UniProtKB - P0C6V0 (R1A_CVMA5)
Protein
Replicase polyprotein 1a
Gene
1a
Organism
Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
Status
Functioni
The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).By similarity
Catalytic activityi
- TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. EC:3.4.22.69
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1121 | For PL1-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1272 | For PL1-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1716 | For PL2-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1873 | For PL2-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3374 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3478 | For 3CL-PRO activity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1198 – 1226 | C4-type 1PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 1794 – 1830 | C4-type 2PROSITE-ProRule annotationAdd BLAST | 37 | |
Zinc fingeri | 4391 – 4407 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4433 – 4446 | By similarityAdd BLAST | 14 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: InterPro
- single-stranded RNA binding Source: InterPro
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB-EC
- zinc ion binding Source: InterPro
GO - Biological processi
- induction by virus of catabolism of host mRNA Source: UniProtKB-KW
- induction by virus of host autophagy Source: UniProtKB-KW
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- self proteolysis Source: CACAO
- suppression by virus of host IRF3 activity Source: UniProtKB-KW
- suppression by virus of host ISG15 activity Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
- viral genome replication Source: InterPro
- viral protein processing Source: InterPro
Keywordsi
Enzyme and pathway databases
BRENDAi | 3.4.22.B14, 3442 |
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1aShort name: pp1a Alternative name(s): ORF1a polyprotein Cleaved into the following 11 chains: Alternative name(s): p28 Alternative name(s): p65 Alternative name(s): PL1-PRO/PL2-PRO PL1/PL2 Papain-like proteinases 1/2 p210 Alternative name(s): Peptide HD2 p44 Alternative name(s): M-PRO nsp5 p27 Alternative name(s): p10 Alternative name(s): p22 Alternative name(s): p12 Alternative name(s): Growth factor-like peptide Short name: GFL p15 |
Gene namesi | ORF Names:1a |
Organismi | Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus) |
Taxonomic identifieri | 11142 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Embecovirus › |
Virus hosti | Mus musculus (Mouse) [TaxID: 10090] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 2286 – 2306 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2314 – 2334 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2400 – 2420 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2442 – 2462 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2625 – 2645 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2847 – 2867 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3096 – 3116 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3118 – 3138 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3150 – 3170 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3177 – 3197 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3202 – 3222 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3644 – 3664 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3674 – 3694 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3699 – 3719 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3742 – 3762 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3769 – 3789 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3796 – 3816 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3840 – 3860 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- cytoplasmic viral factory Source: UniProtKB
- host cell membrane Source: UniProtKB-SubCell
- host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 3331 | F → A, H or W: No effect. 1 Publication | 1 | |
Mutagenesisi | 3332 | L → I or S: No processing between peptide HD2 and 3CL-PRO. 1 Publication | 1 | |
Mutagenesisi | 3333 | Q → A, K or R: No processing between peptide HD2 and 3CL-PRO. 1 Publication | 1 | |
Mutagenesisi | 3334 | S → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 3334 | S → C: No processing between peptide HD2 and 3CL-PRO. 1 Publication | 1 | |
Mutagenesisi | 3335 | G → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 3335 | G → P: No processing between peptide HD2 and 3CL-PRO. 1 Publication | 1 | |
Mutagenesisi | 3336 | I → L: No effect. 1 Publication | 1 | |
Mutagenesisi | 3478 | C → A: Complete loss of 3CL-PRO activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000338278 | 1 – 4468 | Replicase polyprotein 1aAdd BLAST | 4468 | |
ChainiPRO_0000338279 | 1 – 247 | Non-structural protein 1CuratedAdd BLAST | 247 | |
ChainiPRO_0000338280 | 248 – 832 | Non-structural protein 2CuratedAdd BLAST | 585 | |
ChainiPRO_0000338281 | 833 – 2837 | Non-structural protein 3CuratedAdd BLAST | 2005 | |
ChainiPRO_0000338282 | 2838 – 3333 | Non-structural protein 4Add BLAST | 496 | |
ChainiPRO_0000338283 | 3334 – 3635 | 3C-like proteinaseAdd BLAST | 302 | |
ChainiPRO_0000338284 | 3636 – 3921 | Non-structural protein 6CuratedAdd BLAST | 286 | |
ChainiPRO_0000338285 | 3922 – 4013 | Non-structural protein 7Add BLAST | 92 | |
ChainiPRO_0000338286 | 4014 – 4207 | Non-structural protein 8Add BLAST | 194 | |
ChainiPRO_0000338287 | 4208 – 4317 | Non-structural protein 9Add BLAST | 110 | |
ChainiPRO_0000338288 | 4318 – 4454 | Non-structural protein 10Add BLAST | 137 | |
ChainiPRO_0000338289 | 4455 – 4468 | Non-structural protein 11Sequence analysisAdd BLAST | 14 |
Post-translational modificationi
Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 247 – 248 | Cleavage; by PL1-PROCurated | 2 | |
Sitei | 832 – 833 | Cleavage; by PL1-PROCurated | 2 | |
Sitei | 2837 – 2838 | Cleavage; by PL2-PRO | 2 | |
Sitei | 3333 – 3334 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3635 – 3636 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3921 – 3922 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 4013 – 4014 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 4207 – 4208 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 4317 – 4318 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 4454 – 4455 | Cleavage; by 3CL-PRO | 2 |
Interactioni
Subunit structurei
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
By similarityStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0C6V0 |
SMRi | P0C6V0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1084 – 1333 | Peptidase C16 1PROSITE-ProRule annotationAdd BLAST | 250 | |
Domaini | 1323 – 1482 | MacroPROSITE-ProRule annotationAdd BLAST | 160 | |
Domaini | 1678 – 1937 | Peptidase C16 2PROSITE-ProRule annotationAdd BLAST | 260 | |
Domaini | 3334 – 3635 | Peptidase C30PROSITE-ProRule annotationAdd BLAST | 302 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2225 – 2645 | HD1Add BLAST | 421 | |
Regioni | 2847 – 3222 | HD2Add BLAST | 376 | |
Regioni | 3526 – 3860 | HD3Add BLAST | 335 |
Domaini
The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.
Sequence similaritiesi
Belongs to the coronaviruses polyprotein 1ab family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1198 – 1226 | C4-type 1PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 1794 – 1830 | C4-type 2PROSITE-ProRule annotationAdd BLAST | 37 | |
Zinc fingeri | 4391 – 4407 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4433 – 4446 | By similarityAdd BLAST | 14 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.40.220.10, 1 hit 3.90.70.90, 1 hit |
InterProi | View protein in InterPro IPR022570, B-CoV_NSP1 IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR032592, NSP3_NAR_bCoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR002705, Pept_C30/C16_B_coronavir IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF11963, DUF3477, 2 hits PF01661, Macro, 1 hit PF01831, Peptidase_C16, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform Replicase polyprotein 1a (identifier: P0C6V0-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKMGKYGLG FKWAPEFPWM LPNASEKLGN PERSEEDGFC PSAAQEPKVK
60 70 80 90 100
GKTLVNHVRV NCSRLPALEC CVQSAIIRDI FVDEDPQKVE ASTMMALQFG
110 120 130 140 150
SAVLVKPSKR LSIQAWTNLG VLPKTAAMGL FKRVCLCNTR ECSCDAHVAF
160 170 180 190 200
HLFTVQPDGV CLGNGRFIGW FVPVTAIPEY AKQWLQPWSI LLRKGGNKGS
210 220 230 240 250
VTSGHFRRAV TMPVYDFNVE DACEEVHLNP KGKYSCKAYA LLKGYRGVKP
260 270 280 290 300
ILFVDQYGCD YTGCLAKGLE DYGDLTLSEM KELFPVWRDS LDSEVLVAWH
310 320 330 340 350
VDRDPRAAMR LQTLATVRCI DYVGQPTEDV VDGDVVVREP AHLLAANAIV
360 370 380 390 400
KRLPRLVETM LYTDSSVTEF CYKTKLCECG FITQFGYVDC CGDTCDFRGW
410 420 430 440 450
VAGNMMDGFP CPGCTKNYMP WELEAQSSGV IPEGGVLFTQ STDTVNRESF
460 470 480 490 500
KLYGHAVVPF GSAVYWSPCP GMWLPVIWSS VKSYSGLTYT GVVGCKAIVQ
510 520 530 540 550
ETDAICRSLY MDYVQHKCGN LEQRAILGLD DVYHRQLLVN RGDYSLLLEN
560 570 580 590 600
VDLFVKRRAE FACKFATCGD GLVPLLLDGL VPRSYYLIKS GQAFTSMMVN
610 620 630 640 650
FSHEVTDMCM DMALLFMHDV KVATKYVKKV TGKLAVRFKA LGVAVVRKIT
660 670 680 690 700
EWFDLAVDIA ASAAGWLCYQ LVNGLFAVAN GVITFVQEVP ELVKNFVDKF
710 720 730 740 750
KAFFKVLIDS MSVSILSGLT VVKTASNRVC LAGSKVYEVV QKSLSAYVMP
760 770 780 790 800
VGCSEATCLV GEIEPAVFED DVVDVVKAPL TYQGCCKPPT SFEKICIVDK
810 820 830 840 850
LYMAKCGDQF YPVVVDNDTV GVLDQCWRFP CAGKKVEFND KPKVRKIPST
860 870 880 890 900
RKIKITFALD ATFDSVLSKA CSEFEVDKDV TLDELLDVVL DAVESTLSPC
910 920 930 940 950
KEHDVIGTKV CALLDRLAGD YVYLFDEGGD EVIAPRMYCS FSAPDDEDCV
960 970 980 990 1000
AADVVDADEN QDDDAEDSAV LVADTQEEDG VAKGQVEADS EICVAHTGSQ
1010 1020 1030 1040 1050
EELAEPDAVG SQTPIASAEE TEVGEASDRE GIAEAKATVC ADAVDACPDQ
1060 1070 1080 1090 1100
VEAFEIEKVE DSILDELQTE LNAPADKTYE DVLAFDAVCS EALSAFYAVP
1110 1120 1130 1140 1150
SDETHFKVCG FYSPAIERTN CWLRSTLIVM QSLPLEFKDL EMQKLWLSYK
1160 1170 1180 1190 1200
AGYDQCFVDK LVKSVPKSII LPQGGYVADF AYFFLSQCSF KAYANWRCLE
1210 1220 1230 1240 1250
CDMELKLQGL DAMFFYGDVV SHMCKCGNSM TLLSADIPYT LHFGVRDDKF
1260 1270 1280 1290 1300
CAFYTPRKVF RAACAVDVND CHSMAVVEGK QIDGKVVTKF IGDKFDFMVG
1310 1320 1330 1340 1350
YGMTFSMSPF ELAQLYGSCI TPNVCFVKGD VIKVVRLVNA EVIVNPANGR
1360 1370 1380 1390 1400
MAHGAGVAGA IAEKAGSAFI KETSDMVKAQ GVCQVGECYE SAGGKLCKKV
1410 1420 1430 1440 1450
LNIVGPDARG HGKQCYSLLE RAYQHINKCD NVVTTLISAG IFSVPTDVSL
1460 1470 1480 1490 1500
TYLLGVVTKN VILVSNNQDD FDVIEKCQVT SVAGTKALSL QLAKNLCRDV
1510 1520 1530 1540 1550
KFVTNACSSL FSESCFVSSY DVLQEVEALR HDIQLDDDAR VFVQANMDCL
1560 1570 1580 1590 1600
PTDWRLVNKF DSVDGVRTIK YFECPGGIFV SSQGKKFGYV QNGSFKEASV
1610 1620 1630 1640 1650
SQIRALLANK VDVLCTVDGV NFRSCCVAEG EVFGKTLGSV FCDGINVTKV
1660 1670 1680 1690 1700
RCSAIYKGKV FFQYSDLSEA DLVAVKDAFG FDEPQLLKYY TMLGMCKWPV
1710 1720 1730 1740 1750
VVCGNYFAFK QSNNNCYINV ACLMLQHLSL KFPKWQWQEA WNEFRSGKPL
1760 1770 1780 1790 1800
RFVSLVLAKG SFKFNEPSDS IDFMRVVLRE ADLSGATCNL EFVCKCGVKQ
1810 1820 1830 1840 1850
EQRKGVDAVM HFGTLDKGDL VRGYNIACTC GSKLVHCTQF NVPFLICSNT
1860 1870 1880 1890 1900
PEGRKLPDDV VAANIFTGGS VGHYTHVKCK PKYQLYDACN VNKVSEAKGN
1910 1920 1930 1940 1950
FTDCLYLKNL KQTFSSVLTT FYLDDVKCVE YKPDLSQYYC ESGKYYTKPI
1960 1970 1980 1990 2000
IKAQFRTFEK VDGVYTNFKL VGHSIAEKLN AKLGFDCNSP FVEYKITEWP
2010 2020 2030 2040 2050
TATGDVVLAS DDLYVSRYSS GCITFGKPVV WLGHEEASLK SLTYFNRPSV
2060 2070 2080 2090 2100
VCENKFNVLP VDVSEPTDKG PVPAAVLVTG VPGADASAGA GIAKEQKACA
2110 2120 2130 2140 2150
SASVEDQVVT EVRQEPSVSA ADVKEVKLNG VKKPVKVEGS VVVNDPTSET
2160 2170 2180 2190 2200
KVVKSLSIVD VYDMFLTGCK YVVWTANELS RLVNSPTVRE YVKWGMGKIV
2210 2220 2230 2240 2250
TPAKLLLLRD EKQEFVAPKV VKAKAIACYC AVKWFLLYCF SWIKFNTDNK
2260 2270 2280 2290 2300
VIYTTEVASK LTFKLCCLAF KNALQTFNWS VVSRGFFLVA TVFLLWFNFL
2310 2320 2330 2340 2350
YANVILSDFY LPNIGPLPTF VGQIVAWFKT TFGVSTICDF YQVTDLGYRS
2360 2370 2380 2390 2400
SFCNGSMVCE LCFSGFDMLD NYDAINVVQH VVDRRLSFDY ISLFKLVVEL
2410 2420 2430 2440 2450
VIGYSLYTVC FYPLFVLIGM QLLTTWLPEF FMLETMHWSA RLFVFVANML
2460 2470 2480 2490 2500
PAFTLLRFYI VVTAMYKVYC LCRHVMYGCS KPGCLFCYKR NRSVRVKCST
2510 2520 2530 2540 2550
VVGGSLRYYD VMANGGTGFC TKHQWNCLNC NSWKPGNTFI THEAAADLSK
2560 2570 2580 2590 2600
ELKRPVNPTD SAYYSVTEVK QVGCSMRLFY ERDGQRVYDD VNASLFVDMN
2610 2620 2630 2640 2650
GLLHSKVKGV PETHVVVVEN EADKAGFLGA AVFYAQSLYR PMLMVEKKLI
2660 2670 2680 2690 2700
TTANTGLSVS RTMFDLYVDS LLNVLDVDRK SLTSFVNAAH NSLKEGVQLE
2710 2720 2730 2740 2750
QVMDTFIGCA RRKCAIDSDV ETKSITKSVM SAVNAGVDFT DESCNNLVPT
2760 2770 2780 2790 2800
YVKSDTIVAA DLGVLIQNNA KHVQANVAKA ANVACIWSVD AFNQLSADLQ
2810 2820 2830 2840 2850
HRLRKACSKT GLKIKLTYNK QEANVPILTT PFSLKGGAVF SRMLQWLFVA
2860 2870 2880 2890 2900
NLICFIVLWA LMPTYAVHKS DMQLPLYASF KVIDNGVLRD VSVTDACFAN
2910 2920 2930 2940 2950
KFNQFDQWYE STFGLAYYRN SKACPVVVAV IDQDIGHTLF NVPTTVLRYG
2960 2970 2980 2990 3000
FHVLHFITHA FATDSVQCYT PHMQIPYDNF YASGCVLSSL CTMLAHADGT
3010 3020 3030 3040 3050
PHPYCYTGGV MHNASLYSSL APHVRYNLAS SNGYIRFPEV VSEGIVRVVR
3060 3070 3080 3090 3100
TRSMTYCRVG LCEEAEEGIC FNFNRSWVLN NPYYRAMPGT FCGRNAFDLI
3110 3120 3130 3140 3150
HQVLGGLVRP IDFFALTASS VAGAILAIIV VLAFYYLIKL KRAFGDYTSV
3160 3170 3180 3190 3200
VVINVIVWCI NFLMLFVFQV YPTLSCLYAC FYFYTTLYFP SEISVVMHLQ
3210 3220 3230 3240 3250
WLVMYGAIMP LWFCIIYVAV VVSNHALWLF SYCRKIGTEV RSDGTFEEMA
3260 3270 3280 3290 3300
LTTFMITKES YCKLKNSVSD VAFNRYLSLY NKYRYFSGKM DTAAYREAAC
3310 3320 3330 3340 3350
SQLAKAMETF NHNNGNDVLY QPPTASVTTS FLQSGIVKMV SPTSKVEPCI
3360 3370 3380 3390 3400
VSVTYGNMTL NGLWLDDKVY CPRHVICSSA DMTDPDYPNL LCRVTSSDFC
3410 3420 3430 3440 3450
VMSGRMSLTV MSYQMQGCQL VLTVTLQNPN TPKYSFGVVK PGETFTVLAA
3460 3470 3480 3490 3500
YNGRPQGAFH VTLRSSHTIK GSFLCGSCGS VGYVLTGDSV RFVYMHQLEL
3510 3520 3530 3540 3550
STGCHTGTDF SGNFYGPYRD AQVVQLPVQD YTQTVNVVAW LYAAIFNRCN
3560 3570 3580 3590 3600
WFVQSDSCSL EEFNVWAMTN GFSSIKADLV LDALASMTGV TVEQVLAAIK
3610 3620 3630 3640 3650
RLHSGFQGKQ ILGSCVLEDE TPSDVYQQLA GVKLQSKRTR VIKGTCCWIL
3660 3670 3680 3690 3700
ASTFLFCSII SAFVKWTMFM YVTTHMLGVT LCALCFVSFA MLLIKHKHLY
3710 3720 3730 3740 3750
LTMYIMPVLC TFYTNYLVVY KQSFRGLAYA WLSHFVPAVD YTYMDEVLYG
3760 3770 3780 3790 3800
VVLLVAMVFV TMRSINHDVF SIMFLVGRLV SLVSMWYFGA NLEEEVLLFL
3810 3820 3830 3840 3850
TSLFGTYTWT TMLSLATAKV IAKWLAVNVL YFTDVPQIKL VLLSYLCIGY
3860 3870 3880 3890 3900
VCCCYWGILS LLNSIFRMPL GVYNYKISVQ ELRYMNANGL RPPRNSFEAL
3910 3920 3930 3940 3950
MLNFKLLGIG GVPVIEVSQI QSRLTDVKCA NVVLLNCLQH LHIASNSKLW
3960 3970 3980 3990 4000
QYCSTLHNEI LATSDLSMAF DKLAQLLVVL FANPAAVDSK CLASIEEVSD
4010 4020 4030 4040 4050
DYVRDNTVLQ ALQSEFVNMA SFVEYELAKK NLDEAKASGS ANQQQIKQLE
4060 4070 4080 4090 4100
KACNIAKSAY ERDRAVARKL ERMADLALTN MYKEARINDK KSKVVSALQT
4110 4120 4130 4140 4150
MLFSMVRKLD NQALNSILDN AVKGCVPLNA IPSLTSNTLT IIVPDKQVFD
4160 4170 4180 4190 4200
QVVDNVYVTY AGNVWHIQFI QDADGAVKQL NEIDVNSTWP LVIAANRHNE
4210 4220 4230 4240 4250
VSTVVLQNNE LMPQKLRTQV VNSGSDMNCN TPTQCYYNTT GTGKIVYAIL
4260 4270 4280 4290 4300
SDCDGLKYTK IVKEDGNCVV LELDPPCKFS VQDVKGLKIK YLYFVKGCNT
4310 4320 4330 4340 4350
LARGWVVGTL SSTVRLQAGT ATEYASNSAI LSLCAFSVDP KKTYLDYIKQ
4360 4370 4380 4390 4400
GGVPVTNCVK MLCDHAGTGM AITIKPEATT NQDSYGGASV CIYCRSRVEH
4410 4420 4430 4440 4450
PDVDGLCKLR GKFVQVPLGI KDPVSYVLTH DVCQVCGFWR DGSCSCVGTG
4460
SQFQSKDTNF LNGFGVQV
Note: Produced by conventional translation.
Isoform Replicase polyprotein 1ab (identifier: P0C6X9-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6X9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 287 – 288 | WR → CA (PubMed:2545027).Curated | 2 | |
Sequence conflicti | 311 | L → V (PubMed:2545027).Curated | 1 | |
Sequence conflicti | 570 | D → G (PubMed:2545027).Curated | 1 | |
Sequence conflicti | 3620 | E → EL (PubMed:9426441).Curated | 1 | |
Sequence conflicti | 3711 | T → TL (PubMed:9426441).Curated | 1 | |
Sequence conflicti | 3968 | M → V (PubMed:9426441).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 1699 | P → S in strain: Isolate C12 mutant. | 1 | |
Natural varianti | 2196 | M → K in strain: Isolate C12 mutant. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73559 Genomic RNA No translation available. AF029248 Genomic RNA Translation: AAB86820.1 M27198 Genomic RNA Translation: AAA74011.1 |
PIRi | A32440 S15760 |
RefSeqi | NP_045298.1, NC_001846.1 |
Genome annotation databases
GeneIDi | 1489749 |
KEGGi | vg:1489749 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73559 Genomic RNA No translation available. AF029248 Genomic RNA Translation: AAB86820.1 M27198 Genomic RNA Translation: AAA74011.1 |
PIRi | A32440 S15760 |
RefSeqi | NP_045298.1, NC_001846.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2M0A | NMR | - | A | 833-946 | [»] | |
BMRBi | P0C6V0 | |||||
SMRi | P0C6V0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
GeneIDi | 1489749 |
KEGGi | vg:1489749 |
Enzyme and pathway databases
BRENDAi | 3.4.22.B14, 3442 |
Family and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.40.220.10, 1 hit 3.90.70.90, 1 hit |
InterProi | View protein in InterPro IPR022570, B-CoV_NSP1 IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR032592, NSP3_NAR_bCoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR002705, Pept_C30/C16_B_coronavir IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF11963, DUF3477, 2 hits PF01661, Macro, 1 hit PF01831, Peptidase_C16, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | R1A_CVMA5 | |
Accessioni | P0C6V0Primary (citable) accession number: P0C6V0 Secondary accession number(s): P16342 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | December 2, 2020 | |
This is version 81 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families