NDUFS1

Functionⁱ

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityⁱ

NADH + ubiquinone + 5 H⁺(In) = NAD⁺ + ubiquinol + 4 H⁺(Out).

NADH + acceptor = NAD⁺ + reduced acceptor.

Cofactorⁱ

Protein has several cofactor binding sites:

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	64	Iron-sulfur 1 (2Fe-2S)By similarity	1
Metal bindingⁱ	75	Iron-sulfur 1 (2Fe-2S)By similarity	1
Metal bindingⁱ	78	Iron-sulfur 1 (2Fe-2S)By similarity	1
Metal bindingⁱ	92	Iron-sulfur 1 (2Fe-2S)By similarity	1
Metal bindingⁱ	124	Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation	1
Metal bindingⁱ	128	Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation	1
Metal bindingⁱ	131	Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation	1
Metal bindingⁱ	137	Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation	1
Metal bindingⁱ	176	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	179	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	182	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	226	Iron-sulfur 3 (4Fe-4S)By similarity	1

GO - Molecular functionⁱ

2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
electron transfer activity Source: InterPro
metal ion binding Source: UniProtKB-KW
NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic mitochondrial changes Source: UniProtKB
ATP metabolic process Source: UniProtKB
ATP synthesis coupled electron transport Source: InterPro
cellular respiration Source: UniProtKB
reactive oxygen species metabolic process Source: UniProtKB
regulation of mitochondrial membrane potential Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Electron transport, Respiratory chain, Transport
Ligand	2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3) Alternative name(s): Complex I-75kD Short name: CI-75kD
Gene namesⁱ	Name:NDUFS1
Organismⁱ	Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifierⁱ	9601 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo
Proteomesⁱ	UP000001595 Componentⁱ: Unplaced

Keywords - Cellular componentⁱ

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transit peptideⁱ	1 – 23	MitochondrionBy similarityAdd BLAST		23
ChainⁱPRO_0000019970	24 – 727	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialAdd BLAST		704

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	84	N6-acetyllysineBy similarity	1
Modified residueⁱ	467	N6-acetyllysineBy similarity	1
Modified residueⁱ	499	N6-acetyllysineBy similarity	1
Modified residueⁱ	709	N6-acetyllysineBy similarity	1

Keywords - PTMⁱ

Acetylation

Proteomic databases

PRIDEⁱ	P0CB67

PRIDEⁱ

P0CB67

Interactionⁱ

Subunit structureⁱ

Complex I is composed of 45 different subunits.By similarity

Protein-protein interaction databases

STRINGⁱ	9601.ENSPPYP00000014646

STRINGⁱ

9601.ENSPPYP00000014646

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0CB67
SMRⁱ	P0CB67
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	30 – 108	2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST	79
Domainⁱ	108 – 147	4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST	40
Domainⁱ	245 – 301	4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST	57

Sequence similaritiesⁱ

Belongs to the complex I 75 kDa subunit family.Curated

Keywords - Domainⁱ

Transit peptide

Phylogenomic databases

eggNOGⁱ	KOG2282 Eukaryota COG1034 LUCA
InParanoidⁱ	P0CB67
KEGG Orthology (KO) More...KOⁱ	K03934

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00207 fer2, 1 hit
InterProⁱ	View protein in InterPro IPR036010 2Fe-2S_ferredoxin-like_sf IPR001041 2Fe-2S_ferredoxin-type IPR006656 Mopterin_OxRdtase IPR006963 Mopterin_OxRdtase_4Fe-4S_dom IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228 NADH_UbQ_OxRdtase_Gsu IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd IPR015405 NuoG_C
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00384 Molybdopterin, 1 hit PF10588 NADH-G_4Fe-4S_3, 1 hit PF09326 NADH_dhqG_C, 1 hit
SMARTⁱ	View protein in SMART SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMⁱ	SSF54292 SSF54292, 1 hit
TIGRFAMsⁱ	TIGR01973 NuoG, 1 hit
PROSITEⁱ	View protein in PROSITE PS51085 2FE2S_FER_2, 1 hit PS51839 4FE4S_HC3, 1 hit PS51669 4FE4S_MOW_BIS_MGD, 1 hit PS00641 COMPLEX1_75K_1, 1 hit PS00642 COMPLEX1_75K_2, 1 hit PS00643 COMPLEX1_75K_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P0CB67-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLRIPVRKAL VGLSKSPKGC VRTTATAASN LIEVFVDGQS VMVEPGTTVL 
        60         70         80         90        100
QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW 
       110        120        130        140        150
NILTNSEKSK KAREGVMELL LANHPLDCPI CDQGGECDLQ DQSMMFGNDR 
       160        170        180        190        200
SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT RCIRFASEIA GVDDLGTTGR 
       210        220        230        240        250
GNDMQVGTYI EKMFMSELSG NIIDICPVGA LTSKPYAFTA RPWETRKTES 
       260        270        280        290        300
IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ 
       310        320        330        340        350
RLTEPMVRNE KGLLTYTSWE DALSRVAGML QSFQGKDVAA IAGGLVDAEA 
       360        370        380        390        400
LVALKDLLNR VDSDTLCTEE VFPTAGAGTD LRSNYLLNTT IAGVEEADVV 
       410        420        430        440        450
LLVGTNPRFE APLFNARIRK SWLHNDLKVA LIGSPVDLTY TYDHLGDSPK 
       460        470        480        490        500
ILQDIASGSH PFSQVLKEAK KPMVVLGSSA LQRNDGAAIL AAFSSIAQKI 
       510        520        530        540        550
RMTSGVTGDW KVMNILHRIA SQVAALDLGY KPGVEAIRKN PPRLLFLLGA 
       560        570        580        590        600
DGGCITRQDL PKDCFIIYQG HHGDVGAPIA DVILPGAAYT EKSATYVNTE 
       610        620        630        640        650
GRAQQTKVAV TPPGLAREDW KIIRALSEIA GVTLPYDTLD QVRNRLEEVS 
       660        670        680        690        700
PNLVRYDDIE GANYFQQANE LSKLVNQQLL ADPLVPPQLT IKDFYMTDSI 
       710        720 
SRASQTMAKC VKAVTEGAQA VEEPSIC

Length:727

Mass (Da):79,492

Last modified:November 24, 2009 - v1

Checksum:ⁱF6B9C83DD7A8CC3C

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CR859585 mRNA Translation: CAH91749.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001126016.1, NM_001132544.1

Genome annotation databases

GeneIDⁱ	100172962
KEGGⁱ	pon:100172962

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P0CB67	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial	)	HUMAN		727
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial		GORGO		727
UPI0003F1514B		CHRAS		747
UPI0002ADA9A6		NOMLE		745
NADH:ubiquinone oxidoreductase core subunit S1		MYOLU		744
+230

Protein	Similar proteins		Species	Score	Length
P0CB67	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial	)	HUMAN		727
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial		GORGO		727
UPI0003F1514B		CHRAS		747
UPI0002ADA9A6		NOMLE		745
NADH:ubiquinone oxidoreductase core subunit S1		MYOLU		744
+761

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CR859585 mRNA Translation: CAH91749.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001126016.1, NM_001132544.1

3D structure databases

ProteinModelPortalⁱ	P0CB67
SMRⁱ	P0CB67
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	9601.ENSPPYP00000014646

STRINGⁱ

9601.ENSPPYP00000014646

Proteomic databases

PRIDEⁱ	P0CB67

PRIDEⁱ

P0CB67

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	100172962
KEGGⁱ	pon:100172962

Organism-specific databases

CTDⁱ	4719

CTDⁱ

4719

Phylogenomic databases

eggNOGⁱ	KOG2282 Eukaryota COG1034 LUCA
InParanoidⁱ	P0CB67
KEGG Orthology (KO) More...KOⁱ	K03934

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00207 fer2, 1 hit
InterProⁱ	View protein in InterPro IPR036010 2Fe-2S_ferredoxin-like_sf IPR001041 2Fe-2S_ferredoxin-type IPR006656 Mopterin_OxRdtase IPR006963 Mopterin_OxRdtase_4Fe-4S_dom IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228 NADH_UbQ_OxRdtase_Gsu IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd IPR015405 NuoG_C
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00384 Molybdopterin, 1 hit PF10588 NADH-G_4Fe-4S_3, 1 hit PF09326 NADH_dhqG_C, 1 hit
SMARTⁱ	View protein in SMART SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMⁱ	SSF54292 SSF54292, 1 hit
TIGRFAMsⁱ	TIGR01973 NuoG, 1 hit
PROSITEⁱ	View protein in PROSITE PS51085 2FE2S_FER_2, 1 hit PS51839 4FE4S_HC3, 1 hit PS51669 4FE4S_MOW_BIS_MGD, 1 hit PS00641 COMPLEX1_75K_1, 1 hit PS00642 COMPLEX1_75K_2, 1 hit PS00643 COMPLEX1_75K_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NDUS1_PONAB
Accessionⁱ	P0CB67Primary (citable) accession number: P0CB67 Secondary accession number(s): Q0MQG0, Q5R911
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
	Last sequence update:	November 24, 2009
	Last modified:	February 28, 2018
	This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

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UniProtKB - P0CB67 (NDUS1_PONAB)

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NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

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<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Mitochondrion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

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3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ