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UniProtKB - P0C6U0 (R1A_CVBM)

Entry version 74 (02 Dec 2020)
Sequence version 1 (10 Jun 2008)
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Protein

Replicase polyprotein 1a

Gene

1a

Organism
Bovine coronavirus (strain Mebus) (BCoV) (BCV)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. EC:3.4.22.69
  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei1074For PL1-PRO activityPROSITE-ProRule annotation1
Active sitei1225For PL1-PRO activityPROSITE-ProRule annotation1
Active sitei1671For PL2-PRO activityPROSITE-ProRule annotation1
Active sitei1828For PL2-PRO activityPROSITE-ProRule annotation1
Active sitei3287For 3CL-PRO activityPROSITE-ProRule annotation1
Active sitei3391For 3CL-PRO activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri1151 – 1179C4-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri1749 – 1785C4-type 2PROSITE-ProRule annotationAdd BLAST37
Zinc fingeri4306 – 4322By similarityAdd BLAST17
Zinc fingeri4348 – 4361By similarityAdd BLAST14

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, RNA-binding, Thiol protease
Biological processActivation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion
LigandMetal-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
p28
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65
Non-structural protein 3 (EC:3.4.19.12, EC:3.4.22.69)
Short name:
nsp3
Alternative name(s):
PL1-PRO/PL2-PRO
PL1/PL2
Papain-like proteinases 1/2
p210
Non-structural protein 4
Short name:
nsp4
Alternative name(s):
Peptide HD2
p44
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
M-PRO
nsp5
p27
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Alternative name(s):
p10
Non-structural protein 8
Short name:
nsp8
Alternative name(s):
p22
Non-structural protein 9
Short name:
nsp9
Alternative name(s):
p12
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
p15
Non-structural protein 11
Short name:
nsp11
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
ORF Names:1a
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBovine coronavirus (strain Mebus) (BCoV) (BCV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri11132 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiViruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Embecovirus ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiBos taurus (Bovine) [TaxID: 9913]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000007554 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

  • host perinuclear region By similarity
    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
  • host perinuclear region By similarity
    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
  • host perinuclear region By similarity
    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
  • host perinuclear region By similarity
    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei2138 – 2158HelicalSequence analysisAdd BLAST21
Transmembranei2199 – 2219HelicalSequence analysisAdd BLAST21
Transmembranei2221 – 2241HelicalSequence analysisAdd BLAST21
Transmembranei2313 – 2333HelicalSequence analysisAdd BLAST21
Transmembranei2343 – 2363HelicalSequence analysisAdd BLAST21
Transmembranei2365 – 2385HelicalSequence analysisAdd BLAST21
Transmembranei2752 – 2772HelicalSequence analysisAdd BLAST21
Transmembranei3031 – 3051HelicalSequence analysisAdd BLAST21
Transmembranei3063 – 3083HelicalSequence analysisAdd BLAST21
Transmembranei3090 – 3110HelicalSequence analysisAdd BLAST21
Transmembranei3115 – 3135HelicalSequence analysisAdd BLAST21
Transmembranei3558 – 3578HelicalSequence analysisAdd BLAST21
Transmembranei3588 – 3608HelicalSequence analysisAdd BLAST21
Transmembranei3615 – 3635HelicalSequence analysisAdd BLAST21
Transmembranei3657 – 3677HelicalSequence analysisAdd BLAST21
Transmembranei3684 – 3704HelicalSequence analysisAdd BLAST21
Transmembranei3711 – 3731HelicalSequence analysisAdd BLAST21
Transmembranei3755 – 3775HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003381581 – 4383Replicase polyprotein 1aAdd BLAST4383
ChainiPRO_00003381591 – 246Non-structural protein 1By similarityAdd BLAST246
ChainiPRO_0000338160247 – 851Non-structural protein 2By similarityAdd BLAST605
ChainiPRO_0000338161852 – 2750Non-structural protein 3By similarityAdd BLAST1899
ChainiPRO_00003381622751 – 3246Non-structural protein 4By similarityAdd BLAST496
ChainiPRO_00003381633247 – 35493C-like proteinaseBy similarityAdd BLAST303
ChainiPRO_00003381643550 – 3836Non-structural protein 6By similarityAdd BLAST287
ChainiPRO_00003381653837 – 3925Non-structural protein 7By similarityAdd BLAST89
ChainiPRO_00003381663926 – 4122Non-structural protein 8By similarityAdd BLAST197
ChainiPRO_00003381674123 – 4232Non-structural protein 9By similarityAdd BLAST110
ChainiPRO_00003381684233 – 4369Non-structural protein 10By similarityAdd BLAST137
ChainiPRO_00003381694370 – 4383Non-structural protein 11Sequence analysisAdd BLAST14

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei246 – 247Cleavage; by PL1-PROBy similarity2
Sitei851 – 852Cleavage; by PL1-PROBy similarity2
Sitei2750 – 2751Cleavage; by PL2-PROBy similarity2
Sitei3246 – 3247Cleavage; by 3CL-PROBy similarity2
Sitei3549 – 3550Cleavage; by 3CL-PROBy similarity2
Sitei3836 – 3837Cleavage; by 3CL-PROBy similarity2
Sitei3925 – 3926Cleavage; by 3CL-PROBy similarity2
Sitei4122 – 4123Cleavage; by 3CL-PROBy similarity2
Sitei4232 – 4233Cleavage; by 3CL-PROBy similarity2
Sitei4369 – 4370Cleavage; by 3CL-PROBy similarity2

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).

By similarity

Protein-protein interaction databases

Protein interaction database and analysis system

More...IntActi		P0C6U0, 1 interactor

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0C6U0

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1036 – 1274Peptidase C16 1PROSITE-ProRule annotationAdd BLAST239
Domaini1275 – 1435MacroPROSITE-ProRule annotationAdd BLAST161
Domaini1631 – 1892Peptidase C16 2PROSITE-ProRule annotationAdd BLAST262
Domaini3247 – 3549Peptidase C30PROSITE-ProRule annotationAdd BLAST303

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni2138 – 2385HD1Add BLAST248
Regioni2752 – 3135HD2Add BLAST384
Regioni3558 – 3775HD3Add BLAST218

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the coronaviruses polyprotein 1ab family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1151 – 1179C4-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri1749 – 1785C4-type 2PROSITE-ProRule annotationAdd BLAST37
Zinc fingeri4306 – 4322By similarityAdd BLAST17
Zinc fingeri4348 – 4361By similarityAdd BLAST14

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.150.420, 1 hit
1.10.1840.10, 1 hit
1.10.8.1190, 1 hit
1.10.8.370, 1 hit
2.40.10.10, 2 hits
2.40.10.250, 1 hit
2.40.10.290, 1 hit
3.10.20.350, 1 hit
3.10.20.540, 1 hit
3.40.220.10, 1 hit
3.90.70.90, 2 hits

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR022570, B-CoV_NSP1
IPR043613, CoV_NSP2_C
IPR043611, CoV_NSP3_C
IPR043612, CoV_NSP4_N
IPR043610, CoV_NSP6
IPR002589, Macro_dom
IPR043472, Macro_dom-like
IPR036333, NSP10_sf_CoV
IPR032592, NSP3_NAR_bCoV
IPR038083, NSP3A-like
IPR032505, NSP4_C_CoV
IPR038123, NSP4_C_sf_CoV
IPR014828, NSP7_CoV
IPR037204, NSP7_sf_CoV
IPR014829, NSP8_CoV-like
IPR037230, NSP8_sf_CoV
IPR014822, NSP9_CoV
IPR036499, NSP9_sf_CoV
IPR002705, Pept_C30/C16_B_coronavir
IPR013016, Peptidase_C16_CoV
IPR008740, Peptidase_C30_CoV
IPR043477, Peptidase_C30_dom3_CoV
IPR009003, Peptidase_S1_PA
IPR043504, Peptidase_S1_PA_chymotrypsin
IPR043177, PLpro_N_sf_CoV
IPR043503, PLpro_palm_finger_dom_CoV
IPR043178, PLpro_thumb_sf_CoV
IPR018995, RNA_synth_NSP10_CoV

Pfam protein domain database

More...Pfami		View protein in Pfam
PF16251, bCoV_NAR, 1 hit
PF09401, CoV_NSP10, 1 hit
PF19212, CoV_NSP2_C, 1 hit
PF19218, CoV_NSP3_C, 1 hit
PF16348, CoV_NSP4_C, 1 hit
PF19217, CoV_NSP4_N, 1 hit
PF19213, CoV_NSP6, 1 hit
PF08716, CoV_NSP7, 1 hit
PF08717, CoV_NSP8, 1 hit
PF08710, CoV_NSP9, 1 hit
PF08715, CoV_peptidase, 1 hit
PF11963, DUF3477, 1 hit
PF01661, Macro, 1 hit
PF01831, Peptidase_C16, 1 hit
PF05409, Peptidase_C30, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00506, A1pp, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF101816, SSF101816, 1 hit
SSF140367, SSF140367, 1 hit
SSF143076, SSF143076, 1 hit
SSF144246, SSF144246, 1 hit
SSF159936, SSF159936, 1 hit
SSF50494, SSF50494, 1 hit
SSF52949, SSF52949, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51442, M_PRO, 1 hit
PS51154, MACRO, 1 hit
PS51124, PEPTIDASE_C16, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by ribosomal frameshifting. AlignAdd to basket
Isoform Replicase polyprotein 1a (identifier: P0C6U0-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSKINKYGLE LHWAPEFPWM FEDAEEKLDN PSSSEVDIVC STTAQKLETG 
        60         70         80         90        100
GICPENHVMV DCRRLLKQEC CVQSSLIREI VMNTRPYDLE VLLQDALQSR 
       110        120        130        140        150
EAVLVTPPLG MSLEACYVRG CNPNGWTMGL FRRRSVCNTG RCAVNKHVAY 
       160        170        180        190        200
QLYMIDPAGV CFGAGQFVGW VIPLAFMPVQ SRKFIVPWVM YLRKCGEKGA 
       210        220        230        240        250
YNKDHKRGGF EHVYNFKVED AYDLVHDEPK GKFSKKAYAL IRGYRGVKPL 
       260        270        280        290        300
LYVDQYGCDY TGGLADGLEA YADKTLQEMK ALFPIWSQEL PFDVTVAWHV 
       310        320        330        340        350
VRDPRYVMRL QSASTIRSVA YVANPTEDLC DGSVVIKEPV HVYADDSIIL 
       360        370        380        390        400
RQHNLVDIMS CFYMEADAVV NAFYGVDLKD CGFVMQFGYI DCEQDLCDFK 
       410        420        430        440        450
GWVPGNMIDG FACTTCGHVY ETGDLLAQSS GVLPVNPVLH TKSAAGYGGF 
       460        470        480        490        500
GCKDSFTLYG QTVVYFGGCV YWSPARNIWI PILKSSVKSY DGLVYTGVVG 
       510        520        530        540        550
CKAIVKETNL ICKALYLDYV QHKCGNLHQR ELLGVSDVWH KQLLLNRGVY 
       560        570        580        590        600
KPLLENIDYF NMRRAKFSLE TFTVCADGFM PFLLDDLVPR AYYLAVSGQA 
       610        620        630        640        650
FCDYAGKICH AVVSKSKELL DVSLDSLGAA IHYLNSKIVD LAQHFSDFGT 
       660        670        680        690        700
SFVSKIVHFF KTFTTSTALA FAWVLFHVLH GAYIVVESDI YFVKNIPRYA 
       710        720        730        740        750
SAVAQAFRSV AKVVLDSLRV TFIDGLSCFK IGRRRICLSG SKIYEVERGL 
       760        770        780        790        800
LHSSQLPLDV YDLTMPSQVQ KTKQKPIYLK GSGSDFSLAD SVVEVVTTSL 
       810        820        830        840        850
TPCGYSEPPK VADKICIVDN VYMAKAGDKY YPVVVDGHVG LLDQAWRVPC 
       860        870        880        890        900
AGRCVTFKEQ PTVNEIASTP KTIKVFYELD KDFNTILNTA CGVFEVDDTV 
       910        920        930        940        950
DMEEFYAVVI DAIEEKLSPC KELEGVGAKV SAFLQKLEDN SLFLFDEAGE 
       960        970        980        990       1000
EVLAPKLYCA FTAPEDDDFL EESGVEEDDV EGEETDLTVT SAGEPCVASE 
      1010       1020       1030       1040       1050
QEESSEILED TLDDGPCVET SDSQVEEDVQ MSDFVDLESV IQDYENVCFE 
      1060       1070       1080       1090       1100
FYTTEPEFVK VLDLYVPKAT RNNCWLRSVL AVMQKLPCQF KDKNLQDLWV 
      1110       1120       1130       1140       1150
LYKQQYSQLF VDTLVNKIPA NIVVPQGGYV ADFAYWFLTL CDWQCVAYWK 
      1160       1170       1180       1190       1200
CIKCDLALKL KGLDAMFFYG DVVSHVCKCG ESMVLIDVDV PFTAHFALKD 
      1210       1220       1230       1240       1250
KLFCAFITKR SVYKAACVVD VNDSHSMAVV DGKQIDDHRI TSITSDKFDF 
      1260       1270       1280       1290       1300
IIGHGMSFSM TTFEIAQLYG SCITPNVCFV KGDIIKVSKR VKAEVVVNPA 
      1310       1320       1330       1340       1350
NGHMAHGGGV AKAIAVAAGQ QFVKETTDMV KSKGVCATGD CYVSTGGKLC 
      1360       1370       1380       1390       1400
KTVLNVVGPD ARTQGKQSYA LLERVYKHLN KYDCVVTTLI SAGIFSVPSD 
      1410       1420       1430       1440       1450
VSLTYLLGTA KKQVVLVSNN QEDFDLISKC QITAVEGTKK LAERLSFNVG 
      1460       1470       1480       1490       1500
RSIVYETDAN KLILSNDVAF VSTFNVLQDV LSLRHDIALD DDARTFVQSN 
      1510       1520       1530       1540       1550
VDVVPEGWRV VNKFYQINGV RTVKYFECPG GIDICSQDKV FGYVQQGSFN 
      1560       1570       1580       1590       1600
KATVAQIKAL FLDKVDILLT VDGVNFTNRF VPVGESFGKS LGNVFCDGVN 
      1610       1620       1630       1640       1650
VTKHKCDINY KGKVFFQFDN LSSEDLKAVR SSFNFDQKEL LAYYNMLVNC 
      1660       1670       1680       1690       1700
SKWQVVFNGK YFTFKQANNN CFVNVSCLML QSLNLKFKIV QWQEAWLEFR 
      1710       1720       1730       1740       1750
SGRPARFVSL VLAKGGFKFG DPADSRDFLR VVFSQVDLTG AICDFEIACK 
      1760       1770       1780       1790       1800
CGVKQEQRTG VDAVMHFGTL SREDLEIGYT VDCSCGKKLI HCVRFDVPFL 
      1810       1820       1830       1840       1850
ICSNTPASVK LPKGVGSANI FKGDKVGHYV HVKCEQSYQL YDASNVKKVT 
      1860       1870       1880       1890       1900
DVTGNLSDCL YLKNLKQTFK SVLTTYYLDD VKKIEYKPDL SQYYCDGGKY 
      1910       1920       1930       1940       1950
YTQRIIKAQF KTFEKVDGVY TNFKLIGHTV CDILNAKLGF DSSKEFVEYK 
      1960       1970       1980       1990       2000
VTEWPTATGD VVLATDDLYV KRYERGCITF GKPVIWLSHE QASLNSLTYF 
      2010       2020       2030       2040       2050
NRPLLVDENK FDVLKVDDVD DGGDISESDA KEPKEINIIK LSGVKKPFKV 
      2060       2070       2080       2090       2100
EDSVIVNDDT SEIKYVKSLS IVDVYDMWLT GCRCVVRTAN ALSRAVNVPT 
      2110       2120       2130       2140       2150
IRKFIKFGMT LVSIPIDLLN LREIKPVFNV VKAVRNKISA CFNFIKWLFV 
      2160       2170       2180       2190       2200
LLFGWIKISA DNKVIYTTEV ASKLTCKLVA LAFKNAFLTF KWSVVARGAC 
      2210       2220       2230       2240       2250
IIATIFLLWF NFIYANVIFS DFYLPKIGFL PTFVGKIAQW IKNTFSLVTI 
      2260       2270       2280       2290       2300
CDLYSIQDVG FKNQYCNGSI ACQFCLAGFD MLDNYKAIDV VQYEADRRAF 
      2310       2320       2330       2340       2350
VDYTGVLKIV IELIVSYALY TAWFYPLFAL ISIQILTTWL PELFMLSTLH 
      2360       2370       2380       2390       2400
WSVRLLVSLA NMLPAHVFMR FYIIIASFIK LFSLFRHVAY GCSKSGCLFC 
      2410       2420       2430       2440       2450
YKRNRSLRVK CSTIVGGMIR YYDAMANGGT GFCSKHQWNC IDCDSYKPGN 
      2460       2470       2480       2490       2500
TFITVEAALD LSKELKRPIQ PTDVAYHTVT DVKQVGCYMR LFYDRDGQRT 
      2510       2520       2530       2540       2550
YDDVNASLFV DYSNLLHSKV KSVPNMHVVV VENDADKANF LNAAVFYAQS 
      2560       2570       2580       2590       2600
LFRPILMVDK NLITTANTGT SVTETMFDVY VDTFLSMFDV DKKSLNALIA 
      2610       2620       2630       2640       2650
TAHSSIKQGT QICKVLDTFL SCARKSCSID SDVDTKCLAD SVMSAVSAGL 
      2660       2670       2680       2690       2700
ELTDESCNNL VPTYLKGDNI VAADLGVLIQ NSAKHVQGNV AKIAGVSCIW 
      2710       2720       2730       2740       2750
SVDAFNQLSS DFQHKLKKAC CKTGLKLKLT YNKQMANVSV LTTPFSLKGG 
      2760       2770       2780       2790       2800
AVFSYFVYVC FVLSLVCFIG LWCLMPTYTV HKSDFQLPVY ASYKVLDNGV 
      2810       2820       2830       2840       2850
IRDVSVEDVC FANKFEQFDQ WYESTFGLSY YSNSMACPIV VAVVDQDFGS 
      2860       2870       2880       2890       2900
TVFNVPTKVL RYGYHVLHFI THALSADGVQ CYTPHSQISY SNFYASGCVL 
      2910       2920       2930       2940       2950
SSACTMFAMA DGSPQPYCYT DGLMQNASLY SSLVPHVRYN LANAKGFIRF 
      2960       2970       2980       2990       3000
PEVLREGLVR IVRTRSMPYC RVGLCEEADE GICFNFNGSW VLNNDYYRSL 
      3010       3020       3030       3040       3050
PGTFCGRDVF DLIYQLFKGL AQPVDFLALT ASSIAGAILA VIVVLVFYYL 
      3060       3070       3080       3090       3100
IKLKRAFGDY TSIVFVNVIV WCVNFMMLFV FQVYPTLSCV YAICYFYATL 
      3110       3120       3130       3140       3150
YFPSEISVIM HLQWLVMYGT IMPLWFCLLY ISVVVSNHAF WVFSYCRQLG 
      3160       3170       3180       3190       3200
TSVRSDGTFE EMALTTFMIT KDSYCKLKNS LSDVAFNRYL SLYNKYRYYS 
      3210       3220       3230       3240       3250
GKMDTAAYRE AACSQLAKAM DTFTNNNGSD VLYQPPTASV STSFLQSGIV 
      3260       3270       3280       3290       3300
KMVNPTSKVE PCIVSVTYGN MTLNGLWLGD KVYCPRHVIC SASDMTNPDY 
      3310       3320       3330       3340       3350
TNLLCRVTSS DFTVLFDRLS LTVMSYQMQG CMLVLTVTLQ NSRTPKYTFG 
      3360       3370       3380       3390       3400
VVKPGETFTV LAAYNGKPQG AFHVTMRSSY TIKGSFLCGS CGSVVYVIMG 
      3410       3420       3430       3440       3450
DCVKFVYMHQ LELSTGCHTG TDFNGDFYGP YKDAQVVQLP VQDYIQSVNF 
      3460       3470       3480       3490       3500
VAWLYAAILN NCNWFVQSDK CSVEDFNVWA LSNGFSQVKS DLVIDALASM 
      3510       3520       3530       3540       3550
TGVSLETLLA AIKHLKNGFQ GRQIMGSCSF EDELTPSDVY QQLAGIKLQS 
      3560       3570       3580       3590       3600
KRTRLVKGIV CWIMASTFLF SCIITAFVKW TMFMYVTTNM LSITFCALCV 
      3610       3620       3630       3640       3650
ISLTMLLVKH KHLYLTMYII PVLFTLLYNN YLVVYKQTFR GYVYAWLSYY 
      3660       3670       3680       3690       3700
VPSVEYTYTD EVIYGMLLLI GMVFVTLRSI NQYLFSFIMF VGRVISVVSL 
      3710       3720       3730       3740       3750
WYMGSNLEEE ILLMLASLFG TYTWTTALSM AAAKVIAKWV AVNVLYFTDI 
      3760       3770       3780       3790       3800
PQIKIVLVCY LFIGYIISCY WGLFSLMNSL FRMPLGVYNY KISVQELRYM 
      3810       3820       3830       3840       3850
NANGLRPPKN SFEALMLNFK LLGIGGVPII EVSQFQSKLT DVKCANVVLL 
      3860       3870       3880       3890       3900
NCLQHLHVAS NSKLWQYCST LHNEILATSD LGVAFEKLAQ LLIVLFANPA 
      3910       3920       3930       3940       3950
AVDSKCLTSI EEVCDDYAKD NTVLQALQSE FVNMASFVEY EVAKKNLDEA 
      3960       3970       3980       3990       4000
CSSGSANQQQ LKQLEKACNI AKSAYERDRA VARKLERMAD LALTNMYKEA 
      4010       4020       4030       4040       4050
RINDKKSKVV SALQTMLFSM VRKLDNQALN SILDNAVKGC VPLNAIPSLA 
      4060       4070       4080       4090       4100
ANTLTIIVPD KSVYDQVVDN VYVTYAGNVW QIQTIQDSDG TNKQLHEISD 
      4110       4120       4130       4140       4150
DCNWPLVIIA NRHNEVSATA LQNNELMPAK LKTQVVNSGP DQTCNTPTQC 
      4160       4170       4180       4190       4200
YYNNSYNGKI VYAILSDVDG LKYTKILKDD GNFVVLELDP PCKFTVQDVK 
      4210       4220       4230       4240       4250
GLKIKYLYFV KGCNTLARGW VVGTISSTVR LQAGTATEYA SNSSILSLCA 
      4260       4270       4280       4290       4300
FSVDPKKTYL DFIQQGGTPI ANCVKMLCDH AGTGMAITVK PDATTSQDSY 
      4310       4320       4330       4340       4350
GGASVCIYCR ARVEHPDVDG LCKLRGKFVQ VPVGIKDPVS YVLTHDVCQV 
      4360       4370       4380 
CGFWRDGSCS CVSTDTTVQS KDTNFLNGFG VRV
Note: Produced by conventional translation.
Length:4,383
Mass (Da):490,562
Last modified:June 10, 2008 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3B4FBFA0C0C7EE34
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6W9-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6W9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:7,094
Mass (Da):797,392
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U00735 Genomic RNA No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00735 Genomic RNA No translation available.

3D structure databases

SMRiP0C6U0
ModBaseiSearch...

Protein-protein interaction databases

IntActiP0C6U0, 1 interactor

Family and domain databases

Gene3Di1.10.150.420, 1 hit
1.10.1840.10, 1 hit
1.10.8.1190, 1 hit
1.10.8.370, 1 hit
2.40.10.10, 2 hits
2.40.10.250, 1 hit
2.40.10.290, 1 hit
3.10.20.350, 1 hit
3.10.20.540, 1 hit
3.40.220.10, 1 hit
3.90.70.90, 2 hits
InterProiView protein in InterPro
IPR022570, B-CoV_NSP1
IPR043613, CoV_NSP2_C
IPR043611, CoV_NSP3_C
IPR043612, CoV_NSP4_N
IPR043610, CoV_NSP6
IPR002589, Macro_dom
IPR043472, Macro_dom-like
IPR036333, NSP10_sf_CoV
IPR032592, NSP3_NAR_bCoV
IPR038083, NSP3A-like
IPR032505, NSP4_C_CoV
IPR038123, NSP4_C_sf_CoV
IPR014828, NSP7_CoV
IPR037204, NSP7_sf_CoV
IPR014829, NSP8_CoV-like
IPR037230, NSP8_sf_CoV
IPR014822, NSP9_CoV
IPR036499, NSP9_sf_CoV
IPR002705, Pept_C30/C16_B_coronavir
IPR013016, Peptidase_C16_CoV
IPR008740, Peptidase_C30_CoV
IPR043477, Peptidase_C30_dom3_CoV
IPR009003, Peptidase_S1_PA
IPR043504, Peptidase_S1_PA_chymotrypsin
IPR043177, PLpro_N_sf_CoV
IPR043503, PLpro_palm_finger_dom_CoV
IPR043178, PLpro_thumb_sf_CoV
IPR018995, RNA_synth_NSP10_CoV
PfamiView protein in Pfam
PF16251, bCoV_NAR, 1 hit
PF09401, CoV_NSP10, 1 hit
PF19212, CoV_NSP2_C, 1 hit
PF19218, CoV_NSP3_C, 1 hit
PF16348, CoV_NSP4_C, 1 hit
PF19217, CoV_NSP4_N, 1 hit
PF19213, CoV_NSP6, 1 hit
PF08716, CoV_NSP7, 1 hit
PF08717, CoV_NSP8, 1 hit
PF08710, CoV_NSP9, 1 hit
PF08715, CoV_peptidase, 1 hit
PF11963, DUF3477, 1 hit
PF01661, Macro, 1 hit
PF01831, Peptidase_C16, 1 hit
PF05409, Peptidase_C30, 1 hit
SMARTiView protein in SMART
SM00506, A1pp, 1 hit
SUPFAMiSSF101816, SSF101816, 1 hit
SSF140367, SSF140367, 1 hit
SSF143076, SSF143076, 1 hit
SSF144246, SSF144246, 1 hit
SSF159936, SSF159936, 1 hit
SSF50494, SSF50494, 1 hit
SSF52949, SSF52949, 1 hit
PROSITEiView protein in PROSITE
PS51442, M_PRO, 1 hit
PS51154, MACRO, 1 hit
PS51124, PEPTIDASE_C16, 2 hits

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiR1A_CVBM
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0C6U0
Secondary accession number(s): Q66198
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: December 2, 2020
This is version 74 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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