UniProtKB - P0C6U0 (R1A_CVBM)
Replicase polyprotein 1a
1a
Functioni
The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).
By similarityResponsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
PROSITE-ProRule annotationNsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
By similarityNsp9 is a ssRNA-binding protein.
By similarityBinds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).
By similarityCatalytic activityi
- TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. EC:3.4.22.69
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1074 | For PL1-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1225 | For PL1-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1671 | For PL2-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1828 | For PL2-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3287 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3391 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Metal bindingi | 4306 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4309 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4315 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4322 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4348 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4351 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4359 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4361 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1151 – 1179 | C4-type 1PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 1749 – 1785 | C4-type 2PROSITE-ProRule annotationAdd BLAST | 37 | |
Zinc fingeri | 4306 – 4322 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4348 – 4361 | By similarityAdd BLAST | 14 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: InterPro
- single-stranded RNA binding Source: InterPro
- thiol-dependent deubiquitinase Source: UniProtKB-EC
- zinc ion binding Source: InterPro
GO - Biological processi
- induction by virus of catabolism of host mRNA Source: UniProtKB-KW
- induction by virus of host autophagy Source: UniProtKB-KW
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- suppression by virus of host gene expression Source: UniProtKB-KW
- suppression by virus of host ISG15-protein conjugation Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
- suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity Source: UniProtKB-KW
- viral genome replication Source: InterPro
- viral protein processing Source: InterPro
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1aShort name: pp1a Alternative name(s): ORF1a polyprotein Cleaved into the following 11 chains: Alternative name(s): p28 Alternative name(s): p65 Alternative name(s): PL1-PRO/PL2-PRO PL1/PL2 Papain-like proteinases 1/2 p210 Alternative name(s): Peptide HD2 p44 Alternative name(s): M-PRO nsp5 p27 Alternative name(s): p10 Alternative name(s): p22 Alternative name(s): p12 Alternative name(s): Growth factor-like peptide Short name: GFL p15 |
Gene namesi | ORF Names:1a |
Organismi | Bovine coronavirus (strain Mebus) (BCoV) (BCV) |
Taxonomic identifieri | 11132 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Embecovirus › |
Virus hosti | Bos taurus (Bovine) [TaxID: 9913] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 2138 – 2158 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2199 – 2219 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2221 – 2241 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2313 – 2333 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2343 – 2363 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2365 – 2385 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2752 – 2772 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3031 – 3051 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3063 – 3083 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3090 – 3110 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3115 – 3135 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3558 – 3578 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3588 – 3608 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3615 – 3635 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3657 – 3677 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3684 – 3704 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3711 – 3731 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3755 – 3775 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000338158 | 1 – 4383 | Replicase polyprotein 1aAdd BLAST | 4383 | |
ChainiPRO_0000338159 | 1 – 246 | Non-structural protein 1By similarityAdd BLAST | 246 | |
ChainiPRO_0000338160 | 247 – 851 | Non-structural protein 2By similarityAdd BLAST | 605 | |
ChainiPRO_0000338161 | 852 – 2750 | Non-structural protein 3By similarityAdd BLAST | 1899 | |
ChainiPRO_0000338162 | 2751 – 3246 | Non-structural protein 4By similarityAdd BLAST | 496 | |
ChainiPRO_0000338163 | 3247 – 3549 | 3C-like proteinaseBy similarityAdd BLAST | 303 | |
ChainiPRO_0000338164 | 3550 – 3836 | Non-structural protein 6By similarityAdd BLAST | 287 | |
ChainiPRO_0000338165 | 3837 – 3925 | Non-structural protein 7By similarityAdd BLAST | 89 | |
ChainiPRO_0000338166 | 3926 – 4122 | Non-structural protein 8By similarityAdd BLAST | 197 | |
ChainiPRO_0000338167 | 4123 – 4232 | Non-structural protein 9By similarityAdd BLAST | 110 | |
ChainiPRO_0000338168 | 4233 – 4369 | Non-structural protein 10By similarityAdd BLAST | 137 | |
ChainiPRO_0000338169 | 4370 – 4383 | Non-structural protein 11Sequence analysisAdd BLAST | 14 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 246 – 247 | Cleavage; by PL1-PROBy similarity | 2 | |
Sitei | 851 – 852 | Cleavage; by PL1-PROBy similarity | 2 | |
Sitei | 2750 – 2751 | Cleavage; by PL2-PROBy similarity | 2 | |
Sitei | 3246 – 3247 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3549 – 3550 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3836 – 3837 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3925 – 3926 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 4122 – 4123 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 4232 – 4233 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 4369 – 4370 | Cleavage; by 3CL-PROBy similarity | 2 |
Interactioni
Subunit structurei
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
By similarityProtein-protein interaction databases
IntActi | P0C6U0, 1 interactor |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 54 – 197 | CoV Nsp1 globularPROSITE-ProRule annotationAdd BLAST | 144 | |
Domaini | 216 – 246 | BetaCoV Nsp1 C-terminalPROSITE-ProRule annotationAdd BLAST | 31 | |
Domaini | 852 – 966 | Ubiquitin-like 1PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 1036 – 1274 | Peptidase C16 1PROSITE-ProRule annotationAdd BLAST | 239 | |
Domaini | 1275 – 1435 | MacroPROSITE-ProRule annotationAdd BLAST | 161 | |
Domaini | 1491 – 1563 | DPUPPROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 1562 – 1617 | Ubiquitin-like 2PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 1631 – 1892 | Peptidase C16 2PROSITE-ProRule annotationAdd BLAST | 262 | |
Domaini | 1906 – 2007 | Nucleic acid-bindingPROSITE-ProRule annotationAdd BLAST | 102 | |
Domaini | 3149 – 3246 | Nsp4CPROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 3247 – 3549 | Peptidase C30PROSITE-ProRule annotationAdd BLAST | 303 | |
Domaini | 3837 – 3925 | RdRp Nsp7 cofactorPROSITE-ProRule annotationAdd BLAST | 89 | |
Domaini | 3926 – 4122 | RdRp Nsp8 cofactorPROSITE-ProRule annotationAdd BLAST | 197 | |
Domaini | 4123 – 4232 | Nsp9 ssRNA-bindingPROSITE-ProRule annotationAdd BLAST | 110 | |
Domaini | 4233 – 4370 | ExoN/MTase coactivatorPROSITE-ProRule annotationAdd BLAST | 138 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 972 – 992 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 2138 – 2385 | HD1Add BLAST | 248 | |
Regioni | 2752 – 3135 | HD2Add BLAST | 384 | |
Regioni | 3558 – 3775 | HD3Add BLAST | 218 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1151 – 1179 | C4-type 1PROSITE-ProRule annotationAdd BLAST | 29 | |
Zinc fingeri | 1749 – 1785 | C4-type 2PROSITE-ProRule annotationAdd BLAST | 37 | |
Zinc fingeri | 4306 – 4322 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4348 – 4361 | By similarityAdd BLAST | 14 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
CDDi | cd21560, betaCoV-Nsp6, 1 hit cd21519, cv_beta_Nsp2_MHV-like, 1 hit cd21473, cv_Nsp4_TM, 1 hit cd21524, DPUP_MHV_Nsp3, 1 hit cd21557, Macro_X_Nsp3-like, 1 hit cd21467, Ubl1_cv_Nsp3_N-like, 1 hit cd21466, Ubl2_cv_PLpro_N_Nsp3-like, 1 hit |
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 2 hits 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.60.120.1680, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.30.70.3540, 1 hit 3.40.220.10, 1 hit 3.40.50.11020, 1 hit |
InterProi | View protein in InterPro IPR022570, B-CoV_A_NSP1 IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR022733, DPUP_SUD_C_bCoV IPR002589, Macro_dom IPR043472, Macro_dom-like IPR044371, Macro_X_NSP3-like IPR042570, NAR_sf IPR036333, NSP10_sf_CoV IPR044384, NSP2_MHV-like IPR044381, NSP3_DPUP_MHV IPR032592, NSP3_NAB_bCoV IPR044357, NSP3_Ubl1_dom_CoV IPR044353, Nsp3_Ubl2_dom_CoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR044367, NSP6_betaCoV IPR043610, NSP6_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR002705, Pept_C30/C16_B_coronavir IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF11963, DUF3477, 1 hit PF01661, Macro, 1 hit PF01831, Peptidase_C16, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51963, BCOV_NSP1_C, 1 hit PS51942, BCOV_NSP3C_C, 1 hit PS51945, BCOV_NSP3E_NAB, 1 hit PS51952, COV_EXON_MTASE_COACT, 1 hit PS51962, COV_NSP1, 1 hit PS51943, COV_NSP3A_UBL, 1 hit PS51944, COV_NSP3D_UBL, 1 hit PS51946, COV_NSP4C, 1 hit PS51949, COV_NSP7, 1 hit PS51950, COV_NSP8, 1 hit PS51951, COV_NSP9_SSRNA_BD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSKINKYGLE LHWAPEFPWM FEDAEEKLDN PSSSEVDIVC STTAQKLETG
60 70 80 90 100
GICPENHVMV DCRRLLKQEC CVQSSLIREI VMNTRPYDLE VLLQDALQSR
110 120 130 140 150
EAVLVTPPLG MSLEACYVRG CNPNGWTMGL FRRRSVCNTG RCAVNKHVAY
160 170 180 190 200
QLYMIDPAGV CFGAGQFVGW VIPLAFMPVQ SRKFIVPWVM YLRKCGEKGA
210 220 230 240 250
YNKDHKRGGF EHVYNFKVED AYDLVHDEPK GKFSKKAYAL IRGYRGVKPL
260 270 280 290 300
LYVDQYGCDY TGGLADGLEA YADKTLQEMK ALFPIWSQEL PFDVTVAWHV
310 320 330 340 350
VRDPRYVMRL QSASTIRSVA YVANPTEDLC DGSVVIKEPV HVYADDSIIL
360 370 380 390 400
RQHNLVDIMS CFYMEADAVV NAFYGVDLKD CGFVMQFGYI DCEQDLCDFK
410 420 430 440 450
GWVPGNMIDG FACTTCGHVY ETGDLLAQSS GVLPVNPVLH TKSAAGYGGF
460 470 480 490 500
GCKDSFTLYG QTVVYFGGCV YWSPARNIWI PILKSSVKSY DGLVYTGVVG
510 520 530 540 550
CKAIVKETNL ICKALYLDYV QHKCGNLHQR ELLGVSDVWH KQLLLNRGVY
560 570 580 590 600
KPLLENIDYF NMRRAKFSLE TFTVCADGFM PFLLDDLVPR AYYLAVSGQA
610 620 630 640 650
FCDYAGKICH AVVSKSKELL DVSLDSLGAA IHYLNSKIVD LAQHFSDFGT
660 670 680 690 700
SFVSKIVHFF KTFTTSTALA FAWVLFHVLH GAYIVVESDI YFVKNIPRYA
710 720 730 740 750
SAVAQAFRSV AKVVLDSLRV TFIDGLSCFK IGRRRICLSG SKIYEVERGL
760 770 780 790 800
LHSSQLPLDV YDLTMPSQVQ KTKQKPIYLK GSGSDFSLAD SVVEVVTTSL
810 820 830 840 850
TPCGYSEPPK VADKICIVDN VYMAKAGDKY YPVVVDGHVG LLDQAWRVPC
860 870 880 890 900
AGRCVTFKEQ PTVNEIASTP KTIKVFYELD KDFNTILNTA CGVFEVDDTV
910 920 930 940 950
DMEEFYAVVI DAIEEKLSPC KELEGVGAKV SAFLQKLEDN SLFLFDEAGE
960 970 980 990 1000
EVLAPKLYCA FTAPEDDDFL EESGVEEDDV EGEETDLTVT SAGEPCVASE
1010 1020 1030 1040 1050
QEESSEILED TLDDGPCVET SDSQVEEDVQ MSDFVDLESV IQDYENVCFE
1060 1070 1080 1090 1100
FYTTEPEFVK VLDLYVPKAT RNNCWLRSVL AVMQKLPCQF KDKNLQDLWV
1110 1120 1130 1140 1150
LYKQQYSQLF VDTLVNKIPA NIVVPQGGYV ADFAYWFLTL CDWQCVAYWK
1160 1170 1180 1190 1200
CIKCDLALKL KGLDAMFFYG DVVSHVCKCG ESMVLIDVDV PFTAHFALKD
1210 1220 1230 1240 1250
KLFCAFITKR SVYKAACVVD VNDSHSMAVV DGKQIDDHRI TSITSDKFDF
1260 1270 1280 1290 1300
IIGHGMSFSM TTFEIAQLYG SCITPNVCFV KGDIIKVSKR VKAEVVVNPA
1310 1320 1330 1340 1350
NGHMAHGGGV AKAIAVAAGQ QFVKETTDMV KSKGVCATGD CYVSTGGKLC
1360 1370 1380 1390 1400
KTVLNVVGPD ARTQGKQSYA LLERVYKHLN KYDCVVTTLI SAGIFSVPSD
1410 1420 1430 1440 1450
VSLTYLLGTA KKQVVLVSNN QEDFDLISKC QITAVEGTKK LAERLSFNVG
1460 1470 1480 1490 1500
RSIVYETDAN KLILSNDVAF VSTFNVLQDV LSLRHDIALD DDARTFVQSN
1510 1520 1530 1540 1550
VDVVPEGWRV VNKFYQINGV RTVKYFECPG GIDICSQDKV FGYVQQGSFN
1560 1570 1580 1590 1600
KATVAQIKAL FLDKVDILLT VDGVNFTNRF VPVGESFGKS LGNVFCDGVN
1610 1620 1630 1640 1650
VTKHKCDINY KGKVFFQFDN LSSEDLKAVR SSFNFDQKEL LAYYNMLVNC
1660 1670 1680 1690 1700
SKWQVVFNGK YFTFKQANNN CFVNVSCLML QSLNLKFKIV QWQEAWLEFR
1710 1720 1730 1740 1750
SGRPARFVSL VLAKGGFKFG DPADSRDFLR VVFSQVDLTG AICDFEIACK
1760 1770 1780 1790 1800
CGVKQEQRTG VDAVMHFGTL SREDLEIGYT VDCSCGKKLI HCVRFDVPFL
1810 1820 1830 1840 1850
ICSNTPASVK LPKGVGSANI FKGDKVGHYV HVKCEQSYQL YDASNVKKVT
1860 1870 1880 1890 1900
DVTGNLSDCL YLKNLKQTFK SVLTTYYLDD VKKIEYKPDL SQYYCDGGKY
1910 1920 1930 1940 1950
YTQRIIKAQF KTFEKVDGVY TNFKLIGHTV CDILNAKLGF DSSKEFVEYK
1960 1970 1980 1990 2000
VTEWPTATGD VVLATDDLYV KRYERGCITF GKPVIWLSHE QASLNSLTYF
2010 2020 2030 2040 2050
NRPLLVDENK FDVLKVDDVD DGGDISESDA KEPKEINIIK LSGVKKPFKV
2060 2070 2080 2090 2100
EDSVIVNDDT SEIKYVKSLS IVDVYDMWLT GCRCVVRTAN ALSRAVNVPT
2110 2120 2130 2140 2150
IRKFIKFGMT LVSIPIDLLN LREIKPVFNV VKAVRNKISA CFNFIKWLFV
2160 2170 2180 2190 2200
LLFGWIKISA DNKVIYTTEV ASKLTCKLVA LAFKNAFLTF KWSVVARGAC
2210 2220 2230 2240 2250
IIATIFLLWF NFIYANVIFS DFYLPKIGFL PTFVGKIAQW IKNTFSLVTI
2260 2270 2280 2290 2300
CDLYSIQDVG FKNQYCNGSI ACQFCLAGFD MLDNYKAIDV VQYEADRRAF
2310 2320 2330 2340 2350
VDYTGVLKIV IELIVSYALY TAWFYPLFAL ISIQILTTWL PELFMLSTLH
2360 2370 2380 2390 2400
WSVRLLVSLA NMLPAHVFMR FYIIIASFIK LFSLFRHVAY GCSKSGCLFC
2410 2420 2430 2440 2450
YKRNRSLRVK CSTIVGGMIR YYDAMANGGT GFCSKHQWNC IDCDSYKPGN
2460 2470 2480 2490 2500
TFITVEAALD LSKELKRPIQ PTDVAYHTVT DVKQVGCYMR LFYDRDGQRT
2510 2520 2530 2540 2550
YDDVNASLFV DYSNLLHSKV KSVPNMHVVV VENDADKANF LNAAVFYAQS
2560 2570 2580 2590 2600
LFRPILMVDK NLITTANTGT SVTETMFDVY VDTFLSMFDV DKKSLNALIA
2610 2620 2630 2640 2650
TAHSSIKQGT QICKVLDTFL SCARKSCSID SDVDTKCLAD SVMSAVSAGL
2660 2670 2680 2690 2700
ELTDESCNNL VPTYLKGDNI VAADLGVLIQ NSAKHVQGNV AKIAGVSCIW
2710 2720 2730 2740 2750
SVDAFNQLSS DFQHKLKKAC CKTGLKLKLT YNKQMANVSV LTTPFSLKGG
2760 2770 2780 2790 2800
AVFSYFVYVC FVLSLVCFIG LWCLMPTYTV HKSDFQLPVY ASYKVLDNGV
2810 2820 2830 2840 2850
IRDVSVEDVC FANKFEQFDQ WYESTFGLSY YSNSMACPIV VAVVDQDFGS
2860 2870 2880 2890 2900
TVFNVPTKVL RYGYHVLHFI THALSADGVQ CYTPHSQISY SNFYASGCVL
2910 2920 2930 2940 2950
SSACTMFAMA DGSPQPYCYT DGLMQNASLY SSLVPHVRYN LANAKGFIRF
2960 2970 2980 2990 3000
PEVLREGLVR IVRTRSMPYC RVGLCEEADE GICFNFNGSW VLNNDYYRSL
3010 3020 3030 3040 3050
PGTFCGRDVF DLIYQLFKGL AQPVDFLALT ASSIAGAILA VIVVLVFYYL
3060 3070 3080 3090 3100
IKLKRAFGDY TSIVFVNVIV WCVNFMMLFV FQVYPTLSCV YAICYFYATL
3110 3120 3130 3140 3150
YFPSEISVIM HLQWLVMYGT IMPLWFCLLY ISVVVSNHAF WVFSYCRQLG
3160 3170 3180 3190 3200
TSVRSDGTFE EMALTTFMIT KDSYCKLKNS LSDVAFNRYL SLYNKYRYYS
3210 3220 3230 3240 3250
GKMDTAAYRE AACSQLAKAM DTFTNNNGSD VLYQPPTASV STSFLQSGIV
3260 3270 3280 3290 3300
KMVNPTSKVE PCIVSVTYGN MTLNGLWLGD KVYCPRHVIC SASDMTNPDY
3310 3320 3330 3340 3350
TNLLCRVTSS DFTVLFDRLS LTVMSYQMQG CMLVLTVTLQ NSRTPKYTFG
3360 3370 3380 3390 3400
VVKPGETFTV LAAYNGKPQG AFHVTMRSSY TIKGSFLCGS CGSVVYVIMG
3410 3420 3430 3440 3450
DCVKFVYMHQ LELSTGCHTG TDFNGDFYGP YKDAQVVQLP VQDYIQSVNF
3460 3470 3480 3490 3500
VAWLYAAILN NCNWFVQSDK CSVEDFNVWA LSNGFSQVKS DLVIDALASM
3510 3520 3530 3540 3550
TGVSLETLLA AIKHLKNGFQ GRQIMGSCSF EDELTPSDVY QQLAGIKLQS
3560 3570 3580 3590 3600
KRTRLVKGIV CWIMASTFLF SCIITAFVKW TMFMYVTTNM LSITFCALCV
3610 3620 3630 3640 3650
ISLTMLLVKH KHLYLTMYII PVLFTLLYNN YLVVYKQTFR GYVYAWLSYY
3660 3670 3680 3690 3700
VPSVEYTYTD EVIYGMLLLI GMVFVTLRSI NQYLFSFIMF VGRVISVVSL
3710 3720 3730 3740 3750
WYMGSNLEEE ILLMLASLFG TYTWTTALSM AAAKVIAKWV AVNVLYFTDI
3760 3770 3780 3790 3800
PQIKIVLVCY LFIGYIISCY WGLFSLMNSL FRMPLGVYNY KISVQELRYM
3810 3820 3830 3840 3850
NANGLRPPKN SFEALMLNFK LLGIGGVPII EVSQFQSKLT DVKCANVVLL
3860 3870 3880 3890 3900
NCLQHLHVAS NSKLWQYCST LHNEILATSD LGVAFEKLAQ LLIVLFANPA
3910 3920 3930 3940 3950
AVDSKCLTSI EEVCDDYAKD NTVLQALQSE FVNMASFVEY EVAKKNLDEA
3960 3970 3980 3990 4000
CSSGSANQQQ LKQLEKACNI AKSAYERDRA VARKLERMAD LALTNMYKEA
4010 4020 4030 4040 4050
RINDKKSKVV SALQTMLFSM VRKLDNQALN SILDNAVKGC VPLNAIPSLA
4060 4070 4080 4090 4100
ANTLTIIVPD KSVYDQVVDN VYVTYAGNVW QIQTIQDSDG TNKQLHEISD
4110 4120 4130 4140 4150
DCNWPLVIIA NRHNEVSATA LQNNELMPAK LKTQVVNSGP DQTCNTPTQC
4160 4170 4180 4190 4200
YYNNSYNGKI VYAILSDVDG LKYTKILKDD GNFVVLELDP PCKFTVQDVK
4210 4220 4230 4240 4250
GLKIKYLYFV KGCNTLARGW VVGTISSTVR LQAGTATEYA SNSSILSLCA
4260 4270 4280 4290 4300
FSVDPKKTYL DFIQQGGTPI ANCVKMLCDH AGTGMAITVK PDATTSQDSY
4310 4320 4330 4340 4350
GGASVCIYCR ARVEHPDVDG LCKLRGKFVQ VPVGIKDPVS YVLTHDVCQV
4360 4370 4380
CGFWRDGSCS CVSTDTTVQS KDTNFLNGFG VRV
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00735 Genomic RNA No translation available. |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00735 Genomic RNA No translation available. |
3D structure databases
SMRi | P0C6U0 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P0C6U0, 1 interactor |
Family and domain databases
CDDi | cd21560, betaCoV-Nsp6, 1 hit cd21519, cv_beta_Nsp2_MHV-like, 1 hit cd21473, cv_Nsp4_TM, 1 hit cd21524, DPUP_MHV_Nsp3, 1 hit cd21557, Macro_X_Nsp3-like, 1 hit cd21467, Ubl1_cv_Nsp3_N-like, 1 hit cd21466, Ubl2_cv_PLpro_N_Nsp3-like, 1 hit |
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 2 hits 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.60.120.1680, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.30.70.3540, 1 hit 3.40.220.10, 1 hit 3.40.50.11020, 1 hit |
InterProi | View protein in InterPro IPR022570, B-CoV_A_NSP1 IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR022733, DPUP_SUD_C_bCoV IPR002589, Macro_dom IPR043472, Macro_dom-like IPR044371, Macro_X_NSP3-like IPR042570, NAR_sf IPR036333, NSP10_sf_CoV IPR044384, NSP2_MHV-like IPR044381, NSP3_DPUP_MHV IPR032592, NSP3_NAB_bCoV IPR044357, NSP3_Ubl1_dom_CoV IPR044353, Nsp3_Ubl2_dom_CoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR044367, NSP6_betaCoV IPR043610, NSP6_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR002705, Pept_C30/C16_B_coronavir IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF11963, DUF3477, 1 hit PF01661, Macro, 1 hit PF01831, Peptidase_C16, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51963, BCOV_NSP1_C, 1 hit PS51942, BCOV_NSP3C_C, 1 hit PS51945, BCOV_NSP3E_NAB, 1 hit PS51952, COV_EXON_MTASE_COACT, 1 hit PS51962, COV_NSP1, 1 hit PS51943, COV_NSP3A_UBL, 1 hit PS51944, COV_NSP3D_UBL, 1 hit PS51946, COV_NSP4C, 1 hit PS51949, COV_NSP7, 1 hit PS51950, COV_NSP8, 1 hit PS51951, COV_NSP9_SSRNA_BD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | R1A_CVBM | |
Accessioni | P0C6U0Primary (citable) accession number: P0C6U0 Secondary accession number(s): Q66198 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | February 23, 2022 | |
This is version 79 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |