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Protein

Ghrelin O-acyltransferase

Gene

Mboat4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei307Curated1
Active sitei338Curated1

GO - Molecular functioni

  • serine O-acyltransferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin

Names & Taxonomyi

Protein namesi
Recommended name:
Ghrelin O-acyltransferase (EC:2.3.1.-)
Alternative name(s):
Membrane-bound O-acyltransferase domain-containing protein 4
Short name:
O-acyltransferase domain-containing protein 4
Gene namesi
Name:Mboat4
Synonyms:Gm171, Goat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2685017 Mboat4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
Transmembranei45 – 65HelicalSequence analysisAdd BLAST21
Transmembranei163 – 183HelicalSequence analysisAdd BLAST21
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Transmembranei331 – 351HelicalSequence analysisAdd BLAST21
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Transmembranei408 – 428HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display a complete lack of octanoylated ghrelin in blood.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi181C → A: No effect on ability to acylate ghrelin. 1 Publication1
Mutagenesisi307N → A: Abolished ability to acylate ghrelin. 1 Publication1
Mutagenesisi338H → A: Abolished ability to acylate ghrelin. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1255134

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003290731 – 435Ghrelin O-acyltransferaseAdd BLAST435

Proteomic databases

PaxDbiP0C7A3
PRIDEiP0C7A3

PTM databases

iPTMnetiP0C7A3
PhosphoSitePlusiP0C7A3

Expressioni

Tissue specificityi

Highly expressed in stomach. Lower expression in small intestine and colon. Very low expression in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000071113 Expressed in 108 organ(s), highest expression level in bone marrow
GenevisibleiP0C7A3 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092988

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2704 Eukaryota
COG5202 LUCA
GeneTreeiENSGT00550000074565
HOGENOMiHOG000113492
HOVERGENiHBG108120
InParanoidiP0C7A3
OMAiKWNQSTA
OrthoDBiEOG091G07L0
PhylomeDBiP0C7A3
TreeFamiTF314906

Family and domain databases

InterProiView protein in InterPro
IPR004299 MBOAT_fam
PfamiView protein in Pfam
PF03062 MBOAT, 1 hit

Sequencei

Sequence statusi: Complete.

P0C7A3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDWLQLFFLH PLSFYQGAAF PFALLFNYLC ILDTFSTRAR YLFLLAGGGV
60 70 80 90 100
LAFAAMGPYS LLIFIPALCA VALVSFLSPQ EVHRLTFFFQ MGWQTLCHLG
110 120 130 140 150
LHYTEYYLGE PPPVRFYITL SSLMLLTQRV TSLSLDICEG KVEAPRRGIR
160 170 180 190 200
SKSSFSEHLW DALPHFSYLL FFPALLGGSL CSFRRFQACV QRSSSLYPSI
210 220 230 240 250
SFRALTWRGL QILGLECLKV ALRSAVSAGA GLDDCQRLEC IYLMWSTAWL
260 270 280 290 300
FKLTYYSHWI LDDSLLHAAG FGAEAGQGPG EEGYVPDVDI WTLETTHRIS
310 320 330 340 350
LFARQWNRST ALWLRRLVFR KSRRWPLLQT FAFSAWWHGL HPGQVFGFLC
360 370 380 390 400
WSVMVKADYL IHTFANVCIR SWPLRLLYRA LTWAHTQLII AYIMLAVEGR
410 420 430
SLSSLCQLCC SYNSLFPVMY GLLLFLLAER KDKRN
Length:435
Mass (Da):49,879
Last modified:April 8, 2008 - v1
Checksum:iC2B7E69E35185620
GO

Sequence cautioni

The sequence AAI47845 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI47850 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31I → V in AAI47845 (PubMed:15489334).Curated1
Sequence conflicti31I → V in AAI47850 (PubMed:15489334).Curated1
Sequence conflicti53F → L in AAI47845 (PubMed:15489334).Curated1
Sequence conflicti53F → L in AAI47850 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU721729 mRNA Translation: ACD93144.1
EU518496 mRNA Translation: ACB05874.1
AC167537 Genomic DNA No translation available.
BC147844 mRNA Translation: AAI47845.1 Different initiation.
BC147849 mRNA Translation: AAI47850.1 Different initiation.
CCDSiCCDS52540.1
RefSeqiNP_001119786.1, NM_001126314.2
UniGeneiMm.324008

Genome annotation databases

EnsembliENSMUST00000095345; ENSMUSP00000092988; ENSMUSG00000071113
GeneIDi234155
KEGGimmu:234155
UCSCiuc012gch.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU721729 mRNA Translation: ACD93144.1
EU518496 mRNA Translation: ACB05874.1
AC167537 Genomic DNA No translation available.
BC147844 mRNA Translation: AAI47845.1 Different initiation.
BC147849 mRNA Translation: AAI47850.1 Different initiation.
CCDSiCCDS52540.1
RefSeqiNP_001119786.1, NM_001126314.2
UniGeneiMm.324008

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092988

Chemistry databases

ChEMBLiCHEMBL1255134

PTM databases

iPTMnetiP0C7A3
PhosphoSitePlusiP0C7A3

Proteomic databases

PaxDbiP0C7A3
PRIDEiP0C7A3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095345; ENSMUSP00000092988; ENSMUSG00000071113
GeneIDi234155
KEGGimmu:234155
UCSCiuc012gch.1 mouse

Organism-specific databases

CTDi619373
MGIiMGI:2685017 Mboat4

Phylogenomic databases

eggNOGiKOG2704 Eukaryota
COG5202 LUCA
GeneTreeiENSGT00550000074565
HOGENOMiHOG000113492
HOVERGENiHBG108120
InParanoidiP0C7A3
OMAiKWNQSTA
OrthoDBiEOG091G07L0
PhylomeDBiP0C7A3
TreeFamiTF314906

Enzyme and pathway databases

ReactomeiR-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin

Miscellaneous databases

PROiPR:P0C7A3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071113 Expressed in 108 organ(s), highest expression level in bone marrow
GenevisibleiP0C7A3 MM

Family and domain databases

InterProiView protein in InterPro
IPR004299 MBOAT_fam
PfamiView protein in Pfam
PF03062 MBOAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMBOA4_MOUSE
AccessioniPrimary (citable) accession number: P0C7A3
Secondary accession number(s): B1Q004, B2RWL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: November 7, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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