rep

Functionⁱ

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.By similarity

Host translation inhibitor nsp1:

Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.By similarity

Non-structural protein 2:

May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.By similarity

Papain-like proteinase:

Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.By similarity

Non-structural protein 4:

Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.By similarity

3C-like proteinase:

Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).PROSITE-ProRule annotationBy similarity

Non-structural protein 6:

Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.By similarity

Non-structural protein 7:

Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.By similarity

Non-structural protein 8:

Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.By similarity

Non-structural protein 9:

May participate in viral replication by acting as a ssRNA-binding protein.By similarity

Non-structural protein 10:

Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.By similarity

RNA-directed RNA polymerase:

Responsible for replication and transcription of the viral RNA genome.By similarity

Helicase:

Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.By similarity

Guanine-N7 methyltransferase:

Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.By similarity

Uridylate-specific endoribonuclease:

Mn²⁺-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity

2'-O-methyltransferase: Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.By similarity

Catalytic activityⁱ

TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. EC:3.4.22.69
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	1120	For PL1-PRO activityPROSITE-ProRule annotation	1
Active siteⁱ	1271	For PL1-PRO activityPROSITE-ProRule annotation	1
Active siteⁱ	1715	For PL2-PRO activityPROSITE-ProRule annotation	1
Active siteⁱ	1872	For PL2-PRO activityPROSITE-ProRule annotation	1
Active siteⁱ	3377	For 3CL-PRO activityPROSITE-ProRule annotation	1
Active siteⁱ	3481	For 3CL-PRO activityPROSITE-ProRule annotation	1
Metal bindingⁱ	5393	Zinc 1PROSITE-ProRule annotation	1
Metal bindingⁱ	5396	Zinc 1PROSITE-ProRule annotation	1
Metal bindingⁱ	5404	Zinc 2PROSITE-ProRule annotation	1
Metal bindingⁱ	5407	Zinc 1PROSITE-ProRule annotation	1
Metal bindingⁱ	5414	Zinc 1PROSITE-ProRule annotation	1
Metal bindingⁱ	5417	Zinc 2PROSITE-ProRule annotation	1
Metal bindingⁱ	5421	Zinc 2PROSITE-ProRule annotation	1
Metal bindingⁱ	5427	Zinc 2PROSITE-ProRule annotation	1
Metal bindingⁱ	5438	Zinc 3PROSITE-ProRule annotation	1
Metal bindingⁱ	5443	Zinc 3PROSITE-ProRule annotation	1
Metal bindingⁱ	5460	Zinc 3PROSITE-ProRule annotation	1
Metal bindingⁱ	5463	Zinc 3PROSITE-ProRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	1197 – 1225	C4-type 1PROSITE-ProRule annotationAdd BLAST	29
Zinc fingerⁱ	1793 – 1829	C4-type 2PROSITE-ProRule annotationAdd BLAST	37
Zinc fingerⁱ	4397 – 4413	By similarityAdd BLAST	17
Zinc fingerⁱ	4439 – 4452	By similarityAdd BLAST	14
Nucleotide bindingⁱ	5669 – 5676	ATPBy similarity	8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
cysteine-type endopeptidase activity Source: InterPro
DNA helicase activity Source: UniProtKB-EC
endonuclease activity Source: UniProtKB-KW
exonuclease activity Source: UniProtKB-KW
methyltransferase activity Source: UniProtKB-KW
RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
RNA helicase activity Source: UniProtKB-EC
single-stranded RNA binding Source: InterPro
thiol-dependent ubiquitin-specific protease activity Source: UniProtKB-EC
zinc ion binding Source: InterPro

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

induction by virus of catabolism of host mRNA Source: UniProtKB-KW
induction by virus of host autophagy Source: UniProtKB-KW
methylation Source: UniProtKB-KW
modulation by virus of host protein ubiquitination Source: UniProtKB-KW
suppression by virus of host ISG15 activity Source: UniProtKB-KW
suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
transcription, DNA-templated Source: InterPro
viral protein processing Source: InterPro
viral RNA genome replication Source: InterPro

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Endonuclease, Exonuclease, Helicase, Hydrolase, Methyltransferase, Nuclease, Nucleotidyltransferase, Protease, RNA-binding, RNA-directed RNA polymerase, Thiol protease, Transferase
Biological process	Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Inhibition of host NF-kappa-B by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication
Ligand	ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Replicase polyprotein 1ab Short name: pp1ab Alternative name(s): ORF1ab polyprotein Cleaved into the following 15 chains: Host translation inhibitor nsp1 Short name: nsp1 Alternative name(s): p28 Non-structural protein 2 Short name: nsp2 Alternative name(s): p65 Papain-like proteinase (EC:3.4.19.12, EC:3.4.22.69) Short name: PL-PRO Alternative name(s): Non-structural protein 3 Short name: nsp3 p210 Non-structural protein 4 Short name: nsp4 Alternative name(s): Peptide HD2 p44 3C-like proteinase (EC:3.4.22.-) Short name: 3CL-PRO Short name: 3CLp Alternative name(s): M-PRO nsp5 p27 Non-structural protein 6 Short name: nsp6 Non-structural protein 7 Short name: nsp7 Alternative name(s): p10 Non-structural protein 8 Short name: nsp8 Alternative name(s): p22 Non-structural protein 9 Short name: nsp9 Alternative name(s): p12 Non-structural protein 10 Short name: nsp10 Alternative name(s): Growth factor-like peptide Short name: GFL p15 RNA-directed RNA polymerase (EC:2.7.7.48) Short name: Pol Short name: RdRp Alternative name(s): nsp12 p100 Helicase (EC:3.6.4.12, EC:3.6.4.13) Short name: Hel Alternative name(s): nsp13 p67 Guanine-N7 methyltransferase (EC:2.1.1.-, EC:3.1.13.-) Short name: ExoN Alternative name(s): nsp14 Uridylate-specific endoribonuclease (EC:3.1.-.-) Alternative name(s): NendoU nsp15 p35 2'-O-methyl transferase (EC:2.1.1.-) Alternative name(s): nsp16
Gene namesⁱ	Name:rep ORF Names:1a-1b
Organismⁱ	Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Taxonomic identifierⁱ	11144 [NCBI]
Taxonomic lineageⁱ	Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Embecovirus › Murine coronavirus › Murine hepatitis virus
Virus hostⁱ	Mus musculus (Mouse) [TaxID: 10090]
Proteomesⁱ	UP000007193 Componentⁱ: Genome

Subcellular locationⁱ

Papain-like proteinase:

Host membrane Curated; Multi-pass membrane protein Curated

Non-structural protein 4:

Host membrane Curated; Multi-pass membrane protein Curated

Non-structural protein 6:

Host membrane Curated; Multi-pass membrane protein Curated

Non-structural protein 7:

host perinuclear region By similarity

Note:

nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

Non-structural protein 8:

host perinuclear region By similarity

Note:

nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

Non-structural protein 9:

host perinuclear region By similarity

Note:

nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

Non-structural protein 10:

host perinuclear region By similarity

Note:

nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

Helicase:

Host endoplasmic reticulum-Golgi intermediate compartment Curated

Note:

The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

Uridylate-specific endoribonuclease:

host perinuclear region By similarity

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	2228 – 2248	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	2289 – 2309	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	2320 – 2340	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	2403 – 2423	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	2445 – 2465	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	2846 – 2866	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3099 – 3119	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3121 – 3141	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3153 – 3173	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3180 – 3200	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3205 – 3225	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3648 – 3668	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3678 – 3698	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3705 – 3725	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3748 – 3768	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3775 – 3795	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3802 – 3822	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	3846 – 3866	HelicalSequence analysisAdd BLAST	21

GO - Cellular componentⁱ

host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
host cell membrane Source: UniProtKB-SubCell
host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
integral component of membrane Source: UniProtKB-KW

Complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host cytoplasm, Host membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	2835	F → A: No processing between p210 and peptide HD2. 1 Publication	1
Mutagenesisⁱ	2836	S → A: No effect. 1 Publication	1
Mutagenesisⁱ	2837	L → A: No effect. 1 Publication	1
Mutagenesisⁱ	2838	K → A: No effect. 1 Publication	1
Mutagenesisⁱ	2838	K → N: No effect. 1 Publication	1
Mutagenesisⁱ	2839	G → A: Partial processing between p210 and peptide HD2. 1 Publication	1
Mutagenesisⁱ	2839	G → N: No processing between p210 and peptide HD2. 1 Publication	1
Mutagenesisⁱ	2839	G → V: No processing between p210 and peptide HD2. 1 Publication	1
Mutagenesisⁱ	2840	G → A: No processing between p210 and peptide HD2. 1 Publication	1
Mutagenesisⁱ	2840	G → N: No processing between p210 and peptide HD2. 1 Publication	1
Mutagenesisⁱ	2840	G → V: No processing between p210 and peptide HD2. 1 Publication	1
Mutagenesisⁱ	2841	A → N: No effect. 1 Publication	1
Mutagenesisⁱ	2842	V → N or M: No effect. 1 Publication	1
Mutagenesisⁱ	2846	V → M: No effect. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
ChainⁱPRO_0000037354	1 – 247	Host translation inhibitor nsp1By similarityAdd BLAST	247
ChainⁱPRO_0000037355	248 – 832	Non-structural protein 2By similarityAdd BLAST	585
ChainⁱPRO_0000037356	833 – 2840	Papain-like proteinaseBy similarityAdd BLAST	2008
ChainⁱPRO_0000037357	2841 – 3336	Non-structural protein 4By similarityAdd BLAST	496
ChainⁱPRO_0000037358	3337 – 3639	3C-like proteinaseBy similarityAdd BLAST	303
ChainⁱPRO_0000037359	3640 – 3927	Non-structural protein 6By similarityAdd BLAST	288
ChainⁱPRO_0000037360	3928 – 4019	Non-structural protein 7By similarityAdd BLAST	92
ChainⁱPRO_0000037361	4020 – 4213	Non-structural protein 8By similarityAdd BLAST	194
ChainⁱPRO_0000037362	4214 – 4323	Non-structural protein 9By similarityAdd BLAST	110
ChainⁱPRO_0000037363	4324 – 4460	Non-structural protein 10By similarityAdd BLAST	137
ChainⁱPRO_0000037364	4461 – 5388	RNA-directed RNA polymeraseBy similarityAdd BLAST	928
ChainⁱPRO_0000037365	5389 – 5988	HelicaseBy similarityAdd BLAST	600
ChainⁱPRO_0000037366	5989 – 6507	Guanine-N7 methyltransferaseBy similarityAdd BLAST	519
ChainⁱPRO_0000037367	6508 – 6881	Uridylate-specific endoribonucleaseBy similarityAdd BLAST	374
ChainⁱPRO_0000037368	6882 – 7180	2'-O-methyl transferaseBy similarityAdd BLAST	299

Post-translational modificationⁱ

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Siteⁱ	247 – 248	Cleavage; by PL1-PROCurated	2
Siteⁱ	832 – 833	Cleavage; by PL1-PROCurated	2
Siteⁱ	2840 – 2841	Cleavage; by PL2-PROCurated	2
Siteⁱ	3336 – 3337	Cleavage; by 3CL-PROCurated	2
Siteⁱ	3639 – 3640	Cleavage; by 3CL-PROCurated	2
Siteⁱ	3927 – 3928	Cleavage; by 3CL-PROCurated	2
Siteⁱ	4019 – 4020	Cleavage; by 3CL-PROCurated	2
Siteⁱ	4213 – 4214	Cleavage; by 3CL-PROCurated	2
Siteⁱ	4323 – 4324	Cleavage; by 3CL-PROCurated	2
Siteⁱ	4460 – 4461	Cleavage; by 3CL-PROCurated	2
Siteⁱ	5388 – 5389	Cleavage; by 3CL-PROCurated	2
Siteⁱ	5988 – 5989	Cleavage; by 3CL-PROCurated	2
Siteⁱ	6507 – 6508	Cleavage; by 3CL-PROCurated	2
Siteⁱ	6881 – 6882	Cleavage; by 3CL-PROCurated	2

Proteomic databases

PRIDEⁱ	P0C6Y0

PRIDEⁱ

P0C6Y0

Interactionⁱ

Subunit structureⁱ

Nsp2 interacts with host PHB and PHB2. 3CL-PRO exists as monomer and homodimer. Nsp4 interacts with PL-PRO and nsp6. Only the homodimer shows catalytic activity. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure. Nsp9 is a dimer. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities. Nsp14 interacts (via N-terminus) with DDX1.

By similarity

Structureⁱ

3D structure databases

SMRⁱ	P0C6Y0
ModBaseⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	1083 – 1320	Peptidase C16 1PROSITE-ProRule annotationAdd BLAST	238
Domainⁱ	1321 – 1481	MacroPROSITE-ProRule annotationAdd BLAST	161
Domainⁱ	1677 – 1936	Peptidase C16 2PROSITE-ProRule annotationAdd BLAST	260
Domainⁱ	3337 – 3639	Peptidase C30PROSITE-ProRule annotationAdd BLAST	303
Domainⁱ	5068 – 5230	RdRp catalyticPROSITE-ProRule annotationAdd BLAST	163
Domainⁱ	5389 – 5472	CV ZBDPROSITE-ProRule annotationAdd BLAST	84
Domainⁱ	5644 – 5825	(+)RNA virus helicase ATP-bindingAdd BLAST	182
Domainⁱ	5826 – 6003	(+)RNA virus helicase C-terminalAdd BLAST	178

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	2228 – 2465	HD1Add BLAST	238
Regionⁱ	2846 – 3225	HD2Add BLAST	380
Regionⁱ	3648 – 3866	HD3Add BLAST	219

Domainⁱ

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Sequence similaritiesⁱ

Belongs to the coronaviruses polyprotein 1ab family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	1197 – 1225	C4-type 1PROSITE-ProRule annotationAdd BLAST	29
Zinc fingerⁱ	1793 – 1829	C4-type 2PROSITE-ProRule annotationAdd BLAST	37
Zinc fingerⁱ	4397 – 4413	By similarityAdd BLAST	17
Zinc fingerⁱ	4439 – 4452	By similarityAdd BLAST	14

Keywords - Domainⁱ

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd18808, SF1_C_Upf1, 1 hit
Gene3Dⁱ	1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.20.25.360, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.40.220.10, 1 hit 3.40.50.11580, 1 hit 3.90.70.90, 2 hits
InterProⁱ	View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR022570, B-CoV_NSP1 IPR043609, CoV_NSP15_C IPR043608, CoV_NSP15_M IPR043606, CoV_NSP15_N IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR027352, CV_ZBD IPR043502, DNA/RNA_pol_sf IPR041679, DNA2/NAM7-like_C IPR037227, EndoU-like IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR009466, NSP14_CoV IPR043174, NSP15_middle_sf IPR042515, NSP15_N_CoV IPR009461, NSP16_CoV-like IPR032592, NSP3_NAR_bCoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR027417, P-loop_NTPase IPR002705, Pept_C30/C16_B_coronavir IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus IPR009469, RNA_pol_N_coronovir IPR018995, RNA_synth_NSP10_CoV IPR029063, SAM-dependent_MTases
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13087, AAA_12, 1 hit PF16251, bCoV_NAR, 1 hit PF06471, CoV_Methyltr_1, 1 hit PF06460, CoV_Methyltr_2, 1 hit PF09401, CoV_NSP10, 1 hit PF19215, CoV_NSP15_C, 1 hit PF19216, CoV_NSP15_M, 1 hit PF19219, CoV_NSP15_N, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF06478, CoV_RPol_N, 1 hit PF11963, DUF3477, 2 hits PF01661, Macro, 1 hit PF01831, Peptidase_C16, 1 hit PF05409, Peptidase_C30, 1 hit PF00680, RdRP_1, 1 hit
SMARTⁱ	View protein in SMART SM00506, A1pp, 1 hit
SUPFAMⁱ	SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF142877, SSF142877, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF52949, SSF52949, 1 hit SSF53335, SSF53335, 2 hits SSF56672, SSF56672, 1 hit
PROSITEⁱ	View protein in PROSITE PS51653, CV_ZBD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by ribosomal frameshifting. Align Add to basket

Isoform Replicase polyprotein 1ab (identifier: P0C6Y0-1) [UniParc]FASTA Add to basket

Also known as: pp1ab

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MAKMGKYGLG FKWAPEFPWM LPNASEKLGN PERSEEDGFC PSAAQEPKVK 
        60         70         80         90        100
GKTLVNHVRV DCSRLPALEC CVQSAIIRDI FVDEDPQKVE ASTMMALQFG 
       110        120        130        140        150
SAVLVKPSKR LSVQAWAKLG VLPKTPAMGL FKRFCLCNTR ECVCDAHVAF 
       160        170        180        190        200
QLFTVQPDGV CLGNGRFIGW FVPVTAIPEY AKQWLQPWSI LLRKGGNKGS 
       210        220        230        240        250
VTSGHFRRAV TMPVYDFNVE DACEEVHLNP RGKYSCKAYA LLRGYRGVKP 
       260        270        280        290        300
ILFVDQYGCD YTGCLAKGLE DYGDLTLSEM KELSPVWRDS LDNEVVVAWH 
       310        320        330        340        350
VDRDPRAVMR LQTLATVRSI EYVGQPIEDM VDGDVVMREP AHLLAPNAIV 
       360        370        380        390        400
KRLPRLVETM LYTDSSVTEF CYKTKLCDCG FITQFGYVDC CGDTCGFRGW 
       410        420        430        440        450
VPGNMMDGFP CPGCCKSYMP WELEAQSSGV IPEGGVLFTQ STDTVNRESF 
       460        470        480        490        500
KLYGHAVVPF GGAAYWSPYP GMWLPVIWSS VKSYSYLTYT GVVGCKAIVQ 
       510        520        530        540        550
ETDAICRFLY MDYVQHKCGN LEQRAILGLD DVYHRQLLVN RGDYSLLLEN 
       560        570        580        590        600
VDLFVKRRAE FACKFATCGD GLVPLLLDGL VPRSYYLIKS GQAFTSLMVN 
       610        620        630        640        650
FSREVVDMCM DMALLFMHDV KVATKYVKKV TGKVAVRFKA LGIAVVRKIT 
       660        670        680        690        700
EWFDLAVDTA ASAAGWLCYQ LVNGLFAVAN GVITFIQEVP ELVKNFVDKF 
       710        720        730        740        750
KTFFKVLIDS MSVSILSGLT VVKTASNRVC LAGSKVYEVV QKSLPAYIMP 
       760        770        780        790        800
VGCSEATCLV GEIEPAVFED DVVDVVKAPL TYQGCCKPPS SFEKICIVDK 
       810        820        830        840        850
LYMAKCGDQF YPVVVDNDTV GVLDQCWRFP CAGKKVVFND KPKVKEVPST 
       860        870        880        890        900
RKIKIIFALD ATFDSVLSKA CSEFEVDKDV TLDELLDVVL DAVESTLSPC 
       910        920        930        940        950
KEHGVIGTKV CALLERLVDD YVYLFDEGGE EVIASRMYCS FSAPDEDCVA 
       960        970        980        990       1000
TDVVYADENQ DDDADDPVVL VADTQEEDGV AREQVDSADS EICVAHTGGQ 
      1010       1020       1030       1040       1050
EMTEPDVVGS QTPIASAEET EVGEACDREG IAEVKATVCA DALDACPDQV 
      1060       1070       1080       1090       1100
EAFDIEKVED SILSELQTEL NAPADKTYED VLAFDAIYSE TLSAFYAVPS 
      1110       1120       1130       1140       1150
DETHFKVCGF YSPAIERTNC WLRSTLIVMQ SLPLEFKDLG MQKLWLSYKA 
      1160       1170       1180       1190       1200
GYDQCFVDKL VKSAPKSIIL PQGGYVADFA YFFLSQCSFK VHANWRCLKC 
      1210       1220       1230       1240       1250
GMELKLQGLD AVFFYGDVVS HMCKCGNSMT LLSADIPYTF DFGVRDDKFC 
      1260       1270       1280       1290       1300
AFYTPRKVFR AACAVDVNDC HSMAVVDGKQ IDGKVVTKFN GDKFDFMVGH 
      1310       1320       1330       1340       1350
GMTFSMSPFE IAQLYGSCIT PNVCFVKGDV IKVLRRVGAE VIVNPANGRM 
      1360       1370       1380       1390       1400
AHGAGVAGAI AKAAGKAFIN ETADMVKAQG VCQVGGCYES TGGKLCKKVL 
      1410       1420       1430       1440       1450
NIVGPDARGH GNECYSLLER AYQHINKCDN VVTTLISAGI FSVPTDVSLT 
      1460       1470       1480       1490       1500
YLLGVVTKNV ILVSNNQDDF DVIEKCQVTS VAGTKALSFQ LAKNLCRDVK 
      1510       1520       1530       1540       1550
FVTNACSSLF SESSFVSSYD VLQEVEALRH DIQLDDDARV FVQANMDCLP 
      1560       1570       1580       1590       1600
TDWRLVNKFD SVDGVRTIKY FECPGEVFVS SQGKKFGYVQ NGSFKEASVS 
      1610       1620       1630       1640       1650
QIRALLANKV DVLCTVDGVN FRSCCVAEGE VFGKTLGSVF CDGINVTKVR 
      1660       1670       1680       1690       1700
CSAIHKGKVF FQYSGLSAAD LAAVKDAFGF DEPQLLQYYS MLGMCKWPVV 
      1710       1720       1730       1740       1750
VCGNYFAFKQ SNNNCYINVA CLMLQHLSLK FPKWQWRRPG NEFRSGKPLR 
      1760       1770       1780       1790       1800
FVSLVLAKGS FKFNEPSDST DFIRVELREA DLSGATCDLE FICKCGVKQE 
      1810       1820       1830       1840       1850
QRKGVDAVMH FGTLDKSGLV KGYNIACTCG DKLVHCTQFN VPFLICSNTP 
      1860       1870       1880       1890       1900
EGKKLPDDVV AANIFTGGSV GHYTHVKCKP KYQLYDACNV SKVSEAKGNF 
      1910       1920       1930       1940       1950
TDCLYLKNLK QTFSSVLTTY YLDDVKCVAY KPDLSQYYCE SGKYYTKPII 
      1960       1970       1980       1990       2000
KAQFRTFEKV EGVYTNFKLV GHDIAEKLNA KLGFDCNSPF MEYKITEWPT 
      2010       2020       2030       2040       2050
ATGDVVLASD DLYVSRYSGG CVTFGKPVIW RGHEEASLKS LTYFNRPSVV 
      2060       2070       2080       2090       2100
CENKFNVLPV DVSEPTDRRP VPSAVLVTGA ASGADASAIS TEPGTAKEQK 
      2110       2120       2130       2140       2150
ACASDSVEDQ IVMEAQKKSS VTTVAVKEVK LNGVKKPVKW NCSVVVNDPT 
      2160       2170       2180       2190       2200
SETKVVKSLS IVDVYDMFLT GCRYVVWTAN ELSRLINSPT VREYVKWGMS 
      2210       2220       2230       2240       2250
KLIIPANLLL LRDEKQEFVA PKVVKAKAIA CYGAVKWFLL YCFSWIKFNT 
      2260       2270       2280       2290       2300
DNKVIYTTEV ASKLTFKLCC LAFKNALQTF NWSVVSRGFF LVATVFLLWF 
      2310       2320       2330       2340       2350
NFLYANVILS DFYLPNIGPL PMFVGQIVAW VKTTFGVLTI CDFYQVTDLG 
      2360       2370       2380       2390       2400
YRSSFCNGSM VCELCFSGFD MLDNYESINV VQHVVDRRVS FDYISLFKLV 
      2410       2420       2430       2440       2450
VELVIGYSLY TVCFYPLFVL VGMQLLTTWL PEFFMLGTMH WSARLFVFVA 
      2460       2470       2480       2490       2500
NMLPAFTLLR FYIVVTAMYK VYCLCRHVMY GCSKPGCLFC YKRNRSVRVK 
      2510       2520       2530       2540       2550
CSTVVGGSLR YYDVMANGGT GFCTKHQWNC LNCNSWKPGN TFITHEAAAD 
      2560       2570       2580       2590       2600
LSKELKRPVN PTDSAYYSVI EVKQVGCSMR LFYERDGQRV YDDVSASLFV 
      2610       2620       2630       2640       2650
DMNGLLHSKV KGVPETHVVV VENEADKAGF LNAAVFYAQS LYRPMLMVEK 
      2660       2670       2680       2690       2700
KLITTANTGL SVSRTMFDLY VYSLLRHLDV DRKSLTSFVN AAHNSLKEGV 
      2710       2720       2730       2740       2750
QLEQVMDTFV GCARRKCAID SDVETKSITK SVMAAVNAGV EVTDESCNNL 
      2760       2770       2780       2790       2800
VPTYVKSDTI VAADLGVLIQ NNAKHVQSNV AKAANVACIW SVDAFNQLSA 
      2810       2820       2830       2840       2850
DLQHRLRKAC VKTGLKIKLT YNKQEANVPI LTTPFSLKGG AVFSRVLQWL 
      2860       2870       2880       2890       2900
FVANLICFIV LWALMPTYAV HKSDMQLPLY ASFKVIDNGV LRDVSVTDAC 
      2910       2920       2930       2940       2950
FANKFNQFDQ WYESTFGLVY YRNSKACPVV VAVIDQDIGH TLFNVPTKVL 
      2960       2970       2980       2990       3000
RYGFHVLHFI THAFATDRVQ CYTPHMQIPY DNFYASGCVL SSLCTMLAHA 
      3010       3020       3030       3040       3050
DGTPHPYCYT EGVMHNASLY SSLVPHVRYN LASSNGYIRF PEVVSEGIVR 
      3060       3070       3080       3090       3100
VVRTRSMTYC RVGLCEEAEE GICFNFNSSW VLNNPYYRAM PGTFCGRNAF 
      3110       3120       3130       3140       3150
DLIHQVLGGL VQPIDFFALT ASSVAGAILA IIVVLAFYYL IKLKRAFGDY 
      3160       3170       3180       3190       3200
TSVVVINVIV WCINFLMLFV FQVYPTLSCL YACFYFYTTL YFPSEISVVM 
      3210       3220       3230       3240       3250
HLQWLVMYGA IMPLWFCITY VAVVVSNHAL WLFSYCRKIG TDVRSDGTFE 
      3260       3270       3280       3290       3300
EMALTTFMIT KESYCKLKNS VSDVAFNRYL SLYNKYRYFS GKMDTATYRE 
      3310       3320       3330       3340       3350
AACSQLAKAM ETFNHNNGND VLYQPPTASV TTSFLQSGIV KMVSPTSKVE 
      3360       3370       3380       3390       3400
PCVVSVTYGN MTLNGLWLDD KVYCPRHVIC SSADMTDPDY PNLLCRVTSS 
      3410       3420       3430       3440       3450
DFCVMSDRMS LTVMSYQMQG SLLVLTVTLQ NPNTPKYSFG VVKPGETFTV 
      3460       3470       3480       3490       3500
LAAYNGRPQG AFHVVMRSSH TIKGSFLCGS CGSVGYVLTG DSVRFVYMHQ 
      3510       3520       3530       3540       3550
LELSTGCHTG TDFSGNFYGP YRDAQVVQLP VQDYTQTVNV VAWLYAAILN 
      3560       3570       3580       3590       3600
RCNWFVQSDS CSLEEFNVWA MTNGFSSIKA DLVLDALASM TGVTVEQVLA 
      3610       3620       3630       3640       3650
AIKRLHSGFQ GKQILGSCVL EDELTPSDVY QQLAGVKLQS KRTRVIKGTC 
      3660       3670       3680       3690       3700
CWILASTFLF CSIISAFVKW TMFMYVTTHM LGVTLCALCF VIFAMLLIKH 
      3710       3720       3730       3740       3750
KHLYLTMYIM PVLCTLFYTN YLVVGYKQSF RGLAYAWLSY FVPAVDYTYM 
      3760       3770       3780       3790       3800
DEVLYGVVLL VAMVFVTMRS INHDVFSTMF LVGRLVSLVS MWYFGANLEE 
      3810       3820       3830       3840       3850
EVLLFLTSLF GTYTWTTMLS LATAKVIAKW LAVNVLYFTD IPQIKLVLLS 
      3860       3870       3880       3890       3900
YLCIGYVCCC YWGVLSLLNS IFRMPLGVYN YKISVQELRY MNANGLRPPR 
      3910       3920       3930       3940       3950
NSFEALMLNF KLLGIGGVPV IEVSQIQSRL TDVKCANVVL LNCLQHLHIA 
      3960       3970       3980       3990       4000
SNSKLWQYCS TLHNEILATS DLSVAFDKLA QLLVVLFANP AAVDSKCLAS 
      4010       4020       4030       4040       4050
IEEVSDDYVR DNTVLQALQS EFVNMASFVE YELAKKNLDE AKASGSANQQ 
      4060       4070       4080       4090       4100
QIKQLEKACN IAKSAYERDR AVARKLERMA DLALTNMYKE ARINDKKSKV 
      4110       4120       4130       4140       4150
VSALQTMLFS MVRKLDNQAL NSILDNAVKG CVPLNAIPPL TSNTLTIIVP 
      4160       4170       4180       4190       4200
DKQVFDQVVD NVYVTYAPNV WHIQSIQDAD GAVKQLNEID VNSTWPLVIS 
      4210       4220       4230       4240       4250
ANRHNEVSTV VLQNNELMPQ KLRTQVVNSG SDMNCNIPTQ CYYNTTGTGK 
      4260       4270       4280       4290       4300
IVYAILSDCD GLKYTKIVKE DGNCVVLELD PPCKFSVQDV KGLKIKYLYF 
      4310       4320       4330       4340       4350
VKGCNTLARG WVVGTLSSTV RLQAGTATEY ASNSAILSLC AFSVDPKKTY 
      4360       4370       4380       4390       4400
LDYIQQGGVP VTNCVKMLCD HAGTGMAITI KPEATTNQDS YGGASVCIYC 
      4410       4420       4430       4440       4450
RSRVEHPDVD GLCKLRGKFV QVPLGIKDPV SYVLTHDVCQ VCGFWRDGSC 
      4460       4470       4480       4490       4500
SCVGTGSQFQ SKDTNFLNRV RGTSVNARLV PCASGLDTDV QLRAFDICNA 
      4510       4520       4530       4540       4550
NRAGIGLYYK VNCFRFQRVD EEGNKLDKFF VVKRTNLEVY NKEKECYELT 
      4560       4570       4580       4590       4600
KDCGVVAEHE FFTFDVEGSR VPHIVRKDLS KFTMLDLCYA LRHFDRNDCS 
      4610       4620       4630       4640       4650
TLKEILLTYA ECDESYFQKK DWYDFVENPD IINVYKKLGP IFNRALLNTA 
      4660       4670       4680       4690       4700
NFADTLVEAG LVGVLTLDNQ DLYGQWYDFG DFVKTVPCCG VAVADSYYSY 
      4710       4720       4730       4740       4750
MMPMLTMCHA LDSELFVNGT YREFDLVQYD FTDFKLELFN KYFKHWSMTY 
      4760       4770       4780       4790       4800
HPNTSECEDD RCIIHCANFN ILFSMVLPKT CFGPLVRQIF VDGVPFVVSI 
      4810       4820       4830       4840       4850
GYHYKELGVV MNMDVDTHRY RLSLKDLLLY AADPALHVAS ASALLDLRTC 
      4860       4870       4880       4890       4900
CFSVAAITSG VKFQTVKPGN FNQDFYEFIL SKGLLKEGSS VDLKHFFFTQ 
      4910       4920       4930       4940       4950
DGNAAITDYN YYKYNLPTMV DIKQLLFVVE VVNKYFEIYE GGCIPATQVI 
      4960       4970       4980       4990       5000
VNNYDKSAGY PFNKFGKARL YYEALSFEEQ DEIYAYTKRN VLPTLTQMNL 
      5010       5020       5030       5040       5050
KYAISAKNRA RTVAGVSILS TMTGRMFHQK CLKSIAATRG VPVVIGTTKF 
      5060       5070       5080       5090       5100
YGGWDDMLRR LIKDVDSPVL MGWDYPKCDR AMPNILRIVS SLVLARKHDS 
      5110       5120       5130       5140       5150
CCSHTDRFYR LANECAQVLG EIVMCGGCYY VKPGGTSSGD ATTAFANSVF 
      5160       5170       5180       5190       5200
NICQAVSANV CSLMACNGHK IEDLSIRELQ KRLYSNVYRA DHVDPAFVSE 
      5210       5220       5230       5240       5250
YYEFLNKHFS MIILSDDGVV CYNSEFASKG YIANISDFQQ VLYYQNNVFM 
      5260       5270       5280       5290       5300
SEAKCWVETD IEKGPHEFCS QHTMLVKMDG DEVYLPYPDP SRILGAGCFV 
      5310       5320       5330       5340       5350
DDLLKTDSVL LIERFVSLAI DAYPLVYHEN PEYQNVFRVY LEYIKKLYND 
      5360       5370       5380       5390       5400
LGNQILDSIS VILSTCDGQK FTDETFYKNM YLRSAVMQSV GACVVCSSQT 
      5410       5420       5430       5440       5450
SLRCGSCIRK PLLCCKCAYD HVMSTDHKYV LSVSPYVCNS PGCDVNDVTK 
      5460       5470       5480       5490       5500
LYLGGMSYYC EAHKPQYSFK LVMNGMVFGL YKQSCTGSPY IEDFNKIASC 
      5510       5520       5530       5540       5550
KWTEVDDYVL ANECTERLKL FAAETQKATE EAFKQCYASA TIREIVSDRE 
      5560       5570       5580       5590       5600
LILSWEIGKV RPPLNKNYVF TGYHFTNNGK TVLGEYVFDK SELTNGVYYR 
      5610       5620       5630       5640       5650
ATTTYKLSVG DVFILTSHAV SSLSAPTLVP QENYTSVRFA SAYSVPETFQ 
      5660       5670       5680       5690       5700
NNVPNYQHIG IKRYCTVQGP PGTGKSHLAI GHAVYYCTAR VVYTAASHAA 
      5710       5720       5730       5740       5750
VDALCEKAHK FLNINDCARI VPAKLRVDCY DKFNVNDTTR KYVFTTINAL 
      5760       5770       5780       5790       5800
PELVTDIIVV DEVSMLTNYE LSVINSRVRA KHYVYIGDPA QLPAPRVLLN 
      5810       5820       5830       5840       5850
KGTLEPRYFN SVTKLMCCLG PDIFLGTCYR CPKEIVDTVS ALVYNNKLKA 
      5860       5870       5880       5890       5900
KNDNSAMCFK VYYKGQTTHE SSSAVNMQQI HLISKLLKAN PSWSNAVFIS 
      5910       5920       5930       5940       5950
PYNSQNYVAK RVLGLQTQTA DSAQGSAYDF VIYSQTAQTA HSVNVNRFNV 
      5960       5970       5980       5990       6000
AITRAKKGIL CVMSSMQLIG VFNFTTLTLD KINNPRLQCT TNLFKDCSKS 
      6010       6020       6030       6040       6050
YVGIPPCAFL LAVDDKYKVS GNLAVCLNVA DSAVTYSRLI SLMGFKLDLT 
      6060       6070       6080       6090       6100
LDGYCKLFIT RDEAIKRVRA WVGFDAEGAH ATRDSIGTNF PLQLGFSTGI 
      6110       6120       6130       6140       6150
DFVVEATGMF AERDGYVFKK AAARAPPGEQ FKHLVPLMSR GQKWDVVRIR 
      6160       6170       6180       6190       6200
IVQMLSDHLV DLADSVVLVT WAASFELTCL RYFAKVGKEV VCSVCNKRAT 
      6210       6220       6230       6240       6250
CFNSRTGYYG CWRHSYSCDY LYNPLIVDIQ QWGYTGSLTS NHDPICSVHK 
      6260       6270       6280       6290       6300
GAHVASSDAI MTRCLAVHDC FCKSVNWNLE YPIISNEVSV NTSCRLLQRV 
      6310       6320       6330       6340       6350
MFRAAMLCNR YDVCYDIGNP KGLACVKGYD FKFYDASPVV KSVKQFVYKY 
      6360       6370       6380       6390       6400
EAHKDQFLDG LCMFWNCNVD KYPANAVVCR FDTRVLSKLN LPGCNGGSLY 
      6410       6420       6430       6440       6450
VNKHAFHTNP FTRAAFENLK PMPFFYYSDT PCVYMEGMES KQVDYVPLRS 
      6460       6470       6480       6490       6500
ATCITRCNLG GAVCLKHAEE YREYLESYNT ATTAGFTFWV YKTFDFYNLW 
      6510       6520       6530       6540       6550
NTFTRLQSLE NVVYNLVNAG HFDGRAGELP CAVIGEKVIA KIQNEDVVVF 
      6560       6570       6580       6590       6600
KNNTPFPTNV AVELFAERSI RPHPELKLFR SSNIHVCWNH VLWDYAKDSV 
      6610       6620       6630       6640       6650
FCSSTYKVCK YTDLQCIESL NVLFDGRDNG ALEAFKKCRN GVYINTTKIK 
      6660       6670       6680       6690       6700
SLSMIKGPQR ADLNGVVVEK VGDSDVEFWF AMRRDGDDVI FSRTGSLEPS 
      6710       6720       6730       6740       6750
HYRSPQGNPG GNRVGDLSGN EALARGTIFT QSRFLSSFSP RSEMEKDFMD 
      6760       6770       6780       6790       6800
LDEDVFIAKY SLQDYAFEHV VYGSFNQKII GGLHLLIGLA RRPKKSNLVI 
      6810       6820       6830       6840       6850
QEFVPYDSSI HSYFITDENS GSSESVCTVI DLLLDDFVDI VKSLNLKCVS 
      6860       6870       6880       6890       6900
KVVNVNVDFK DFQFMLWCNE EKVMTFYPRL QAAADWKPGY VMPVLYKYLE 
      6910       6920       6930       6940       6950
SPMERVNLWN YGKPITLPTG CMMNVAKYTQ LCQYLSTTTL AVPANMRVLH 
      6960       6970       6980       6990       7000
LGAGSDKGVA PGSAVLRQWL PSGSILVDND MNPFVSDSVA SYYGNCITLP 
      7010       7020       7030       7040       7050
FDCQWDLIIS DMYDPLTKNI GEYNVSKDGF FTYLCHLIRD KLALGGSVAI 
      7060       7070       7080       7090       7100
KITEFSWNAE LYSLMGKFAF WTIFCTNVNA SSSEGFLIGI NWLNRTRNEI 
      7110       7120       7130       7140       7150
DGKTMHANYL FWRNSTMWNG GAYSLFDMTK FPLKAAGTAV VSLKPDQIND 
      7160       7170       7180
LVLSLIEKGK LLVRDTRKEV FVGDSLVNVK

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,180

Mass (Da):803,440

Last modified:June 10, 2008 - v1

Checksum:ⁱ313A78E1CC87B347

GO

Isoform Replicase polyprotein 1a (identifier: P0C6V1-1) [UniParc]FASTA Add to basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6V1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Length:4,474

Mass (Da):497,595

GO

Sequence cautionⁱ

The sequence AAA46457 differs from that shown. Reason: Frameshift.Curated

The sequence AAA46457 differs from that shown. Reason: Erroneous gene model prediction.Curated

The sequence AAA46458 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M55148 Genomic RNA Translation: AAA46457.1 Sequence problems. M55148 Genomic RNA Translation: AAA46458.2 Sequence problems. M18040 Genomic RNA Translation: AAA46466.1 S51684 Genomic RNA Translation: AAB19566.1
PIRⁱ	A36815, RRIHM2 B36815, VFIHJH
NCBI Reference Sequences More...RefSeqⁱ	YP_209229.2, AC_000192.1 [P0C6Y0-1]

Keywords - Coding sequence diversityⁱ

Ribosomal frameshifting

Protein	Similar proteins		Species	Score	Length	Source
P0C6Y0	Replicase polyprotein 1ab	)	CVMA5		7176	UniRef90_P0C6X9
Replicase polyprotein 1ab (Fragment)		CVRSD		307
3C-like proteinase		Murine coronavirus		7181
1 protein (Fragment)		Murine hepatitis virus		46
3C-like proteinase		Murine hepatitis virus strain S/3239-17		7179
+78

Protein	Similar proteins		Species	Score	Length	Source
P0C6Y0	Replicase polyprotein 1ab		CVHOC		7095	UniRef50_P0C6X6
Replicase polyprotein 1ab		CVBM		7094
Replicase polyprotein 1ab		CVBEN		7094
Replicase polyprotein 1ab		CVBLU		7094
Replicase polyprotein 1ab		CVBQ		7059
+1027

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M55148 Genomic RNA Translation: AAA46457.1 Sequence problems. M55148 Genomic RNA Translation: AAA46458.2 Sequence problems. M18040 Genomic RNA Translation: AAA46466.1 S51684 Genomic RNA Translation: AAB19566.1
PIRⁱ	A36815, RRIHM2 B36815, VFIHJH
RefSeqⁱ	YP_209229.2, AC_000192.1 [P0C6Y0-1]

3D structure databases

SMRⁱ	P0C6Y0
ModBaseⁱ	Search...

Proteomic databases

PRIDEⁱ	P0C6Y0

PRIDEⁱ

P0C6Y0

Family and domain databases

CDDⁱ	cd18808, SF1_C_Upf1, 1 hit
Gene3Dⁱ	1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.20.25.360, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.40.220.10, 1 hit 3.40.50.11580, 1 hit 3.90.70.90, 2 hits
InterProⁱ	View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR022570, B-CoV_NSP1 IPR043609, CoV_NSP15_C IPR043608, CoV_NSP15_M IPR043606, CoV_NSP15_N IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR027352, CV_ZBD IPR043502, DNA/RNA_pol_sf IPR041679, DNA2/NAM7-like_C IPR037227, EndoU-like IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR009466, NSP14_CoV IPR043174, NSP15_middle_sf IPR042515, NSP15_N_CoV IPR009461, NSP16_CoV-like IPR032592, NSP3_NAR_bCoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR027417, P-loop_NTPase IPR002705, Pept_C30/C16_B_coronavir IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus IPR009469, RNA_pol_N_coronovir IPR018995, RNA_synth_NSP10_CoV IPR029063, SAM-dependent_MTases
Pfamⁱ	View protein in Pfam PF13087, AAA_12, 1 hit PF16251, bCoV_NAR, 1 hit PF06471, CoV_Methyltr_1, 1 hit PF06460, CoV_Methyltr_2, 1 hit PF09401, CoV_NSP10, 1 hit PF19215, CoV_NSP15_C, 1 hit PF19216, CoV_NSP15_M, 1 hit PF19219, CoV_NSP15_N, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF06478, CoV_RPol_N, 1 hit PF11963, DUF3477, 2 hits PF01661, Macro, 1 hit PF01831, Peptidase_C16, 1 hit PF05409, Peptidase_C30, 1 hit PF00680, RdRP_1, 1 hit
SMARTⁱ	View protein in SMART SM00506, A1pp, 1 hit
SUPFAMⁱ	SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF142877, SSF142877, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF52949, SSF52949, 1 hit SSF53335, SSF53335, 2 hits SSF56672, SSF56672, 1 hit
PROSITEⁱ	View protein in PROSITE PS51653, CV_ZBD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	R1AB_CVMJH
Accessionⁱ	P0C6Y0Primary (citable) accession number: P0C6Y0 Secondary accession number(s): P19751 , P29982, Q66194, Q90045
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
	Last sequence update:	June 10, 2008
	Last modified:	December 2, 2020
	This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

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UniProtKB - P0C6Y0 (R1AB_CVMJH)

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Replicase polyprotein 1ab

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Functionⁱ

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Names & Taxonomyⁱ

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GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

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Sequence cautionⁱ

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