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Protein

Pepsin A-3

Gene

PGA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei94PROSITE-ProRule annotation1 Publication1
Active sitei277PROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: GO_Central
  • peptidase activity Source: Reactome

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processDigestion

Enzyme and pathway databases

ReactomeiR-HSA-5683826 Surfactant metabolism

Protein family/group databases

MEROPSiA01.001

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin A-3 (EC:3.4.23.1)
Alternative name(s):
Pepsinogen-3
Gene namesi
Name:PGA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000229859.8
HGNCiHGNC:8885 PGA3
MIMi169700 gene
169710 gene
neXtProtiNX_P0DJD8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi643834
OpenTargetsiENSG00000229859

Polymorphism and mutation databases

BioMutaiPGA3
DMDMi378521956

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 152 PublicationsAdd BLAST15
PropeptideiPRO_000002601316 – 62Activation peptideAdd BLAST47
ChainiPRO_000002601463 – 388Pepsin A-3Add BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi107 ↔ 1121 Publication
Disulfide bondi268 ↔ 2721 Publication
Disulfide bondi311 ↔ 3441 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP0DJD8
PeptideAtlasiP0DJD8
PRIDEiP0DJD8
ProteomicsDBi52545

PTM databases

iPTMnetiP0DJD8
PhosphoSitePlusiP0DJD8

Expressioni

Gene expression databases

BgeeiENSG00000229859 Expressed in 83 organ(s), highest expression level in fundus of stomach
CleanExiHS_PGA3
ExpressionAtlasiP0DJD8 baseline and differential
GenevisibleiP0DJD8 HS

Organism-specific databases

HPAiCAB026358
HPA046875

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000322192

Structurei

3D structure databases

ProteinModelPortaliP0DJD8
SMRiP0DJD8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 385Peptidase A1PROSITE-ProRule annotationAdd BLAST310

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
InParanoidiP0DJD8
KOiK06002
OMAiHHHYDIG
OrthoDBiEOG091G0JP7
PhylomeDBiP0DJD8
TreeFamiTF314990

Family and domain databases

CDDicd05478 pepsin_A, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR034162 Pepsin_A
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DJD8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKWLLLLGLV ALSECIMYKV PLIRKKSLRR TLSERGLLKD FLKKHNLNPA
60 70 80 90 100
RKYFPQWKAP TLVDEQPLEN YLDMEYFGTI GIGTPAQDFT VVFDTGSSNL
110 120 130 140 150
WVPSVYCSSL ACTNHNRFNP EDSSTYQSTS ETVSITYGTG SMTGILGYDT
160 170 180 190 200
VQVGGISDTN QIFGLSETEP GSFLYYAPFD GILGLAYPSI SSSGATPVFD
210 220 230 240 250
NIWNQGLVSQ DLFSVYLSAD DQSGSVVIFG GIDSSYYTGS LNWVPVTVEG
260 270 280 290 300
YWQITVDSIT MNGEAIACAE GCQAIVDTGT SLLTGPTSPI ANIQSDIGAS
310 320 330 340 350
ENSDGDMVVS CSAISSLPDI VFTINGVQYP VPPSAYILQS EGSCISGFQG
360 370 380
MNLPTESGEL WILGDVFIRQ YFTVFDRANN QVGLAPVA
Length:388
Mass (Da):41,976
Last modified:February 22, 2012 - v1
Checksum:i5DA16E41FE198582
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92V → L in BAG35783 (PubMed:14702039).Curated1
Sequence conflicti92V → L in BAG54225 (PubMed:14702039).Curated1
Sequence conflicti92V → L in AAI71815 (PubMed:15489334).Curated1
Sequence conflicti371 – 372YF → FY AA sequence (PubMed:4909888).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK312941 mRNA Translation: BAG35783.1
AK125628 mRNA Translation: BAG54225.1
AP000437 Genomic DNA No translation available.
BC171815 mRNA Translation: AAI71815.1
CCDSiCCDS31574.1
PIRiA00980 PEHU
A30142
A92058
B30142
RefSeqiNP_001073275.1, NM_001079807.3
UniGeneiHs.601055
Hs.661883

Genome annotation databases

EnsembliENST00000325558; ENSP00000322192; ENSG00000229859
GeneIDi643834
KEGGihsa:643834
UCSCiuc001nqx.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK312941 mRNA Translation: BAG35783.1
AK125628 mRNA Translation: BAG54225.1
AP000437 Genomic DNA No translation available.
BC171815 mRNA Translation: AAI71815.1
CCDSiCCDS31574.1
PIRiA00980 PEHU
A30142
A92058
B30142
RefSeqiNP_001073275.1, NM_001079807.3
UniGeneiHs.601055
Hs.661883

3D structure databases

ProteinModelPortaliP0DJD8
SMRiP0DJD8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000322192

Protein family/group databases

MEROPSiA01.001

PTM databases

iPTMnetiP0DJD8
PhosphoSitePlusiP0DJD8

Polymorphism and mutation databases

BioMutaiPGA3
DMDMi378521956

Proteomic databases

PaxDbiP0DJD8
PeptideAtlasiP0DJD8
PRIDEiP0DJD8
ProteomicsDBi52545

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325558; ENSP00000322192; ENSG00000229859
GeneIDi643834
KEGGihsa:643834
UCSCiuc001nqx.4 human

Organism-specific databases

CTDi643834
DisGeNETi643834
EuPathDBiHostDB:ENSG00000229859.8
GeneCardsiPGA3
HGNCiHGNC:8885 PGA3
HPAiCAB026358
HPA046875
MIMi169700 gene
169710 gene
neXtProtiNX_P0DJD8
OpenTargetsiENSG00000229859
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
InParanoidiP0DJD8
KOiK06002
OMAiHHHYDIG
OrthoDBiEOG091G0JP7
PhylomeDBiP0DJD8
TreeFamiTF314990

Enzyme and pathway databases

ReactomeiR-HSA-5683826 Surfactant metabolism

Miscellaneous databases

ChiTaRSiPGA3 human
GenomeRNAii643834
PROiPR:P0DJD8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000229859 Expressed in 83 organ(s), highest expression level in fundus of stomach
CleanExiHS_PGA3
ExpressionAtlasiP0DJD8 baseline and differential
GenevisibleiP0DJD8 HS

Family and domain databases

CDDicd05478 pepsin_A, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR034162 Pepsin_A
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPEPA3_HUMAN
AccessioniPrimary (citable) accession number: P0DJD8
Secondary accession number(s): A8K749
, B2R7D6, B7ZW75, P00790, Q7M4R0, Q8N1E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: February 22, 2012
Last modified: November 7, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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