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Protein

Bifunctional ligase/repressor BirA

Gene

birA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor.UniRule annotation

Catalytic activityi

ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118BiotinUniRule annotation1
Binding sitei189BiotinUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi23 – 42H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Ligase, Repressor
Biological processTranscription, Transcription regulation
LigandATP-binding, Biotin, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU22440-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ligase/repressor BirAUniRule annotation
Alternative name(s):
Biotin--[acetyl-CoA-carboxylase] ligaseUniRule annotation (EC:6.3.4.15UniRule annotation)
Biotin--protein ligaseUniRule annotation
Biotin-[acetyl-CoA carboxylase] synthetaseUniRule annotation
Gene namesi
Name:birAUniRule annotation
Ordered Locus Names:BSU22440
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23G → S: Deregulation of biotin synthesis. 1 Publication1
Mutagenesisi38W → R: Deregulation of biotin synthesis. 1 Publication1
Mutagenesisi58G → E: Deregulation of biotin synthesis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000649311 – 325Bifunctional ligase/repressor BirAAdd BLAST325

Proteomic databases

PaxDbiP0CI75
PRIDEiP0CI75

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012336

Structurei

3D structure databases

ProteinModelPortaliP0CI75
SMRiP0CI75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 262BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 124Biotin bindingUniRule annotation3

Sequence similaritiesi

Belongs to the biotin--protein ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105HJX Bacteria
COG0340 LUCA
COG1654 LUCA
HOGENOMiHOG000041811
InParanoidiP0CI75
KOiK03524
OMAiRAAVWKH
PhylomeDBiP0CI75

Family and domain databases

CDDicd16442 BPL, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00978 Bifunct_BirA, 1 hit
InterProiView protein in InterPro
IPR030855 Bifunct_BirA
IPR004408 Biotin_CoA_COase_ligase
IPR004409 Biotin_operon_repress_HTH
IPR003142 BPL_C
IPR004143 BPL_LPL_catalytic
IPR013196 HTH_11
IPR008988 Transcriptional_repressor_C
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12835:SF5 PTHR12835:SF5, 1 hit
PfamiView protein in Pfam
PF02237 BPL_C, 1 hit
PF03099 BPL_LplA_LipB, 1 hit
PF08279 HTH_11, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50037 SSF50037, 1 hit
TIGRFAMsiTIGR00121 birA_ligase, 1 hit
TIGR00122 birA_repr_reg, 1 hit
PROSITEiView protein in PROSITE
PS51733 BPL_LPL_CATALYTIC, 1 hit

Sequencei

Sequence statusi: Complete.

P0CI75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSTLRKDLI ELFSQAGNEF ISGQKISDAL GCSRTAVWKH IEELRKEGYE
60 70 80 90 100
VEAVRRKGYR LIKKPGKLSE SEIRFGLKTE VMGQHLIYHD VLSSTQKTAH
110 120 130 140 150
ELANNNAPEG TLVVADKQTA GRGRMSRVWH SQEGNGVWMS LILRPDIPLQ
160 170 180 190 200
KTPQLTLLAA VAVVQGIEEA AGIQTDIKWP NDILINGKKT VGILTEMQAE
210 220 230 240 250
EDRVRSVIIG IGINVNQQPN DFPDELKDIA TSLSQAAGEK IDRAGVIQHI
260 270 280 290 300
LLCFEKRYRD YMTHGFTPIK LLWESYALGI GTNMRARTLN GTFYGKALGI
310 320
DDEGVLLLET NEGIKKIYSA DIELG
Length:325
Mass (Da):36,182
Last modified:January 11, 2011 - v1
Checksum:i69088BB2CAD09221
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38424 Genomic DNA Translation: AAA92879.1 Sequence problems.
L47709 Genomic DNA Translation: AAB38447.1
AL009126 Genomic DNA Translation: CAB14160.1
PIRiA69595
RefSeqiNP_390125.1, NC_000964.3
WP_003230650.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14160; CAB14160; BSU22440
GeneIDi939028
KEGGibsu:BSU22440
PATRICifig|224308.179.peg.2448

Similar proteinsi

Entry informationi

Entry nameiBIRA_BACSU
AccessioniPrimary (citable) accession number: P0CI75
Secondary accession number(s): P42975
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 11, 2011
Last modified: June 20, 2018
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

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