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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144Zinc 1UniRule annotation1
Metal bindingi147Zinc 1UniRule annotation1
Metal bindingi161Zinc 2UniRule annotation1
Metal bindingi164Zinc 2UniRule annotation1
Metal bindingi187Zinc 2UniRule annotation1
Metal bindingi190Zinc 2UniRule annotation1
Metal bindingi201Zinc 1UniRule annotation1
Metal bindingi204Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri131 – 213CR-typeUniRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:MM_2504
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004080361 – 389Chaperone protein DnaJAdd BLAST389

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi192952.MM_2504

Structurei

3D structure databases

ProteinModelPortaliP0CW07
SMRiP0CW07
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 70JUniRule annotationAdd BLAST65
Repeati144 – 151CXXCXGXG motif8
Repeati161 – 168CXXCXGXG motif8
Repeati187 – 194CXXCXGXG motif8
Repeati201 – 208CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 114Gly-richAdd BLAST40

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri131 – 213CR-typeUniRule annotationAdd BLAST83

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiarCOG02846 Archaea
COG0484 LUCA
HOGENOMiHOG000226717
KOiK03686
OMAiDMGGFAD
OrthoDBiPOG093Z045I

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd10719 DnaJ_zf, 1 hit
Gene3Di1.10.287.110, 1 hit
HAMAPiMF_01152 DnaJ, 1 hit
InterProiView protein in InterPro
IPR012724 DnaJ
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 3 hits
SSF57938 SSF57938, 1 hit
TIGRFAMsiTIGR02349 DnaJ_bact, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS51188 ZF_CR, 1 hit

Sequencei

Sequence statusi: Complete.

P0CW07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKRDYYEI LGLSKDSSVE DIKKTYRKLA LQYHPDRNKE PGAEEKFKEI
60 70 80 90 100
SEAYAVLSDA EKRAQYDRFG HAGIDNQYSA EDIFRGADFG GFGDIFEMFF
110 120 130 140 150
GGGRRGGPMG PRRGSDLQYD LYVTFEEAAF GVRKDIDIPR TERCSTCSGT
160 170 180 190 200
GAKPGTSPKR CPNCGGTGQV RTTRSTLGMQ FVSTTTCSAC HGRGQVVESP
210 220 230 240 250
CPTCSGAGRV RSRRKMSVNV PAGADSGMTL RLSGEGDAGE PGAPSGDLYI
260 270 280 290 300
IVHVMEHKYF KRVDYDVISE LPISFTQAAL GADIMVDTLY GKVKMNIPSG
310 320 330 340 350
TQTHSVFRLK DKGIQRLQGH GKGDQLVRVI IRTPTKLTQE QKDLLHQFEY
360 370 380
LSNGKKPEAE ERSRSDKQKS EKPRKSKGLF EKVKDAFES
Length:389
Mass (Da):42,990
Last modified:May 3, 2011 - v1
Checksum:i5BE40E379D8C8139
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA Translation: AAM32200.1
RefSeqiWP_011034422.1, NC_003901.1

Genome annotation databases

EnsemblBacteriaiAAM32200; AAM32200; MM_2504
GeneIDi24882229
KEGGimma:MM_2504
PATRICifig|192952.21.peg.2867

Similar proteinsi

Entry informationi

Entry nameiDNAJ_METMA
AccessioniPrimary (citable) accession number: P0CW07
Secondary accession number(s): P35515
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: July 18, 2018
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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