UniProtKB - P0C6Y2 (R1AB_IBVBC)
Protein
Replicase polyprotein 1ab
Gene
rep
Organism
Avian infectious bronchitis virus (strain Beaudette CK) (IBV)
Status
Functioni
The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).By similarity
Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.PROSITE-ProRule annotation
The peptide p16 might be involved in the EGF signaling pathway.By similarity
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).By similarity
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.By similarity
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1274 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1437 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 2820 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 2922 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Metal bindingi | 4873 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4876 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4884 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4887 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4894 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4897 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4901 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4907 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4918 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4923 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4940 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4943 | Zinc 3PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1353 – 1390 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 3858 – 3878 | By similarityAdd BLAST | 21 | |
Zinc fingeri | 3904 – 3917 | By similarityAdd BLAST | 14 | |
Nucleotide bindingi | 5150 – 5157 | ATPBy similarity | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cysteine-type endopeptidase activity Source: InterPro
- DNA helicase activity Source: UniProtKB-EC
- endonuclease activity Source: UniProtKB-KW
- exonuclease activity Source: UniProtKB-KW
- methyltransferase activity Source: UniProtKB-KW
- omega peptidase activity Source: InterPro
- RNA binding Source: UniProtKB-KW
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- RNA helicase activity Source: UniProtKB-EC
- zinc ion binding Source: InterPro
GO - Biological processi
- induction by virus of host autophagy Source: UniProtKB-KW
- methylation Source: UniProtKB-KW
- transcription, DNA-templated Source: InterPro
- viral protein processing Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Endonuclease, Exonuclease, Helicase, Hydrolase, Methyltransferase, Nuclease, Nucleotidyltransferase, Protease, RNA-binding, RNA-directed RNA polymerase, Thiol protease, Transferase |
Biological process | Activation of host autophagy by virus, Host-virus interaction, Viral RNA replication |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1abShort name: pp1ab Alternative name(s): ORF1ab polyprotein Cleaved into the following 14 chains: Alternative name(s): p87 Alternative name(s): Papain-like proteinase Short name: PL-PRO p195 Alternative name(s): Peptide HD2 p41 Alternative name(s): M-PRO nsp5 p33 Alternative name(s): p34 Alternative name(s): p9 Alternative name(s): p24 Alternative name(s): p10 Alternative name(s): Growth factor-like peptide Short name: GFL p16 Alternative name(s): nsp12 p100 Alternative name(s): nsp13 p68 Alternative name(s): nsp14 p58 Alternative name(s): NendoU nsp15 p39 Alternative name(s): nsp16 p35 |
Gene namesi | Name:rep ORF Names:1a-1b |
Organismi | Avian infectious bronchitis virus (strain Beaudette CK) (IBV) |
Taxonomic identifieri | 160235 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Gammacoronavirus › Igacovirus › |
Virus hosti | Gallus gallus (Chicken) [TaxID: 9031] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- Host endoplasmic reticulum-Golgi intermediate compartment Curated Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1721 – 1741 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1751 – 1771 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1844 – 1864 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1869 – 1888 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 2281 – 2301 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2560 – 2580 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2589 – 2609 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2612 – 2632 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2644 – 2664 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3101 – 3121 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3122 – 3142 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3152 – 3172 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3189 – 3209 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3220 – 3240 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3258 – 3278 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3297 – 3317 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
- host cell membrane Source: UniProtKB-SubCell
- host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000037418 | 1 – 673 | Non-structural protein 2By similarityAdd BLAST | 673 | |
ChainiPRO_0000037419 | 674 – 2265 | Non-structural protein 3By similarityAdd BLAST | 1592 | |
ChainiPRO_0000037420 | 2266 – 2779 | Non-structural protein 4By similarityAdd BLAST | 514 | |
ChainiPRO_0000037421 | 2780 – 3086 | 3C-like proteinaseBy similarityAdd BLAST | 307 | |
ChainiPRO_0000037422 | 3087 – 3379 | Non-structural protein 6By similarityAdd BLAST | 293 | |
ChainiPRO_0000037423 | 3380 – 3462 | Non-structural protein 7By similarityAdd BLAST | 83 | |
ChainiPRO_0000037424 | 3463 – 3672 | Non-structural protein 8By similarityAdd BLAST | 210 | |
ChainiPRO_0000037425 | 3673 – 3783 | Non-structural protein 9By similarityAdd BLAST | 111 | |
ChainiPRO_0000037426 | 3784 – 3928 | Non-structural protein 10By similarityAdd BLAST | 145 | |
ChainiPRO_0000037427 | 3929 – 4868 | RNA-directed RNA polymeraseBy similarityAdd BLAST | 940 | |
ChainiPRO_0000037428 | 4869 – 5468 | HelicaseBy similarityAdd BLAST | 600 | |
ChainiPRO_0000037429 | 5469 – 5989 | ExoribonucleaseBy similarityAdd BLAST | 521 | |
ChainiPRO_0000037430 | 5990 – 6327 | Uridylate-specific endoribonucleaseBy similarityAdd BLAST | 338 | |
ChainiPRO_0000037431 | 6328 – 6629 | Putative 2'-O-methyl transferaseBy similarityAdd BLAST | 302 |
Post-translational modificationi
Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 673 – 674 | Cleavage; by PL-PROBy similarity | 2 | |
Sitei | 2265 – 2266 | Cleavage; by PL-PROBy similarity | 2 | |
Sitei | 2779 – 2780 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3086 – 3087 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3379 – 3380 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3462 – 3463 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3672 – 3673 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3783 – 3784 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3928 – 3929 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 4868 – 4869 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 5468 – 5469 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 5989 – 5990 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 6327 – 6328 | Cleavage; by 3CL-PROBy similarity | 2 |
Proteomic databases
PRIDEi | P0C6Y2 |
Interactioni
Subunit structurei
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1003 – 1179 | MacroPROSITE-ProRule annotationAdd BLAST | 177 | |
Domaini | 1236 – 1497 | Peptidase C16PROSITE-ProRule annotationAdd BLAST | 262 | |
Domaini | 2780 – 3086 | Peptidase C30PROSITE-ProRule annotationAdd BLAST | 307 | |
Domaini | 4548 – 4710 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 163 | |
Domaini | 4869 – 4952 | CV ZBDPROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 5125 – 5305 | (+)RNA virus helicase ATP-bindingAdd BLAST | 181 | |
Domaini | 5306 – 5477 | (+)RNA virus helicase C-terminalAdd BLAST | 172 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1721 – 1888 | HD1Add BLAST | 168 | |
Regioni | 2281 – 2664 | HD2Add BLAST | 384 | |
Regioni | 2885 – 3317 | HD3Add BLAST | 433 |
Domaini
The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.
Sequence similaritiesi
Belongs to the coronaviruses polyprotein 1ab family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1353 – 1390 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 3858 – 3878 | By similarityAdd BLAST | 21 | |
Zinc fingeri | 3904 – 3917 | By similarityAdd BLAST | 14 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
CDDi | cd18808, SF1_C_Upf1, 1 hit |
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.370, 1 hit 2.20.25.360, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.40.220.10, 1 hit 3.40.50.11580, 1 hit 3.90.70.90, 1 hit |
InterProi | View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR003593, AAA+_ATPase IPR043609, CoV_NSP15_C IPR043608, CoV_NSP15_M IPR043606, CoV_NSP15_N IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR027352, CV_ZBD IPR043502, DNA/RNA_pol_sf IPR041679, DNA2/NAM7-like_C IPR037227, EndoU-like IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR009466, NSP14_CoV IPR043174, NSP15_middle_sf IPR042515, NSP15_N_CoV IPR009461, NSP16_CoV-like IPR040795, Nsp1a_N IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR027417, P-loop_NTPase IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043503, PLpro_palm_finger_dom_CoV IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus IPR009469, RNA_pol_N_coronovir IPR018995, RNA_synth_NSP10_CoV IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF06471, CoV_Methyltr_1, 1 hit PF06460, CoV_Methyltr_2, 1 hit PF09401, CoV_NSP10, 1 hit PF19215, CoV_NSP15_C, 1 hit PF19216, CoV_NSP15_M, 1 hit PF19219, CoV_NSP15_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF06478, CoV_RPol_N, 1 hit PF01661, Macro, 1 hit PF17896, Nsp2a_N, 1 hit PF05409, Peptidase_C30, 1 hit PF00680, RdRP_1, 1 hit PF01443, Viral_helicase1, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit SM00382, AAA, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF142877, SSF142877, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF52949, SSF52949, 1 hit SSF53335, SSF53335, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51653, CV_ZBD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform Replicase polyprotein 1ab (identifier: P0C6Y2-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASSLKQGVS PKPRDVILVS KDIPEQLCDA LFFYTSHNPK DYADAFAVRQ
60 70 80 90 100
KFDRSLQTGK QFKFETVCGL FLLKGVDKIT PGVPAKVLKA TSKLADLEDI
110 120 130 140 150
FGVSPLARKY RELLKTACQW SLTVEALDVR AQTLDEIFDP TEILWLQVAA
160 170 180 190 200
KIHVSSMAMR RLVGEVTAKV MDALGSNLSA LFQIVKQQIA RIFQKALAIF
210 220 230 240 250
ENVNELPQRI AALKMAFAKC ARSITVVVVE RTLVVKEFAG TCLASINGAV
260 270 280 290 300
AKFFEELPNG FMGSKIFTTL AFFKEAAVRV VENIPNAPRG TKGFEVVGNA
310 320 330 340 350
KGTQVVVRGM RNDLTLLDQK ADIPVEPEGW SAILDGHLCY VFRSGDRFYA
360 370 380 390 400
APLSGNFALS DVHCCERVVC LSDGVTPEIN DGLILAAIYS SFSVSELVTA
410 420 430 440 450
LKKGEPFKFL GHKFVYAKDA AVSFTLAKAA TIADVLRLFQ SARVIAEDVW
460 470 480 490 500
SSFTEKSFEF WKLAYGKVRN LEEFVKTYVC KAQMSIVILA AVLGEDIWHL
510 520 530 540 550
VSQVIYKLGV LFTKVVDFCD KHWKGFCVQL KRAKLIVTET FCVLKGVAQH
560 570 580 590 600
CFQLLLDAIH SLYKSFKKCA LGRIHGDLLF WKGGVHKIVQ DGDEIWFDAI
610 620 630 640 650
DSVDVEDLGV VQEKSIDFEV CDDVTLPENQ PGHMVQIEDD GKNYMFFRFK
660 670 680 690 700
KDENIYYTPM SQLGAINVVC KAGGKTVTFG ETTVQEIPPP DVVPIKVSIE
710 720 730 740 750
CCGEPWNTIF KKAYKEPIEV DTDLTVEQLL SVIYEKMCDD LKLFPEAPEP
760 770 780 790 800
PPFENVALVD KNGKDLDCIK SCHLIYRDYE SDDDIEEEDA EECDTDSGEA
810 820 830 840 850
EECDTNSECE EEDEDTKVLA LIQDPASIKY PLPLDEDYSV YNGCIVHKDA
860 870 880 890 900
LDVVNLPSGE ETFVVNNCFE GAVKPLPQKV VDVLGDWGEA VDAQEQLCQQ
910 920 930 940 950
EPLQHTFEEP VENSTGSSKT MTEQVVVEDQ ELPVVEQDQD VVVYTPTDLE
960 970 980 990 1000
VAKETAEEVD EFILIFAVPK EEVVSQKDGA QIKQEPIQVV KPQREKKAKK
1010 1020 1030 1040 1050
FKVKPATCEK PKFLEYKTCV GDLTVVIAKA LDEFKEFCIV NAANEHMTHG
1060 1070 1080 1090 1100
SGVAKAIADF CGLDFVEYCE DYVKKHGPQQ RLVTPSFVKG IQCVNNVVGP
1110 1120 1130 1140 1150
RHGDNNLHEK LVAAYKNVLV DGVVNYVVPV LSLGIFGVDF KMSIDAMREA
1160 1170 1180 1190 1200
FEGCTIRVLL FSLSQEHIDY FDVTCKQKTI YLTEDGVKYR SIVLKPGDSL
1210 1220 1230 1240 1250
GQFGQVYAKN KIVFTADDVE DKEILYVPTT DKSILEYYGL DAQKYVIYLQ
1260 1270 1280 1290 1300
TLAQKWNVQY RDNFLILEWR DGNCWISSAI VLLQAAKIRF KGFLTEAWAK
1310 1320 1330 1340 1350
LLGGDPTDFV AWCYASCTAK VGDFSDANWL LANLAEHFDA DYTNAFLKKR
1360 1370 1380 1390 1400
VSCNCGIKSY ELRGLEACIQ PVRATNLLHF KTQYSNCPTC GANNTDEVIE
1410 1420 1430 1440 1450
ASLPYLLLFA TDGPATVDCD EDAVGTVVFV GSTNSGHCYT QAAGQAFDNL
1460 1470 1480 1490 1500
AKDRKFGKKS PYITAMYTRF AFKNETSLPV AKQSKGKSKS VKEDVSNLAT
1510 1520 1530 1540 1550
SSKASFDNLT DFEQWYDSNI YESLKVQESP DNFDKYVSFT TKEDSKLPLT
1560 1570 1580 1590 1600
LKVRGIKSVV DFRSKDGFIY KLTPDTDENS KAPVYYPVLD AISLKAIWVE
1610 1620 1630 1640 1650
GNANFVVGHP NYYSKSLHIP TFWENAENFV KMGDKIGGVT MGLWRAEHLN
1660 1670 1680 1690 1700
KPNLERIFNI AKKAIVGSSV VTTQCGKLIG KAATFIADKV GGGVVRNITD
1710 1720 1730 1740 1750
SIKGLCGITR GHFERKMSPQ FLKTLMFFLF YFLKASVKSV VASYKTVLCK
1760 1770 1780 1790 1800
VVLATLLIVW FVYTSNPVMF TGIRVLDFLF EGSLCGPYKD YGKDSFDVLR
1810 1820 1830 1840 1850
YCADDFICRV CLHDKDSLHL YKHAYSVEQV YKDAASGFIF NWNWLYLVFL
1860 1870 1880 1890 1900
ILFVKPVAGF VIICYCVKYL VLNSTVLQTG VCFLDWFVQT VFSHFNFMGA
1910 1920 1930 1940 1950
GFYFWLFYKI YIQVHHILYC KDVTCEVCKR VARSNRQEVS VVVGGRKQIV
1960 1970 1980 1990 2000
HVYTNSGYNF CKRHNWYCRN CDDYGHQNTF MSPEVAGELS EKLKRHVKPT
2010 2020 2030 2040 2050
AYAYHVVDEA CLVDDFVNLK YKAATPGKDS ASSAVKCFSV TDFLKKAVFL
2060 2070 2080 2090 2100
KEALKCEQIS NDGFIVCNTQ SAHALEEAKN AAIYYAQYLC KPILILDQAL
2110 2120 2130 2140 2150
YEQLVVEPVS KSVIDKVCSI LSSIISVDTA ALNYKAGTLR DALLSITKDE
2160 2170 2180 2190 2200
EAVDMAIFCH NHDVDYTGDG FTNVIPSYGI DTGKLTPRDR GFLINADASI
2210 2220 2230 2240 2250
ANLRVKNAPP VVWKFSELIK LSDSCLKYLI SATVKSGVRF FITKSGAKQV
2260 2270 2280 2290 2300
IACHTQKLLV EKKAGGIVSG TFKCFKSYFK WLLIFYILFT ACCSGYYYME
2310 2320 2330 2340 2350
VSKSFVHPMY DVNSTLHVEG FKVIDKGVLR EIVPEDTCFS NKFVNFDAFW
2360 2370 2380 2390 2400
GRPYDNSRNC PIVTAVIDGD GTVATGVPGF VSWVMDGVMF IHMTQTERKP
2410 2420 2430 2440 2450
WYIPTWFNRE IVGYTQDSII TEGSFYTSIA LFSARCLYLT ASNTPQLYCF
2460 2470 2480 2490 2500
NGDNDAPGAL PFGSIIPHRV YFQPNGVRLI VPQQILHTPY VVKFVSDSYC
2510 2520 2530 2540 2550
RGSVCEYTRP GYCVSLNPQW VLFNDEYTSK PGVFCGSTVR ELMFSMVSTF
2560 2570 2580 2590 2600
FTGVNPNIYM QLATMFLILV VVVLIFAMVI KFQGVFKAYA TTVFITMLVW
2610 2620 2630 2640 2650
VINAFILCVH SYNSVLAVIL LVLYCYASLV TSRNTVIIMH CWLVFTFGLI
2660 2670 2680 2690 2700
VPTWLACCYL GFIIYMYTPL FLWCYGTTKN TRKLYDGNEF VGNYDLAAKS
2710 2720 2730 2740 2750
TFVIRGSEFV KLTNEIGDKF EAYLSAYARL KYYSGTGSEQ DYLQACRAWL
2760 2770 2780 2790 2800
AYALDQYRNS GVEIVYTPPR YSIGVSRLQS GFKKLVSPSS AVEKCIVSVS
2810 2820 2830 2840 2850
YRGNNLNGLW LGDTIYCPRH VLGKFSGDQW NDVLNLANNH EFEVTTQHGV
2860 2870 2880 2890 2900
TLNVVSRRLK GAVLILQTAV ANAETPKYKF IKANCGDSFT IACAYGGTVV
2910 2920 2930 2940 2950
GLYPVTMRSN GTIRASFLAG ACGSVGFNIE KGVVNFFYMH HLELPNALHT
2960 2970 2980 2990 3000
GTDLMGEFYG GYVDEEVAQR VPPDNLVTNN IVAWLYAAII SVKESSFSLP
3010 3020 3030 3040 3050
KWLESTTVSV DDYNKWAGDN GFTPFSTSTA ITKLSAITGV DVCKLLRTIM
3060 3070 3080 3090 3100
VKNSQWGGDP ILGQYNFEDE LTPESVFNQI GGVRLQSSFV RKATSWFWSR
3110 3120 3130 3140 3150
CVLACFLFVL CAIVLFTAVP LKFYVYAAVI LLMAVLFISF TVKHVMAYMD
3160 3170 3180 3190 3200
TFLLPTLITV IIGVCAEVPF IYNTLISQVV IFLSQWYDPV VFDTMVPWMF
3210 3220 3230 3240 3250
LPLVLYTAFK CVQGCYMNSF NTSLLMLYQF VKLGFVIYTS SNTLTAYTEG
3260 3270 3280 3290 3300
NWELFFELVH TTVLANVSSN SLIGLFVFKC AKWMLYYCNA TYLNNYVLMA
3310 3320 3330 3340 3350
VMVNCIGWLC TCYFGLYWWV NKVFGLTLGK YNFKVSVDQY RYMCLHKINP
3360 3370 3380 3390 3400
PKTVWEVFST NILIQGIGGD RVLPIATVQA KLSDVKCTTV VLMQLLTKLN
3410 3420 3430 3440 3450
VEANSKMHVY LVELHNKILA SDDVGECMDN LLGMLITLFC IDSTIDLSEY
3460 3470 3480 3490 3500
CDDILKRSTV LQSVTQEFSH IPSYAEYERA KNLYEKVLVD SKNGGVTQQE
3510 3520 3530 3540 3550
LAAYRKAANI AKSVFDRDLA VQKKLDSMAE RAMTTMYKEA RVTDRRAKLV
3560 3570 3580 3590 3600
SSLHALLFSM LKKIDSEKLN VLFDQASSGV VPLATVPIVC SNKLTLVIPD
3610 3620 3630 3640 3650
PETWVKCVEG VHVTYSTVVW NIDTVIDADG TELHPTSTGS GLTYCISGAN
3660 3670 3680 3690 3700
IAWPLKVNLT RNGHNKVDVV LQNNELMPHG VKTKACVAGV DQAHCSVESK
3710 3720 3730 3740 3750
CYYTNISGNS VVAAITSSNP NLKVASFLNE AGNQIYVDLD PPCKFGMKVG
3760 3770 3780 3790 3800
VKVEVVYLYF IKNTRSIVRG MVLGAISNVV VLQSKGHETE EVDAVGILSL
3810 3820 3830 3840 3850
CSFAVDPADT YCKYVAAGNQ PLGNCVKMLT VHNGSGFAIT SKPSPTPDQD
3860 3870 3880 3890 3900
SYGGASVCLY CRAHIAHPGS VGNLDGRCQF KGSFVQIPTT EKDPVGFCLR
3910 3920 3930 3940 3950
NKVCTVCQCW IGYGCQCDSL RQPKSSVQSV AGASDFDKNY LNRVRGSSEA
3960 3970 3980 3990 4000
RLIPLASGCD PDVVKRAFDV CNKESAGMFQ NLKRNCARFQ ELRDTEDGNL
4010 4020 4030 4040 4050
EYLDSYFVVK QTTPSNYEHE KSCYEDLKSE VTADHDFFVF NKNIYNISRQ
4060 4070 4080 4090 4100
RLTKYTMMDF CYALRHFDPK DCEVLKEILV TYGCIEDYHP KWFEENKDWY
4110 4120 4130 4140 4150
DPIENSKYYV MLAKMGPIVR RALLNAIEFG NLMVEKGYVG VITLDNQDLN
4160 4170 4180 4190 4200
GKFYDFGDFQ KTAPGAGVPV FDTYYSYMMP IIAMTDALAP ERYFEYDVHK
4210 4220 4230 4240 4250
GYKSYDLLKY DYTEEKQELF QKYFKYWDQE YHPNCRDCSD DRCLIHCANF
4260 4270 4280 4290 4300
NILFSTLIPQ TSFGNLCRKV FVDGVPFIAT CGYHSKELGV IMNQDNTMSF
4310 4320 4330 4340 4350
SKMGLSQLMQ FVGDPALLVG TSNNLVDLRT SCFSVCALTS GITHQTVKPG
4360 4370 4380 4390 4400
HFNKDFYDFA EKAGMFKEGS SIPLKHFFYP QTGNAAINDY DYYRYNRPTM
4410 4420 4430 4440 4450
FDICQLLFCL EVTSKYFECY EGGCIPASQV VVNNLDKSAG YPFNKFGKAR
4460 4470 4480 4490 4500
LYYEMSLEEQ DQLFEITKKN VLPTITQMNL KYAISAKNRA RTVAGVSILS
4510 4520 4530 4540 4550
TMTNRQFHQK ILKSIVNTRN ASVVIGTTKF YGGWDNMLRN LIQGVEDPIL
4560 4570 4580 4590 4600
MGWDYPKCDR AMPNLLRIAA SLVLARKHTN CCSWSERIYR LYNECAQVLS
4610 4620 4630 4640 4650
ETVLATGGIY VKPGGTSSGD ATTAYANSVF NIIQATSANV ARLLSVITRD
4660 4670 4680 4690 4700
IVYDNIKSLQ YELYQQVYRR VNFDPAFVEK FYSYLCKNFS LMILSDDGVV
4710 4720 4730 4740 4750
CYNNTLAKQG LVADISGFRE VLYYQNNVFM ADSKCWVEPD LEKGPHEFCS
4760 4770 4780 4790 4800
QHTMLVEVDG EPKYLPYPDP SRILGACVFV DDVDKTEPVA VMERYIALAI
4810 4820 4830 4840 4850
DAYPLVHHEN EEYKKVFFVL LAYIRKLYQE LSQNMLMDYS FVMDIDKGSK
4860 4870 4880 4890 4900
FWEQEFYENM YRAPTTLQSC GVCVVCNSQT ILRCGNCIRK PFLCCKCCYD
4910 4920 4930 4940 4950
HVMHTDHKNV LSINPYICSQ LGCGEADVTK LYLGGMSYFC GNHKPKLSIP
4960 4970 4980 4990 5000
LVSNGTVFGI YRANCAGSEN VDDFNQLATT NWSIVEPYIL ANRCSDSLRR
5010 5020 5030 5040 5050
FAAETVKATE ELHKQQFASA EVREVFSDRE LILSWEPGKT RPPLNRNYVF
5060 5070 5080 5090 5100
TGYHFTRTSK VQLGDFTFEK GEGKDVVYYK ATSTAKLSVG DIFVLTSHNV
5110 5120 5130 5140 5150
VSLVAPTLCP QQTFSRFVNL RPNVMVPECF VNNIPLYHLV GKQKRTTVQG
5160 5170 5180 5190 5200
PPGSGKSHFA IGLAVYFSSA RVVFTACSHA AVDALCEKAF KFLKVDDCTR
5210 5220 5230 5240 5250
IVPQRTTVDC FSKFKANDTG KKYIFSTINA LPEVSCDILL VDEVSMLTNY
5260 5270 5280 5290 5300
ELSFINGKIN YQYVVYVGDP AQLPAPRTLL NGSLSPKDYN VVTNLMVCVK
5310 5320 5330 5340 5350
PDIFLAKCYR CPKEIVDTVS TLVYDGKFIA NNPESRECFK VIVNNGNSDV
5360 5370 5380 5390 5400
GHESGSAYNT TQLEFVKDFV CRNKQWREAI FISPYNAMNQ RAYRMLGLNV
5410 5420 5430 5440 5450
QTVDSSQGSE YDYVIFCVTA DSQHALNINR FNVALTRAKR GILVVMRQRD
5460 5470 5480 5490 5500
ELYSALKFTE LDSETSLQGT GLFKICNKEF SGVHPAYAVT TKALAATYKV
5510 5520 5530 5540 5550
NDELAALVNV EAGSEITYKH LISLLGFKMS VNVEGCHNMF ITRDEAIRNV
5560 5570 5580 5590 5600
RGWVGFDVEA THACGTNIGT NLPFQVGFST GADFVVTPEG LVDTSIGNNF
5610 5620 5630 5640 5650
EPVNSKAPPG EQFNHLRVLF KSAKPWHVIR PRIVQMLADN LCNVSDCVVF
5660 5670 5680 5690 5700
VTWCHGLELT TLRYFVKIGK EQVCSCGSRA TTFNSHTQAY ACWKHCLGFD
5710 5720 5730 5740 5750
FVYNPLLVDI QQWGYSGNLQ FNHDLHCNVH GHAHVASVDA IMTRCLAINN
5760 5770 5780 5790 5800
AFCQDVNWDL TYPHIANEDE VNSSCRYLQR MYLNACVDAL KVNVVYDIGN
5810 5820 5830 5840 5850
PKGIKCVRRG DVNFRFYDKN PIVRNVKQFE YDYNQHKDKF ADGLCMFWNC
5860 5870 5880 5890 5900
NVDCYPDNSL VCRYDTRNLS VFNLPGCNGG SLYVNKHAFY TPKFDRISFR
5910 5920 5930 5940 5950
NLKAMPFFFY DSSPCETIQV DGVAQDLVSL ATKDCITKCN IGGAVCKKHA
5960 5970 5980 5990 6000
QMYAEFVTSY NAAVTAGFTF WVTNKLNPYN LWKSFSALQS IDNIAYNMYK
6010 6020 6030 6040 6050
GGHYDAIAGE MPTVITGDKV FVIDQGVEKA VFVNQTTLPT SVAFELYAKR
6060 6070 6080 6090 6100
NIRTLPNNRI LKGLGVDVTN GFVIWDYANQ TPLYRNTVKV CAYTDIEPNG
6110 6120 6130 6140 6150
LVVLYDDRYG DYQSFLAADN AVLVSTQCYK RYSYVEIPSN LLVQNGMPLK
6160 6170 6180 6190 6200
DGANLYVYKR VNGAFVTLPN TINTQGRSYE TFEPRSDIER DFLAMSEESF
6210 6220 6230 6240 6250
VERYGKDLGL QHILYGEVDK PQLGGLHTVI GMYRLLRANK LNAKSVTNSD
6260 6270 6280 6290 6300
SDVMQNYFVL SDNGSYKQVC TVVDLLLDDF LELLRNILKE YGTNKSKVVT
6310 6320 6330 6340 6350
VSIDYHSINF MTWFEDGSIK TCYPQLQSAW TCGYNMPELY KVQNCVMEPC
6360 6370 6380 6390 6400
NIPNYGVGIT LPSGILMNVA KYTQLCQYLL KTTICVPHNM RVMHFGAGSD
6410 6420 6430 6440 6450
KGVAPGSTVL KQWLPEGTLL VDNDIVDYVS DAHVSVLSDC NKYNTEHKFD
6460 6470 6480 6490 6500
LVISDMYTDN DSKRKHEGVI ANNGNDDVFI YLSSFLRNNL ALGGSFAVKV
6510 6520 6530 6540 6550
TETSWHEVLY DIAQDCAWWT MFCTAVNASS SEAFLIGVNY LGASEKVKVS
6560 6570 6580 6590 6600
GKTLHANYIF WRNCNYLQTS AYSIFDVAKF DLRLKATPVV NLKTEQKTDL
6610 6620
VFNLIKCGKL LVRDVGNTSF TSDSFVCTM
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: P0C6V4-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein
The sequence of this isoform can be found in the external entry P0C6V4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence cautioni
The sequence CAC39112 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ311317 Genomic RNA Translation: CAC39112.1 Sequence problems. |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ311317 Genomic RNA Translation: CAC39112.1 Sequence problems. |
3D structure databases
SMRi | P0C6Y2 |
ModBasei | Search... |
Proteomic databases
PRIDEi | P0C6Y2 |
Family and domain databases
CDDi | cd18808, SF1_C_Upf1, 1 hit |
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.370, 1 hit 2.20.25.360, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.40.220.10, 1 hit 3.40.50.11580, 1 hit 3.90.70.90, 1 hit |
InterProi | View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR003593, AAA+_ATPase IPR043609, CoV_NSP15_C IPR043608, CoV_NSP15_M IPR043606, CoV_NSP15_N IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR027352, CV_ZBD IPR043502, DNA/RNA_pol_sf IPR041679, DNA2/NAM7-like_C IPR037227, EndoU-like IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR009466, NSP14_CoV IPR043174, NSP15_middle_sf IPR042515, NSP15_N_CoV IPR009461, NSP16_CoV-like IPR040795, Nsp1a_N IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR027417, P-loop_NTPase IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043503, PLpro_palm_finger_dom_CoV IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus IPR009469, RNA_pol_N_coronovir IPR018995, RNA_synth_NSP10_CoV IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF06471, CoV_Methyltr_1, 1 hit PF06460, CoV_Methyltr_2, 1 hit PF09401, CoV_NSP10, 1 hit PF19215, CoV_NSP15_C, 1 hit PF19216, CoV_NSP15_M, 1 hit PF19219, CoV_NSP15_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF06478, CoV_RPol_N, 1 hit PF01661, Macro, 1 hit PF17896, Nsp2a_N, 1 hit PF05409, Peptidase_C30, 1 hit PF00680, RdRP_1, 1 hit PF01443, Viral_helicase1, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit SM00382, AAA, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF142877, SSF142877, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF52949, SSF52949, 1 hit SSF53335, SSF53335, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51653, CV_ZBD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | R1AB_IBVBC | |
Accessioni | P0C6Y2Primary (citable) accession number: P0C6Y2 Secondary accession number(s): Q91QT2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | December 2, 2020 | |
This is version 79 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |