UniProtKB - P0C871 (PA24B_MOUSE)
Protein
Cytosolic phospholipase A2 beta
Gene
Pla2g4b
Organism
Mus musculus (Mouse)
Status
Functioni
Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A (By similarity).By similarity
Caution
Most tissues also express read-through transcripts from this gene into the upstream gene (Jmjd7), some of which may encode fusion proteins.Curated
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+EC:3.1.1.4
Activity regulationi
Stimulated by cytosolic Ca2+.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 333 | NucleophileBy similarity | 1 | |
Active sitei | 613 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- calcium-dependent phospholipase A2 activity Source: MGI
- calcium-dependent phospholipid binding Source: GO_Central
- calcium ion binding Source: GO_Central
- lysophospholipase activity Source: MGI
- phospholipase A2 activity Source: MGI
- phospholipase A2 activity (consuming 1,2-dipalmitoylphosphatidylcholine) Source: UniProtKB-EC
- phospholipase A2 activity consuming 1,2-dioleoylphosphatidylethanolamine) Source: UniProtKB-EC
GO - Biological processi
- glycerophospholipid catabolic process Source: MGI
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic phospholipase A2 beta (EC:3.1.1.4)Short name: cPLA2-beta Alternative name(s): Phospholipase A2 group IVB |
Gene namesi | Name:Pla2g4b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2384819 Pla2g4b |
Subcellular locationi
Cytosol
Other locations
- Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity
Cytosol
- cytosol Source: MGI
Other locations
- cytoplasm Source: GO_Central
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247022 | 1 – 782 | Cytosolic phospholipase A2 betaAdd BLAST | 782 |
Proteomic databases
MaxQBi | P0C871 |
PaxDbi | P0C871 |
PRIDEi | P0C871 |
PTM databases
iPTMneti | P0C871 |
PhosphoSitePlusi | P0C871 |
Interactioni
Protein-protein interaction databases
BioGridi | 229231, 1 interactor |
STRINGi | 10090.ENSMUSP00000118458 |
Structurei
3D structure databases
ProteinModelPortali | P0C871 |
SMRi | P0C871 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 102 | C2PROSITE-ProRule annotationAdd BLAST | 102 | |
Domaini | 245 – 782 | PLA2cPROSITE-ProRule annotationAdd BLAST | 538 |
Domaini
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity
Phylogenomic databases
eggNOGi | KOG1028 Eukaryota KOG1325 Eukaryota ENOG410XR72 LUCA |
HOGENOMi | HOG000231788 |
HOVERGENi | HBG080412 |
InParanoidi | P0C871 |
KOi | K16342 |
OrthoDBi | 302848at2759 |
Family and domain databases
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016035 Acyl_Trfase/lysoPLipase IPR000008 C2_dom IPR035892 C2_domain_sf IPR040723 cPLA2_C2 IPR002642 LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF00168 C2, 1 hit PF18695 cPLA2_C2, 1 hit PF01735 PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00239 C2, 1 hit SM00022 PLAc, 1 hit |
SUPFAMi | SSF52151 SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50004 C2, 1 hit PS51210 PLA2C, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P0C871-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQAKVPETCL LTVRVLRASG LPSKDLVTSS DCYVTLNLPT ASSHTLQTRT
60 70 80 90 100
VKNSRNPVWN QNFHFRIHRQ LKNVMELKVF DHDLVTRDDP VLSVLFDVGT
110 120 130 140 150
LQIGTQRQSF SLGTQEKGCL EVEFRLQTLT DCEEQLISNG IVVARELSCL
160 170 180 190 200
HVELKRTGDP KRSERKVQLV VAGACEGPQD ASAGTGSFHF HYPACWEQEL
210 220 230 240 250
NVHLQDDPHE QLKVPLRTLP SSQLVRLVFP TSQEPLMRLE LKKEEGPKEL
260 270 280 290 300
AVRLGCGPCP EEQAFLSKRK QVVAAALKKA LQLDQDLHED EIPVIAVMAT
310 320 330 340 350
GGGIRAMTSL YGQLAGLQEL GLLDCISYIT GASGSTWALA NLYEDPEWSQ
360 370 380 390 400
KDLAGPTEVL KTQVTKSKLG ALAPSQLWRY RQELAERARL GHPTCFTNLW
410 420 430 440 450
ALINEALLHD KPHEHKLSDQ REALSRGQNP LPIYCALNSK EQGLSTFDFG
460 470 480 490 500
EWCEFSPYEV GFPKYGAFIS SELFGSEFFM GRLVKQLPES RICFLEGIWS
510 520 530 540 550
NLFAASLQDS LYWSSEPSQF WDRWAQDQAN LDKEQVPHLK IAEPPTMAGR
560 570 580 590 600
IAELFTDLLT KRPLAHATHN FTRGLHFHKD YFQNSHFSAW KASKLDRLPN
610 620 630 640 650
QLTPTEPHLC LLDVGYLINT SCPPLLQPTR DVDLILSLDY NLYGAFQQLQ
660 670 680 690 700
LLSRFCQEQG IPFPSISPSP EEQRQPQECH LFCDPAQPEA PAVLHFPLVN
710 720 730 740 750
DSFQDYSAPG VPRTSEEKAA GEVNLSSSDS PYHYTKVTYS QEDVDKLLRL
760 770 780
THYNICNNQD RLREAMHQAV QRRRKRKQFR PE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB7ZCM8 | B7ZCM8_MOUSE | Phospholipase A2 | Pla2g4b | 791 | Annotation score: |
Sequence cautioni
The sequence CAM22684 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 75 | M → I in BC098210 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 79 | V → A in BC098210 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 605 | T → A in AAH42758 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 617 | L → F in AAH42758 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL833774 Genomic DNA Translation: CAM22684.1 Sequence problems. BC042758 mRNA Translation: AAH42758.1 BC098210 mRNA No translation available. |
RefSeqi | NP_663353.3, NM_145378.3 |
UniGenei | Mm.41467 |
Genome annotation databases
GeneIDi | 211429 |
KEGGi | mmu:211429 |
UCSCi | uc008luz.2 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL833774 Genomic DNA Translation: CAM22684.1 Sequence problems. BC042758 mRNA Translation: AAH42758.1 BC098210 mRNA No translation available. |
RefSeqi | NP_663353.3, NM_145378.3 |
UniGenei | Mm.41467 |
3D structure databases
ProteinModelPortali | P0C871 |
SMRi | P0C871 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 229231, 1 interactor |
STRINGi | 10090.ENSMUSP00000118458 |
PTM databases
iPTMneti | P0C871 |
PhosphoSitePlusi | P0C871 |
Proteomic databases
MaxQBi | P0C871 |
PaxDbi | P0C871 |
PRIDEi | P0C871 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
GeneIDi | 211429 |
KEGGi | mmu:211429 |
UCSCi | uc008luz.2 mouse |
Organism-specific databases
CTDi | 100137049 |
MGIi | MGI:2384819 Pla2g4b |
Phylogenomic databases
eggNOGi | KOG1028 Eukaryota KOG1325 Eukaryota ENOG410XR72 LUCA |
HOGENOMi | HOG000231788 |
HOVERGENi | HBG080412 |
InParanoidi | P0C871 |
KOi | K16342 |
OrthoDBi | 302848at2759 |
Miscellaneous databases
PROi | PR:P0C871 |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016035 Acyl_Trfase/lysoPLipase IPR000008 C2_dom IPR035892 C2_domain_sf IPR040723 cPLA2_C2 IPR002642 LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF00168 C2, 1 hit PF18695 cPLA2_C2, 1 hit PF01735 PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00239 C2, 1 hit SM00022 PLAc, 1 hit |
SUPFAMi | SSF52151 SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50004 C2, 1 hit PS51210 PLA2C, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | PA24B_MOUSE | |
Accessioni | P0C871Primary (citable) accession number: P0C871 Secondary accession number(s): A2AP62 Q91W88 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | September 2, 2008 | |
Last modified: | February 13, 2019 | |
This is version 82 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot