UniProtKB - P0CE41 (HAP1_YEAST)
Protein
Heme-responsive zinc finger transcription factor HAP1
Gene
HAP1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Regulation of oxygen dependent gene expression. It modulates the expression of Iso-1 (CYP1) and Iso-2 (CYP3) cytochrome c. In response to heme, promotes transcription of genes encoding functions required for respiration, controlling oxidative damage and repression of anaerobic genes. Binds to the sequence 5'-CGGNNNTNNCGG-3' (By similarity). Is non-functional in terms of iso-1 cytochrome c expression in strain S288c and its derivatives.By similarity1 Publication
Miscellaneous
Heme is an effector molecule for CYP1/HAP1. The HRM repeat region mediates heme induction by masking the DNA-binding domain in the absence of inducer (By similarity).By similarity
The sequence for strain S288c and its derivatives differs from other strain backgrounds due to the insertion of a defective Ty1 element in the C-terminus. This acts as a null allele in terms of iso-1 cytochrome c expression and beta-galactosidase activity. Also, the expression levels of ergosterol-related genes and ergosterol content are decreased in laboratory strain X2180 caused by the defective Ty1 insertion. The sequence of a wild-type allele can be found in other strain backgrounds (AC P0CS82).
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Zinc 1By similarity | 1 | |
Metal bindingi | 64 | Zinc 2By similarity | 1 | |
Metal bindingi | 67 | Zinc 1By similarity | 1 | |
Metal bindingi | 74 | Zinc 1By similarity | 1 | |
Metal bindingi | 81 | Zinc 1By similarity | 1 | |
Metal bindingi | 81 | Zinc 2By similarity | 1 | |
Metal bindingi | 84 | Zinc 2By similarity | 1 | |
Metal bindingi | 93 | Zinc 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 64 – 93 | Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST | 30 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: SGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: SGD
- sequence-specific DNA binding Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- carbon catabolite activation of transcription from RNA polymerase II promoter Source: SGD
- establishment of protein localization to chromatin Source: SGD
- negative regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: SGD
- regulation of cellular respiration Source: SGD
- regulation of transcription by RNA polymerase II Source: GO_Central
- transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Heme, Iron, Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Heme-responsive zinc finger transcription factor HAP1Alternative name(s): CYP1 activatory protein Heme activator protein 1 |
Gene namesi | Name:HAP1 Synonyms:CYP1 Ordered Locus Names:YLR256W ORF Names:L9672.1 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004246, HAP1 |
VEuPathDBi | FungiDB:YLR256W |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000114945 | 1 – 1502 | Heme-responsive zinc finger transcription factor HAP1Add BLAST | 1502 |
Proteomic databases
MaxQBi | P0CE41 |
PaxDbi | P0CE41 |
PRIDEi | P0CE41 |
PTM databases
iPTMneti | P0CE41 |
Interactioni
Subunit structurei
Binds DNA as a homodimer.
Interacts with SRO9 and YDJ1. In the absence of heme, binds to at least four cellular proteins, including YDJ1 and SRO9, forming a high-molecular-weight complex (HMC) which results in repression of its activity and dictates its DNA-binding specificity (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 31522, 72 interactors |
ComplexPortali | CPX-1276, HMC complex CPX-1882, HAP1 transcriptional repressor complex, SSA1 variant CPX-1883, HAP1 transcriptional repressor complex, SSA2 variant |
IntActi | P0CE41, 4 interactors |
MINTi | P0CE41 |
STRINGi | 4932.YLR256W |
Miscellaneous databases
RNActi | P0CE41, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 280 – 285 | HRM 1 | 6 | |
Repeati | 299 – 304 | HRM 2 | 6 | |
Repeati | 323 – 328 | HRM 3 | 6 | |
Repeati | 347 – 352 | HRM 4 | 6 | |
Repeati | 389 – 394 | HRM 5 | 6 | |
Repeati | 415 – 420 | HRM 6 | 6 | |
Repeati | 1192 – 1197 | HRM 7 | 6 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 244 – 444 | Heme-responsive; required for HMC formationBy similarityAdd BLAST | 201 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 105 – 134 | Sequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 177 – 189 | Poly-GlnAdd BLAST | 13 |
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | ENOG502QRPQ, Eukaryota |
HOGENOMi | CLU_004380_0_0_1 |
InParanoidi | P0CE41 |
OMAi | YMEQTWA |
Family and domain databases
CDDi | cd00067, GAL4, 1 hit |
Gene3Di | 4.10.240.10, 1 hit |
InterProi | View protein in InterPro IPR007219, Transcription_factor_dom_fun IPR001138, Zn2-C6_fun-type_DNA-bd IPR036864, Zn2-C6_fun-type_DNA-bd_sf |
Pfami | View protein in Pfam PF00172, Zn_clus, 1 hit |
SMARTi | View protein in SMART SM00906, Fungal_trans, 1 hit SM00066, GAL4, 1 hit |
SUPFAMi | SSF57701, SSF57701, 1 hit |
PROSITEi | View protein in PROSITE PS00463, ZN2_CY6_FUNGAL_1, 1 hit PS50048, ZN2_CY6_FUNGAL_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0CE41-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNTPYNSSV PSIASMTQSS VSRSPNMHTA TTPGANTSSN SPPLHMSSDS
60 70 80 90 100
SKIKRKRNRI PLSCTICRKR KVKCDKLRPH CQQCTKTGVA HLCHYMEQTW
110 120 130 140 150
AEEAEKELLK DNELKKLRER VKSLEKTLSK VHSSPSSNSL KSYNTPESSN
160 170 180 190 200
LFMGSDEHTT LVNANTGSAS SASHMHQQQQ QQQQQEQQQD FSRSANANAN
210 220 230 240 250
SSSLSISNKY DNDELDLTKD FDLLHIKSNG TIHLGATHWL SIMKGDPYLK
260 270 280 290 300
LLWGHIFAMR EKLNEWYYQK NSYSKLKSSK CPINHAQAPP SAAAAATRKC
310 320 330 340 350
PVDHSAFSSG MVAPKEETPL PRKCPVDHTM FSSGMIPPRE DTSSQKRCPV
360 370 380 390 400
DHTMYSAGMM PPKDETPSPF STKAMIDHNK HTMNPPQSKC PVDHRNYMKD
410 420 430 440 450
YPSDMANSSS NPASRCPIDH SSMKNTAALP ASTHNTIPHH QPQSGSHARS
460 470 480 490 500
HPAQSRKHDS YMTESEVLAT LCEMLPPKRV IALFIEKFFK HLYPAIPILD
510 520 530 540 550
EQNFKNHVNQ MLSLSSMNPT VNNFGMSMPS SSTLENQPIT QINLPKLSDS
560 570 580 590 600
CNLGILIIIL RLTWLSIPSN SCEVDLGEES GSFLVPNESS NMSASALTSM
610 620 630 640 650
AKEESLLLKH ETPVEALELC QKYLIKFDEL SSISNNNVNL TTVQFAIFYN
660 670 680 690 700
FYMKSASNDL TTLTNTNNTG MANPGHDSES HQILLSNITQ MAFSCGLHRD
710 720 730 740 750
PDNFPQLNAT IPATSQDVSN NGSKKANPST NPTLNNNMSA ATTNSSSRSG
760 770 780 790 800
SADSRSGSNP VNKKENQVSI ERFKHTWRKI WYYIVSMDVN QSLSLGSPRL
810 820 830 840 850
LRNLRDFSDT KLPSASRIDY VRDIKELIIV KNFTLFFQID LCIIAVLNHI
860 870 880 890 900
LNVSLARSVR KFELDSLINL LKNLTYGTEN VNDVVSSLIN KGLLPTSEGG
910 920 930 940 950
SVDSNNDEIY GLPKLPDILN HGQHNQNLYA DGRNTSSSDI DKKLDLPHES
960 970 980 990 1000
TTRALFFSKH MTIRMLLYLL NYILFTHYEP MGSEDPGTNI LAKEYAQEAL
1010 1020 1030 1040 1050
NFAMDGYRNC MIFFNNIRNT NSLFDYMNVI LSYPCLDIGH RSLQFIVCLI
1060 1070 1080 1090 1100
LRAKCGPLTG MRESSIITNG TSSGFNSSVE DEDVKVKQES SDELKKDDFM
1110 1120 1130 1140 1150
KDVNLDSGDS LAEILMSRML LFQKLTKQLS KKYNYAIRMN KSTGFFVSLL
1160 1170 1180 1190 1200
DTPSKKSDSK SGGSSFMLGN WKHPKVSNMS GFLAGDKDQL QKCPVYQDAL
1210 1220 1230 1240 1250
GFVSPTGANE GSAPMQGMSL QGSTARMGGT QLPPIRSYKP ITYTSSNLRR
1260 1270 1280 1290 1300
MNETGEAEAK RRRFNDGYID NNSNNDIPRG ISPKPSNGLS SVQPLLSSFS
1310 1320 1330 1340 1350
MNQLNGGTIP TVPSLTNITS QMGALPSLDR ITTNQINLPD PSRDEAFDNS
1360 1370 1380 1390 1400
IKQMTPMTSA FMNANTTIPS STLNGNMNMN GAGTANTDTS ANGSALSTLT
1410 1420 1430 1440 1450
SPQGSDLASN SATQYKPDLE DFLMQNSNFN GLMINPSSLV EVVGGYNDPN
1460 1470 1480 1490 1500
NLGRNDAVDF LPVDNVEIDG VGIKINYHLL TSIYVTSILS YTVLEDDAND
EK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U20865 Genomic DNA Translation: AAB67387.1 BK006945 Genomic DNA Translation: DAA09568.1 |
PIRi | S59400, RGBYH1 |
RefSeqi | NP_013357.1, NM_001182143.1 |
Genome annotation databases
EnsemblFungii | YLR256W_mRNA; YLR256W; YLR256W |
GeneIDi | 850958 |
KEGGi | sce:YLR256W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U20865 Genomic DNA Translation: AAB67387.1 BK006945 Genomic DNA Translation: DAA09568.1 |
PIRi | S59400, RGBYH1 |
RefSeqi | NP_013357.1, NM_001182143.1 |
3D structure databases
SMRi | P0CE41 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31522, 72 interactors |
ComplexPortali | CPX-1276, HMC complex CPX-1882, HAP1 transcriptional repressor complex, SSA1 variant CPX-1883, HAP1 transcriptional repressor complex, SSA2 variant |
IntActi | P0CE41, 4 interactors |
MINTi | P0CE41 |
STRINGi | 4932.YLR256W |
PTM databases
iPTMneti | P0CE41 |
Proteomic databases
MaxQBi | P0CE41 |
PaxDbi | P0CE41 |
PRIDEi | P0CE41 |
Genome annotation databases
EnsemblFungii | YLR256W_mRNA; YLR256W; YLR256W |
GeneIDi | 850958 |
KEGGi | sce:YLR256W |
Organism-specific databases
SGDi | S000004246, HAP1 |
VEuPathDBi | FungiDB:YLR256W |
Phylogenomic databases
eggNOGi | ENOG502QRPQ, Eukaryota |
HOGENOMi | CLU_004380_0_0_1 |
InParanoidi | P0CE41 |
OMAi | YMEQTWA |
Miscellaneous databases
ChiTaRSi | HAP1, yeast |
PROi | PR:P0CE41 |
RNActi | P0CE41, protein |
Family and domain databases
CDDi | cd00067, GAL4, 1 hit |
Gene3Di | 4.10.240.10, 1 hit |
InterProi | View protein in InterPro IPR007219, Transcription_factor_dom_fun IPR001138, Zn2-C6_fun-type_DNA-bd IPR036864, Zn2-C6_fun-type_DNA-bd_sf |
Pfami | View protein in Pfam PF00172, Zn_clus, 1 hit |
SMARTi | View protein in SMART SM00906, Fungal_trans, 1 hit SM00066, GAL4, 1 hit |
SUPFAMi | SSF57701, SSF57701, 1 hit |
PROSITEi | View protein in PROSITE PS00463, ZN2_CY6_FUNGAL_1, 1 hit PS50048, ZN2_CY6_FUNGAL_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HAP1_YEAST | |
Accessioni | P0CE41Primary (citable) accession number: P0CE41 Secondary accession number(s): D6VYQ2 Q6BD21 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 2, 2010 |
Last sequence update: | March 2, 2010 | |
Last modified: | February 10, 2021 | |
This is version 81 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names