UniProtKB - P0C6V3 (R1A_IBVB)
Protein
Replicase polyprotein 1a
Gene
1a
Organism
Avian infectious bronchitis virus (strain Beaudette) (IBV)
Status
Functioni
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).By similarity
Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.PROSITE-ProRule annotation
The peptide p16 might be involved in the EGF signaling pathway.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1274 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1437 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 2820 | For 3CL-PRO activity | 1 | |
Active sitei | 2922 | For 3CL-PRO activity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1353 – 1390 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 3858 – 3878 | By similarityAdd BLAST | 21 | |
Zinc fingeri | 3904 – 3917 | By similarityAdd BLAST | 14 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: InterPro
- identical protein binding Source: IntAct
- omega peptidase activity Source: InterPro
- RNA binding Source: UniProtKB-KW
- transferase activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- induction by virus of host autophagy Source: UniProtKB-KW
- viral genome replication Source: InterPro
- viral protein processing Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, RNA-binding, Thiol protease |
Biological process | Activation of host autophagy by virus, Host-virus interaction |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.22.B14, 8728 |
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1aShort name: pp1a Alternative name(s): ORF1a polyprotein Cleaved into the following 10 chains: Alternative name(s): p87 Alternative name(s): Papain-like proteinase Short name: PL-PRO p195 Alternative name(s): Peptide HD2 p41 Alternative name(s): M-PRO nsp5 p33 Alternative name(s): p34 Alternative name(s): p9 Alternative name(s): p24 Alternative name(s): p10 Alternative name(s): Growth factor-like peptide Short name: GFL p16 |
Gene namesi | ORF Names:1a |
Organismi | Avian infectious bronchitis virus (strain Beaudette) (IBV) |
Taxonomic identifieri | 11122 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Gammacoronavirus › Igacovirus › |
Virus hosti | Gallus gallus (Chicken) [TaxID: 9031] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1751 – 1771 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1844 – 1864 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1887 – 1907 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2281 – 2301 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2560 – 2580 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2589 – 2609 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2612 – 2632 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2644 – 2664 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3101 – 3121 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3122 – 3142 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3152 – 3172 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3189 – 3209 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3220 – 3240 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3258 – 3278 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3297 – 3317 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- host cell membrane Source: UniProtKB-SubCell
- host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 673 | G → A: No processing between p87 and p195. 1 Publication | 1 | |
Mutagenesisi | 676 | T → S: No effect. 1 Publication | 1 | |
Mutagenesisi | 1274 | C → S: Complete loss of PL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1437 | H → K: Complete loss of PL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 2264 | A → N: Almost no processing between p195 and peptide HD2. 1 Publication | 1 | |
Mutagenesisi | 2265 – 2266 | Missing : No processing between p195 and peptide HD2. 1 Publication | 2 | |
Mutagenesisi | 2265 | G → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 2265 | G → N: Almost no processing between p195 and peptide HD2. 1 Publication | 1 | |
Mutagenesisi | 2266 | G → N: No effect. 1 Publication | 1 | |
Mutagenesisi | 2820 | H → K or G: Complete loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 2841 | E → Q: No effect. 1 Publication | 1 | |
Mutagenesisi | 2843 | E → D, N or Q: No effect. 1 Publication | 1 | |
Mutagenesisi | 2922 | C → A: Complete loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 2922 | C → S: Partial loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3086 | Q → E: No processing between 3CL-PRO and p34. 1 Publication | 1 | |
Mutagenesisi | 3365 | Q → E: No effect. 1 Publication | 1 | |
Mutagenesisi | 3379 | Q → E: No processing between p34 and p9. 1 Publication | 1 | |
Mutagenesisi | 3462 | Q → E: No processing between p9 and p24. 1 Publication | 1 | |
Mutagenesisi | 3672 | Q → E: No processing between p24 and p10. 2 Publications | 1 | |
Mutagenesisi | 3783 | Q → E: No processing between p10 and p16. 2 Publications | 1 | |
Mutagenesisi | 3928 | Q → E: No processing between p16 and p100. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000338314 | 1 – 3951 | Replicase polyprotein 1aAdd BLAST | 3951 | |
ChainiPRO_0000338315 | 1 – 673 | Non-structural protein 2Add BLAST | 673 | |
ChainiPRO_0000338316 | 674 – 2265 | Non-structural protein 3Add BLAST | 1592 | |
ChainiPRO_0000338317 | 2266 – 2779 | Non-structural protein 4Add BLAST | 514 | |
ChainiPRO_0000338318 | 2780 – 3086 | 3C-like proteinaseAdd BLAST | 307 | |
ChainiPRO_0000338319 | 3087 – 3379 | Non-structural protein 6Add BLAST | 293 | |
ChainiPRO_0000338320 | 3380 – 3462 | Non-structural protein 7Add BLAST | 83 | |
ChainiPRO_0000338321 | 3463 – 3672 | Non-structural protein 8Add BLAST | 210 | |
ChainiPRO_0000338322 | 3673 – 3783 | Non-structural protein 9Add BLAST | 111 | |
ChainiPRO_0000338323 | 3784 – 3928 | Non-structural protein 10Add BLAST | 145 | |
ChainiPRO_0000338324 | 3929 – 3951 | Non-structural protein 11Sequence analysisAdd BLAST | 23 |
Post-translational modificationi
Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity
P41 is N-glycosylated.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 673 – 674 | Cleavage; by PL-PRO | 2 | |
Sitei | 2265 – 2266 | Cleavage; by PL-PRO | 2 | |
Sitei | 2779 – 2780 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3086 – 3087 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3379 – 3380 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3462 – 3463 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3672 – 3673 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3783 – 3784 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3928 – 3929 | Cleavage; by 3CL-PRO | 2 |
Interactioni
Subunit structurei
3CL-PRO exists as monomer and homodimer. The three peptides nsp2, nsp3 and nsp4 may form a complex. Nsp9 is a dimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp10 forms a dodecamer (By similarity).
By similarityBinary interactionsi
Non-structural protein 9 (PRO_0000338322)
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-25618172,EBI-25618172 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1003 – 1179 | MacroPROSITE-ProRule annotationAdd BLAST | 177 | |
Domaini | 1236 – 1497 | Peptidase C16PROSITE-ProRule annotationAdd BLAST | 262 | |
Domaini | 2780 – 3086 | Peptidase C30PROSITE-ProRule annotationAdd BLAST | 307 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1721 – 1907 | HD1Add BLAST | 187 | |
Regioni | 2281 – 2664 | HD2Add BLAST | 384 | |
Regioni | 3101 – 3317 | HD3Add BLAST | 217 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 226 – 229 | Poly-Val | 4 | |
Compositional biasi | 2570 – 2573 | Poly-Val | 4 |
Domaini
The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.
Sequence similaritiesi
Belongs to the coronaviruses polyprotein 1ab family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1353 – 1390 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 3858 – 3878 | By similarityAdd BLAST | 21 | |
Zinc fingeri | 3904 – 3917 | By similarityAdd BLAST | 14 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.40.220.10, 1 hit 3.90.70.90, 1 hit |
InterProi | View protein in InterPro IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR040795, Nsp1a_N IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043503, PLpro_palm_finger_dom_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF09401, CoV_NSP10, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF01661, Macro, 1 hit PF17896, Nsp2a_N, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform Replicase polyprotein 1a (identifier: P0C6V3-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASSLKQGVS PKPRDVILVS KDIPEQLCDA LFFYTSHNPK DYADAFAVRQ
60 70 80 90 100
KFDRSLQTGK QFKFETVCGL FLLKGVDKIT PGVPAKVLKA TSKLADLEDI
110 120 130 140 150
FGVSPLARKY RELLKTACQW SLTVEALDVR AQTLDEIFDP TEILWLQVAA
160 170 180 190 200
KIHVSSMAMR RLVGEVTAKV MDALGSNLSA LFQIVKQQIA RIFQKALAIF
210 220 230 240 250
ENVNELPQRI AALKMAFAKC ARSITVVVVE RTLVVKEFAG TCLASINGAV
260 270 280 290 300
AKFFEELPNG FMGSKIFTTL AFFKEAAVRV VENIPNAPRG TKGFEVVGNA
310 320 330 340 350
KGTQVVVRGM RNDLTLLDQK ADIPVEPEGW SAILDGHLCY VFRSGDRFYA
360 370 380 390 400
APLSGNFALS DVHCCERVVC LSDGVTPEIN DGLILAAIYS SFSVSELVTA
410 420 430 440 450
LKKGEPFKFL GHKFVYAKDA AVSFTLAKAA TIADVLRLFQ SARVIAEDVW
460 470 480 490 500
SSFTEKSFEF WKLAYGKVRN LEEFVKTYVC KAQMSIVILA AVLGEDIWHL
510 520 530 540 550
VSQVIYKLGV LFTKVVDFCD KHWKGFCVQL KRAKLIVTET FCVLKGVAQH
560 570 580 590 600
CFQLLLDAIH SLYKSFKKCA LGRIHGDLLF WKGGVHKIVQ DGDEIWFDAI
610 620 630 640 650
DSVDVEDLGV VQEKSIDFEV CDDVTLPENQ PGHMVQIEDD GKNYMFFRFK
660 670 680 690 700
KDENIYYTPM SQLGAINVVC KAGGKTVTFG ETTVQEIPPP DVVPIKVSIE
710 720 730 740 750
CCGEPWNTIF KKAYKEPIEV DTDLTVEQLL SVIYEKMCDD LKLFPEAPEP
760 770 780 790 800
PPFENVALVD KNGKDLDCIK SCHLIYRDYE SDDDIEEEDA EECDTDSGEA
810 820 830 840 850
EECDTNSECE EEDEDTKVLA LIQDPASIKY PLPLDEDYSV YNGCIVHKDA
860 870 880 890 900
LDVVNLPSGE ETFVVNNCFE GAVKPLPQKV VDVLGDWGEA VDAQEQLCQQ
910 920 930 940 950
EPLQHTFEEP VENSTGSSKT MTEQVVVEDQ ELPVVEQDQD VVVYTPTDLE
960 970 980 990 1000
VAKETAEEVD EFILIFAVPK EEVVSQKDGA QIKQEPIQVV KPQREKKAKK
1010 1020 1030 1040 1050
FKVKPATCEK PKFLEYKTCV GDLTVVIAKA LDEFKEFCIV NAANEHMTHG
1060 1070 1080 1090 1100
SGVAKAIADF CGLDFVEYCE DYVKKHGPQQ RLVTPSFVKG IQCVNNVVGP
1110 1120 1130 1140 1150
RHGDNNLHEK LVAAYKNVLV DGVVNYVVPV LSLGIFGVDF KMSIDAMREA
1160 1170 1180 1190 1200
FEGCTIRVLL FSLSQEHIDY FDVTCKQKTI YLTEDGVKYR SIVLKPGDSL
1210 1220 1230 1240 1250
GQFGQVYAKN KIVFTADDVE DKEILYVPTT DKSILEYYGL DAQKYVIYLQ
1260 1270 1280 1290 1300
TLAQKWNVQY RDNFLILEWR DGNCWISSAI VLLQAAKIRF KGFLTEAWAK
1310 1320 1330 1340 1350
LLGGDPTDFV AWCYASCTAK VGDFSDANWL LANLAEHFDA DYTNAFLKKR
1360 1370 1380 1390 1400
VSCNCGIKSY ELRGLEACIQ PVRATNLLHF KTQYSNCPTC GANNTDEVIE
1410 1420 1430 1440 1450
ASLPYLLLFA TDGPATVDCD EDAVGTVVFV GSTNSGHCYT QAAGQAFDNL
1460 1470 1480 1490 1500
AKDRKFGKKS PYITAMYTRF AFKNETSLPV AKQSKGKSKS VKEDVSNLAT
1510 1520 1530 1540 1550
SSKASFDNLT DFEQWYDSNI YESLKVQESP DNFDKYVSFT TKEDSKLPLT
1560 1570 1580 1590 1600
LKVRGIKSVV DFRSKDGFIY KLTPDTDENS KAPVYYPVLD AISLKAIWVE
1610 1620 1630 1640 1650
GNANFVVGHP NYYSKSLHIP TFWENAENFV KMGDKIGGVT MGLWRAEHLN
1660 1670 1680 1690 1700
KPNLERIFNI AKKAIVGSSV VTTQCGKLIG KAATFIADKV GGGVVRNITD
1710 1720 1730 1740 1750
SIKGLCGITR GHFERKMSPQ FLKTLMFFLF YFLKASVKSV VASYKTVLCK
1760 1770 1780 1790 1800
VVLATLLIVW FVYTSNPVMF TGIRVLDFLF EGSLCGPYKD YGKDSFDVLR
1810 1820 1830 1840 1850
YCADDFICRV CLHDKDSLHL YKHAYSVEQV YKDAASGFIF NWNWLYLVFL
1860 1870 1880 1890 1900
ILFVKPVAGF VIICYCVKYL VLNSTVLQTG VCFLDWFVQT VFSHFNFMGA
1910 1920 1930 1940 1950
GFYFWLFYKI YIQVHHILYC KDVTCEVCKR VARSNRQEVS VVVGGRKQIV
1960 1970 1980 1990 2000
HVYTNSGYNF CKRHNWYCRN CDDYGHQNTF MSPEVAGELS EKLKRHVKPT
2010 2020 2030 2040 2050
AYAYHVVDEA CLVDDFVNLK YKAATPGKDS ASSAVKCFSV TDFLKKAVFL
2060 2070 2080 2090 2100
KEALKCEQIS NDGFIVCNTQ SAHALEEAKN AAIYYAQYLC KPILILDQAL
2110 2120 2130 2140 2150
YEQLVVEPVS KSVIDKVCSI LSSIISVDTA ALNYKAGTLR DALLSITKDE
2160 2170 2180 2190 2200
EAVDMAIFCH NHDVDYTGDG FTNVIPSYGI DTGKLTPRDR GFLINADASI
2210 2220 2230 2240 2250
ANLRVKNAPP VVWKFSELIK LSDSCLKYLI SATVKSGVRF FITKSGAKQV
2260 2270 2280 2290 2300
IACHTQKLLV EKKAGGIVSG TFKCFKSYFK WLLIFYILFT ACCSGYYYME
2310 2320 2330 2340 2350
VSKSFVHPMY DVNSTLHVEG FKVIDKGVLR EIVPEDTCFS NKFVNFDAFW
2360 2370 2380 2390 2400
GRPYDNSRNC PIVTAVIDGD GTVATGVPGF VSWVMDGVMF IHMTQTERKP
2410 2420 2430 2440 2450
WYIPTWFNRE IVGYTQDSII TEGSFYTSIA LFSARCLYLT ASNTPQLYCF
2460 2470 2480 2490 2500
NGDNDAPGAL PFGSIIPHRV YFQPNGVRLI VPQQILHTPY VVKFVSDSYC
2510 2520 2530 2540 2550
RGSVCEYTRP GYCVSLNPQW VLFNDEYTSK PGVFCGSTVR ELMFSMVSTF
2560 2570 2580 2590 2600
FTGVNPNIYM QLATMFLILV VVVLIFAMVI KFQGVFKAYA TTVFITMLVW
2610 2620 2630 2640 2650
VINAFILCVH SYNSVLAVIL LVLYCYASLV TSRNTVIIMH CWLVFTFGLI
2660 2670 2680 2690 2700
VPTWLACCYL GFIIYMYTPL FLWCYGTTKN TRKLYDGNEF VGNYDLAAKS
2710 2720 2730 2740 2750
TFVIRGSEFV KLTNEIGDKF EAYLSAYARL KYYSGTGSEQ DYLQACRAWL
2760 2770 2780 2790 2800
AYALDQYRNS GVEIVYTPPR YSIGVSRLQS GFKKLVSPSS AVEKCIVSVS
2810 2820 2830 2840 2850
YRGNNLNGLW LGDTIYCPRH VLGKFSGDQW NDVLNLANNH EFEVTTQHGV
2860 2870 2880 2890 2900
TLNVVSRRLK GAVLILQTAV ANAETPKYKF IKANCGDSFT IACAYGGTVV
2910 2920 2930 2940 2950
GLYPVTMRSN GTIRASFLAG ACGSVGFNIE KGVVNFFYMH HLELPNALHT
2960 2970 2980 2990 3000
GTDLMGEFYG GYVDEEVAQR VPPDNLVTNN IVAWLYAAII SVKESSFSLP
3010 3020 3030 3040 3050
KWLESTTVSV DDYNKWAGDN GFTPFSTSTA ITKLSAITGV DVCKLLRTIM
3060 3070 3080 3090 3100
VKNSQWGGDP ILGQYNFEDE LTPESVFNQI GGVRLQSSFV RKATSWFWSR
3110 3120 3130 3140 3150
CVLACFLFVL CAIVLFTAVP LKFYVYAAVI LLMAVLFISF TVKHVMAYMD
3160 3170 3180 3190 3200
TFLLPTLITV IIGVCAEVPF IYNTLISQVV IFLSQWYDPV VFDTMVPWMF
3210 3220 3230 3240 3250
LPLVLYTAFK CVQGCYMNSF NTSLLMLYQF VKLGFVIYTS SNTLTAYTEG
3260 3270 3280 3290 3300
NWELFFELVH TTVLANVSSN SLIGLFVFKC AKWMLYYCNA TYLNNYVLMA
3310 3320 3330 3340 3350
VMVNCIGWLC TCYFGLYWWV NKVFGLTLGK YNFKVSVDQY RYMCLHKINP
3360 3370 3380 3390 3400
PKTVWEVFST NILIQGIGGD RVLPIATVQA KLSDVKCTTV VLMQLLTKLN
3410 3420 3430 3440 3450
VEANSKMHVY LVELHNKILA SDDVGECMDN LLGMLITLFC IDSTIDLSEY
3460 3470 3480 3490 3500
CDDILKRSTV LQSVTQEFSH IPSYAEYERA KNLYEKVLVD SKNGGVTQQE
3510 3520 3530 3540 3550
LAAYRKAANI AKSVFDRDLA VQKKLDSMAE RAMTTMYKEA RVTDRRAKLV
3560 3570 3580 3590 3600
SSLHALLFSM LKKIDSEKLN VLFDQASSGV VPLATVPIVC SNKLTLVIPD
3610 3620 3630 3640 3650
PETWVKCVEG VHVTYSTVVW NIDTVIDADG TELHPTSTGS GLTYCISGAN
3660 3670 3680 3690 3700
IAWPLKVNLT RNGHNKVDVV LQNNELMPHG VKTKACVAGV DQAHCSVESK
3710 3720 3730 3740 3750
CYYTNISGNS VVAAITSSNP NLKVASFLNE AGNQIYVDLD PPCKFGMKVG
3760 3770 3780 3790 3800
VKVEVVYLYF IKNTRSIVRG MVLGAISNVV VLQSKGHETE EVDAVGILSL
3810 3820 3830 3840 3850
CSFAVDPADT YCKYVAAGNQ PLGNCVKMLT VHNGSGFAIT SKPSPTPDQD
3860 3870 3880 3890 3900
SYGGASVCLY CRAHIAHPGS VGNLDGRCQF KGSFVQIPTT EKDPVGFCLR
3910 3920 3930 3940 3950
NKVCTVCQCW IGYGCQCDSL RQPKSSVQSV AGASDFDKNY LNGYGVAVRL
G
Note: Produced by conventional translation.
Isoform Replicase polyprotein 1ab (identifier: P0C6Y1-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6Y1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 105 | P → S in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 919 | K → E in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 932 | L → I in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 948 | D → G in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 967 | A → D in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 1133 | L → S in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 1388 | P → S in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 1753 | L → F in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 2561 | Q → H in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 3058 | Missing in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 3242 | N → S in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 3409 | V → A in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 3418 | I → T in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 3471 – 3472 | IP → MT in strain: Isolate Vero cell-adapted p65. | 2 | |
Natural varianti | 3751 | V → D in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 3870 | S → G in strain: Isolate Vero cell-adapted p65. | 1 | |
Natural varianti | 3935 | D → G in strain: Isolate Vero cell-adapted p65. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94356 Genomic RNA Translation: AAA46223.1 M95169 Genomic RNA Translation: AAA70233.1 DQ001339 Genomic RNA Translation: AAY24431.1 |
PIRi | A33094, VFIHB1 B33094, VFIHB2 |
RefSeqi | NP_040829.1, NC_001451.1 |
Genome annotation databases
GeneIDi | 1489740 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94356 Genomic RNA Translation: AAA46223.1 M95169 Genomic RNA Translation: AAA70233.1 DQ001339 Genomic RNA Translation: AAY24431.1 |
PIRi | A33094, VFIHB1 B33094, VFIHB2 |
RefSeqi | NP_040829.1, NC_001451.1 |
3D structure databases
SMRi | P0C6V3 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 1489740 |
Enzyme and pathway databases
BRENDAi | 3.4.22.B14, 8728 |
Family and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.40.220.10, 1 hit 3.90.70.90, 1 hit |
InterProi | View protein in InterPro IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR040795, Nsp1a_N IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043503, PLpro_palm_finger_dom_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF09401, CoV_NSP10, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF01661, Macro, 1 hit PF17896, Nsp2a_N, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | R1A_IBVB | |
Accessioni | P0C6V3Primary (citable) accession number: P0C6V3 Secondary accession number(s): P27920 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | December 2, 2020 | |
This is version 70 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families