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Protein

DNA-directed RNA polymerase II subunit GRINL1A

Gene

POLR2M

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Isoform 1 appears to be a stable component of the Pol II(G) complex form of RNA polymerase II (Pol II). Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in the Mediator complex-dependent regulation of transcription activation. Isoform 1 acts in vitro as a negative regulator of transcriptional activation; this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II.1 Publication

Miscellaneous

The adjacent MYZAP and POLR2M genes are part of a complex transcription unit. The respective transcripts derive from different promoters and are alternatively spliced. In human, some transcripts of the upstream promoter of MYZAP use exons of the downstream POLR2M gene.

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • maintenance of ER location Source: GO_Central

Keywordsi

Biological processTranscription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit GRINL1A
Alternative name(s):
DNA-directed RNA polymerase II subunit M
Glutamate receptor-like protein 1A
Gene namesi
Name:POLR2M
Synonyms:GRINL1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000255529.7
HGNCiHGNC:14862 POLR2M
MIMi606485 gene
neXtProtiNX_P0CAP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi100820829
145781
OpenTargetsiENSG00000255529
PharmGKBiPA28986

Polymorphism and mutation databases

BioMutaiPOLR2M
DMDMi238064986

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_50000895461 – 368DNA-directed RNA polymerase II subunit GRINL1AAdd BLAST368

Proteomic databases

EPDiP0CAP2
PaxDbiP0CAP2
PeptideAtlasiP0CAP2
PRIDEiP0CAP2
ProteomicsDBi52428
52429 [P0CAP2-2]
52430 [P0CAP2-3]

PTM databases

iPTMnetiP0CAP2
PhosphoSitePlusiP0CAP2

Expressioni

Tissue specificityi

Detected in adult an fetal brain. Detected in heart, kidney, skeletal muscle, small intestine, lung, prostate and testis.1 Publication

Gene expression databases

BgeeiENSG00000255529 Expressed in 234 organ(s), highest expression level in kidney
ExpressionAtlasiP0CAP2 baseline and differential
GenevisibleiP0CAP2 HS

Organism-specific databases

HPAiHPA068141

Interactioni

Subunit structurei

Isoform 1 is a component of the Pol II(G) complex, which contains the RNA polymerase II (Pol II) core complex subunits and POLR2M isoform 1. Pol II(G) appears to be an abundant form of Pol II.

Protein-protein interaction databases

BioGridi123498, 66 interactors
DIPiDIP-61933N
IntActiP0CAP2, 10 interactors
MINTiP0CAP2
STRINGi9606.ENSP00000299638

Structurei

3D structure databases

ProteinModelPortaliP0CAP2
SMRiP0CAP2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili301 – 335Sequence analysisAdd BLAST35

Sequence similaritiesi

Belongs to the GRINL1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IVF6 Eukaryota
ENOG410ZKMZ LUCA
GeneTreeiENSGT00530000063232
HOVERGENiHBG105025
InParanoidiP0CAP2
KOiK21987
OMAiHYMTVLE
OrthoDBiEOG091G0HQJ
PhylomeDBiP0CAP2
TreeFamiTF332945

Family and domain databases

InterProiView protein in InterPro
IPR026213 GRINL1
PfamiView protein in Pfam
PF15328 GCOM2, 1 hit
PRINTSiPR02085 POLR2GRINL1

Sequences (6+)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 6 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P0CAP2-1) [UniParc]FASTAAdd to basket
Also known as: Gdown1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCSLPRGFEP QAPEDLAQRS LVELREMLKR QERLLRNEKF ICKLPDKGKK
60 70 80 90 100
IFDSFAKLKA AIAECEEVRR KSELFNPVSL DCKLRQKAIA EVDVGTDKAQ
110 120 130 140 150
NSDPILDTSS LVPGCSSVDN IKSSQTSQNQ GLGRPTLEGD EETSEVEYTV
160 170 180 190 200
NKGPASSNRD RVPPSSEASE HHPRHRVSSQ AEDTSSSFDN LFIDRLQRIT
210 220 230 240 250
IADQGEQQSE ENASTKNLTG LSSGTEKKPH YMEVLEMRAK NPVPQLRKFK
260 270 280 290 300
TNVLPFRQND SSSHCQKSGS PISSEERRRR DKQHLDDITA ARLLPLHHMP
310 320 330 340 350
TQLLSIEESL ALQKQQKQNY EEMQAKLAAQ KLAERLNIKM RSYNPEGESS
360
GRYREVRDED DDWSSDEF
Length:368
Mass (Da):41,740
Last modified:May 26, 2009 - v1
Checksum:iB2F3ADAC21793D1E
GO
Isoform 2 (identifier: P0CAP2-2) [UniParc]FASTAAdd to basket
Also known as: Gdown6

The sequence of this isoform differs from the canonical sequence as follows:
     39-195: Missing.

Show »
Length:211
Mass (Da):24,533
Checksum:i02CD8ED77ADC9983
GO
Isoform 3 (identifier: P0CAP2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     323-368: MQAKLAAQKL...EDDDWSSDEF → RPFYSPQYRS...MGSLALLTKL

Show »
Length:363
Mass (Da):40,734
Checksum:i9EBE33AF873AC052
GO
Isoform 4 (identifier: Q6EEV4-1) [UniParc]FASTAAdd to basket
Also known as: Gdown4
The sequence of this isoform can be found in the external entry Q6EEV4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:148
Mass (Da):15,131
GO
Isoform 5 (identifier: Q6EEV4-2) [UniParc]FASTAAdd to basket
Also known as: Gdown3
The sequence of this isoform can be found in the external entry Q6EEV4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:86
Mass (Da):8,827
GO
Isoform 11 (identifier: P0CAP1-11) [UniParc]FASTAAdd to basket
Also known as: Gcom1, GRINL1A complex locus protein 1
The sequence of this isoform can be found in the external entry P0CAP1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Based on a naturally occurring readthrough transcript which produces a MYZAP-POLR2M fusion protein.
Length:550
Mass (Da):64,046
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PP13E9PP13_HUMAN
DNA-directed RNA polymerase II subu...
POLR2M
148Annotation score:
E9PSG9E9PSG9_HUMAN
DNA-directed RNA polymerase II subu...
POLR2M
159Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171H → D in CAB43263 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03738739 – 195Missing in isoform 2. 2 PublicationsAdd BLAST157
Alternative sequenceiVSP_037388323 – 368MQAKL…SSDEF → RPFYSPQYRSSMNLLSLAAA AKDTRGSKSGKMGSLALLTK L in isoform 3. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326773 mRNA Translation: AAK92284.2
AY207007 mRNA Translation: AAO39707.1
AY353061 mRNA Translation: AAQ76837.1
AK074767 mRNA Translation: BAC11193.1
AK074955 mRNA Translation: BAC11313.1
AK128618 mRNA Translation: BAC87533.1
AC090651 Genomic DNA No translation available.
CH471082 Genomic DNA Translation: EAW77525.1
BC001510 mRNA Translation: AAH01510.1
AL050091 mRNA Translation: CAB43263.1
CCDSiCCDS32252.1 [P0CAP2-1]
CCDS42045.1 [P0CAP2-2]
PIRiT08740
RefSeqiNP_001018112.1, NM_001018102.2 [P0CAP2-2]
NP_056347.1, NM_015532.4 [P0CAP2-1]
UniGeneiHs.437256
Hs.702937
Hs.711270

Genome annotation databases

EnsembliENST00000299638; ENSP00000299638; ENSG00000255529 [P0CAP2-1]
ENST00000380557; ENSP00000369930; ENSG00000255529 [P0CAP2-2]
ENST00000482852; ENSP00000432615; ENSG00000255529 [P0CAP2-3]
GeneIDi81488
KEGGihsa:81488
UCSCiuc002aet.6 human [P0CAP2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326773 mRNA Translation: AAK92284.2
AY207007 mRNA Translation: AAO39707.1
AY353061 mRNA Translation: AAQ76837.1
AK074767 mRNA Translation: BAC11193.1
AK074955 mRNA Translation: BAC11313.1
AK128618 mRNA Translation: BAC87533.1
AC090651 Genomic DNA No translation available.
CH471082 Genomic DNA Translation: EAW77525.1
BC001510 mRNA Translation: AAH01510.1
AL050091 mRNA Translation: CAB43263.1
CCDSiCCDS32252.1 [P0CAP2-1]
CCDS42045.1 [P0CAP2-2]
PIRiT08740
RefSeqiNP_001018112.1, NM_001018102.2 [P0CAP2-2]
NP_056347.1, NM_015532.4 [P0CAP2-1]
UniGeneiHs.437256
Hs.702937
Hs.711270

3D structure databases

ProteinModelPortaliP0CAP2
SMRiP0CAP2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123498, 66 interactors
DIPiDIP-61933N
IntActiP0CAP2, 10 interactors
MINTiP0CAP2
STRINGi9606.ENSP00000299638

PTM databases

iPTMnetiP0CAP2
PhosphoSitePlusiP0CAP2

Polymorphism and mutation databases

BioMutaiPOLR2M
DMDMi238064986

Proteomic databases

EPDiP0CAP2
PaxDbiP0CAP2
PeptideAtlasiP0CAP2
PRIDEiP0CAP2
ProteomicsDBi52428
52429 [P0CAP2-2]
52430 [P0CAP2-3]

Protocols and materials databases

DNASUi81488
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299638; ENSP00000299638; ENSG00000255529 [P0CAP2-1]
ENST00000380557; ENSP00000369930; ENSG00000255529 [P0CAP2-2]
ENST00000482852; ENSP00000432615; ENSG00000255529 [P0CAP2-3]
GeneIDi81488
KEGGihsa:81488
UCSCiuc002aet.6 human [P0CAP2-1]

Organism-specific databases

CTDi81488
DisGeNETi100820829
145781
EuPathDBiHostDB:ENSG00000255529.7
GeneCardsiPOLR2M
HGNCiHGNC:14862 POLR2M
HPAiHPA068141
MIMi606485 gene
neXtProtiNX_P0CAP2
OpenTargetsiENSG00000255529
PharmGKBiPA28986
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IVF6 Eukaryota
ENOG410ZKMZ LUCA
GeneTreeiENSGT00530000063232
HOVERGENiHBG105025
InParanoidiP0CAP2
KOiK21987
OMAiHYMTVLE
OrthoDBiEOG091G0HQJ
PhylomeDBiP0CAP2
TreeFamiTF332945

Miscellaneous databases

GenomeRNAii81488
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000255529 Expressed in 234 organ(s), highest expression level in kidney
ExpressionAtlasiP0CAP2 baseline and differential
GenevisibleiP0CAP2 HS

Family and domain databases

InterProiView protein in InterPro
IPR026213 GRINL1
PfamiView protein in Pfam
PF15328 GCOM2, 1 hit
PRINTSiPR02085 POLR2GRINL1
ProtoNetiSearch...

Entry informationi

Entry nameiGRL1A_HUMAN
AccessioniPrimary (citable) accession number: P0CAP2
Secondary accession number(s): Q6EER8
, Q6EES2, Q6EEV3, Q6EF00, Q6EF01, Q6EF02, Q6EF46, Q6EFN8, Q6EM48, Q6K046, Q6K050, Q6K051, Q6ZQZ3, Q8NC58, Q8NCF3, Q96DI5, Q96JB7, Q96NF5, Q9Y3V6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: November 7, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
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