UniProtKB - P0CAS0 (PA2A2_CERCE)
Acidic phospholipase A2 CC-PLA2-2
Functioni
Snake venom phospholipase A2 that inhibits blood coagulation and platelet aggregation induced by ADP and arachidonic acid. Inhibits tumor cell adhesion and migration in a dose-dependent manner. Abolishes the attachment of human brain microvascular endothelial cells (HBMEC) to fibrinogen (IC50=0.2 µM) and dramatically reduces it adhesion to fibronectin (IC50=0.3 µM), whereas no effect is observed on type I collagen, vitronectin or laminin 1. Also blocks the cell migration toward fibronectin and fibrinogen. These effects are not dependent of the catalytic activity, but are mediated by alpha-5/beta-1 (ITGA5/ITGB1) and alpha-v-containing (ITGAV) integrins. Also shows anti-angiogenic activity in chicken chorioallantoix membrane assay. Has a relatively high enzymatic activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
2 PublicationsMiscellaneous
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+PROSITE-ProRule annotationEC:3.1.1.4PROSITE-ProRule annotation
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 45 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 47 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 63 | By similarity | 1 | |
Metal bindingi | 64 | CalciumBy similarity | 1 | |
Active sitei | 103 | By similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- angiogenesis Source: UniProtKB-KW
- arachidonic acid secretion Source: InterPro
- lipid catabolic process Source: UniProtKB-KW
- phospholipid metabolic process Source: InterPro
Keywordsi
Molecular function | Blood coagulation cascade inhibiting toxin, Hemostasis impairing toxin, Hydrolase, Platelet aggregation inhibiting toxin, Toxin |
Biological process | Angiogenesis, Lipid degradation, Lipid metabolism |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Acidic phospholipase A2 CC-PLA2-2 (EC:3.1.1.4)Short name: svPLA2 Alternative name(s): Phosphatidylcholine 2-acylhydrolase |
Organismi | Cerastes cerastes (Horned desert viper) |
Taxonomic identifieri | 8697 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Viperinae › Cerastes |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | 1 PublicationAdd BLAST | 16 | |
ChainiPRO_0000376917 | 17 – 136 | Acidic phospholipase A2 CC-PLA2-2Add BLAST | 120 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 129 | By similarity | ||
Disulfide bondi | 44 ↔ 60 | By similarity | ||
Disulfide bondi | 59 ↔ 109 | By similarity | ||
Disulfide bondi | 65 ↔ 136 | By similarity | ||
Disulfide bondi | 66 ↔ 102 | By similarity | ||
Disulfide bondi | 73 ↔ 95 | By similarity | ||
Disulfide bondi | 90 ↔ 100 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinExpressioni
Tissue specificityi
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00118, PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MRTLWIVAVW LMGVEGNLFQ FGKMIKHKTG KSALLSYSGN PCYCGWGGQG
60 70 80 90 100
PPQDATDHCC FVHDCCYGEE NACYPKTAFT LKFENQIIIC DEDPCNYAVC
110 120 130
MCDRVAAICG GENVATSDAK YLFYRSMGCE EESVQC
Mass spectrometryi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ754187 mRNA Translation: ACO92623.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ754187 mRNA Translation: ACO92623.1 |
3D structure databases
SMRi | P0CAS0 |
ModBasei | Search... |
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00118, PA2_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PA2A2_CERCE | |
Accessioni | P0CAS0Primary (citable) accession number: P0CAS0 Secondary accession number(s): C3UWD0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 16, 2009 |
Last sequence update: | October 3, 2012 | |
Last modified: | February 23, 2022 | |
This is version 36 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families