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Protein

Carboxyethyl-arginine beta-lactam-synthase

Gene

bls

Organism
Streptomyces clavuligerus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-N2-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Pathwayi: clavulanate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes clavulanate from D-glyceraldehyde 3-phosphate and L-arginine.
Proteins known to be involved in the 8 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Carboxyethyl-arginine beta-lactam-synthase (bls)
  3. no protein annotated in this organism
  4. Proclavaminate amidinohydrolase (pah)
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. no protein annotated in this organism
  8. no protein annotated in this organism
This subpathway is part of the pathway clavulanate biosynthesis, which is itself part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes clavulanate from D-glyceraldehyde 3-phosphate and L-arginine, the pathway clavulanate biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi253Magnesium1
Metal bindingi351Magnesium1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13483
UniPathwayi
UPA00112;UER00243

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxyethyl-arginine beta-lactam-synthase (EC:6.3.3.4)
Alternative name(s):
Beta-lactam synthetase
Gene namesi
Name:bls
OrganismiStreptomyces clavuligerus
Taxonomic identifieri1901 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000569391 – 513Carboxyethyl-arginine beta-lactam-synthaseAdd BLAST513

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1513
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0DJQ7
SMRiP0DJQ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the asparagine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105TCH Bacteria
COG0367 LUCA
KOiK12674

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR001962 Asn_synthase
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 2 hits
PF13537 GATase_7, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit

Sequencei

Sequence statusi: Complete.

P0DJQ7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGAPVLPAAF GFLASARTGG GRAPGPVFAT RGSHTDIDTP QGERSLAATL
60 70 80 90 100
VHAPSVAPDR AVARSLTGAP TTAVLAGEIY NRDELLSVLP AGPAPEGDAE
110 120 130 140 150
LVLRLLERYD LHAFRLVNGR FATVVRTGDR VLLATDHAGS VPLYTCVAPG
160 170 180 190 200
EVRASTEAKA LAAHRDPKGF PLADARRVAG LTGVYQVPAG AVMDIDLGSG
210 220 230 240 250
TAVTHRTWTP GLSRRILPEG EAVAAVRAAL EKAVAQRVTP GDTPLVVLSG
260 270 280 290 300
GIDSSGVAAC AHRAAGELDT VSMGTDTSNE FREARAVVDH LRTRHREITI
310 320 330 340 350
PTTELLAQLP YAVWASESVD PDIIEYLLPL TALYRALDGP ERRILTGYGA
360 370 380 390 400
DIPLGGMHRE DRLPALDTVL AHDMATFDGL NEMSPVLSTL AGHWTTHPYW
410 420 430 440 450
DREVLDLLVS LEAGLKRRHG RDKWVLRAAM ADALPAETVN RPKLGVHEGS
460 470 480 490 500
GTTSSFSRLL LDHGVAEDRV HEAKRQVVRE LFDLTVGGGR HPSEVDTDDV
510
VRSVADRTAR GAA
Length:513
Mass (Da):54,530
Last modified:September 5, 2012 - v1
Checksum:iEC2F460A77EB65CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071051 Genomic DNA Translation: AAC31901.1
X84101 Genomic DNA Translation: CAA58903.1
PIRiS57668
RefSeqiWP_003952510.1, NZ_CP032052.1

Genome annotation databases

KEGGiag:AAC31901
sclf:BB341_07810

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071051 Genomic DNA Translation: AAC31901.1
X84101 Genomic DNA Translation: CAA58903.1
PIRiS57668
RefSeqiWP_003952510.1, NZ_CP032052.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JGTX-ray1.95A/B1-513[»]
1M1ZX-ray1.95A/B1-513[»]
1MB9X-ray2.11A/B1-513[»]
1MBZX-ray2.47A/B1-513[»]
1MC1X-ray2.16A/B1-513[»]
ProteinModelPortaliP0DJQ7
SMRiP0DJQ7
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAC31901
sclf:BB341_07810

Phylogenomic databases

eggNOGiENOG4105TCH Bacteria
COG0367 LUCA
KOiK12674

Enzyme and pathway databases

UniPathwayi
UPA00112;UER00243

BioCyciMetaCyc:MONOMER-13483

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR001962 Asn_synthase
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 2 hits
PF13537 GATase_7, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBLS_STRCL
AccessioniPrimary (citable) accession number: P0DJQ7
Secondary accession number(s): Q53938, Q9R8E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: September 5, 2012
Last modified: November 7, 2018
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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