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Protein

Isocitrate lyase

Gene

ICL1

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate (By similarity). plays an important role in plant pathogenicity.By similarity1 Publication

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (ICL1)
  2. Malate synthase (MGG_02813)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi177MagnesiumBy similarity1
Active sitei215Proton acceptorBy similarity1
Binding sitei252SubstrateBy similarity1
Binding sitei467SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayi
UPA00703;UER00719

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyase1 Publication (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:ICL11 Publication
ORF Names:MGG_04895
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
Proteomesi
  • UP000009058 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiFungiDB:MGG_04895

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Glyoxysome, Peroxisome

Pathology & Biotechi

Disruption phenotypei

Leads to reduced virulence and a delay in the expression of rice blast symptoms.1 Publication

Chemistry databases

ChEMBLiCHEMBL1075264

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000687931 – 547Isocitrate lyaseAdd BLAST547

Proteomic databases

PRIDEiP0CT06

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi318829.MGG_04895T0

Chemistry databases

BindingDBiP0CT06

Structurei

Secondary structure

1547
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0CT06
SMRiP0CT06
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 108Substrate bindingBy similarity3
Regioni216 – 217Substrate bindingBy similarity2
Regioni433 – 437Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

KOiK01637
OMAiLEKDWAE
OrthoDBiEOG092C19UX

Family and domain databases

InterProiView protein in InterPro
IPR006254 Isocitrate_lyase
IPR018523 Isocitrate_lyase_ph_CS
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR21631 PTHR21631, 1 hit
PfamiView protein in Pfam
PF00463 ICL, 1 hit
PIRSFiPIRSF001362 Isocit_lyase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR01346 isocit_lyase, 1 hit
PROSITEiView protein in PROSITE
PS00161 ISOCITRATE_LYASE, 1 hit

Sequencei

Sequence statusi: Complete.

P0CT06-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASKNMVNPA VEPSMEDDLF AREVAEVKQW WSDPRWRYTK RPFTAEQIVS
60 70 80 90 100
KRGNLKIEYP SNAQSKKLWK ILEGRFQKRD ASYTYGCLEP TMVTQMAKYL
110 120 130 140 150
DTVYVSGWQS SSTASSSDEP GPDLADYPYT TVPNKVSHLF MAQLFHDRKQ
160 170 180 190 200
RHERLSAPKS ERSKLQNIDY LRPIIADADT GHGGLTAVMK LTKLFIEKGA
210 220 230 240 250
AGIHIEDQAP GTKKCGHMAG KVLVPISEHI NRLVAIRAQA DIMGVDLLAI
260 270 280 290 300
ARTDAEAATL ITTSIDPRDH AFILGCTNPS LQPLADLMNT AEQSGKTGDQ
310 320 330 340 350
LQAIEDEWMA KANLKRFDDA VVDVINSSSS IRNPKDVAAK YLQAAKGKSN
360 370 380 390 400
REARAIASSL GVPEIFFDWD SPRTREGYFR IKGGCDCAIN RAIAYAPYAD
410 420 430 440 450
AIWMESKLPD YEQAKEFAEG VHAVYPEQKL AYNLSPSFNW KTAMPRDEQE
460 470 480 490 500
TYIRRLAGLG YCWQFITLAG LHTTALISDR FARAYSEVGM RAYGELVQEP
510 520 530 540
EMELGVDVVK HQKWSGATYV DELQKMVTGG VSSTAAMGKG VTEDQFH
Length:547
Mass (Da):61,001
Last modified:September 18, 2013 - v1
Checksum:i0BF3F9921E04D1D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF540383 Genomic DNA Translation: AAN28719.1
CM001233 Genomic DNA Translation: EHA52574.1
RefSeqiXP_003712381.1, XM_003712333.1

Genome annotation databases

EnsemblFungiiMGG_04895T0; MGG_04895T0; MGG_04895
GeneIDi2675603
KEGGimgr:MGG_04895

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF540383 Genomic DNA Translation: AAN28719.1
CM001233 Genomic DNA Translation: EHA52574.1
RefSeqiXP_003712381.1, XM_003712333.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5E9FX-ray2.80A/B/C/D1-547[»]
5E9GX-ray2.10A/B/C/D1-547[»]
ProteinModelPortaliP0CT06
SMRiP0CT06
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi318829.MGG_04895T0

Chemistry databases

BindingDBiP0CT06
ChEMBLiCHEMBL1075264

Proteomic databases

PRIDEiP0CT06

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiMGG_04895T0; MGG_04895T0; MGG_04895
GeneIDi2675603
KEGGimgr:MGG_04895

Organism-specific databases

EuPathDBiFungiDB:MGG_04895

Phylogenomic databases

KOiK01637
OMAiLEKDWAE
OrthoDBiEOG092C19UX

Enzyme and pathway databases

UniPathwayi
UPA00703;UER00719

Miscellaneous databases

PROiPR:P0CT06

Family and domain databases

InterProiView protein in InterPro
IPR006254 Isocitrate_lyase
IPR018523 Isocitrate_lyase_ph_CS
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR21631 PTHR21631, 1 hit
PfamiView protein in Pfam
PF00463 ICL, 1 hit
PIRSFiPIRSF001362 Isocit_lyase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR01346 isocit_lyase, 1 hit
PROSITEiView protein in PROSITE
PS00161 ISOCITRATE_LYASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACEA_MAGO7
AccessioniPrimary (citable) accession number: P0CT06
Secondary accession number(s): A4QU17
, G4N2R9, Q5EN15, Q8J232
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: September 18, 2013
Last modified: November 7, 2018
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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