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Protein

Basic phospholipase A2 BmTX-I

Gene
N/A
Organism
Bothrops moojeni (Lance-headed viper) (Caissaca)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that shows enzymatic activity in the presence of a synthetic substrate. In vitro, blocks the neuromuscular transmission in young chick biventer cervicis preparations. In mice, induces myonecrosis and a systemic interleukin-6 response upon intramuscular injection. Also induces edema and exerts a strong proinflammatory effect. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+1 PublicationNote: Binds 1 Ca2+ ion.1 Publication

Activity regulationi

Inhibited by magnesium, cadmium and manganese ions. Also inhibited by crotapotin.1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Temperature dependencei

Optimum temperature is 35-45 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi27Calcium; via carbonyl oxygenBy similarity1
Metal bindingi29Calcium; via carbonyl oxygenBy similarity1
Metal bindingi31Calcium; via carbonyl oxygenBy similarity1
Active sitei48By similarity1
Metal bindingi49CalciumBy similarity1
Active sitei89By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Myotoxin, Neurotoxin, Toxin
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Basic phospholipase A2 BmTX-I (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
OrganismiBothrops moojeni (Lance-headed viper) (Caissaca)
Taxonomic identifieri98334 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003594361 – 121Basic phospholipase A2 BmTX-IAdd BLAST121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 114By similarity
Disulfide bondi28 ↔ 45By similarity
Disulfide bondi44 ↔ 95By similarity
Disulfide bondi50 ↔ 121By similarity
Disulfide bondi51 ↔ 88By similarity
Disulfide bondi58 ↔ 82By similarity
Disulfide bondi76 ↔ 86By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

SMRiP0C8M1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG008137

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

P0C8M1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
DLWQFNKMIK KEVGKLPFPF YGAYGCYCGW GGRGEKPKDG TDRCCFVHDC
60 70 80 90 100
CYKKLTGCPK WDDRYSYSWK DITIVCGEDL PCEEICECDR AAAVCFYENL
110 120
GTYNKKYMKH LKPCKKADYP C
Length:121
Mass (Da):14,081
Last modified:January 20, 2009 - v1
Checksum:i4441455A8D14F766
GO

Mass spectrometryi

Molecular mass is 14238.71 Da from positions 1 - 121. Determined by MALDI. 1 Publication

Similar proteinsi

Cross-referencesi

3D structure databases

SMRiP0C8M1
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPA2B1_BOTMO
AccessioniPrimary (citable) accession number: P0C8M1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 20, 2009
Last modified: September 12, 2018
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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