UniProtKB - P0CT04 (IPB2_YEAST)
Protein
Protease B inhibitor 2
Gene
PBI2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Cytosolic inhibitor of vacuolar proteinase B (yscB), probably regulating protease B activity during limited proteolysis. PBI2 is a component of the LMA1 complex, which is involved in the facilitation of vesicle fusion such as homotypic vacuole and ER-derived COPII vesicle fusion with the Golgi.5 Publications
Miscellaneous
Originally, 2 inhibitors of protease B, inhibitor I(B)2 and inhibitor I(B)1, have been isolated from commercial baker's yeast, which consists of both S.cerevisiae and S.carlsbergensis. It has been shown that S.cerevisiae only produces inhibitor 2, and S.carlsbergensis only produces inhibitor 1 (PubMed:328499). A sequence for inhibitor 1 from S.carlsbergensis has been determined (AC P0CT05).1 Publication
GO - Molecular functioni
- endopeptidase inhibitor activity Source: SGD
- serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
GO - Biological processi
- negative regulation of endopeptidase activity Source: GO_Central
- protein transport Source: UniProtKB-KW
- regulation of proteolysis Source: SGD
- vacuole fusion, non-autophagic Source: SGD
Keywordsi
Molecular function | Protease inhibitor, Serine protease inhibitor |
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Protease B inhibitor 2Alternative name(s): Proteinase inhibitor I(B)2 |
Gene namesi | Name:PBI2 Ordered Locus Names:YNL015W ORF Names:N2844 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004960, PBI2 |
VEuPathDBi | FungiDB:YNL015W |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000212790 | 2 – 75 | Protease B inhibitor 2Add BLAST | 74 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 74 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P0CT04 |
PRIDEi | P0CT04 |
PTM databases
iPTMneti | P0CT04 |
Interactioni
Subunit structurei
Part of the heterodimeric LMA1 complex together with the thioredoxin II/TRX2. LMA1 binds to the ATPase SEC18.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 35811, 76 interactors |
ComplexPortali | CPX-1278, LMA1 complex, TRX1 variant CPX-1279, LMA1 complex, TRX2 variant |
IntActi | P0CT04, 4 interactors |
STRINGi | 4932.YNL015W |
Miscellaneous databases
RNActi | P0CT04, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502SBW1, Eukaryota |
HOGENOMi | CLU_156026_3_0_1 |
OMAi | DQEMHIL |
Family and domain databases
Gene3Di | 3.30.70.80, 1 hit |
InterProi | View protein in InterPro IPR037045, S8pro/Inhibitor_I9_sf |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0CT04-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKNFIVTLK KNTPDVEAKK FLDSVHHAGG SIVHEFDIIK GYTIKVPDVL
60 70
HLNKLKEKHN DVIENVEEDK EVHTN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 33 | V → L AA sequence (PubMed:387783).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60051 Genomic DNA Translation: CAA42651.1 Z71291 Genomic DNA Translation: CAA95876.1 AY693217 Genomic DNA Translation: AAT93236.1 BK006947 Genomic DNA Translation: DAA10529.1 |
PIRi | S16882, YBBY2 |
RefSeqi | NP_014383.1, NM_001182854.1 |
Genome annotation databases
EnsemblFungii | YNL015W_mRNA; YNL015W; YNL015W |
GeneIDi | 855717 |
KEGGi | sce:YNL015W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60051 Genomic DNA Translation: CAA42651.1 Z71291 Genomic DNA Translation: CAA95876.1 AY693217 Genomic DNA Translation: AAT93236.1 BK006947 Genomic DNA Translation: DAA10529.1 |
PIRi | S16882, YBBY2 |
RefSeqi | NP_014383.1, NM_001182854.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 35811, 76 interactors |
ComplexPortali | CPX-1278, LMA1 complex, TRX1 variant CPX-1279, LMA1 complex, TRX2 variant |
IntActi | P0CT04, 4 interactors |
STRINGi | 4932.YNL015W |
PTM databases
iPTMneti | P0CT04 |
Proteomic databases
PaxDbi | P0CT04 |
PRIDEi | P0CT04 |
Genome annotation databases
EnsemblFungii | YNL015W_mRNA; YNL015W; YNL015W |
GeneIDi | 855717 |
KEGGi | sce:YNL015W |
Organism-specific databases
SGDi | S000004960, PBI2 |
VEuPathDBi | FungiDB:YNL015W |
Phylogenomic databases
eggNOGi | ENOG502SBW1, Eukaryota |
HOGENOMi | CLU_156026_3_0_1 |
OMAi | DQEMHIL |
Miscellaneous databases
PROi | PR:P0CT04 |
RNActi | P0CT04, protein |
Family and domain databases
Gene3Di | 3.30.70.80, 1 hit |
InterProi | View protein in InterPro IPR037045, S8pro/Inhibitor_I9_sf |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IPB2_YEAST | |
Accessioni | P0CT04Primary (citable) accession number: P0CT04 Secondary accession number(s): D6W1G3, P01095 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2013 |
Last sequence update: | June 26, 2013 | |
Last modified: | February 10, 2021 | |
This is version 56 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names