UniProtKB - P0C6V2 (R1A_CVPPU)
Protein
Replicase polyprotein 1a
Gene
1a
Organism
Porcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV)
Status
Functioni
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).By similarity
Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1093 | For PL1-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1244 | For PL1-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1588 | For PL2-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1741 | For PL2-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 2919 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3022 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1164 – 1195 | C4-typePROSITE-ProRule annotationAdd BLAST | 32 | |
Zinc fingeri | 3937 – 3953 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 3979 – 3992 | By similarityAdd BLAST | 14 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: InterPro
- RNA binding Source: UniProtKB-KW
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB-EC
- transferase activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- induction by virus of host autophagy Source: UniProtKB-KW
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- suppression by virus of host IRF3 activity Source: UniProtKB-KW
- viral genome replication Source: InterPro
- viral protein processing Source: InterPro
Keywordsi
Enzyme and pathway databases
BRENDAi | 3.4.22.B14, 4985 |
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1aShort name: pp1a Alternative name(s): ORF1a polyprotein Cleaved into the following 11 chains: Alternative name(s): p9 Alternative name(s): p87 Alternative name(s): PL1-PRO/PL2-PRO PLP1/PLP2 Papain-like proteinases 1/2 p195 Alternative name(s): Peptide HD2 Alternative name(s): M-PRO nsp5 p34 Alternative name(s): p5 Alternative name(s): p23 Alternative name(s): p12 Alternative name(s): Growth factor-like peptide Short name: GFL p14 |
Gene namesi | ORF Names:1a |
Organismi | Porcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV) |
Taxonomic identifieri | 11151 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Alphacoronavirus › Tegacovirus › |
Virus hosti | Sus scrofa (Pig) [TaxID: 9823] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1896 – 1916 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1995 – 2015 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2033 – 2053 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2401 – 2421 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2467 – 2487 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2497 – 2517 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2538 – 2558 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2666 – 2686 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2695 – 2715 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2721 – 2741 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2746 – 2766 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3187 – 3207 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3217 – 3237 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3242 – 3262 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3280 – 3300 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3313 – 3333 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3347 – 3367 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3371 – 3391 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3394 – 3414 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- host cell membrane Source: UniProtKB-SubCell
- host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000338302 | 1 – 4017 | Replicase polyprotein 1aAdd BLAST | 4017 | |
ChainiPRO_0000338303 | 1 – 110 | Non-structural protein 1By similarityAdd BLAST | 110 | |
ChainiPRO_0000338304 | 111 – 879 | Non-structural protein 2By similarityAdd BLAST | 769 | |
ChainiPRO_0000338305 | 880 – 2388 | Non-structural protein 3By similarityAdd BLAST | 1509 | |
ChainiPRO_0000338306 | 2389 – 2878 | Non-structural protein 4By similarityAdd BLAST | 490 | |
ChainiPRO_0000338307 | 2879 – 3180 | 3C-like proteinaseAdd BLAST | 302 | |
ChainiPRO_0000338308 | 3181 – 3474 | Non-structural protein 6By similarityAdd BLAST | 294 | |
ChainiPRO_0000338309 | 3475 – 3557 | Non-structural protein 7By similarityAdd BLAST | 83 | |
ChainiPRO_0000338310 | 3558 – 3752 | Non-structural protein 8By similarityAdd BLAST | 195 | |
ChainiPRO_0000338311 | 3753 – 3863 | Non-structural protein 9Add BLAST | 111 | |
ChainiPRO_0000338312 | 3864 – 3998 | Non-structural protein 10By similarityAdd BLAST | 135 | |
ChainiPRO_0000338313 | 3999 – 4017 | Non-structural protein 11Sequence analysisAdd BLAST | 19 |
Post-translational modificationi
Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO is autocatalytically processed.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 110 – 111 | Cleavage; by PL1-PROBy similarity | 2 | |
Sitei | 879 – 880 | Cleavage; by PL1-PROBy similarity | 2 | |
Sitei | 2388 – 2389 | Cleavage; by PL2-PROBy similarity | 2 | |
Sitei | 2878 – 2879 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3180 – 3181 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3474 – 3475 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3557 – 3558 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3752 – 3753 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3863 – 3864 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3998 – 3999 | Cleavage; by 3CL-PROBy similarity | 2 |
Proteomic databases
PRIDEi | P0C6V2 |
Interactioni
Subunit structurei
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
By similarityStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0C6V2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0C6V2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1055 – 1299 | Peptidase C16 1PROSITE-ProRule annotationAdd BLAST | 245 | |
Domaini | 1318 – 1489 | MacroPROSITE-ProRule annotationAdd BLAST | 172 | |
Domaini | 1550 – 1803 | Peptidase C16 2PROSITE-ProRule annotationAdd BLAST | 254 | |
Domaini | 2879 – 3180 | Peptidase C30PROSITE-ProRule annotationAdd BLAST | 302 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1896 – 2053 | HD1Add BLAST | 158 | |
Regioni | 2401 – 2766 | HD2Add BLAST | 366 | |
Regioni | 3187 – 3414 | HD3Add BLAST | 228 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 932 – 1042 | Glu-richAdd BLAST | 111 |
Domaini
The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.
Sequence similaritiesi
Belongs to the coronaviruses polyprotein 1ab family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1164 – 1195 | C4-typePROSITE-ProRule annotationAdd BLAST | 32 | |
Zinc fingeri | 3937 – 3953 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 3979 – 3992 | By similarityAdd BLAST | 14 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.370, 1 hit 2.30.30.1000, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.40.220.10, 1 hit 3.90.70.90, 2 hits |
InterProi | View protein in InterPro IPR032039, A-CoV_nsp1 IPR038634, A-CoV_nsp1_sf IPR043613, CoV_NSP2_C IPR043615, CoV_NSP2_N IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043503, PLpro_palm_finger_dom_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16688, CNV-Replicase_N, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 2 hits PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 2 hits PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 2 hits SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform Replicase polyprotein 1a (identifier: P0C6V2-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSKQFKILV NEDYQVNVPS LPIRDVLQEI KYCYRNGFEG YVFVPEYCRD
60 70 80 90 100
LVDCDRKDHY VIGVLGNGVS DLKPVLLTEP SVMLQGFIVR ANCNGVLEDF
110 120 130 140 150
DLKIARTGRG AIYVDQYMCG ADGKPVIEGD FKDYFGDEDI IEFEGEEYHC
160 170 180 190 200
AWTTVRDEKP LNQQTLFTIQ EIQYNLDIPH KLPNCATRHV APPVKKNSKI
210 220 230 240 250
VLSEDYKKLY DIFGSPFMGN GDCLSKCFDT LHFIAATLRC PCGSESSGVG
260 270 280 290 300
DWTGFKTACC GLSGKVKGVT LGDIKPGDAV VTSMSAGKGV KFFANCVLQY
310 320 330 340 350
AGDVEGVSIW KVIKTFTVDE TVCTPGFEGE LNDFIKPESK SLVACSVKRA
360 370 380 390 400
FITGDIDDAV HDCIITGKLD LSTNLFGNVG LLFKKTPWFV QKCGALFVDA
410 420 430 440 450
WKVVEELCGS LTLTYKQIYE VVASLCTSAF TIVNYKPTFV VPDNRVKDLV
460 470 480 490 500
DKCVKVLVKA FDVFTQIITI AGIEAKCFVL GAKYLLFNNA LVKLVSVKIL
510 520 530 540 550
GKKQKGLECA FFATSLVGAT VNVTPKRTET ATISLNKVDD VVAPGEGYIV
560 570 580 590 600
IVGDMAFYKS GEYYFMMSSP NFVLTNNVFK AVKVPSYDIV YDVDNDTKSK
610 620 630 640 650
MIAKLGSSFE YDGDIDAAIV KVNELLIEFR QQSLCFRAFK DDKSIFVEAY
660 670 680 690 700
FKKYKMPACL AKHIGLWNII KKDSCKRGFL NLFNHLNELE DIKETNIQAI
710 720 730 740 750
KNILCPDPLL DLDYGAIWYN CMPGCSDPSV LGSVQLLIGN GVKVVCDGCK
760 770 780 790 800
GFANQLSKGY NKLCNAARND IEIGGIPFST FKTPTNTFIE MTDAIYSVIE
810 820 830 840 850
QGKALSFRDA DVPVVDNGTI STADWSEPIL LEPAEYVKPK NNGNVIVIAG
860 870 880 890 900
YTFYKDEDEH FYPYGFGKIV QRMYNKMGGG DKTVSFSEEV DVQEIAPVTR
910 920 930 940 950
VKLEFEFDNE IVTGVLERAI GTRYKFTGTT WEEFEESISE ELDAIFDTLA
960 970 980 990 1000
NQGVELEGYF IYDTCGGFDI KNPDGIMISQ YDINITADEK SEVSASSEEE
1010 1020 1030 1040 1050
EVESVEEDPE NEIVEASEGA EGTSSQEEVE TVEVADITST EEDVDIVEVS
1060 1070 1080 1090 1100
AKDDPWAAAV DVQEAEQFNP SLPPFKTTNL NGKIILKQGD NNCWINACCY
1110 1120 1130 1140 1150
QLQAFDFFNN EAWEKFKKGD VMDFVNLCYA ATTLARGHSG DAEYLLELML
1160 1170 1180 1190 1200
NDYSTAKIVL AAKCGCGEKE IVLERAVFKL TPLKESFNYG VCGDCMQVNT
1210 1220 1230 1240 1250
CRFLSVEGSG VFVHDILSKQ TPEAMFVVKP VMHAVYTGTT QNGHYMVDDI
1260 1270 1280 1290 1300
EHGYCVDGMG IKPLKKRCYT STLFINANVM TRAEKPKQEF KVEKVEQQPI
1310 1320 1330 1340 1350
VEENKSSIEK EEIQSPKNDD LILPFYKAGK LSFYQGALDV LINFLEPDVI
1360 1370 1380 1390 1400
VNAANGDLKH MGGVARAIDV FTGGKLTERS KDYLKKNKSI APGNAVFFEN
1410 1420 1430 1440 1450
VIEHLSVLNA VGPRNGDSRV EAKLCNVYKA IAKCEGKILT PLISVGIFNV
1460 1470 1480 1490 1500
RLETSLQCLL KTVNDRGLNV FVYTDQERQT IENFFSCSIP VNVTEDNVNH
1510 1520 1530 1540 1550
ERVSVSFDKT YGEQLKGTVV IKDKDVTNQL PSAFDVGQKV IKAIDIDWQA
1560 1570 1580 1590 1600
HYGFRDAAAF SASSHDAYKF EVVTHSNFIV HKQTDNNCWI NAICLALQRL
1610 1620 1630 1640 1650
KPQWKFPGVR GLWNEFLERK TQGFVHMLYH ISGVKKGEPG DAELMLHKLG
1660 1670 1680 1690 1700
DLMDNDCEII VTHTTACDKC AKVEKFVGPV VAAPLAIHGT DETCVHGVSV
1710 1720 1730 1740 1750
NVKVTQIKGT VAITSLIGPI IGEVLEATGY ICYSGSNRNG HYTYYDNRNG
1760 1770 1780 1790 1800
LVVDAEKAYH FNRDLLQVTT AIASNFVVKK PQAEERPKNC AFNKVAASPK
1810 1820 1830 1840 1850
IVQEQKLLAI ESGANYALTE FGRYADMFFM AGDKILRLLL EVFKYLLVLF
1860 1870 1880 1890 1900
MCLRSTKMPK VKVKPPLAFK DFGAKVRTLN YMRQLNKPSV WRYAKLVLLL
1910 1920 1930 1940 1950
IAIYNFFYLF VSIPVVHKLT CNGAVQAYKN SSFIKSAVCG NSILCKACLA
1960 1970 1980 1990 2000
SYDELADFQH LQVTWDFKSD PLWNRLVQLS YFAFLAVFGN NYVRCFLMYF
2010 2020 2030 2040 2050
VSQYLNLWLS YFGYVEYSWF LHVVNFESIS AEFVIVVIVV KAVLALKHIV
2060 2070 2080 2090 2100
FACSNPSCKT CSRTARQTRI PIQVVVNGSM KTVYVHANGT GKFCKKHNFY
2110 2120 2130 2140 2150
CKNCDSYGFE NTFICDEIVR DLSNSVKQTV YATDRSHQEV TKVECSDGFY
2160 2170 2180 2190 2200
RFYVGDEFTS YDYDVKHKKY SSQEVLKSML LLDDFIVYSP SGSALANVRN
2210 2220 2230 2240 2250
ACVYFSQLIG KPIKIVNSDL LEDLSVDFKG ALFNAKKNVI KNSFNVDVSE
2260 2270 2280 2290 2300
CKNLDECYRA CNLNVSFSTF EMAVNNAHRF GILITDRSFN NFWPSKVKPG
2310 2320 2330 2340 2350
SSGVSAMDIG KCMTSDAKIV NAKVLTQRGK SVVWLSQDFA ALSSTAQKVL
2360 2370 2380 2390 2400
VKTFVEEGVN FSLTFNAVGS DDDLPYERFT ESVSPKSGSG FFDVITQLKQ
2410 2420 2430 2440 2450
IVILVFVFIF ICGLCSVYSV ATQSYIESAE GYDYMVIKNG IVQPFDDTIS
2460 2470 2480 2490 2500
CVHNTYKGFG DWFKAKYGFI PTFGKSCPIV VGTVFDLENM RPIPDVPAYV
2510 2520 2530 2540 2550
SIVGRSLVFA INAAFGVTNM CYDHTGNAVS KDSYFDTCVF NTACTTLTGL
2560 2570 2580 2590 2600
GGTIVYCAKQ GLVEGAKLYS DLMPDYYYEH ASGNMVKLPA IIRGLGLRFV
2610 2620 2630 2640 2650
KTQATTYCRV GECIDSKAGF CFGGDNWFVY DNEFGNGYIC GNSVLGFFKN
2660 2670 2680 2690 2700
VFKLFNSNMS VVATSGAMLV NIIIACLAIA MCYGVLKFKK IFGDCTFLIV
2710 2720 2730 2740 2750
MIIVTLVVNN VSYFVTQNTF FMIIYAIVYY FITRKLAYPG ILDAGFIIAY
2760 2770 2780 2790 2800
INMAPWYVIT AYILVFLYDS LPSLFKLKVS TNLFEGDKFV GNFESAAMGT
2810 2820 2830 2840 2850
FVIDMRSYET IVNSTSIARI KSYANSFNKY KYYTGSMGEA DYRMACYAHL
2860 2870 2880 2890 2900
GKALMDYSVN RTDMLYTPPT VSVNSTLQSG LRKMAQPSGL VEPCIVRVSY
2910 2920 2930 2940 2950
GNNVLNGLWL GDEVICPRHV IASDTTRVIN YENEMSSVRL HNFSVSKNNV
2960 2970 2980 2990 3000
FLGVVSARYK GVNLVLKVNQ VNPNTPEHKF KSIKAGESFN ILACYEGCPG
3010 3020 3030 3040 3050
SVYGVNMRSQ GTIKGSFIAG TCGSVGYVLE NGILYFVYMH HLELGNGSHV
3060 3070 3080 3090 3100
GSNFEGEMYG GYEDQPSMQL EGTNVMSSDN VVAFLYAALI NGERWFVTNT
3110 3120 3130 3140 3150
SMSLESYNTW AKTNSFTELS STDAFSMLAA KTGQSVEKLL DSIVRLNKGF
3160 3170 3180 3190 3200
GGRTILSYGS LCDEFTPTEV IRQMYGVNLQ AGKVKSFFYP IMTAMTILFA
3210 3220 3230 3240 3250
FWLEFFMYTP FTWINPTFVS IVLAVTTLIS TVFVSGIKHK MLFFMSFVLP
3260 3270 3280 3290 3300
SVILVTAHNL FWDFSYYESL QSIVENTNTM FLPVDMQGVM LTVFCFIVFV
3310 3320 3330 3340 3350
TYSVRFFTCK QSWFSLAVTT ILVIFNMVKI FGTSDEPWTE NQIAFCFVNM
3360 3370 3380 3390 3400
LTMIVSLTTK DWMVVIASYR IAYYIVVCVM PSAFVSDFGF MKCISIVYMA
3410 3420 3430 3440 3450
CGYLFCCYYG ILYWVNRFTC MTCGVYQFTV SAAELKYMTA NNLSAPKNAY
3460 3470 3480 3490 3500
DAMILSAKLI GVGGKRNIKI STVQSKLTEM KCTNVVLLGL LSKMHVESNS
3510 3520 3530 3540 3550
KEWNYCVGLH NEINLCDDPE IVLEKLLALI AFFLSKHNTC DLSELIESYF
3560 3570 3580 3590 3600
ENTTILQSVA SAYAALPSWI ALEKARADLE EAKKNDVSPQ ILKQLTKAFN
3610 3620 3630 3640 3650
IAKSDFEREA SVQKKLDKMA EQAAASMYKE ARAVDRKSKI VSAMHSLLFG
3660 3670 3680 3690 3700
MLKKLDMSSV NTIIDQARNG VLPLSIIPAA SATRLVVITP SLEVFSKIRQ
3710 3720 3730 3740 3750
ENNVHYAGAI WTIVEVKDAN GSHVHLKEVT AANELNLTWP LSITCERTTK
3760 3770 3780 3790 3800
LQNNEIMPGK LKERAVRASA TLDGEAFGSG KALMASESGK SFMYAFIASD
3810 3820 3830 3840 3850
NNLKYVKWES NNDIIPIELE APLRFYVDGA NGPEVKYLYF VKNLNTLRRG
3860 3870 3880 3890 3900
AVLGYIGATV RLQAGKPTEH PSNSSLLTLC AFSPDPAKAY VDAVKRGMQP
3910 3920 3930 3940 3950
VNNCVKMLSN GAGNGMAVTN GVEANTQQDS YGGASVCIYC RCHVEHPAID
3960 3970 3980 3990 4000
GLCRYKGKFV QIPTGTQDPI RFCIENEVCV VCGCWLNNGC MCDRTSMQSF
4010
TVDQSYLNEC GVLVQLD
Note: Produced by conventional translation.
Isoform Replicase polyprotein 1ab (identifier: P0C6Y5-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6Y5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 572 | F → S in strain: Isolate Purdue-115. | 1 | |
Natural varianti | 1041 | E → D in strain: Isolate Purdue-115. | 1 | |
Natural varianti | 2375 | P → T in strain: Isolate Purdue-115. | 1 | |
Natural varianti | 2381 | E → Q in strain: Isolate Purdue-115. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z34093 mRNA Translation: CAA83979.1 AJ271965 Genomic RNA Translation: CAB91144.1 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z34093 mRNA Translation: CAA83979.1 AJ271965 Genomic RNA Translation: CAB91144.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1LVO | X-ray | 1.96 | A/B/C/D/E/F | 2879-3180 | [»] | |
1P9U | X-ray | 2.37 | A/B/C/D/E/F | 2879-3180 | [»] | |
2AMP | X-ray | 2.70 | A/B | 2879-3180 | [»] | |
3MP2 | X-ray | 2.50 | A | 1071-1281 | [»] | |
3ZBD | X-ray | 1.49 | A/B | 1-105 | [»] | |
4F49 | X-ray | 2.25 | A/B/C/D | 2879-3181 | [»] | |
6IVD | X-ray | 1.98 | A/B | 1-105 | [»] | |
SMRi | P0C6V2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PRIDEi | P0C6V2 |
Enzyme and pathway databases
BRENDAi | 3.4.22.B14, 4985 |
Miscellaneous databases
EvolutionaryTracei | P0C6V2 |
Family and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.370, 1 hit 2.30.30.1000, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.40.220.10, 1 hit 3.90.70.90, 2 hits |
InterProi | View protein in InterPro IPR032039, A-CoV_nsp1 IPR038634, A-CoV_nsp1_sf IPR043613, CoV_NSP2_C IPR043615, CoV_NSP2_N IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043503, PLpro_palm_finger_dom_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16688, CNV-Replicase_N, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 2 hits PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 2 hits PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 2 hits SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | R1A_CVPPU | |
Accessioni | P0C6V2Primary (citable) accession number: P0C6V2 Secondary accession number(s): Q9IW06 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | December 2, 2020 | |
This is version 81 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families