UniProtKB - P0CK95 (ACFD_ECOLI)
Protein
Putative lipoprotein AcfD homolog
Gene
yghJ
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of folded proteins across the outer membrane.Curated
Miscellaneous
In many other E.coli strains this gene is part of a type II secretion system, but in MG1655 the locus is missing a number of genes.Curated
Enzyme and pathway databases
BioCyci | EcoCyc:G7541-MONOMER |
Protein family/group databases
MEROPSi | M98.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Putative lipoprotein AcfD homolog |
Gene namesi | Name:yghJ Ordered Locus Names:b4466, JW5925 ORF Names:ECK2968 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane PROSITE-ProRule annotation; Lipid-anchor PROSITE-ProRule annotation
GO - Cellular componenti
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | PROSITE-ProRule annotationAdd BLAST | 23 | |
ChainiPRO_0000020619 | 24 – 1520 | Putative lipoprotein AcfD homologAdd BLAST | 1497 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 24 | N-palmitoyl cysteinePROSITE-ProRule annotation | 1 | |
Lipidationi | 24 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
PaxDbi | P0CK95 |
PRIDEi | P0CK95 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262979, 9 interactors |
STRINGi | 511145.b4466 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1081 – 1381 | Peptidase M60PROSITE-ProRule annotationAdd BLAST | 301 |
Sequence similaritiesi
To V.cholerae AcfD (VC_0845).Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3064, Bacteria |
HOGENOMi | CLU_006312_0_0_6 |
Family and domain databases
Gene3Di | 1.10.390.30, 1 hit |
InterProi | View protein in InterPro IPR025385, DUF4092 IPR035423, M60-like_N IPR042279, Pep_M60_3 IPR031161, Peptidase_M60_dom |
Pfami | View protein in Pfam PF13322, DUF4092, 1 hit PF17291, M60-like_N, 1 hit PF13402, Peptidase_M60, 1 hit |
SMARTi | View protein in SMART SM01276, M60-like, 1 hit |
PROSITEi | View protein in PROSITE PS51723, PEPTIDASE_M60, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0CK95-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKKFKYKKS LLAAILSATL LAGCDGGGSG SSSDTPPVDS GTGSLPEVKP
60 70 80 90 100
DPTPNPEPTP EPTPDPEPTP EPIPDPEPTP EPEPEPVPTK TGYLTLGGSQ
110 120 130 140 150
RVTGATCNGE SSDGFTFKPG EDVTCVAGNT TIATFNTQSE AARSLRAVEK
160 170 180 190 200
VSFSLEDAQE LAGSDDKKSN AVSLVTSSNS CPANTEQVCL TFSSVIESKR
210 220 230 240 250
FDSLYKQIDL APEEFKKLVN EEVENNAATD KAPSTHTSPV VPVTTPGTKP
260 270 280 290 300
DLNASFVSAN AEQFYQYQPT EIILSEGRLV DSQGYGVAGV NYYTNSGRGV
310 320 330 340 350
TGENGEFSFS WGETISFGID TFELGSVRGN KSTIALTELG DEVRGANIDQ
360 370 380 390 400
LIHRYSTTGQ NNTRVVPDDV RKVFAEYPNV INEIINLSLS NGATLGEGEQ
410 420 430 440 450
VVNLPNEFIE QFNTGQAKEI DTAICAKTDG CNEARWFSLT TRNVNDGQIQ
460 470 480 490 500
GVINKLWGVD TNYKSVSKFH VFHDSTNFYG STGNARGQAV VNISNAAFPI
510 520 530 540 550
LMARNDKNYW LAFGEKRAWD KNELAYITEA PSLVEPENVT RDTATFNLPF
560 570 580 590 600
ISLGQVGEGK LMVIGNPHYN SILRCPNGYS WNGGVNKDGQ CTLNSDPDDM
610 620 630 640 650
KNFMENVLRY LSDDKWKPDA KASMTVGTNL DTVYFKRHGQ VTGNSAAFDF
660 670 680 690 700
HPDFAGISVE HLSSYGDLDP QEMPLLILNG FEYVTQVGND PYAIPLRADT
710 720 730 740 750
SKPKLTQQDV TDLIAYLNKG GSVLIMENVM SNLKEESASG FVRLLDAAGL
760 770 780 790 800
SMALNKSVVN NDPQGYPNRV RQQRATGIWV YERYPAVDGA LPYTIDSKTG
810 820 830 840 850
EVKWKYQVEN KPDDKPKLEV ASWLEDVDGK QETRYAFIDE ADHKTEDSLK
860 870 880 890 900
AAKEKIFAAF PGLKECTNPA YHYEVNCLEY RPGTGVPVTG GMYVPQYTQL
910 920 930 940 950
SLNADTAKAM VQAADLGTNI QRLYQHELYF RTNGRKGERL SSVDLERLYQ
960 970 980 990 1000
NMSVWLWNDT SYRYEEGKND ELGFKTFTEF LNCYANDAYA GGTKCSADLK
1010 1020 1030 1040 1050
KSLVDNNMIY GDGSSKAGMM NPSYPLNYME KPLTRLMLGR SWWDLNIKVD
1060 1070 1080 1090 1100
VEKYPGAVSE EGQNVTETIS LYSNPTKWFA GNMQSTGLWA PAQKEVTIKS
1110 1120 1130 1140 1150
NANVPVTVTV ALADDLTGRE KHEVALNRPP RVTKTYSLDA SGTVKFKVPY
1160 1170 1180 1190 1200
GGLIYIKGNS STNESASFTF TGVVKAPFYK DGAWKNDLNS PAPLGELESD
1210 1220 1230 1240 1250
AFVYTTPKKN LNASNYTGGL EQFANDLDTF ASSMNDFYGR DSEDGKHRMF
1260 1270 1280 1290 1300
TYKNLPGHKH RFTNDVQISI GDAHSGYPVM NSSFSPNSTT LPTTPLNDWL
1310 1320 1330 1340 1350
IWHEVGHNAA ETPLTVPGAT EVANNVLALY MQDRYLGKMN RVADDITVAP
1360 1370 1380 1390 1400
EYLEESNNQA WARGGAGDRL LMYAQLKEWA EKNFDIKKWY PDGTPLPEFY
1410 1420 1430 1440 1450
SEREGMKGWN LFQLMHRKAR GDEVSNDKFG GKNYCAESNG NAADTLMLCA
1460 1470 1480 1490 1500
SWVAQTDLSE FFKKWNPGAN AYQLPGASEM SFEGGVSQSA YNTLASLDLP
1510 1520
KPEQGPETIN QVTEHKMSAE
Sequence cautioni
The sequence AAA69140 differs from that shown. Reason: Frameshift.Curated
The sequence AAA69141 differs from that shown. Reason: Frameshift.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 1358 | N → G in strain: O15:H- / 83/39 /ETEC. | 1 | |
Natural varianti | 1392 – 1402 | DGTPLPEFYSE → EGELPKFFSD in strain: O15:H- / 83/39 / ETEC. Add BLAST | 11 | |
Natural varianti | 1423 – 1428 | EVSNDK → DVGDKT in strain: O15:H- / 83/39 / ETEC. | 6 | |
Natural varianti | 1498 | D → K in strain: O15:H- / 83/39 /ETEC. | 1 | |
Natural varianti | 1511 | Q → K in strain: O15:H- / 83/39 /ETEC. | 1 | |
Natural varianti | 1519 | A → V in strain: O15:H- / 83/39 /ETEC. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69141.1 Frameshift. U28377 Genomic DNA Translation: AAA69140.1 Frameshift. U00096 Genomic DNA Translation: AAT48156.1 AP009048 Genomic DNA Translation: BAE77034.1 AF426313 Genomic DNA Translation: AAL60194.1 |
RefSeqi | WP_001034469.1, NZ_LN832404.1 YP_026189.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48156; AAT48156; b4466 BAE77034; BAE77034; BAE77034 |
GeneIDi | 2847716 57729208 |
KEGGi | ecj:JW5925 eco:b4466 |
PATRICi | fig|1411691.4.peg.3758 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69141.1 Frameshift. U28377 Genomic DNA Translation: AAA69140.1 Frameshift. U00096 Genomic DNA Translation: AAT48156.1 AP009048 Genomic DNA Translation: BAE77034.1 AF426313 Genomic DNA Translation: AAL60194.1 |
RefSeqi | WP_001034469.1, NZ_LN832404.1 YP_026189.1, NC_000913.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262979, 9 interactors |
STRINGi | 511145.b4466 |
Protein family/group databases
MEROPSi | M98.001 |
Proteomic databases
PaxDbi | P0CK95 |
PRIDEi | P0CK95 |
Genome annotation databases
EnsemblBacteriai | AAT48156; AAT48156; b4466 BAE77034; BAE77034; BAE77034 |
GeneIDi | 2847716 57729208 |
KEGGi | ecj:JW5925 eco:b4466 |
PATRICi | fig|1411691.4.peg.3758 |
Phylogenomic databases
eggNOGi | COG3064, Bacteria |
HOGENOMi | CLU_006312_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7541-MONOMER |
Miscellaneous databases
PROi | PR:P0CK95 |
Family and domain databases
Gene3Di | 1.10.390.30, 1 hit |
InterProi | View protein in InterPro IPR025385, DUF4092 IPR035423, M60-like_N IPR042279, Pep_M60_3 IPR031161, Peptidase_M60_dom |
Pfami | View protein in Pfam PF13322, DUF4092, 1 hit PF17291, M60-like_N, 1 hit PF13402, Peptidase_M60, 1 hit |
SMARTi | View protein in SMART SM01276, M60-like, 1 hit |
PROSITEi | View protein in PROSITE PS51723, PEPTIDASE_M60, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACFD_ECOLI | |
Accessioni | P0CK95Primary (citable) accession number: P0CK95 Secondary accession number(s): Q2M9M2 Q6BF58 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 8, 2011 |
Last sequence update: | March 8, 2011 | |
Last modified: | February 10, 2021 | |
This is version 58 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |