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Protein

Heat shock protein SSC1, mitochondrial

Gene

SSC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Constitutes the ATP-driven core of the motor and binds the precursor preprotein. Required for the import of the processed frataxin homolog YFH1 into the mitochondrion.3 Publications
Acts as a non-catalytic component of endonuclease SceI (Endo.SceI), which cleaves specifically at multiple sites on mitochondrial DNA and produces double-stranded breaks. SSC1 confers broader sequence specificity, greater stability, and higher activity on the catalytic subunit (By similarity).By similarity

Caution

S.cerevisiae displays strain polymorphism with regard to Endo.SceI endouclease activity. This is due to the mitochondrion-encoded, catalytic subunit ENS2, which exhibits strain polymorphism. It can be either present as continuous ORF in the mitochondrial genome (e.g. strain IAM 4274), present but disrupted by the insertion of GC clusters (e.g. strain D273-10B/A), or completely absent in the mitochondrial genome (e.g. strain S288c). Sequences for the 2 subunits ENS2 (AC P12294) and SSC1 (AC P0CS91) for an active Endo.SceI endonuclease can be found in strain IAM 4274.Curated

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • enzyme regulator activity Source: SGD
  • unfolded protein binding Source: InterPro

GO - Biological processi

  • positive regulation of endodeoxyribonuclease activity Source: SGD
  • protein import into mitochondrial matrix Source: SGD
  • protein refolding Source: SGD
  • protein unfolding Source: SGD

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31680-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein SSC1, mitochondrial
Alternative name(s):
Endonuclease SceI 75 kDa subunit
Short name:
Endo.SceI 75 kDa subunit
mtHSP70
Gene namesi
Name:SSC1
Synonyms:ENS1
Ordered Locus Names:YJR045C
ORF Names:J1639
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR045C
SGDiS000003806 SSC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi442P → S: No interaction with TIM44. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23Mitochondrion1 PublicationAdd BLAST23
ChainiPRO_000001355324 – 654Heat shock protein SSC1, mitochondrialAdd BLAST631

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei330PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CS90
PaxDbiP0CS90
PRIDEiP0CS90

PTM databases

iPTMnetiP0CS90

Interactioni

Subunit structurei

Component of endonuclease SceI (Endo.SceI), which is a heterodimer of ENS2 and SSC1 (By similarity). Component of the PAM complex, at least composed of SSC1 (mtHsp70), MGE1, TIM44, PAM16/TIM16, PAM17 and PAM18/TIM14. In the complex, SSC1 interacts directly with PAM18 and TIM44. Interacts with NAP1.By similarity4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33797, 950 interactors
ComplexPortaliCPX-1423 COX1 pre-assembly complex
CPX-1741 Endonuclease SceI
CPX-539 Mitochondrial inner membrane pre-sequence translocase complex
IntActiP0CS90, 401 interactors
STRINGi4932.YJR045C

Structurei

3D structure databases

ProteinModelPortaliP0CS90
SMRiP0CS90
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi634 – 646Asn-richAdd BLAST13

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00920000149123
InParanoidiP0CS90
KOiK04043
OMAiISIKRHM
OrthoDBiEOG092C1VPN

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
HAMAPiMF_00332 DnaK, 1 hit
InterProiView protein in InterPro
IPR012725 Chaperone_DnaK
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
TIGRFAMsiTIGR02350 prok_dnaK, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CS90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAAKNILNR SSLSSSFRIA TRLQSTKVQG SVIGIDLGTT NSAVAIMEGK
60 70 80 90 100
VPKIIENAEG SRTTPSVVAF TKEGERLVGI PAKRQAVVNP ENTLFATKRL
110 120 130 140 150
IGRRFEDAEV QRDIKQVPYK IVKHSNGDAW VEARGQTYSP AQIGGFVLNK
160 170 180 190 200
MKETAEAYLG KPVKNAVVTV PAYFNDSQRQ ATKDAGQIVG LNVLRVVNEP
210 220 230 240 250
TAAALAYGLE KSDSKVVAVF DLGGGTFDIS ILDIDNGVFE VKSTNGDTHL
260 270 280 290 300
GGEDFDIYLL REIVSRFKTE TGIDLENDRM AIQRIREAAE KAKIELSSTV
310 320 330 340 350
STEINLPFIT ADASGPKHIN MKFSRAQFET LTAPLVKRTV DPVKKALKDA
360 370 380 390 400
GLSTSDISEV LLVGGMSRMP KVVETVKSLF GKDPSKAVNP DEAVAIGAAV
410 420 430 440 450
QGAVLSGEVT DVLLLDVTPL SLGIETLGGV FTRLIPRNTT IPTKKSQIFS
460 470 480 490 500
TAAAGQTSVE IRVFQGEREL VRDNKLIGNF TLAGIPPAPK GVPQIEVTFD
510 520 530 540 550
IDADGIINVS ARDKATNKDS SITVAGSSGL SENEIEQMVN DAEKFKSQDE
560 570 580 590 600
ARKQAIETAN KADQLANDTE NSLKEFEGKV DKAEAQKVRD QITSLKELVA
610 620 630 640 650
RVQGGEEVNA EELKTKTEEL QTSSMKLFEQ LYKNDSNNNN NNNGNNAESG

ETKQ
Length:654
Mass (Da):70,628
Last modified:June 13, 2012 - v1
Checksum:i1E5672E76FCE24AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27229 Genomic DNA Translation: AAA63792.1
L36344 Genomic DNA Translation: AAA88747.1
Z49545 Genomic DNA Translation: CAA89573.1
BK006943 Genomic DNA Translation: DAA08832.1
PIRiA32493 HHBYS1
RefSeqiNP_012579.1, NM_001181703.1

Genome annotation databases

EnsemblFungiiYJR045C; YJR045C; YJR045C
GeneIDi853503
KEGGisce:YJR045C

Similar proteinsi

Entry informationi

Entry nameiHSP77_YEAST
AccessioniPrimary (citable) accession number: P0CS90
Secondary accession number(s): D6VWL6, P12398
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 13, 2012
Last modified: July 18, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

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