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Protein

Pepsin A-5

Gene

PGA5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei94PROSITE-ProRule annotation1 Publication1
Active sitei277PROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: GO_Central
  • peptidase activity Source: Reactome

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processDigestion

Enzyme and pathway databases

ReactomeiR-HSA-5683826 Surfactant metabolism

Protein family/group databases

MEROPSiA01.071

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin A-5 (EC:3.4.23.1)
Alternative name(s):
Pepsinogen-5
Gene namesi
Name:PGA5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000256713.7
HGNCiHGNC:8887 PGA5
MIMi169700 gene
169730 gene
neXtProtiNX_P0DJD9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5222
OpenTargetsiENSG00000256713
PharmGKBiPA33224

Chemistry databases

ChEMBLiCHEMBL3295
GuidetoPHARMACOLOGYi2390

Polymorphism and mutation databases

BioMutaiPGA5
DMDMi378522017

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 152 PublicationsAdd BLAST15
PropeptideiPRO_000041575916 – 62Activation peptideAdd BLAST47
ChainiPRO_000041576063 – 388Pepsin A-5Add BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi107 ↔ 1121 Publication
Modified residuei130PhosphoserineBy similarity1
Disulfide bondi268 ↔ 2721 Publication
Disulfide bondi311 ↔ 3441 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP0DJD9
PeptideAtlasiP0DJD9
PRIDEiP0DJD9
ProteomicsDBi52546

PTM databases

iPTMnetiP0DJD9
PhosphoSitePlusiP0DJD9

Expressioni

Gene expression databases

BgeeiENSG00000256713 Expressed in 82 organ(s), highest expression level in fundus of stomach
CleanExiHS_PGA5
ExpressionAtlasiP0DJD9 baseline and differential
GenevisibleiP0DJD9 HS

Organism-specific databases

HPAiHPA046875

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000309542

Chemistry databases

BindingDBiP0DJD9

Structurei

3D structure databases

ProteinModelPortaliP0DJD9
SMRiP0DJD9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 385Peptidase A1PROSITE-ProRule annotationAdd BLAST310

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
InParanoidiP0DJD9
KOiK06002
OMAiIRQYFAV
OrthoDBiEOG091G0JP7
PhylomeDBiP0DJD9
TreeFamiTF314990

Family and domain databases

CDDicd05478 pepsin_A, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR034162 Pepsin_A
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P0DJD9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKWLLLLGLV ALSECIMYKV PLIRKKSLRR TLSERGLLKD FLKKHNLNPA
60 70 80 90 100
RKYFPQWEAP TLVDEQPLEN YLDMEYFGTI GIGTPAQDFT VVFDTGSSNL
110 120 130 140 150
WVPSVYCSSL ACTNHNRFNP EDSSTYQSTS ETVSITYGTG SMTGILGYDT
160 170 180 190 200
VQVGGISDTN QIFGLSETEP GSFLYYAPFD GILGLAYPSI SSSGATPVFD
210 220 230 240 250
NIWNQGLVSQ DLFSVYLSAD DKSGSVVIFG GIDSSYYTGS LNWVPVTVEG
260 270 280 290 300
YWQITVDSIT MNGETIACAE GCQAIVDTGT SLLTGPTSPI ANIQSDIGAS
310 320 330 340 350
ENSDGDMVVS CSAISSLPDI VFTINGVQYP VPPSAYILQS EGSCISGFQG
360 370 380
MNVPTESGEL WILGDVFIRQ YFTVFDRANN QVGLAPVA
Length:388
Mass (Da):41,993
Last modified:February 22, 2012 - v1
Checksum:i2C456A75DA96CDDA
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JM59C9JM59_HUMAN
Pepsin A-5
PGA5
234Annotation score:
F5GWT0F5GWT0_HUMAN
Pepsin A-5
PGA5
128Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28L → F in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti58E → K in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti92V → L in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti222K → Q in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti265T → A in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti353V → L in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti371 – 372YF → FY AA sequence (PubMed:4909888).Curated2
Sequence conflicti376D → E in AAA60061 (PubMed:2714789).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26032
, M26025, M26026, M26027, M26028, M26029, M26030, M26031 Genomic DNA Translation: AAA60061.1
AP003037 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW73928.1
BC029055 mRNA Translation: AAH29055.1
BC146999 mRNA Translation: AAI47000.1
BC171889 mRNA Translation: AAI71889.1
CCDSiCCDS8001.1
PIRiA00980 PEHU
A30142
A92058
B30142
RefSeqiNP_055039.1, NM_014224.4
UniGeneiHs.432854

Genome annotation databases

EnsembliENST00000312403; ENSP00000309542; ENSG00000256713
GeneIDi5222
KEGGihsa:5222
UCSCiuc001nqz.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26032
, M26025, M26026, M26027, M26028, M26029, M26030, M26031 Genomic DNA Translation: AAA60061.1
AP003037 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW73928.1
BC029055 mRNA Translation: AAH29055.1
BC146999 mRNA Translation: AAI47000.1
BC171889 mRNA Translation: AAI71889.1
CCDSiCCDS8001.1
PIRiA00980 PEHU
A30142
A92058
B30142
RefSeqiNP_055039.1, NM_014224.4
UniGeneiHs.432854

3D structure databases

ProteinModelPortaliP0DJD9
SMRiP0DJD9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000309542

Chemistry databases

BindingDBiP0DJD9
ChEMBLiCHEMBL3295
GuidetoPHARMACOLOGYi2390

Protein family/group databases

MEROPSiA01.071

PTM databases

iPTMnetiP0DJD9
PhosphoSitePlusiP0DJD9

Polymorphism and mutation databases

BioMutaiPGA5
DMDMi378522017

Proteomic databases

PaxDbiP0DJD9
PeptideAtlasiP0DJD9
PRIDEiP0DJD9
ProteomicsDBi52546

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312403; ENSP00000309542; ENSG00000256713
GeneIDi5222
KEGGihsa:5222
UCSCiuc001nqz.4 human

Organism-specific databases

CTDi5222
DisGeNETi5222
EuPathDBiHostDB:ENSG00000256713.7
GeneCardsiPGA5
HGNCiHGNC:8887 PGA5
HPAiHPA046875
MIMi169700 gene
169730 gene
neXtProtiNX_P0DJD9
OpenTargetsiENSG00000256713
PharmGKBiPA33224
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
InParanoidiP0DJD9
KOiK06002
OMAiIRQYFAV
OrthoDBiEOG091G0JP7
PhylomeDBiP0DJD9
TreeFamiTF314990

Enzyme and pathway databases

ReactomeiR-HSA-5683826 Surfactant metabolism

Miscellaneous databases

ChiTaRSiPGA5 human
GeneWikiiPGA5
GenomeRNAii5222
PROiPR:P0DJD9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000256713 Expressed in 82 organ(s), highest expression level in fundus of stomach
CleanExiHS_PGA5
ExpressionAtlasiP0DJD9 baseline and differential
GenevisibleiP0DJD9 HS

Family and domain databases

CDDicd05478 pepsin_A, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR034162 Pepsin_A
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPEPA5_HUMAN
AccessioniPrimary (citable) accession number: P0DJD9
Secondary accession number(s): A8K749
, B7ZW62, B7ZW75, P00790, Q7M4R0, Q8N1E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: February 22, 2012
Last modified: November 7, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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