UniProtKB - P0CT06 (ACEA_MAGO7)
Protein
Isocitrate lyase
Gene
ICL1
Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Functioni
Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate (By similarity). plays an important role in plant pathogenicity.By similarity1 Publication
Catalytic activityi
Cofactori
Mg2+By similarity
: glyoxylate cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Isocitrate lyase (ICL1)
- Malate synthase (MGG_02813)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 177 | MagnesiumBy similarity | 1 | |
Active sitei | 215 | Proton acceptorBy similarity | 1 | |
Binding sitei | 252 | SubstrateBy similarity | 1 | |
Binding sitei | 467 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- isocitrate lyase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- methylisocitrate lyase activity Source: UniProtKB-EC
GO - Biological processi
- glyoxylate cycle Source: UniProtKB-UniPathway
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Lyase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00719 |
Names & Taxonomyi
Protein namesi | Recommended name: Isocitrate lyase1 Publication (EC:4.1.3.1By similarity)Short name: ICLCurated Short name: IsocitraseCurated Short name: IsocitrataseCurated Alternative name(s): Threo-D(S)-isocitrate glyoxylate-lyaseCurated |
Gene namesi | Name:ICL11 Publication ORF Names:MGG_04895 |
Organismi | Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
Taxonomic identifieri | 242507 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Pyriculariaceae › Pyricularia › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:MGG_04895 |
Subcellular locationi
Peroxisome
- Glyoxysome By similarity
Peroxisome
- glyoxysome Source: UniProtKB-SubCell
Keywords - Cellular componenti
Glyoxysome, PeroxisomePathology & Biotechi
Disruption phenotypei
Leads to reduced virulence and a delay in the expression of rice blast symptoms.1 Publication
Miscellaneous databases
PHI-basei | PHI:305 |
Chemistry databases
ChEMBLi | CHEMBL1075264 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068793 | 1 – 547 | Isocitrate lyaseAdd BLAST | 547 |
Proteomic databases
PRIDEi | P0CT06 |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 318829.MGG_04895T0 |
Chemistry databases
BindingDBi | P0CT06 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0CT06 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 106 – 108 | Substrate bindingBy similarity | 3 | |
Regioni | 216 – 217 | Substrate bindingBy similarity | 2 | |
Regioni | 433 – 437 | Substrate bindingBy similarity | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1260, Eukaryota |
HOGENOMi | CLU_019214_2_2_1 |
OMAi | LEKDWAE |
OrthoDBi | 905115at2759 |
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 1 hit |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P0CT06-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASKNMVNPA VEPSMEDDLF AREVAEVKQW WSDPRWRYTK RPFTAEQIVS
60 70 80 90 100
KRGNLKIEYP SNAQSKKLWK ILEGRFQKRD ASYTYGCLEP TMVTQMAKYL
110 120 130 140 150
DTVYVSGWQS SSTASSSDEP GPDLADYPYT TVPNKVSHLF MAQLFHDRKQ
160 170 180 190 200
RHERLSAPKS ERSKLQNIDY LRPIIADADT GHGGLTAVMK LTKLFIEKGA
210 220 230 240 250
AGIHIEDQAP GTKKCGHMAG KVLVPISEHI NRLVAIRAQA DIMGVDLLAI
260 270 280 290 300
ARTDAEAATL ITTSIDPRDH AFILGCTNPS LQPLADLMNT AEQSGKTGDQ
310 320 330 340 350
LQAIEDEWMA KANLKRFDDA VVDVINSSSS IRNPKDVAAK YLQAAKGKSN
360 370 380 390 400
REARAIASSL GVPEIFFDWD SPRTREGYFR IKGGCDCAIN RAIAYAPYAD
410 420 430 440 450
AIWMESKLPD YEQAKEFAEG VHAVYPEQKL AYNLSPSFNW KTAMPRDEQE
460 470 480 490 500
TYIRRLAGLG YCWQFITLAG LHTTALISDR FARAYSEVGM RAYGELVQEP
510 520 530 540
EMELGVDVVK HQKWSGATYV DELQKMVTGG VSSTAAMGKG VTEDQFH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF540383 Genomic DNA Translation: AAN28719.1 CM001233 Genomic DNA Translation: EHA52574.1 |
RefSeqi | XP_003712381.1, XM_003712333.1 |
Genome annotation databases
EnsemblFungii | MGG_04895T0; MGG_04895T0; MGG_04895 |
GeneIDi | 2675603 |
KEGGi | mgr:MGG_04895 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF540383 Genomic DNA Translation: AAN28719.1 CM001233 Genomic DNA Translation: EHA52574.1 |
RefSeqi | XP_003712381.1, XM_003712333.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5E9F | X-ray | 2.80 | A/B/C/D | 1-547 | [»] | |
5E9G | X-ray | 2.10 | A/B/C/D | 1-547 | [»] | |
SMRi | P0CT06 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 318829.MGG_04895T0 |
Chemistry databases
BindingDBi | P0CT06 |
ChEMBLi | CHEMBL1075264 |
Proteomic databases
PRIDEi | P0CT06 |
Genome annotation databases
EnsemblFungii | MGG_04895T0; MGG_04895T0; MGG_04895 |
GeneIDi | 2675603 |
KEGGi | mgr:MGG_04895 |
Organism-specific databases
VEuPathDBi | FungiDB:MGG_04895 |
Phylogenomic databases
eggNOGi | KOG1260, Eukaryota |
HOGENOMi | CLU_019214_2_2_1 |
OMAi | LEKDWAE |
OrthoDBi | 905115at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00719 |
Miscellaneous databases
PHI-basei | PHI:305 |
PROi | PR:P0CT06 |
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 1 hit |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACEA_MAGO7 | |
Accessioni | P0CT06Primary (citable) accession number: P0CT06 Secondary accession number(s): A4QU17 Q8J232 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 18, 2013 |
Last sequence update: | September 18, 2013 | |
Last modified: | February 10, 2021 | |
This is version 42 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families