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UniProtKB - P0C7L2 (PAAJ_ECOLI)
Protein
3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase
Gene
paaJ
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the thiolytic cleavage of the beta-keto C8 intermediate 3-oxo-5,6-dehydrosuberyl-CoA with CoA to yield the C6 intermediate 2,3-dehydroadipyl-CoA and acetyl-CoA. Besides it catalyzes also the last step of the pathway, in which 3-oxoadipyl-CoA similarly is cleaved to acetyl-CoA and succinyl-CoA.
3 PublicationsCatalytic activityi
: phenylacetate degradation Pathwayi
This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 90 | Acyl-thioester intermediateBy similarity | 1 | |
Active sitei | 357 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 387 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 3-oxoadipyl-CoA thiolase activity Source: UniProtKB
- acetyl-CoA C-acyltransferase activity Source: GO_Central
- transferase activity Source: EcoCyc
GO - Biological processi
- 3,4-dihydroxybenzoate catabolic process Source: InterPro
- cellular response to DNA damage stimulus Source: EcoliWiki
- fatty acid beta-oxidation Source: GO_Central
- phenylacetate catabolic process Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:G6718-MONOMER |
UniPathwayi | UPA00930 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:paaJ Synonyms:paaE, ydbW Ordered Locus Names:b1397, JW1392 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutants are unable to use phenylacetate as a carbon source.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206421 | 1 – 401 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolaseAdd BLAST | 401 |
Proteomic databases
PaxDbi | P0C7L2 |
PRIDEi | P0C7L2 |
Expressioni
Inductioni
Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263012, 239 interactors |
IntActi | P0C7L2, 5 interactors |
STRINGi | 511145.b1397 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0183, Bacteria |
HOGENOMi | CLU_031026_2_2_6 |
InParanoidi | P0C7L2 |
PhylomeDBi | P0C7L2 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR012793, PcaF IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit TIGR02430, pcaF, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P0C7L2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MREAFICDGI RTPIGRYGGA LSSVRADDLA AIPLRELLVR NPRLDAECID
60 70 80 90 100
DVILGCANQA GEDNRNVARM ATLLAGLPQS VSGTTINRLC GSGLDALGFA
110 120 130 140 150
ARAIKAGDGD LLIAGGVESM SRAPFVMGKA ASAFSRQAEM FDTTIGWRFV
160 170 180 190 200
NPLMAQQFGT DSMPETAENV AELLKISRED QDSFALRSQQ RTAKAQSSGI
210 220 230 240 250
LAEEIVPVVL KNKKGVVTEI QHDEHLRPET TLEQLRGLKA PFRANGVITA
260 270 280 290 300
GNASGVNDGA AALIIASEQM AAAQGLTPRA RIVAMATAGV EPRLMGLGPV
310 320 330 340 350
PATRRVLERA GLSIHDMDVI ELNEAFAAQA LGVLRELGLP DDAPHVNPNG
360 370 380 390 400
GAIALGHPLG MSGARLALAA SHELHRRNGR YALCTMCIGV GQGIAMILER
V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74479.1 AP009048 Genomic DNA Translation: BAA15002.1 |
PIRi | H64890 |
RefSeqi | NP_415915.1, NC_000913.3 WP_001206197.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74479; AAC74479; b1397 BAA15002; BAA15002; BAA15002 |
GeneIDi | 946121 |
KEGGi | ecj:JW1392 eco:b1397 |
PATRICi | fig|1411691.4.peg.874 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74479.1 AP009048 Genomic DNA Translation: BAA15002.1 |
PIRi | H64890 |
RefSeqi | NP_415915.1, NC_000913.3 WP_001206197.1, NZ_SSZK01000012.1 |
3D structure databases
SMRi | P0C7L2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263012, 239 interactors |
IntActi | P0C7L2, 5 interactors |
STRINGi | 511145.b1397 |
Proteomic databases
PaxDbi | P0C7L2 |
PRIDEi | P0C7L2 |
Genome annotation databases
EnsemblBacteriai | AAC74479; AAC74479; b1397 BAA15002; BAA15002; BAA15002 |
GeneIDi | 946121 |
KEGGi | ecj:JW1392 eco:b1397 |
PATRICi | fig|1411691.4.peg.874 |
Organism-specific databases
EchoBASEi | EB3507 |
Phylogenomic databases
eggNOGi | COG0183, Bacteria |
HOGENOMi | CLU_031026_2_2_6 |
InParanoidi | P0C7L2 |
PhylomeDBi | P0C7L2 |
Enzyme and pathway databases
UniPathwayi | UPA00930 |
BioCyci | EcoCyc:G6718-MONOMER |
Miscellaneous databases
PROi | PR:P0C7L2 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR012793, PcaF IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit TIGR02430, pcaF, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PAAJ_ECOLI | |
Accessioni | P0C7L2Primary (citable) accession number: P0C7L2 Secondary accession number(s): O53017, P77525 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | February 23, 2022 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families