UniProtKB - P0DJJ8 (PA2B1_PROFL)
Protein
Basic phospholipase A2 BP-I
Gene
N/A
Organism
Protobothrops flavoviridis (Habu) (Trimeresurus flavoviridis)
Status
Functioni
Snake venom phospholipase A2 (PLA2) that has strong myotoxic activity with a low phospholipase A2 activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.1 Publication
Miscellaneous
Is abundantly expressed in Tokunoshima and Amami-Oshima P.flavoviridis venom, but is missing in Okinawa P.flavoviridis venom. It is thought that loss of BP-I in Okinawa P.flavoviridis is due to lack of necessity for a strong toxicity exerted by BP-I in the venom as far as they feed mostly on frogs.
Caution
Binds calcium very weakly as one of the calcium-binding ligands is lost (Asp->Lys in position 64, which corresponds to 'Lys-49' in the current nomenclature).Curated
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+PROSITE-ProRule annotationEC:3.1.1.4PROSITE-ProRule annotation
Cofactori
Ca2+1 PublicationNote: Binds 1 Ca2+ ion.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 45 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 47 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 63 | By similarity | 1 | |
Active sitei | 106 | By similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: UniProtKB-EC
- phospholipase A2 activity (consuming 1,2-dipalmitoylphosphatidylcholine) Source: UniProtKB-EC
- phospholipase A2 activity consuming 1,2-dioleoylphosphatidylethanolamine) Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- arachidonic acid secretion Source: InterPro
- lipid catabolic process Source: UniProtKB-KW
- phospholipid metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Myotoxin, Toxin |
Biological process | Lipid degradation, Lipid metabolism |
Ligand | Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Basic phospholipase A2 BP-I (EC:3.1.1.4)Short name: svPLA2 Alternative name(s): Basic protein I3 Publications Short name: BP11 Publication Short name: BPI2 Publications Phosphatidylcholine 2-acylhydrolase |
Organismi | Protobothrops flavoviridis (Habu) (Trimeresurus flavoviridis) |
Taxonomic identifieri | 88087 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Crotalinae › Protobothrops |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | 1 PublicationAdd BLAST | 16 | |
ChainiPRO_0000022953 | 17 – 138 | Basic phospholipase A2 BP-IAdd BLAST | 122 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 132 | By similarity | ||
Disulfide bondi | 44 ↔ 60 | By similarity | ||
Disulfide bondi | 59 ↔ 112 | By similarity | ||
Disulfide bondi | 65 ↔ 138 | By similarity | ||
Disulfide bondi | 66 ↔ 105 | By similarity | ||
Disulfide bondi | 73 ↔ 98 | By similarity | ||
Disulfide bondi | 91 ↔ 103 | By similarity |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0DJJ8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTLWIMAVL LLGVDGSLVQ LWKMIFQETG KEAAKNYGLY GCNCGVGRRG
60 70 80 90 100
KPKDATDSCC YVHKCCYKKV TGCDPKMDSY SYSWKNKAIV CGEKNPPCLK
110 120 130
QVCECDKAVA ICLRENLGTY NKKYTIYPKP FCKKADTC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10718 mRNA Translation: BAA01561.1 D13383 Genomic DNA Translation: BAA02651.1 |
PIRi | D48188 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10718 mRNA Translation: BAA01561.1 D13383 Genomic DNA Translation: BAA02651.1 |
PIRi | D48188 |
3D structure databases
SMRi | P0DJJ8 |
ModBasei | Search... |
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PA2B1_PROFL | |
Accessioni | P0DJJ8Primary (citable) accession number: P0DJJ8 Secondary accession number(s): B6F140, P20381 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2012 |
Last sequence update: | October 3, 2012 | |
Last modified: | February 26, 2020 | |
This is version 29 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families