UniProtKB - P0CG08 (GPHRB_HUMAN)
Protein
Golgi pH regulator B
Gene
GPR89B
Organism
Homo sapiens (Human)
Status
Functioni
Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance (PubMed:18794847). Plays a role in lymphocyte development, probably by acting as a RABL3 effector in hematopoietic cells (By similarity).By similarity1 Publication
Miscellaneous
Does not seem to be able to bind GTP.
GO - Molecular functioni
- voltage-gated anion channel activity Source: GO_Central
GO - Biological processi
- intracellular pH reduction Source: GO_Central
- protein transport Source: UniProtKB-KW
- regulation of ion transmembrane transport Source: UniProtKB-KW
- T cell differentiation Source: UniProtKB
Keywordsi
Molecular function | Ion channel, Voltage-gated channel |
Biological process | Ion transport, Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | P0CG08 |
Names & Taxonomyi
Protein namesi | Recommended name: Golgi pH regulator BAlternative name(s): Protein GPR89B |
Gene namesi | Name:GPR89B Synonyms:GPHRB, GPR89C ORF Names:HSPC201 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:13840, GPR89B |
MIMi | 612806, gene |
neXtProti | NX_P0CG08 |
VEuPathDBi | HostDB:ENSG00000188092.14 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein Sequence analysis
Golgi apparatus
- Golgi cisterna membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 5 – 25 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 46 – 66 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 79 – 99 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 114 – 134 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 150 – 170 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 290 – 310 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 343 – 363 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 378 – 398 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 425 – 445 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
PharmGKBi | PA134986137 |
Miscellaneous databases
Pharosi | P0CG08, Tdark |
Genetic variation databases
BioMutai | GPR89B |
DMDMi | 298351693 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000395006 | 1 – 455 | Golgi pH regulator BAdd BLAST | 455 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 180 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 243 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | P0CG08 |
jPOSTi | P0CG08 |
PeptideAtlasi | P0CG08 |
PRIDEi | P0CG08 |
ProteomicsDBi | 1413 52451 |
PTM databases
GlyGeni | P0CG08, 2 sites |
iPTMneti | P0CG08 |
PhosphoSitePlusi | P0CG08 |
SwissPalmi | P0CG08 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000188092, Expressed in adult mammalian kidney and 114 other tissues |
ExpressionAtlasi | P0CG08, baseline and differential |
Genevisiblei | P0CG08, HS |
Organism-specific databases
HPAi | ENSG00000188092, Low tissue specificity |
Interactioni
Subunit structurei
Homotrimer (PubMed:18794847).
Interacts with RABL3; the interaction stabilizes GPR89B (By similarity).
By similarity1 PublicationBinary interactionsi
P0CG08
With | #Exp. | IntAct |
---|---|---|
WFS1 [O76024] | 3 | EBI-11905631,EBI-720609 |
Protein-protein interaction databases
BioGRIDi | 119554, 26 interactors 575849, 75 interactors |
IntActi | P0CG08, 15 interactors |
Miscellaneous databases
RNActi | P0CG08, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00390000000684 |
HOGENOMi | CLU_030540_1_0_1 |
InParanoidi | P0CG08 |
OMAi | HKVQSFA |
OrthoDBi | 1065554at2759 |
PhylomeDBi | P0CG08 |
TreeFami | TF313484 |
Family and domain databases
InterProi | View protein in InterPro IPR025969, ABA_GPCR_dom IPR022535, Golgi_pH-regulator_cons_dom IPR015672, GPHR/GTG |
PANTHERi | PTHR15948, PTHR15948, 1 hit |
Pfami | View protein in Pfam PF12430, ABA_GPCR, 1 hit PF12537, GPHR_N, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P0CG08-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFLIDSSIM ITSQILFFGF GWLFFMRQLF KDYEIRQYVV QVIFSVTFAF
60 70 80 90 100
SCTMFELIIF EILGVLNSSS RYFHWKMNLC VILLILVFMV PFYIGYFIVS
110 120 130 140 150
NIRLLHKQRL LFSCLLWLTF MYFFWKLGDP FPILSPKHGI LSIEQLISRV
160 170 180 190 200
GVIGVTLMAL LSGFGAVNCP YTYMSYFLRN VTDTDILALE RRLLQTMDMI
210 220 230 240 250
ISKKKRMAMA RRTMFQKGEV HNKPSGFWGM IKSVTTSASG SENLTLIQQE
260 270 280 290 300
VDALEELSRQ LFLETADLYA TKERIEYSKT FKGKYFNFLG YFFSIYCVWK
310 320 330 340 350
IFMATINIVF DRVGKTDPVT RGIEITVNYL GIQFDVKFWS QHISFILVGI
360 370 380 390 400
IIVTSIRGLL ITLTKFFYAI SSSKSSNVIV LLLAQIMGMY FVSSVLLIRM
410 420 430 440 450
SMPLEYRTII TEVLGELQFN FYHRWFDVIF LVSALSSILF LYLAHKQAPE
KQMAP
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketU3KPV2 | U3KPV2_HUMAN | Golgi pH regulator B | GPR89B | 216 | Annotation score: | ||
A0A087WW82 | A0A087WW82_HUMAN | Golgi pH regulator B | GPR89B | 128 | Annotation score: | ||
A0A087WW78 | A0A087WW78_HUMAN | Golgi pH regulator B | GPR89B | 93 | Annotation score: |
Sequence cautioni
The sequence AAF36121 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 244 – 247 | LTLI → NTMA in CAH56393 (PubMed:17974005).Curated | 4 | |
Sequence conflicti | 251 | V → E in AAF36121 (PubMed:11042152).Curated | 1 | |
Sequence conflicti | 418 | Q → R in AAF36121 (PubMed:11042152).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U78723 mRNA Translation: AAF21463.1 AL356004 Genomic DNA Translation: CAI17085.1 AL445591, BX537254 Genomic DNA Translation: CAH72386.1 BX537254, AL445591 Genomic DNA Translation: CAI18821.1 AL133020 mRNA Translation: CAH56393.1 AF151035 mRNA Translation: AAF36121.1 Sequence problems. |
CCDSi | CCDS930.1 |
RefSeqi | NP_001091081.1, NM_001097612.1 NP_057418.1, NM_016334.3 XP_005277458.1, XM_005277401.3 XP_006711555.1, XM_006711492.3 XP_011507915.1, XM_011509613.2 XP_011508212.1, XM_011509910.2 |
Genome annotation databases
Ensembli | ENST00000314163; ENSP00000358233; ENSG00000188092 |
GeneIDi | 51463 653519 |
KEGGi | hsa:51463 hsa:653519 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U78723 mRNA Translation: AAF21463.1 AL356004 Genomic DNA Translation: CAI17085.1 AL445591, BX537254 Genomic DNA Translation: CAH72386.1 BX537254, AL445591 Genomic DNA Translation: CAI18821.1 AL133020 mRNA Translation: CAH56393.1 AF151035 mRNA Translation: AAF36121.1 Sequence problems. |
CCDSi | CCDS930.1 |
RefSeqi | NP_001091081.1, NM_001097612.1 NP_057418.1, NM_016334.3 XP_005277458.1, XM_005277401.3 XP_006711555.1, XM_006711492.3 XP_011507915.1, XM_011509613.2 XP_011508212.1, XM_011509910.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 119554, 26 interactors 575849, 75 interactors |
IntActi | P0CG08, 15 interactors |
PTM databases
GlyGeni | P0CG08, 2 sites |
iPTMneti | P0CG08 |
PhosphoSitePlusi | P0CG08 |
SwissPalmi | P0CG08 |
Genetic variation databases
BioMutai | GPR89B |
DMDMi | 298351693 |
Proteomic databases
EPDi | P0CG08 |
jPOSTi | P0CG08 |
PeptideAtlasi | P0CG08 |
PRIDEi | P0CG08 |
ProteomicsDBi | 1413 52451 |
Protocols and materials databases
Antibodypediai | 65496, 54 antibodies |
DNASUi | 51463 |
Genome annotation databases
Ensembli | ENST00000314163; ENSP00000358233; ENSG00000188092 |
GeneIDi | 51463 653519 |
KEGGi | hsa:51463 hsa:653519 |
Organism-specific databases
CTDi | 51463 653519 |
GeneCardsi | GPR89B |
HGNCi | HGNC:13840, GPR89B |
HPAi | ENSG00000188092, Low tissue specificity |
MIMi | 612806, gene |
neXtProti | NX_P0CG08 |
PharmGKBi | PA134986137 |
VEuPathDBi | HostDB:ENSG00000188092.14 |
GenAtlasi | Search... |
Phylogenomic databases
GeneTreei | ENSGT00390000000684 |
HOGENOMi | CLU_030540_1_0_1 |
InParanoidi | P0CG08 |
OMAi | HKVQSFA |
OrthoDBi | 1065554at2759 |
PhylomeDBi | P0CG08 |
TreeFami | TF313484 |
Enzyme and pathway databases
PathwayCommonsi | P0CG08 |
Miscellaneous databases
BioGRID-ORCSi | 51463, 87 hits in 551 CRISPR screens 653519, 238 hits in 907 CRISPR screens |
Pharosi | P0CG08, Tdark |
PROi | PR:P0CG08 |
RNActi | P0CG08, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000188092, Expressed in adult mammalian kidney and 114 other tissues |
ExpressionAtlasi | P0CG08, baseline and differential |
Genevisiblei | P0CG08, HS |
Family and domain databases
InterProi | View protein in InterPro IPR025969, ABA_GPCR_dom IPR022535, Golgi_pH-regulator_cons_dom IPR015672, GPHR/GTG |
PANTHERi | PTHR15948, PTHR15948, 1 hit |
Pfami | View protein in Pfam PF12430, ABA_GPCR, 1 hit PF12537, GPHR_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPHRB_HUMAN | |
Accessioni | P0CG08Primary (citable) accession number: P0CG08 Secondary accession number(s): A6NKF9 Q9Y302 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 15, 2010 |
Last sequence update: | June 15, 2010 | |
Last modified: | April 7, 2021 | |
This is version 77 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families