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UniProtKB - P0C6T5 (R1A_BCHK5)

Entry version 84 (02 Dec 2020)
Sequence version 1 (10 Jun 2008)
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Protein

Replicase polyprotein 1a

Gene

1a

Organism
Bat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. EC:3.4.22.69
  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei1668For PL-PRO activityPROSITE-ProRule annotation1
Active sitei1838For PL-PRO activityPROSITE-ProRule annotation1
Active sitei3379For 3CL-PRO activityPROSITE-ProRule annotation1
Active sitei3486For 3CL-PRO activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri1748 – 1785C4-typePROSITE-ProRule annotationAdd BLAST38
Zinc fingeri4402 – 4418By similarityAdd BLAST17
Zinc fingeri4444 – 4457By similarityAdd BLAST14

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, RNA-binding, Thiol protease
Biological processActivation of host autophagy by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion
LigandMetal-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Non-structural protein 3 (EC:3.4.19.12, EC:3.4.22.69)
Short name:
nsp3
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
Non-structural protein 11
Short name:
nsp11
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
ORF Names:1a
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri694008 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiViruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Merbecovirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiPipistrellus abramus (Japanese pipistrelle) (Pipistrellus javanicus abramus) [TaxID: 105295]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000007451 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

  • host perinuclear region By similarity
    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
  • host perinuclear region By similarity
    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
  • host perinuclear region By similarity
    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
  • host perinuclear region By similarity
    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei2196 – 2216HelicalSequence analysisAdd BLAST21
Transmembranei2268 – 2288HelicalSequence analysisAdd BLAST21
Transmembranei2372 – 2392HelicalSequence analysisAdd BLAST21
Transmembranei2396 – 2416HelicalSequence analysisAdd BLAST21
Transmembranei2421 – 2441HelicalSequence analysisAdd BLAST21
Transmembranei2848 – 2868HelicalSequence analysisAdd BLAST21
Transmembranei3119 – 3139HelicalSequence analysisAdd BLAST21
Transmembranei3152 – 3172HelicalSequence analysisAdd BLAST21
Transmembranei3203 – 3223HelicalSequence analysisAdd BLAST21
Transmembranei3650 – 3670HelicalSequence analysisAdd BLAST21
Transmembranei3684 – 3704HelicalSequence analysisAdd BLAST21
Transmembranei3709 – 3729HelicalSequence analysisAdd BLAST21
Transmembranei3760 – 3777HelicalSequence analysisAdd BLAST18
Transmembranei3782 – 3802HelicalSequence analysisAdd BLAST21
Transmembranei3823 – 3843HelicalSequence analysisAdd BLAST21
Transmembranei3855 – 3875HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003380981 – 4481Replicase polyprotein 1aAdd BLAST4481
ChainiPRO_00003380991 – 195Non-structural protein 1Sequence analysisAdd BLAST195
ChainiPRO_0000338100196 – 851Non-structural protein 2Sequence analysisAdd BLAST656
ChainiPRO_0000338101852 – 2830Non-structural protein 3Sequence analysisAdd BLAST1979
ChainiPRO_00003381022831 – 3338Non-structural protein 4Sequence analysisAdd BLAST508
ChainiPRO_00003381033339 – 36443C-like proteinaseSequence analysisAdd BLAST306
ChainiPRO_00003381043645 – 3936Non-structural protein 6Sequence analysisAdd BLAST292
ChainiPRO_00003381053937 – 4019Non-structural protein 7Sequence analysisAdd BLAST83
ChainiPRO_00003381064020 – 4218Non-structural protein 8Sequence analysisAdd BLAST199
ChainiPRO_00003381074219 – 4328Non-structural protein 9Sequence analysisAdd BLAST110
ChainiPRO_00003381094328 – 4481Non-structural protein 11Sequence analysisAdd BLAST154
ChainiPRO_00003381084329 – 4467Non-structural protein 10Sequence analysisAdd BLAST139

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei195 – 196CleavageSequence analysis2
Sitei851 – 852Cleavage; by PL-PROSequence analysis2
Sitei2830 – 2831Cleavage; by PL-PROSequence analysis2
Sitei3338 – 3339Cleavage; by 3CL-PROSequence analysis2
Sitei3644 – 3645Cleavage; by 3CL-PROSequence analysis2
Sitei3936 – 3937Cleavage; by 3CL-PROSequence analysis2
Sitei4019 – 4020Cleavage; by 3CL-PROSequence analysis2
Sitei4218 – 4219Cleavage; by 3CL-PROSequence analysis2
Sitei4328 – 4329Cleavage; by 3CL-PROSequence analysis2
Sitei4467 – 4468Cleavage; by 3CL-PROSequence analysis2

Proteomic databases

PRoteomics IDEntifications database

More...PRIDEi		P0C6T5

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).

By similarity

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0C6T5

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1186 – 1345MacroPROSITE-ProRule annotationAdd BLAST160
Domaini1628 – 1902Peptidase C16PROSITE-ProRule annotationAdd BLAST275
Domaini3339 – 3644Peptidase C30PROSITE-ProRule annotationAdd BLAST306

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni2158 – 2441HD1By similarityAdd BLAST284
Regioni2848 – 3223HD2By similarityAdd BLAST376
Regioni3650 – 3875HD3By similarityAdd BLAST226

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi964 – 1066Glu-richAdd BLAST103

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the coronaviruses polyprotein 1ab family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1748 – 1785C4-typePROSITE-ProRule annotationAdd BLAST38
Zinc fingeri4402 – 4418By similarityAdd BLAST17
Zinc fingeri4444 – 4457By similarityAdd BLAST14

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.150.420, 1 hit
1.10.1840.10, 1 hit
1.10.8.1190, 1 hit
1.10.8.370, 1 hit
2.40.10.10, 2 hits
2.40.10.250, 1 hit
2.40.10.290, 1 hit
3.10.20.350, 1 hit
3.10.20.540, 1 hit
3.40.220.10, 1 hit
3.40.220.20, 1 hit
3.40.50.11020, 1 hit
3.90.70.90, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR043613, CoV_NSP2_C
IPR043615, CoV_NSP2_N
IPR043611, CoV_NSP3_C
IPR043612, CoV_NSP4_N
IPR043610, CoV_NSP6
IPR002589, Macro_dom
IPR043472, Macro_dom-like
IPR042570, NAR_sf
IPR036333, NSP10_sf_CoV
IPR021590, NSP1_bCoV
IPR024375, NSP3_bCoV
IPR032592, NSP3_NAR_bCoV
IPR038400, NSP3_SUD-M_sf_bCoV
IPR038083, NSP3A-like
IPR032505, NSP4_C_CoV
IPR038123, NSP4_C_sf_CoV
IPR014828, NSP7_CoV
IPR037204, NSP7_sf_CoV
IPR014829, NSP8_CoV-like
IPR037230, NSP8_sf_CoV
IPR014822, NSP9_CoV
IPR036499, NSP9_sf_CoV
IPR013016, Peptidase_C16_CoV
IPR008740, Peptidase_C30_CoV
IPR043477, Peptidase_C30_dom3_CoV
IPR009003, Peptidase_S1_PA
IPR043504, Peptidase_S1_PA_chymotrypsin
IPR043177, PLpro_N_sf_CoV
IPR043503, PLpro_palm_finger_dom_CoV
IPR043178, PLpro_thumb_sf_CoV
IPR018995, RNA_synth_NSP10_CoV

Pfam protein domain database

More...Pfami		View protein in Pfam
PF16251, bCoV_NAR, 1 hit
PF11501, bCoV_NSP1, 1 hit
PF11633, bCoV_SUD_M, 1 hit
PF09401, CoV_NSP10, 1 hit
PF19212, CoV_NSP2_C, 1 hit
PF19211, CoV_NSP2_N, 1 hit
PF19218, CoV_NSP3_C, 1 hit
PF16348, CoV_NSP4_C, 1 hit
PF19217, CoV_NSP4_N, 1 hit
PF19213, CoV_NSP6, 1 hit
PF08716, CoV_NSP7, 1 hit
PF08717, CoV_NSP8, 1 hit
PF08710, CoV_NSP9, 1 hit
PF08715, CoV_peptidase, 1 hit
PF01661, Macro, 1 hit
PF05409, Peptidase_C30, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00506, A1pp, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF101816, SSF101816, 1 hit
SSF140367, SSF140367, 1 hit
SSF143076, SSF143076, 1 hit
SSF144246, SSF144246, 1 hit
SSF159936, SSF159936, 1 hit
SSF50494, SSF50494, 1 hit
SSF52949, SSF52949, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51442, M_PRO, 1 hit
PS51154, MACRO, 1 hit
PS51124, PEPTIDASE_C16, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by ribosomal frameshifting. AlignAdd to basket
Isoform Replicase polyprotein 1a (identifier: P0C6T5-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSFVAGVAPQ GARGKYRAEL NTEKRTDHVS LKASLCDAGD LVLKISPWFM 
        60         70         80         90        100
DGESAYKHVS EQLSKGSKLL FVPQTLKGFI RHLPGPRVYL VERLTGGTYS 
       110        120        130        140        150
DPFMVNQLAY QNAAGEGVIG TTLQGKRVGM FFPFDADLVT GEFQFLLRKK 
       160        170        180        190        200
GFGGNRFRDA PWDYNWTPYS DLMDALEADP CGKYSQSLLK KLVGGDFTPI 
       210        220        230        240        250
DQYMCGKNGK PIAEFAALMA SEGITKLADV EAEVKSRTDS DRYIVFKNKL 
       260        270        280        290        300
YRIVWNVQRK DVAYSKQSAF TMNSIVQLDT MEDVPRHSFT IGSEIQVIAP 
       310        320        330        340        350
STAVQANGHL NLKQRLLYAF YGKQAVSEPN YIYHSAYVDC TSCGKGSWLT 
       360        370        380        390        400
GNAVQGFACD CGAHYCANDV DLQSSGLVRK NAVLLTTCPC NKDGECKHTL 
       410        420        430        440        450
PQLVSMMTDK CDVEVVGKTF ILTYGGVIYA YMGCSGGTMH FIPRAKSCVS 
       460        470        480        490        500
KIGDAIFTGC TGTWSKVCET ANLFLERAQH AINFVNEFVL TETVVALLSG 
       510        520        530        540        550
TTSSIEELRD LCRNATFEKV RDYLTPRGWI VTMGSYIEGV INVGAAGVCN 
       560        570        580        590        600
AALNAPFIVL SGLGESFKKV AATPWKLCSS LRETLDHYAD SITYRVFPYD 
       610        620        630        640        650
IPCDVTDYTA LLLDCAVLTG ASAYFVARYV DEKVEQLTNL VFSSCQSAVA 
       660        670        680        690        700
AFVQACMSTY KATAKFISDM FTLIKVVSER LYVYTSVGFV VVGDYSSQLL 
       710        720        730        740        750
KQFMHILSKA MQLLHTTVSW AGSKLPSVVY NGRDSLVFPS GTYYCVSTQG 
       760        770        780        790        800
RSLQDQFDLV IPGDLSKKQI GILEPTPNST TVDKKINTNV VEVVVGQLEP 
       810        820        830        840        850
TKEHSPELVV GDYVIISNKI FVRSVEDSET VFYPLCTDGK IVPTLFRLKG 
       860        870        880        890        900
GAPPKGVKFG GEQTKEITAV RSVSVDYDVH PVLDALLAGS ELATFTVEKD 
       910        920        930        940        950
LPVKDFVDVV KDEVIELLSK LLRGYNVDGF DLEDFADTPC YVYNAEGDLA 
       960        970        980        990       1000
WSSTMTFSVN PVEEVEEECD DDYVEDEYLS EEMLVEEDEN SWAAAVEAVI 
      1010       1020       1030       1040       1050
PMEDVQLDTL VAEIDVSEPA DDVAEQASTE EVEVPSACVL EASQVANAAE 
      1060       1070       1080       1090       1100
VESCEAEVSS SIPLHEDANA AKANDCAEGM PALDSTETVS KLSVDTPVGD 
      1110       1120       1130       1140       1150
VTQDDATSSN ATVISEDVHT ATHSKGLVAV PEVVPEKALG TSVERMRSTS 
      1160       1170       1180       1190       1200
EWTVVETSLK QETAVIVKND SSAKPQRVKK PKAENPLKNF KHIVLNNDVT 
      1210       1220       1230       1240       1250
LVFGDAIAVA RATEDCILVN AANTHLKHGG GIAAAIDRAS GGLVQAESDD 
      1260       1270       1280       1290       1300
YVNFYGPLNV GDSTLLKGHG LATGILHVVG PDARANQDIQ LLKRCYKAFN 
      1310       1320       1330       1340       1350
KYPLVVSPLI SAGIFCVEPR VSLEYLLSVV HTKTYVVVNS EKVYNDLAAP 
      1360       1370       1380       1390       1400
KPPTGLTYSH EGWRGIIRNA KSFGFTCFIC TDQSANAKLL KGRGVDLTKK 
      1410       1420       1430       1440       1450
TQTVDGVKYY LYSSKDPLTD IITAANACKG ICAMPIGYVT HGLDLAQAGQ 
      1460       1470       1480       1490       1500
QVKKITVPYV CLLASKDQVP ILNSDVAVQT PEQSFINTVI ANGGYHCWHL 
      1510       1520       1530       1540       1550
VTGELIVKGV SYRKLLNWSD QTICYADNKF YVVKGQIALP FDSLEKCRTY 
      1560       1570       1580       1590       1600
LTSRAAQQKN VDVLVTIDGV NFRTVVLNNT TTYRVQLGSV FYKGSDISDT 
      1610       1620       1630       1640       1650
IPTEKMSGEA VYLADNLSEA EKAVLSEVYG TADTAFLHRY YSLLALVKKW 
      1660       1670       1680       1690       1700
KYTVHDGVKS LKLNSNNCYV NVTMLMLDML KEIKFIVPAL QAAYLKHKGG 
      1710       1720       1730       1740       1750
DSTEFIALIM AYGDCTYGEP DDASRLLHTI LSKAELTTQA KMVWRQWCNV 
      1760       1770       1780       1790       1800
CGVQDTTTTG LKACIYVGMN SLDELHATHE ECCQCGDVRK RQLVEHNAPW 
      1810       1820       1830       1840       1850
LLLSGLNEAK VMTPTSQSAG PDYTAFNVFQ GVETSVGHYL HVRVKDNLLY 
      1860       1870       1880       1890       1900
KYDSGSLSKT SDMKCKMTDV YYPKQRYSAD CNVVVYSLDG NTWADVDPDL 
      1910       1920       1930       1940       1950
SAFYMKDGKY FTKKPVIEYS PATILSGSVY TNSCLVGHDG TIGSDAISSS 
      1960       1970       1980       1990       2000
FNNLLGFDNS KPVSKKLTYS FFPDFEGDVI LTEYSTYDPI YKNGAMLHGK 
      2010       2020       2030       2040       2050
PILWVNNSKF DSALNKFNRA TLRQVYDIAP VTLENKYTVL QDNQIQQVEV 
      2060       2070       2080       2090       2100
EAPKEDAKPQ SPVQVAEDID NKLPIIKCKG LKKPFVKDGY SFVNDPQGVN 
      2110       2120       2130       2140       2150
VIDTLGIDDL RALYVDRNLR LIVLKENNWS ALFNIHTVEK GDLSVIAASG 
      2160       2170       2180       2190       2200
SITRRVKILL GASSLFAQFA SVTVNVTTAM GKALGRMTRN VITNTGIIGQ 
      2210       2220       2230       2240       2250
GFALLKMLLI LPFTFWKSKN QSTVKVEVGA LRTAGIVTTN VVKQCASAAY 
      2260       2270       2280       2290       2300
DVLVVKFKRI DWKSTLRLLF LICTTGLLLS SLYYLFLFHQ VLTSDVMLDG 
      2310       2320       2330       2340       2350
AEGMLATYRE LRSYLGIHSL CDGMVEAYRN VSYDVNDFCS NRSALCNWCL 
      2360       2370       2380       2390       2400
IGQDSLTRYS AFQMIQTHVT SYVINIDWVW FVMEFALAYV LYTSTFNVLL 
      2410       2420       2430       2440       2450
LVVSSQYFFS YTGAFVNWRS YNYLVSGYFF CVTHIPLLGL VRIYNFLACL 
      2460       2470       2480       2490       2500
WFLRRFYNHV INGCKDTACL LCYKRNRLTR VEASTVVCGS KRTFYIVANG 
      2510       2520       2530       2540       2550
GTSFCCRHNW NCVDCDTAGI GNTFICEEVA NDLTTSLRRL VKPTDKSHYY 
      2560       2570       2580       2590       2600
VESVTVKDSV VQLHYSREGA SCYERYPLCY FTNLDKLKFK EVCKTPTGIP 
      2610       2620       2630       2640       2650
EHNFLIYDSS DRGQENLARS ACVYYSQVLS KPMLLVDSNM VTTVGDSREI 
      2660       2670       2680       2690       2700
ASKMLDSYVN SFISLFGVNR DKLDKLVATA RDCVKRGDDF QTVIKTFTDA 
      2710       2720       2730       2740       2750
ARGPAGVESD VETSSIVDAL QYAYKHDLQL TTEGFNNYVP SYIKPDSVAT 
      2760       2770       2780       2790       2800
ADLGCLIDLN AASVNQTSIR NANGACIWNS SDYMKLSDSL KRQIRIACRK 
      2810       2820       2830       2840       2850
CNIPFRLTTS RLRSADNILS VKFSATKLSG GAPKWLLKLR DFTWKSYCVV 
      2860       2870       2880       2890       2900
TLVVFAMAVL SYLCLPAFNM SQVSFHEDRI LTYKVVENGI IRDITPSDTC 
      2910       2920       2930       2940       2950
FANKYQSFSK WFNEHYGGLF NNDISCPVTV AVIAGVAGAR VPNLPANVAW 
      2960       2970       2980       2990       3000
VGRQIVLFVS RVFASSNNVC YTPTAEIPYE RFSDSGCVLA SECTLFRDAE 
      3010       3020       3030       3040       3050
GKINPYCYDP TVLPGASAYD QMKPHVRYDM YDSDMYIKFP EVVFESTLRI 
      3060       3070       3080       3090       3100
TKTLATRYCR FGSCEDANEG VCITTNGSWA IYNDHYANKP GVYCGDNYFD 
      3110       3120       3130       3140       3150
IVRRLGLSLF QPVTYFQLST SLALGVMLCI FLTIAFYYVN KVKRALADYT 
      3160       3170       3180       3190       3200
QCAVVAVAAA LLNSLCLCFV VSNPLLVLPY TALYYYATFY LTGEPAFVMH 
      3210       3220       3230       3240       3250
VSWFVMFGTV VPIWMVFAYI VGVCLRHLLW VMAYFSKKHV EVFTDGKLNC 
      3260       3270       3280       3290       3300
SFQDAAANIF VINKDTYVAL RNSITQDSYN RYLSMFNKYK YYSGAMDTAS 
      3310       3320       3330       3340       3350
YREASAAHLC KALQVYSETG SDVLFQPPNC SVTSSVLQSG LVKMAAPSGV 
      3360       3370       3380       3390       3400
VENCMVQVTC GSMTLNGLWL DNYVWCPRHV MCPADQLSDP NYDALLVSKT 
      3410       3420       3430       3440       3450
NLSFIVQKNV GAPANLRVVG HTMVGTLLKL TVESANPQTP AYTFTTVKPG 
      3460       3470       3480       3490       3500
ASFSVLACYN GRPTGVFMVN MRQNSTIKGS FLCGSCGSVG YTQEGNVINF 
      3510       3520       3530       3540       3550
CYMHQMELSN GTHTGCAFDG VMYGAFEDRQ VHQVQLSDKY CTINIVAWLY 
      3560       3570       3580       3590       3600
AAILNGCNWF VKPNKTGIAT FNEWAMSNQF TEFIGTQSVD MLAHKTGVSV 
      3610       3620       3630       3640       3650
EQLLYAIQTL HKGFQGKTIL GNSMLEDEFT PDDVNMQVMG VVMQSGVKRI 
      3660       3670       3680       3690       3700
SYGLVHWLFT TLLLAYVATL QLTKFTIWNY LFEVIPLQLT PLVLCVMACV 
      3710       3720       3730       3740       3750
MLTVKHKHTF LTLFLLPTAI CLTYANIVYE PQTPVSSALI AVANWLNPAS 
      3760       3770       3780       3790       3800
VYMRTTHTDL GVYLSLCFAL AVVVRRLYRP NASNLALALG SAMVWFYTYT 
      3810       3820       3830       3840       3850
TGDCSSPLTY LMFLTTLTSD YTVTVFLAVN VAKFFARVVF LYAPHAGFIF 
      3860       3870       3880       3890       3900
PEVKLVLLMY LAVGYFCTVY FGVFSLLNLK LRVPLGVYDY TVSTQEFRYL 
      3910       3920       3930       3940       3950
TGNGLHAPRN SWEALRLNMK LIGIGGTPCI KIASVQSKLT DLKCTSVVLL 
      3960       3970       3980       3990       4000
SVLQQLHLEA NSKAWAHCVK LHNDILAATD PTEAFDNFVC LFATLMSFSA 
      4010       4020       4030       4040       4050
NVDLEALASD LLDHPSVLQA TLSEFSHLAS YAELEAAQSS YQKALNSGDA 
      4060       4070       4080       4090       4100
SPQVLKALQK AVNIAKNAYE KDKAVARKLE RMAEQAMTSM YKQARAEDKK 
      4110       4120       4130       4140       4150
AKIVSAMQTM LFGMIKKLDN DVLNGVISNA RNGCVPLSVV PLCASNKLRV 
      4160       4170       4180       4190       4200
VIPDITIWNK VVTWPSLSYA GALWDISLIN NVDGEVVKSS DVTETNESLT 
      4210       4220       4230       4240       4250
WPLVLECTRA ASSAVTLQNN EIRPSGLKTM VVSAGIDHAN CNTSSLAYYE 
      4260       4270       4280       4290       4300
PVEGRKMLMG ILSENAHLKW AKVEGRDGFV NIELQPPCKF LIAGPKGPEV 
      4310       4320       4330       4340       4350
RYLYFVKNLN NLHRGQLLGH IAATVRLQAG SNTEFAINSS VLSAVTFSVD 
      4360       4370       4380       4390       4400
PGKAYLDFVN AGGAPLTNCV KMLTPKTGTG IAVSVKPEAN ADQDTYGGAS 
      4410       4420       4430       4440       4450
VCLYCRAHIE HPDVTGVCKF KGKFVQVPLH IRDPVGFCLQ NTPCNVCQFW 
      4460       4470       4480 
IGHGCNCDAL RGTTIPQSKD SNFLNESGVL L
Note: Produced by conventional translation.
Length:4,481
Mass (Da):494,801
Last modified:June 10, 2008 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6EA797D2808AF75F
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6W4-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6W4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:7,182
Mass (Da):799,932
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
EF065509 Genomic RNA No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF065509 Genomic RNA No translation available.

3D structure databases

SMRiP0C6T5
ModBaseiSearch...

Proteomic databases

PRIDEiP0C6T5

Family and domain databases

Gene3Di1.10.150.420, 1 hit
1.10.1840.10, 1 hit
1.10.8.1190, 1 hit
1.10.8.370, 1 hit
2.40.10.10, 2 hits
2.40.10.250, 1 hit
2.40.10.290, 1 hit
3.10.20.350, 1 hit
3.10.20.540, 1 hit
3.40.220.10, 1 hit
3.40.220.20, 1 hit
3.40.50.11020, 1 hit
3.90.70.90, 1 hit
InterProiView protein in InterPro
IPR043613, CoV_NSP2_C
IPR043615, CoV_NSP2_N
IPR043611, CoV_NSP3_C
IPR043612, CoV_NSP4_N
IPR043610, CoV_NSP6
IPR002589, Macro_dom
IPR043472, Macro_dom-like
IPR042570, NAR_sf
IPR036333, NSP10_sf_CoV
IPR021590, NSP1_bCoV
IPR024375, NSP3_bCoV
IPR032592, NSP3_NAR_bCoV
IPR038400, NSP3_SUD-M_sf_bCoV
IPR038083, NSP3A-like
IPR032505, NSP4_C_CoV
IPR038123, NSP4_C_sf_CoV
IPR014828, NSP7_CoV
IPR037204, NSP7_sf_CoV
IPR014829, NSP8_CoV-like
IPR037230, NSP8_sf_CoV
IPR014822, NSP9_CoV
IPR036499, NSP9_sf_CoV
IPR013016, Peptidase_C16_CoV
IPR008740, Peptidase_C30_CoV
IPR043477, Peptidase_C30_dom3_CoV
IPR009003, Peptidase_S1_PA
IPR043504, Peptidase_S1_PA_chymotrypsin
IPR043177, PLpro_N_sf_CoV
IPR043503, PLpro_palm_finger_dom_CoV
IPR043178, PLpro_thumb_sf_CoV
IPR018995, RNA_synth_NSP10_CoV
PfamiView protein in Pfam
PF16251, bCoV_NAR, 1 hit
PF11501, bCoV_NSP1, 1 hit
PF11633, bCoV_SUD_M, 1 hit
PF09401, CoV_NSP10, 1 hit
PF19212, CoV_NSP2_C, 1 hit
PF19211, CoV_NSP2_N, 1 hit
PF19218, CoV_NSP3_C, 1 hit
PF16348, CoV_NSP4_C, 1 hit
PF19217, CoV_NSP4_N, 1 hit
PF19213, CoV_NSP6, 1 hit
PF08716, CoV_NSP7, 1 hit
PF08717, CoV_NSP8, 1 hit
PF08710, CoV_NSP9, 1 hit
PF08715, CoV_peptidase, 1 hit
PF01661, Macro, 1 hit
PF05409, Peptidase_C30, 1 hit
SMARTiView protein in SMART
SM00506, A1pp, 1 hit
SUPFAMiSSF101816, SSF101816, 1 hit
SSF140367, SSF140367, 1 hit
SSF143076, SSF143076, 1 hit
SSF144246, SSF144246, 1 hit
SSF159936, SSF159936, 1 hit
SSF50494, SSF50494, 1 hit
SSF52949, SSF52949, 1 hit
PROSITEiView protein in PROSITE
PS51442, M_PRO, 1 hit
PS51154, MACRO, 1 hit
PS51124, PEPTIDASE_C16, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiR1A_BCHK5
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0C6T5
Secondary accession number(s): A3EXC9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: December 2, 2020
This is version 84 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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