UniProtKB - P0C6U2 (R1A_CVH22)
Protein
Replicase polyprotein 1a
Gene
1a
Organism
Human coronavirus 229E (HCoV-229E)
Status
Functioni
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).By similarity
Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1054 | For PL1-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1205 | For PL1-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1701 | For PL2-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1863 | For PL2-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3006 | For 3CL-PRO activity | 1 | |
Active sitei | 3109 | For 3CL-PRO activity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1126 – 1157 | C4-type 1PROSITE-ProRule annotationAdd BLAST | 32 | |
Zinc fingeri | 1780 – 1815 | C4-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 36 | |
Zinc fingeri | 4007 – 4023 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4049 – 4062 | By similarityAdd BLAST | 14 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: InterPro
- RNA binding Source: UniProtKB-KW
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB-EC
- transferase activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- induction by virus of host autophagy Source: UniProtKB-KW
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- suppression by virus of host IRF3 activity Source: UniProtKB-KW
- viral genome replication Source: InterPro
- viral protein processing Source: InterPro
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1aShort name: pp1a Alternative name(s): ORF1a polyprotein Cleaved into the following 11 chains: Alternative name(s): p9 Alternative name(s): p87 Alternative name(s): PL1-PRO/PL2-PRO PLP1/PLP2 Papain-like proteinases 1/2 p195 Alternative name(s): Peptide HD2 Alternative name(s): M-PRO nsp5 p34 Alternative name(s): p5 Alternative name(s): p23 Alternative name(s): p12 Alternative name(s): Growth factor-like peptide Short name: GFL p16 |
Gene namesi | ORF Names:1a |
Organismi | Human coronavirus 229E (HCoV-229E) |
Taxonomic identifieri | 11137 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Alphacoronavirus › Duvinacovirus |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1998 – 2018 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2068 – 2088 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2095 – 2115 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2491 – 2511 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2731 – 2751 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2755 – 2775 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2782 – 2802 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2809 – 2829 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2834 – 2854 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3281 – 3301 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3304 – 3324 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3328 – 3348 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3367 – 3387 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3401 – 3421 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3422 – 3442 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3467 – 3487 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- host cell membrane Source: UniProtKB-SubCell
- host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1048 | K → E: Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1054 | C → A, G or S: Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1099 | G → A: Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1099 | G → P: No effect. 1 Publication | 1 | |
Mutagenesisi | 1102 | G → A or S: No effect. 1 Publication | 1 | |
Mutagenesisi | 1126 | C → D or H: Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1128 | C → A, D or P: Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1154 | C → A, H or D: Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1155 | Missing : Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1157 | C → A, D, H or P: Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1163 | C → A or D: No effect. 1 Publication | 1 | |
Mutagenesisi | 1175 | V → H or P: Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1175 | V → N or T: No effect. 1 Publication | 1 | |
Mutagenesisi | 1203 | C → A: Complete loss of PL1-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 1203 | C → D: No effect. 1 Publication | 1 | |
Mutagenesisi | 1218 | D → A, E, H, K, N or Q: No effect. 1 Publication | 1 | |
Mutagenesisi | 1702 | W → L: Complete loss of PL2-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3006 | H → G, S, T or Y: Complete loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3028 | H → G or T: No loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3029 | N → A, D, E or Q: Increase of 3CL-PRO activity. 2 Publications | 1 | |
Mutagenesisi | 3029 | N → G: No loss of 3CL-PRO activity. 2 Publications | 1 | |
Mutagenesisi | 3029 | N → P: 95% loss of 3CL-PRO activity. 2 Publications | 1 | |
Mutagenesisi | 3074 | E → H: No loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3099 | T → D: Complete loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3109 | C → P, S or V: Complete loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3127 | H → S: Complete loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3136 | H → A: 67% loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3136 | H → S: 77% loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3136 | H → T: 93% loss of 3CL-PRO activity. 1 Publication | 1 | |
Mutagenesisi | 3267 | Q → A: No loss of 3CL-PRO activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000338182 | 1 – 4085 | Replicase polyprotein 1aAdd BLAST | 4085 | |
ChainiPRO_0000338183 | 1 – 111 | Non-structural protein 1Add BLAST | 111 | |
ChainiPRO_0000338184 | 112 – 897 | Non-structural protein 2Add BLAST | 786 | |
ChainiPRO_0000338185 | 898 – 2484 | Non-structural protein 3Add BLAST | 1587 | |
ChainiPRO_0000338186 | 2485 – 2965 | Non-structural protein 4Add BLAST | 481 | |
ChainiPRO_0000338187 | 2966 – 3267 | 3C-like proteinaseAdd BLAST | 302 | |
ChainiPRO_0000338188 | 3268 – 3546 | Non-structural protein 6Add BLAST | 279 | |
ChainiPRO_0000338189 | 3547 – 3629 | Non-structural protein 7Add BLAST | 83 | |
ChainiPRO_0000338190 | 3630 – 3824 | Non-structural protein 8Add BLAST | 195 | |
ChainiPRO_0000338191 | 3825 – 3933 | Non-structural protein 9Add BLAST | 109 | |
ChainiPRO_0000338192 | 3934 – 4068 | Non-structural protein 10Add BLAST | 135 | |
ChainiPRO_0000338193 | 4069 – 4085 | Non-structural protein 11Sequence analysisAdd BLAST | 17 |
Post-translational modificationi
Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 111 – 112 | Cleavage; by PL1-PRO | 2 | |
Sitei | 897 – 898 | Cleavage; by PL1-PRO | 2 | |
Sitei | 2484 – 2485 | Cleavage; by PL2-PRO | 2 | |
Sitei | 2965 – 2966 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3267 – 3268 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3546 – 3547 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3629 – 3630 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3824 – 3825 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 3933 – 3934 | Cleavage; by 3CL-PRO | 2 | |
Sitei | 4068 – 4069 | Cleavage; by 3CL-PRO | 2 |
Proteomic databases
PRIDEi | P0C6U2 |
Interactioni
Subunit structurei
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
By similarityStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P0C6U2 |
SMRi | P0C6U2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0C6U2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1016 – 1268 | Peptidase C16 1PROSITE-ProRule annotationAdd BLAST | 253 | |
Domaini | 1269 – 1436 | MacroPROSITE-ProRule annotationAdd BLAST | 168 | |
Domaini | 1663 – 1914 | Peptidase C16 2PROSITE-ProRule annotationAdd BLAST | 252 | |
Domaini | 2966 – 3267 | Peptidase C30PROSITE-ProRule annotationAdd BLAST | 302 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1925 – 2115 | HD1Add BLAST | 191 | |
Regioni | 2491 – 2854 | HD2Add BLAST | 364 | |
Regioni | 3281 – 3487 | HD3Add BLAST | 207 |
Domaini
The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.
Sequence similaritiesi
Belongs to the coronaviruses polyprotein 1ab family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1126 – 1157 | C4-type 1PROSITE-ProRule annotationAdd BLAST | 32 | |
Zinc fingeri | 1780 – 1815 | C4-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 36 | |
Zinc fingeri | 4007 – 4023 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4049 – 4062 | By similarityAdd BLAST | 14 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.370, 1 hit 2.30.30.1000, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.40.220.10, 1 hit 3.90.70.90, 2 hits |
InterProi | View protein in InterPro IPR038634, A-CoV_nsp1_sf IPR023298, ATPase_P-typ_TM_dom_sf IPR043613, CoV_NSP2_C IPR043615, CoV_NSP2_N IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR011050, Pectin_lyase_fold/virulence IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043503, PLpro_palm_finger_dom_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 2 hits PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 2 hits PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF50494, SSF50494, 1 hit SSF51126, SSF51126, 1 hit SSF52949, SSF52949, 1 hit SSF81665, SSF81665, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform Replicase polyprotein 1a (identifier: P0C6U2-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MACNRVTLAV ASDSEISANG CSTIAQAVRR YSEAASNGFR ACRFVSLDLQ
60 70 80 90 100
DCIVGIADDT YVMGLHGNQT LFCNIMKFSD RPFMLHGWLV FSNSNYLLEE
110 120 130 140 150
FDVVFGKRGG GNVTYTDQYL CGADGKPVMS EDLWQFVDHF GENEEIIING
160 170 180 190 200
HTYVCAWLTK RKPLDYKRQN NLAIEEIEYV HGDALHTLRN GSVLEMAKEV
210 220 230 240 250
KTSSKVVLSD ALDKLYKVFG SPVMTNGSNI LEAFTKPVFI SALVQCTCGT
260 270 280 290 300
KSWSVGDWTG FKSSCCNVIS NKLCVVPGNV KPGDAVITTQ QAGAGIKYFC
310 320 330 340 350
GMTLKFVANI EGVSVWRVIA LQSVDCFVAS STFVEEEHVN RMDTFCFNVR
360 370 380 390 400
NSVTDECRLA MLGAEMTSNV RRQVASGVID ISTGWFDVYD DIFAESKPWF
410 420 430 440 450
VRKAEDIFGP CWSALASALK QLKVTTGELV RFVKSICNSA VAVVGGTIQI
460 470 480 490 500
LASVPEKFLN AFDVFVTAIQ TVFDCAVETC TIAGKAFDKV FDYVLLDNAL
510 520 530 540 550
VKLVTTKLKG VRERGLNKVK YATVVVGSTE EVKSSRVERS TAVLTIANNY
560 570 580 590 600
SKLFDEGYTV VIGDVAYFVS DGYFRLMASP NSVLTTAVYK PLFAFNVNVM
610 620 630 640 650
GTRPEKFPTT VTCENLESAV LFVNDKITEF QLDYSIDVID NEIIVKPNIS
660 670 680 690 700
LCVPLYVRDY VDKWDDFCRQ YSNESWFEDD YRAFISVLDI TDAAVKAAES
710 720 730 740 750
KAFVDTIVPP CPSILKVIDG GKIWNGVIKN VNSVRDWLKS LKLNLTQQGL
760 770 780 790 800
LGTCAKRFKR WLGILLEAYN AFLDTVVSTV KIGGLTFKTY AFDKPYIVIR
810 820 830 840 850
DIVCKVENKT EAEWIELFPH NDRIKSFSTF ESAYMPIADP THFDIEEVEL
860 870 880 890 900
LDAEFVEPGC GGILAVIDEH VFYKKDGVYY PSNGTNILPV AFTKAAGGKV
910 920 930 940 950
SFSDDVEVKD IEPVYRVKLC FEFEDEKLVD VCEKAIGKKI KHEGDWDSFC
960 970 980 990 1000
KTIQSALSVV SCYVNLPTYY IYDEEGGNDL SLPVMISEWP LSVQQAQQEA
1010 1020 1030 1040 1050
TLPDIAEDVV DQVEEVNSIF DIETVDVKHD VSPFEMPFEE LNGLKILKQL
1060 1070 1080 1090 1100
DNNCWVNSVM LQIQLTGILD GDYAMQFFKM GRVAKMIERC YTAEQCIRGA
1110 1120 1130 1140 1150
MGDVGLCMYR LLKDLHTGFM VMDYKCSCTS GRLEESGAVL FCTPTKKAFP
1160 1170 1180 1190 1200
YGTCLNCNAP RMCTIRQLQG TIIFVQQKPE PVNPVSFVVK PVCSSIFRGA
1210 1220 1230 1240 1250
VSCGHYQTNI YSQNLCVDGF GVNKIQPWTN DALNTICIKD ADYNAKVEIS
1260 1270 1280 1290 1300
VTPIKNTVDT TPKEEFVVKE KLNAFLVHDN VAFYQGDVDT VVNGVDFDFI
1310 1320 1330 1340 1350
VNAANENLAH GGGLAKALDV YTKGKLQRLS KEHIGLAGKV KVGTGVMVEC
1360 1370 1380 1390 1400
DSLRIFNVVG PRKGKHERDL LIKAYNTINN EQGTPLTPIL SCGIFGIKLE
1410 1420 1430 1440 1450
TSLEVLLDVC NTKEVKVFVY TDTEVCKVKD FVSGLVNVQK VEQPKIEPKP
1460 1470 1480 1490 1500
VSVIKVAPKP YRVDGKFSYF TEDLLCVADD KPIVLFTDSM LTLDDRGLAL
1510 1520 1530 1540 1550
DNALSGVLSA AIKDCVDINK AIPSGNLIKF DIGSVVVYMC VVPSEKDKHL
1560 1570 1580 1590 1600
DNNVQRCTRK LNRLMCDIVC TIPADYILPL VLSSLTCNVS FVGELKAAEA
1610 1620 1630 1640 1650
KVITIKVTED GVNVHDVTVT TDKSFEQQVG VIADKDKDLS GAVPSDLNTS
1660 1670 1680 1690 1700
ELLTKAIDVD WVEFYGFKDA VTFATVDHSA FAYESAVVNG IRVLKTSDNN
1710 1720 1730 1740 1750
CWVNAVCIAL QYSKPHFISQ GLDAAWNKFV LGDVEIFVAF VYYVARLMKG
1760 1770 1780 1790 1800
DKGDAEDTLT KLSKYLANEA QVQLEHYSSC VECDAKFKNS VASINSAIVC
1810 1820 1830 1840 1850
ASVKRDGVQV GYCVHGIKYY SRVRSVRGRA IIVSVEQLEP CAQSRLLSGV
1860 1870 1880 1890 1900
AYTAFSGPVD KGHYTVYDTA KKSMYDGDRF VKHDLSLLSV TSVVMVGGYV
1910 1920 1930 1940 1950
APVNTVKPKP VINQLDEKAQ KFFDFGDFLI HNFVIFFTWL LSMFTLCKTA
1960 1970 1980 1990 2000
VTTGDVKIMA KAPQRTGVVL KRSLKYNLKA SAAVLKSKWW LLAKFTKLLL
2010 2020 2030 2040 2050
LIYTLYSVVL LCVRFGPFNF CSETVNGYAK SNFVKDDYCD GSLGCKMCLF
2060 2070 2080 2090 2100
GYQELSQFSH LDVVWKHITD PLFSNMQPFI VMVLLLIFGD NYLRCFLLYF
2110 2120 2130 2140 2150
VAQMISTVGV FLGYKETNWF LHFIPFDVIC DELLVTVIVI KVISFVRHVL
2160 2170 2180 2190 2200
FGCENPDCIA CSKSARLKRF PVNTIVNGVQ RSFYVNANGG SKFCKKHRFF
2210 2220 2230 2240 2250
CVDCDSYGYG STFITPEVSR ELGNITKTNV QPTGPAYVMI DKVEFENGFY
2260 2270 2280 2290 2300
RLYSCETFWR YNFDITESKY SCKEVFKNCN VLDDFIVFNN NGTNVTQVKN
2310 2320 2330 2340 2350
ASVYFSQLLC RPIKLVDSEL LSTLSVDFNG VLHKAYIDVL RNSFGKDLNA
2360 2370 2380 2390 2400
NMSLAECKRA LGLSISDHEF TSAISNAHRC DVLLSDLSFN NFVSSYAKPE
2410 2420 2430 2440 2450
EKLSAYDLAC CMRAGAKVVN ANVLTKDQTP IVWHAKDFNS LSAEGRKYIV
2460 2470 2480 2490 2500
KTSKAKGLTF LLTINENQAV TQIPATSIVA KQGAGDAGHS LTWLWLLCGL
2510 2520 2530 2540 2550
VCLIQFYLCF FMPYFMYDIV SSFEGYDFKY IENGQLKNFE APLKCVRNVF
2560 2570 2580 2590 2600
ENFEDWHYAK FGFTPLNKQS CPIVVGVSEI VNTVAGIPSN VYLVGKTLIF
2610 2620 2630 2640 2650
TLQAAFGNAG VCYDIFGVTT PEKCIFTSAC TRLEGLGGNN VYCYNTALME
2660 2670 2680 2690 2700
GSLPYSSIQA NAYYKYDNGN FIKLPEVIAQ GFGFRTVRTI ATKYCRVGEC
2710 2720 2730 2740 2750
VESNAGVCFG FDKWFVNDGR VANGYVCGTG LWNLVFNILS MFSSSFSVAA
2760 2770 2780 2790 2800
MSGQILLNCA LGAFAIFCCF LVTKFRRMFG DLSVGVCTVV VAVLLNNVSY
2810 2820 2830 2840 2850
IVTQNLVTMI AYAILYFFAT RSLRYAWIWC AAYLIAYISF APWWLCAWYF
2860 2870 2880 2890 2900
LAMLTGLLPS LLKLKVSTNL FEGDKFVGTF ESAAAGTFVI DMRSYEKLAN
2910 2920 2930 2940 2950
SISPEKLKSY AASYNRYKYY SGNANEADYR CACYAYLAKA MLDFSRDHND
2960 2970 2980 2990 3000
ILYTPPTVSY GSTLQAGLRK MAQPSGFVEK CVVRVCYGNT VLNGLWLGDI
3010 3020 3030 3040 3050
VYCPRHVIAS NTTSAIDYDH EYSIMRLHNF SIISGTAFLG VVGATMHGVT
3060 3070 3080 3090 3100
LKIKVSQTNM HTPRHSFRTL KSGEGFNILA CYDGCAQGVF GVNMRTNWTI
3110 3120 3130 3140 3150
RGSFINGACG SPGYNLKNGE VEFVYMHQIE LGSGSHVGSS FDGVMYGGFE
3160 3170 3180 3190 3200
DQPNLQVESA NQMLTVNVVA FLYAAILNGC TWWLKGEKLF VEHYNEWAQA
3210 3220 3230 3240 3250
NGFTAMNGED AFSILAAKTG VCVERLLHAI QVLNNGFGGK QILGYSSLND
3260 3270 3280 3290 3300
EFSINEVVKQ MFGVNLQSGK TTSMFKSISL FAGFFVMFWA ELFVYTTTIW
3310 3320 3330 3340 3350
VNPGFLTPFM ILLVALSLCL TFVVKHKVLF LQVFLLPSII VAAIQNCAWD
3360 3370 3380 3390 3400
YHVTKVLAEK FDYNVSVMQM DIQGFVNIFI CLFVALLHTW RFAKERCTHW
3410 3420 3430 3440 3450
CTYLFSLIAV LYTALYSYDY VSLLVMLLCA ISNEWYIGAI IFRICRFGVA
3460 3470 3480 3490 3500
FLPVEYVSYF DGVKTVLLFY MLLGFVSCMY YGLLYWINRF CKCTLGVYDF
3510 3520 3530 3540 3550
CVSPAEFKYM VANGLNAPNG PFDALFLSFK LMGIGGPRTI KVSTVQSKLT
3560 3570 3580 3590 3600
DLKCTNVVLM GILSNMNIAS NSKEWAYCVE MHNKINLCDD PETAQELLLA
3610 3620 3630 3640 3650
LLAFFLSKHS DFGLGDLVDS YFENDSILQS VASSFVGMPS FVAYETARQE
3660 3670 3680 3690 3700
YENAVANGSS PQIIKQLKKA MNVAKAEFDR ESSVQKKINR MAEQAAAAMY
3710 3720 3730 3740 3750
KEARAVNRKS KVVSAMHSLL FGMLRRLDMS SVDTILNMAR NGVVPLSVIP
3760 3770 3780 3790 3800
ATSAARLVVV VPDHDSFVKM MVDGFVHYAG VVWTLQEVKD NDGKNVHLKD
3810 3820 3830 3840 3850
VTKENQEILV WPLILTCERV VKLQNNEIMP GKMKVKATKG EGDGGITSEG
3860 3870 3880 3890 3900
NALYNNEGGR AFMYAYVTTK PGMKYVKWEH DSGVVTVELE PPCRFVIDTP
3910 3920 3930 3940 3950
TGPQIKYLYF VKNLNNLRRG AVLGYIGATV RLQAGKQTEF VSNSHLLTHC
3960 3970 3980 3990 4000
SFAVDPAAAY LDAVKQGAKP VGNCVKMLTN GSGSGQAITC TIDSNTTQDT
4010 4020 4030 4040 4050
YGGASVCIYC RAHVAHPTMD GFCQYKGKWV QVPIGTNDPI RFCLENTVCK
4060 4070 4080
VCGCWLNHGC TCDRTAIQSF DNSYLNESGA LVPLD
Note: Produced by conventional translation.
Isoform Replicase polyprotein 1ab (identifier: P0C6X1-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6X1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1982 | A → Q (PubMed:11369870).Curated | 1 | |
Sequence conflicti | 4042 | F → S (PubMed:11369870).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF304460 Genomic RNA Translation: AAG48590.1 X69721 Genomic RNA Translation: CAA49377.1 |
PIRi | S28600 |
RefSeqi | NP_073550.1, NC_002645.1 [P0C6U2-1] |
Genome annotation databases
GeneIDi | 918764 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Proteic grace - Issue 77 of December 2006 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF304460 Genomic RNA Translation: AAG48590.1 X69721 Genomic RNA Translation: CAA49377.1 |
PIRi | S28600 |
RefSeqi | NP_073550.1, NC_002645.1 [P0C6U2-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1P9S | X-ray | 2.54 | A/B | 2966-3265 | [»] | |
2ZU2 | X-ray | 1.80 | A/B | 2966-3267 | [»] | |
3EWQ | X-ray | 2.10 | A | 1269-1436 | [»] | |
3EWR | X-ray | 2.01 | A | 1269-1436 | [»] | |
SASBDBi | P0C6U2 | |||||
SMRi | P0C6U2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PRIDEi | P0C6U2 |
Genome annotation databases
GeneIDi | 918764 |
Miscellaneous databases
EvolutionaryTracei | P0C6U2 |
Family and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.370, 1 hit 2.30.30.1000, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.40.220.10, 1 hit 3.90.70.90, 2 hits |
InterProi | View protein in InterPro IPR038634, A-CoV_nsp1_sf IPR023298, ATPase_P-typ_TM_dom_sf IPR043613, CoV_NSP2_C IPR043615, CoV_NSP2_N IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR002589, Macro_dom IPR043472, Macro_dom-like IPR036333, NSP10_sf_CoV IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR011050, Pectin_lyase_fold/virulence IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043503, PLpro_palm_finger_dom_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 2 hits PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 2 hits PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF50494, SSF50494, 1 hit SSF51126, SSF51126, 1 hit SSF52949, SSF52949, 1 hit SSF81665, SSF81665, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | R1A_CVH22 | |
Accessioni | P0C6U2Primary (citable) accession number: P0C6U2 Secondary accession number(s): Q05002 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | December 2, 2020 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries