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UniProtKB - P0C956 (GEL4_ASPFU)
Protein
1,3-beta-glucanosyltransferase gel4
Gene
gel4
Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Functioni
Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall morphogenesis (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 106 | 1,3-beta-glucan 1; donor substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 174 | 1,3-beta-glucan 1; donor substrateBy similarity | 1 | |
Active sitei | 175 | Proton donorBy similarity | 1 | |
Binding sitei | 175 | 1,3-beta-glucan 2; acceptor substrateBy similarity | 1 | |
Binding sitei | 217 | 1,3-beta-glucan 2; acceptor substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 222 | 1,3-beta-glucan 2; acceptor substrateBy similarity | 1 | |
Active sitei | 277 | NucleophileBy similarity | 1 | |
Binding sitei | 309 | 1,3-beta-glucan 1; donor substrateBy similarity | 1 |
GO - Molecular functioni
- 1,3-beta-glucanosyltransferase activity Source: GO_Central
GO - Biological processi
- cell wall (1->3)-beta-D-glucan biosynthetic process Source: GO_Central
- fungal-type cell wall (1->3)-beta-D-glucan biosynthetic process Source: GO_Central
- fungal-type cell wall organization Source: GO_Central
Keywordsi
Molecular function | Transferase |
Protein family/group databases
CAZyi | CBM43, Carbohydrate-Binding Module Family 43 GH72, Glycoside Hydrolase Family 72 |
Names & Taxonomyi
Protein namesi | Recommended name: 1,3-beta-glucanosyltransferase gel4 (EC:2.4.1.-)Alternative name(s): Glucan elongating glucanosyltransferase 4 |
Gene namesi | Name:gel4 ORF Names:AFUA_2G05340 |
Organismi | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
Taxonomic identifieri | 330879 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; GPI-anchor By similarity
Cell Wall
- fungal-type cell wall Source: GO_Central
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- anchored component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000245553 | 26 – 519 | 1,3-beta-glucanosyltransferase gel4Add BLAST | 494 | |
PropeptideiPRO_0000245554 | 520 – 548 | Removed in mature formSequence analysisAdd BLAST | 29 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 51 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 69 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 88 ↔ 117 | By similarity | ||
Glycosylationi | 181 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 231 ↔ 364 | By similarity | ||
Disulfide bondi | 249 ↔ 280 | By similarity | ||
Disulfide bondi | 386 ↔ 437 | By similarity | ||
Disulfide bondi | 395 ↔ 461 | By similarity | ||
Disulfide bondi | 414 ↔ 419 | By similarity | ||
Glycosylationi | 425 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 519 | GPI-like-anchor amidated alanineSequence analysis | 1 |
Post-translational modificationi
The GPI-like anchor contains a phosphoceramide lipid group.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
PRIDEi | P0C956 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 133 – 141 | 1,3-beta-glucan 1 binding; donor substrateBy similarity | 9 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 72 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QPST, Eukaryota |
HOGENOMi | CLU_021855_2_1_1 |
InParanoidi | P0C956 |
OMAi | CATQMSA |
OrthoDBi | 728071at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004886, Glucanosyltransferase IPR017853, Glycoside_hydrolase_SF IPR012946, X8 |
PANTHERi | PTHR31468, PTHR31468, 1 hit |
Pfami | View protein in Pfam PF03198, Glyco_hydro_72, 1 hit PF07983, X8, 1 hit |
SMARTi | View protein in SMART SM00768, X8, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0C956-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFVYAAAGA SLVGSALATL PVIEAKVLKA CCWTIALDAN SYGNKFFYSN
60 70 80 90 100
NGTEFFIRGV AYQQEYQANG TSTENSDYTD PLANVDNCKR DIPYLKQLRT
110 120 130 140 150
NVIRTYAVDP TKDHDECMKL LDDAGIYLIT DLSAPSESIN RADPAWNTDL
160 170 180 190 200
YKRYTSVIDA FAKYSNVIGF FAGNEVANDN NNTNSIAYVK AAVRDMKSYI
210 220 230 240 250
KSKDYRSSLL VGYATDDDAH IRADLADYLV CGDKESSIDM FGYNIYEWCG
260 270 280 290 300
DSSFEKSGYK DRTEEFSKYP VPAFFSEYGC IDPKPRKFTD VAALYGPQMN
310 320 330 340 350
DVWSGGIVYM YFQEANDYGL VSVSGDNVKT KEDFSYLSVQ MQKVTATGVN
360 370 380 390 400
SASYTASNTA VPTCPSVGAK WEASNKLPPS PNSELCDCMV ETLSCTVKDS
410 420 430 440 450
VDEKEYGDLF DYLCAAGVCG GINSNSTSGD YGAYSVCSAK QKLSFVMNQY
460 470 480 490 500
YKKNNKAATA CDFDGKAQTK KGADASGSCA SLISQAGTAG TGSVTAGATG
510 520 530 540
SSGSGSASET SKGAAGVAAS PMAVKVGNWQ FGAYIATALF AGVGMLVL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAHF01000008 Genomic DNA Translation: EAL87626.1 |
RefSeqi | XP_749664.1, XM_744571.1 |
Genome annotation databases
EnsemblFungii | EAL87626; EAL87626; AFUA_2G05340 |
GeneIDi | 3506753 |
KEGGi | afm:AFUA_2G05340 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAHF01000008 Genomic DNA Translation: EAL87626.1 |
RefSeqi | XP_749664.1, XM_744571.1 |
3D structure databases
SMRi | P0C956 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 746128.CADAFUBP00002184 |
Protein family/group databases
CAZyi | CBM43, Carbohydrate-Binding Module Family 43 GH72, Glycoside Hydrolase Family 72 |
Proteomic databases
PRIDEi | P0C956 |
Genome annotation databases
EnsemblFungii | EAL87626; EAL87626; AFUA_2G05340 |
GeneIDi | 3506753 |
KEGGi | afm:AFUA_2G05340 |
Phylogenomic databases
eggNOGi | ENOG502QPST, Eukaryota |
HOGENOMi | CLU_021855_2_1_1 |
InParanoidi | P0C956 |
OMAi | CATQMSA |
OrthoDBi | 728071at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004886, Glucanosyltransferase IPR017853, Glycoside_hydrolase_SF IPR012946, X8 |
PANTHERi | PTHR31468, PTHR31468, 1 hit |
Pfami | View protein in Pfam PF03198, Glyco_hydro_72, 1 hit PF07983, X8, 1 hit |
SMARTi | View protein in SMART SM00768, X8, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GEL4_ASPFU | |
Accessioni | P0C956Primary (citable) accession number: P0C956 Secondary accession number(s): Q4WHH9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | May 5, 2009 | |
Last modified: | February 23, 2022 | |
This is version 66 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families