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Protein

Oligo-1,6-glucosidase IMA3

Gene

IMA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha-glucosidase with broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides. Not required for isomaltose utilization, but overexpression allows the IMA1 null mutant to grow on isomaltose.1 Publication

Miscellaneous

Present with 166 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei215NucleophileBy similarity1
Active sitei277Proton donorBy similarity1
Sitei352Transition state stabilizerBy similarity1

GO - Molecular functioni

  • oligo-1,6-glucosidase activity Source: SGD
  • sucrose alpha-glucosidase activity Source: SGD

GO - Biological processi

  • disaccharide catabolic process Source: SGD
  • maltose metabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processMaltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YIL172C-MONOMER

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA3 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Isomaltase 3
Gene namesi
Name:IMA3
Ordered Locus Names:YIL172C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

SGDiS000001434 IMA3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543321 – 589Oligo-1,6-glucosidase IMA3Add BLAST589

Proteomic databases

PaxDbiP0CW40
PRIDEiP0CW40

Interactioni

Protein-protein interaction databases

BioGridi33561, 14 interactors
34824, 5 interactors
STRINGi4932.YJL221C

Structurei

3D structure databases

ProteinModelPortaliP0CW40
SMRiP0CW40
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

HOGENOMiHOG000220641
InParanoidiP0CW40
KOiK01182
OMAiSSAHRWF
OrthoDBiEOG092C12JX

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

P0CW40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISSAHPET EPKWWKEATI YQIYPASFKD SNNDGWGDMK GIASKLEYIK
60 70 80 90 100
ELGTDAIWIS PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL
110 120 130 140 150
GMKFITDLVI NHCSSEHEWF KESRSSKTNP KRDWFFWRPP KGYDAEGKPI
160 170 180 190 200
PPNNWRSYFG GSAWTFDEKT QEFYLRLFCS TQPDLNWENE DCRKAIYESA
210 220 230 240 250
VGYWLDHGVD GFRIDVGSLY SKVAGLPDAP VIDENSKWQL SDPFTMNGPR
260 270 280 290 300
IHEFHQEMNK FIRNRVKDGR EIMTVGEMRH ATDETKRLYT SASRHELSEL
310 320 330 340 350
FNFSHTDVGT SPKFRQNLIP YELKDWKVAL AELFRYVNGT DCWSTIYLEN
360 370 380 390 400
HDQPRSITRF GDDSPKNRVI SGKLLSVLLV SLSGTLYVYQ GQELGEINFK
410 420 430 440 450
NWPIEKYEDV EVRNNYDAIK EEHGENSKEM KRFLEAIALI SRDHARTPMQ
460 470 480 490 500
WSREEPNAGF SGPNAKPWFY LNESFREGIN AEDESKDPNS VLNFWKEALR
510 520 530 540 550
FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYDNKTLFAA LNFSSDSIDF
560 570 580
TIPNNSSSFK LEFGNYPRSE VDASSRTLKP WEGRIYISE
Length:589
Mass (Da):68,699
Last modified:May 3, 2011 - v1
Checksum:i0323A2677148EC29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46921 Genomic DNA Translation: CAA87020.1
BK006942 Genomic DNA Translation: DAA08385.1
PIRiS50355
RefSeqiNP_012096.1, NM_001179518.1
NP_012314.1, NM_001181654.1

Genome annotation databases

EnsemblFungiiYIL172C; YIL172C; YIL172C
YJL221C; YJL221C; YJL221C
GeneIDi853235
854635
KEGGisce:YIL172C
sce:YJL221C

Similar proteinsi

Entry informationi

Entry nameiMALX2_YEAST
AccessioniPrimary (citable) accession number: P0CW40
Secondary accession number(s): D6VVX6, P40439
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: March 28, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

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