assoc

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF14757, NSP2-B_epitope, 1 hit
PF14758, NSP2_assoc, 1 hit
PF05410, Peptidase_C31, 1 hit
PF05411, Peptidase_C32, 1 hit
PF05412, Peptidase_C33, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51538, AV_CP, 1 hit
PS51539, AV_PCP_ALPHA, 1 hit
PS51540, AV_PCP_BETA, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by ribosomal frameshifting. AlignAdd to basket
Isoform Replicase polyprotein 1TF (identifier: P0DJZ9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGTFSRCMC TPAARVFWNA GQVFCTRCLS ARSLLSPELQ DTDLGAVGLF
60 70 80 90 100
YKPRDKLHWK VPIGIPQVEC TPSGCCWLSA VFPLARMTSG NHNFLQRLVK
110 120 130 140 150
VADVLYRDGC LAPRHLRELQ VYERGCNWYP ITGPVPGMGL FANSMHVSDQ
160 170 180 190 200
PFPGATHVLT NSPLPQQACR QPFCPFEEAH SSVYRWKKFV VFTDSSLNGR
210 220 230 240 250
SRMMWTPESD DSAALEVLPP ELERQVEILI RSFPAHHPVD LADWELTESP
260 270 280 290 300
ENGFSFNTSH SCGHLVQNPD VFDGKCWLSC FLGQSVEVRC HEEHLADAFG
310 320 330 340 350
YQTKWGVHGK YLQRRLQVRG IRAVVDPDGP IHVEALSCPQ SWIRHLTLDD
360 370 380 390 400
DVTPGFVRLT SLRIVPNTEP TTSRIFRFGA HKWYGAAGKR ARAKRAAKSE
410 420 430 440 450
KDSAPTPKVA LPVPTCGITT YSPPTDGSCG WHVLAAIMNR MINGDFTSPL
460 470 480 490 500
TQYNRPEDDW ASDYDLVQAI QCLRLPATVV RNRACPNAKY LIKLNGVHWE
510 520 530 540 550
VEVRSGMAPR SLSRECVVGV CSEGCVAPPY PADGLPKRAL EALASAYRLP
560 570 580 590 600
SDCVSSGIAD FLANPPPQEF WTLDKMLTSP SPERSGFSSL YKLLLEVVPQ
610 620 630 640 650
KCGATEGAFI YAVERMLKDC PSSKQAMALL AKIKVPSSKA PSVSLDECFP
660 670 680 690 700
TDVLADFEPA SQERPQSSGA AVVLCSPDAK EFEEAAPEEV QESGHKAVHS
710 720 730 740 750
ALLAEGPNNE QVQVVAGEQL KLGGCGLAVG NAHEGALVSA GLINLVGGNL
760 770 780 790 800
SPSDPMKENM LNSREDEPLD LSQPAPASTT TLVREQTPDN PGSDAGALPV
810 820 830 840 850
TVREFVPTGP ILCHVEHCGT ESGDSSSPLD LSDAQTLDQP LNLSLAAWPV
860 870 880 890 900
RATASDPGWV HGRREPVFVK PRNAFSDGDS ALQFGELSES SSVIEFDRTK
910 920 930 940 950
DAPVVDAPVD LTTSNEALSV VDPFEFAELK RPRFSAQALI DRGGPLADVH
960 970 980 990 1000
AKIKNRVYEQ CLQACEPGSR ATPATREWLD KMWDRVDMKT WRCTSQFQAG
1010 1020 1030 1040 1050
RILASLKFLP DMIQDTPPPV PRKNRASDNA GLKQLVAQWD RKLSVTPPPK
1060 1070 1080 1090 1100
PVGPVLDQIV PPPTDIQQED VTPSDGPPHA PDFPSRVSTG GSWKGLMLSG
1110 1120 1130 1140 1150
TRLAGSISQR LMTWVFLKFS PTSQLLCSHF SRRGALWLQV IGCLQVSFYL
1160 1170 1180 1190 1200
LSCSVVLTRY SDAFPYWVSF LVLCGVFVWV FLVLGWLLLY FYSRLHPTQS
1210 1220 1230 1240 1250
VLLVTTIRRS VMLSFWLLSS ANFGNLCAAL WSAPQASYVS FLASYSVGHV
1260 1270 1280
ISGMFSYVYA CLQIWPFLLF MWCPRGVVTS VGESV
Note: Produced by a -2 ribosomal frameshift.
Length:1,285
Mass (Da):141,133
Last modified:December 9, 2015 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i548F4A21A77E6BE1
GO
Isoform Replicase polyprotein 1ab (identifier: Q04561-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry Q04561.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:3,855
Mass (Da):421,332
GO
Isoform Replicase polyprotein 1a (identifier: Q04561-2) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein
The sequence of this isoform can be found in the external entry Q04561.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:2,396
Mass (Da):260,039
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M96262 Genomic RNA No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96262 Genomic RNA No translation available.

3D structure databases

SMRiP0DJZ9
ModBaseiSearch...

Family and domain databases

Gene3Di3.90.70.60, 1 hit
3.90.70.70, 1 hit
InterProiView protein in InterPro
IPR008743, Arterivirus_Nsp2_C33
IPR008741, AV_PCPalpha
IPR038155, AV_PCPalpha_sf
IPR025773, AV_PCPbeta
IPR038154, AV_PCPbeta_sf
IPR032855, NSP2-B_epitope
IPR032841, NSP2_assoc
PfamiView protein in Pfam
PF14757, NSP2-B_epitope, 1 hit
PF14758, NSP2_assoc, 1 hit
PF05410, Peptidase_C31, 1 hit
PF05411, Peptidase_C32, 1 hit
PF05412, Peptidase_C33, 1 hit
PROSITEiView protein in PROSITE
PS51538, AV_CP, 1 hit
PS51539, AV_PCP_ALPHA, 1 hit
PS51540, AV_PCP_BETA, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRPOTF_PRRSL
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0DJZ9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 9, 2015
Last sequence update: December 9, 2015
Last modified: June 2, 2021
This is version 13 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome
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