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UniProtKB - P0DJD7 (PEPA4_HUMAN)
Protein
Pepsin A-4
Gene
PGA4
Organism
Homo sapiens (Human)
Status
Functioni
Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Catalytic activityi
- Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.PROSITE-ProRule annotation EC:3.4.23.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 94 | PROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 277 | PROSITE-ProRule annotation1 Publication | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-KW
GO - Biological processi
- digestion Source: UniProtKB-KW
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Biological process | Digestion |
Enzyme and pathway databases
PathwayCommonsi | P0DJD7 |
Reactomei | R-HSA-5683826, Surfactant metabolism |
SignaLinki | P0DJD7 |
Protein family/group databases
MEROPSi | A01.070 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PGA4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8886, PGA4 |
MIMi | 169700, gene 169720, gene |
neXtProti | NX_P0DJD7 |
VEuPathDBi | HostDB:ENSG00000229183 |
Subcellular locationi
Extracellular region or secreted
Endosome
- multivesicular body lumen Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 643847 |
OpenTargetsi | ENSG00000229183 |
Miscellaneous databases
Pharosi | P0DJD7, Tbio |
Genetic variation databases
BioMutai | PGA4 |
DMDMi | 378521995 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | 2 PublicationsAdd BLAST | 15 | |
PropeptideiPRO_0000415757 | 16 – 62 | Activation peptideAdd BLAST | 47 | |
ChainiPRO_0000415758 | 63 – 388 | Pepsin A-4Add BLAST | 326 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 107 ↔ 112 | 1 Publication | ||
Modified residuei | 130 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 268 ↔ 272 | 1 Publication | ||
Disulfide bondi | 311 ↔ 344 | 1 Publication |
Keywords - PTMi
Disulfide bond, Phosphoprotein, ZymogenProteomic databases
jPOSTi | P0DJD7 |
MassIVEi | P0DJD7 |
PaxDbi | P0DJD7 |
PeptideAtlasi | P0DJD7 |
PRIDEi | P0DJD7 |
PTM databases
iPTMneti | P0DJD7 |
PhosphoSitePlusi | P0DJD7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000229183, Expressed in fundus of stomach and 101 other tissues |
ExpressionAtlasi | P0DJD7, baseline and differential |
Genevisiblei | P0DJD7, HS |
Organism-specific databases
HPAi | ENSG00000229183, Tissue enriched (stomach) |
Interactioni
Binary interactionsi
P0DJD7
Protein-protein interaction databases
BioGRIDi | 569099, 10 interactors |
IntActi | P0DJD7, 9 interactors |
STRINGi | 9606.ENSP00000367391 |
Chemistry databases
BindingDBi | P0DJD7 |
Miscellaneous databases
RNActi | P0DJD7, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0DJD7 |
SMRi | P0DJD7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 76 – 385 | Peptidase A1PROSITE-ProRule annotationAdd BLAST | 310 |
Sequence similaritiesi
Belongs to the peptidase A1 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1339, Eukaryota |
GeneTreei | ENSGT00940000155036 |
HOGENOMi | CLU_013253_3_0_1 |
InParanoidi | P0DJD7 |
OMAi | ENYMDME |
OrthoDBi | 1619495at2759 |
PhylomeDBi | P0DJD7 |
TreeFami | TF314990 |
Family and domain databases
CDDi | cd05478, pepsin_A, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR012848, Aspartic_peptidase_N IPR034162, Pepsin_A IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF07966, A1_Propeptide, 1 hit PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P0DJD7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKWLLLLGLV ALSECIMYKV PLIRKKSLRR TLSERGLLKD FLKKHNLNPA
60 70 80 90 100
RKYFPQWEAP TLVDEQPLEN YLDMEYFGTI GIGTPAQDFT VVFDTGSSNL
110 120 130 140 150
WVPSVYCSSL ACTNHNRFNP EDSSTYQSTS ETVSITYGTG SMTGILGYDT
160 170 180 190 200
VQVGGISDTN QIFGLSETEP GSFLYYAPFD GILGLAYPSI SSSGATPVFD
210 220 230 240 250
NIWNQGLVSQ DLFSVYLSAD DQSGSVVIFG GIDSSYYTGS LNWVPVTVEG
260 270 280 290 300
YWQITVDSIT MNGEAIACAE GCQAIVDTGT SLLTGPTSPI ANIQSDIGAS
310 320 330 340 350
ENSDGDMVVS CSAISSLPDI VFTINGVQYP VPPSAYILQS EGSCISGFQG
360 370 380
MNLPTESGEL WILGDVFIRQ YFTVFDRANN QVGLAPVA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5GXL4 | F5GXL4_HUMAN | Pepsin A-3 | PGA3 PGA4 | 234 | Annotation score: | ||
F5H0H6 | F5H0H6_HUMAN | Pepsin A-3 | PGA4 PGA3 | 128 | Annotation score: | ||
F5H842 | F5H842_HUMAN | Pepsin A-3 | PGA3 | 265 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 291 | A → T in BAF84553 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 371 – 372 | YF → FY AA sequence (PubMed:4909888).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J00287 , J00279, J00280, J00281, J00282, J00283, J00284, J00285, J00286 Genomic DNA Translation: AAA98529.1 AK291864 mRNA Translation: BAF84553.1 AP000437 Genomic DNA No translation available. AP003037 Genomic DNA No translation available. BC150659 mRNA Translation: AAI50660.1 BC171910 mRNA Translation: AAI71910.1 BC171920 mRNA Translation: AAI71920.1 |
CCDSi | CCDS31575.1 |
PIRi | A00980, PEHU A30142 A92058 B30142 |
RefSeqi | NP_001073276.1, NM_001079808.3 XP_016854970.1, XM_016999481.1 |
Genome annotation databases
Ensembli | ENST00000378149.9; ENSP00000367391.3; ENSG00000229183.10 |
GeneIDi | 643847 |
KEGGi | hsa:643847 |
MANE-Selecti | ENST00000378149.9; ENSP00000367391.3; NM_001079808.6; NP_001073276.1 |
UCSCi | uc001nqy.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J00287 , J00279, J00280, J00281, J00282, J00283, J00284, J00285, J00286 Genomic DNA Translation: AAA98529.1 AK291864 mRNA Translation: BAF84553.1 AP000437 Genomic DNA No translation available. AP003037 Genomic DNA No translation available. BC150659 mRNA Translation: AAI50660.1 BC171910 mRNA Translation: AAI71910.1 BC171920 mRNA Translation: AAI71920.1 |
CCDSi | CCDS31575.1 |
PIRi | A00980, PEHU A30142 A92058 B30142 |
RefSeqi | NP_001073276.1, NM_001079808.3 XP_016854970.1, XM_016999481.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FLH | X-ray | 2.45 | A | 63-388 | [»] | |
1PSN | X-ray | 2.20 | A | 63-388 | [»] | |
1PSO | X-ray | 2.00 | E | 63-388 | [»] | |
1QRP | X-ray | 1.96 | E | 63-388 | [»] | |
3UTL | X-ray | 2.61 | A | 63-388 | [»] | |
AlphaFoldDBi | P0DJD7 | |||||
SMRi | P0DJD7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 569099, 10 interactors |
IntActi | P0DJD7, 9 interactors |
STRINGi | 9606.ENSP00000367391 |
Chemistry databases
BindingDBi | P0DJD7 |
Protein family/group databases
MEROPSi | A01.070 |
PTM databases
iPTMneti | P0DJD7 |
PhosphoSitePlusi | P0DJD7 |
Genetic variation databases
BioMutai | PGA4 |
DMDMi | 378521995 |
Proteomic databases
jPOSTi | P0DJD7 |
MassIVEi | P0DJD7 |
PaxDbi | P0DJD7 |
PeptideAtlasi | P0DJD7 |
PRIDEi | P0DJD7 |
Protocols and materials databases
Antibodypediai | 28082, 138 antibodies from 21 providers |
DNASUi | 643847 |
Genome annotation databases
Ensembli | ENST00000378149.9; ENSP00000367391.3; ENSG00000229183.10 |
GeneIDi | 643847 |
KEGGi | hsa:643847 |
MANE-Selecti | ENST00000378149.9; ENSP00000367391.3; NM_001079808.6; NP_001073276.1 |
UCSCi | uc001nqy.4, human |
Organism-specific databases
CTDi | 643847 |
DisGeNETi | 643847 |
GeneCardsi | PGA4 |
HGNCi | HGNC:8886, PGA4 |
HPAi | ENSG00000229183, Tissue enriched (stomach) |
MIMi | 169700, gene 169720, gene |
neXtProti | NX_P0DJD7 |
OpenTargetsi | ENSG00000229183 |
VEuPathDBi | HostDB:ENSG00000229183 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1339, Eukaryota |
GeneTreei | ENSGT00940000155036 |
HOGENOMi | CLU_013253_3_0_1 |
InParanoidi | P0DJD7 |
OMAi | ENYMDME |
OrthoDBi | 1619495at2759 |
PhylomeDBi | P0DJD7 |
TreeFami | TF314990 |
Enzyme and pathway databases
PathwayCommonsi | P0DJD7 |
Reactomei | R-HSA-5683826, Surfactant metabolism |
SignaLinki | P0DJD7 |
Miscellaneous databases
BioGRID-ORCSi | 643847, 4 hits in 206 CRISPR screens |
ChiTaRSi | PGA4, human |
GenomeRNAii | 643847 |
Pharosi | P0DJD7, Tbio |
PROi | PR:P0DJD7 |
RNActi | P0DJD7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000229183, Expressed in fundus of stomach and 101 other tissues |
ExpressionAtlasi | P0DJD7, baseline and differential |
Genevisiblei | P0DJD7, HS |
Family and domain databases
CDDi | cd05478, pepsin_A, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR012848, Aspartic_peptidase_N IPR034162, Pepsin_A IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF07966, A1_Propeptide, 1 hit PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PEPA4_HUMAN | |
Accessioni | P0DJD7Primary (citable) accession number: P0DJD7 Secondary accession number(s): A8K749 Q8N1E3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 22, 2012 |
Last sequence update: | February 22, 2012 | |
Last modified: | May 25, 2022 | |
This is version 77 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families