UniProtKB - P0C6T5 (R1A_BCHK5)
Replicase polyprotein 1a
1a
Functioni
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).
By similarityResponsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
PROSITE-ProRule annotationNsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
By similarityNsp9 is a ssRNA-binding protein.
By similarityCatalytic activityi
- TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. EC:3.4.22.69
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 340 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 343 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 359 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 361 | ZincPROSITE-ProRule annotation | 1 | |
Active sitei | 1668 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1838 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3379 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3486 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Metal bindingi | 4402 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4405 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4411 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4418 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4444 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4447 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4455 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4457 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1748 – 1785 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 4402 – 4418 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4444 – 4457 | By similarityAdd BLAST | 14 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: InterPro
- G-quadruplex RNA binding Source: InterPro
- single-stranded RNA binding Source: InterPro
- thiol-dependent deubiquitinase Source: UniProtKB-EC
- transferase activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- induction by virus of host autophagy Source: UniProtKB-KW
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- suppression by virus of host ISG15-protein conjugation Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
- suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity Source: UniProtKB-KW
- viral genome replication Source: InterPro
- viral protein processing Source: InterPro
Keywordsi
Enzyme and pathway databases
SABIO-RKi | P0C6T5 |
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1aShort name: pp1a Alternative name(s): ORF1a polyprotein Cleaved into the following 11 chains: Alternative name(s): Leader protein Alternative name(s): p65 homolog Alternative name(s): PL2-PRO Papain-like proteinase Short name: PL-PRO Alternative name(s): nsp5 Alternative name(s): Growth factor-like peptide Short name: GFL |
Gene namesi | ORF Names:1a |
Organismi | Bat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004) |
Taxonomic identifieri | 694008 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Merbecovirus |
Virus hosti | Pipistrellus abramus (Japanese pipistrelle) (Pipistrellus javanicus abramus) [TaxID: 105295] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 2196 – 2216 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2268 – 2288 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2372 – 2392 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2396 – 2416 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2421 – 2441 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2848 – 2868 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3119 – 3139 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3152 – 3172 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3203 – 3223 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3650 – 3670 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3684 – 3704 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3709 – 3729 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3760 – 3777 | HelicalSequence analysisAdd BLAST | 18 | |
Transmembranei | 3782 – 3802 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3823 – 3843 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3855 – 3875 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000338098 | 1 – 4481 | Replicase polyprotein 1aAdd BLAST | 4481 | |
ChainiPRO_0000338099 | 1 – 195 | Non-structural protein 1Sequence analysisAdd BLAST | 195 | |
ChainiPRO_0000338100 | 196 – 851 | Non-structural protein 2Sequence analysisAdd BLAST | 656 | |
ChainiPRO_0000338101 | 852 – 2830 | Non-structural protein 3Sequence analysisAdd BLAST | 1979 | |
ChainiPRO_0000338102 | 2831 – 3338 | Non-structural protein 4Sequence analysisAdd BLAST | 508 | |
ChainiPRO_0000338103 | 3339 – 3644 | 3C-like proteinaseSequence analysisAdd BLAST | 306 | |
ChainiPRO_0000338104 | 3645 – 3936 | Non-structural protein 6Sequence analysisAdd BLAST | 292 | |
ChainiPRO_0000338105 | 3937 – 4019 | Non-structural protein 7Sequence analysisAdd BLAST | 83 | |
ChainiPRO_0000338106 | 4020 – 4218 | Non-structural protein 8Sequence analysisAdd BLAST | 199 | |
ChainiPRO_0000338107 | 4219 – 4328 | Non-structural protein 9Sequence analysisAdd BLAST | 110 | |
ChainiPRO_0000338109 | 4328 – 4481 | Non-structural protein 11Sequence analysisAdd BLAST | 154 | |
ChainiPRO_0000338108 | 4329 – 4467 | Non-structural protein 10Sequence analysisAdd BLAST | 139 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 195 – 196 | CleavageSequence analysis | 2 | |
Sitei | 851 – 852 | Cleavage; by PL-PROSequence analysis | 2 | |
Sitei | 2830 – 2831 | Cleavage; by PL-PROSequence analysis | 2 | |
Sitei | 3338 – 3339 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 3644 – 3645 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 3936 – 3937 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 4019 – 4020 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 4218 – 4219 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 4328 – 4329 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 4467 – 4468 | Cleavage; by 3CL-PROSequence analysis | 2 |
Interactioni
Subunit structurei
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
By similarityProtein-protein interaction databases
IntActi | P0C6T5, 1 interactor |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 151 | CoV Nsp1 globularPROSITE-ProRule annotationAdd BLAST | 127 | |
Domaini | 159 – 195 | BetaCoV Nsp1 C-terminalPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 197 – 473 | CoV Nsp2 N-terminalPROSITE-ProRule annotationAdd BLAST | 277 | |
Domaini | 479 – 713 | CoV Nsp2 middlePROSITE-ProRule annotationAdd BLAST | 235 | |
Domaini | 715 – 851 | CoV Nsp2 C-terminalPROSITE-ProRule annotationAdd BLAST | 137 | |
Domaini | 855 – 964 | Ubiquitin-like 1PROSITE-ProRule annotationAdd BLAST | 110 | |
Domaini | 1186 – 1345 | Macro 1PROSITE-ProRule annotationAdd BLAST | 160 | |
Domaini | 1354 – 1480 | Macro 2PROSITE-ProRule annotationAdd BLAST | 127 | |
Domaini | 1480 – 1553 | DPUPPROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 1558 – 1613 | Ubiquitin-like 2PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 1628 – 1902 | Peptidase C16PROSITE-ProRule annotationAdd BLAST | 275 | |
Domaini | 1916 – 2033 | Nucleic acid-bindingPROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 2059 – 2179 | G2MPROSITE-ProRule annotationAdd BLAST | 121 | |
Domaini | 2725 – 2828 | CoV Nsp3 Y3PROSITE-ProRule annotationAdd BLAST | 104 | |
Domaini | 3242 – 3338 | Nsp4CPROSITE-ProRule annotationAdd BLAST | 97 | |
Domaini | 3339 – 3644 | Peptidase C30PROSITE-ProRule annotationAdd BLAST | 306 | |
Domaini | 3937 – 4019 | RdRp Nsp7 cofactorPROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 4020 – 4218 | RdRp Nsp8 cofactorPROSITE-ProRule annotationAdd BLAST | 199 | |
Domaini | 4219 – 4328 | Nsp9 ssRNA-bindingPROSITE-ProRule annotationAdd BLAST | 110 | |
Domaini | 4329 – 4467 | ExoN/MTase coactivatorPROSITE-ProRule annotationAdd BLAST | 139 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 340 – 361 | C4PROSITE-ProRule annotationAdd BLAST | 22 | |
Regioni | 2158 – 2441 | HD1By similarityAdd BLAST | 284 | |
Regioni | 2848 – 3223 | HD2By similarityAdd BLAST | 376 | |
Regioni | 3650 – 3875 | HD3By similarityAdd BLAST | 226 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1748 – 1785 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 4402 – 4418 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4444 – 4457 | By similarityAdd BLAST | 14 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
CDDi | cd21560, betaCoV-Nsp6, 1 hit cd21666, betaCoV_Nsp5_Mpro, 1 hit cd21517, cv_beta_Nsp2_MERS-like, 1 hit cd21473, cv_Nsp4_TM, 1 hit cd21563, Macro_cv_SUD-M_Nsp3-like, 1 hit cd21557, Macro_X_Nsp3-like, 1 hit cd21523, SUD_C_MERS-CoV_Nsp3, 1 hit cd21467, Ubl1_cv_Nsp3_N-like, 1 hit cd21466, Ubl2_cv_PLpro_N_Nsp3-like, 1 hit |
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.60.120.1680, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.30.70.3540, 1 hit 3.40.220.10, 1 hit 3.40.220.20, 1 hit 3.40.50.11020, 1 hit |
InterProi | View protein in InterPro IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR022733, DPUP_SUD_C_bCoV IPR002589, Macro_dom IPR043472, Macro_dom-like IPR044371, Macro_X_NSP3-like IPR042570, NAR_sf IPR036333, NSP10_sf_CoV IPR021590, NSP1_bCoV IPR044388, NSP2_MERS-like IPR043615, NSP2_N_CoV IPR024375, NSP3_bCoV IPR032592, NSP3_NAB_bCoV IPR038400, NSP3_SUD-M_sf_bCoV IPR044382, NSP3_SUD_C_MERS-CoV IPR044357, NSP3_Ubl1_dom_CoV IPR044353, Nsp3_Ubl2_dom_CoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR044367, NSP6_betaCoV IPR043610, NSP6_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF11501, bCoV_NSP1, 1 hit PF11633, bCoV_SUD_M, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51963, BCOV_NSP1_C, 1 hit PS51942, BCOV_NSP3C_C, 1 hit PS51941, BCOV_NSP3C_M, 1 hit PS51994, BCOV_NSP3E_G2M, 1 hit PS51945, BCOV_NSP3E_NAB, 1 hit PS51952, COV_EXON_MTASE_COACT, 1 hit PS51962, COV_NSP1, 1 hit PS51991, COV_NSP2_C, 1 hit PS51990, COV_NSP2_M, 1 hit PS51989, COV_NSP2_N, 1 hit PS51992, COV_NSP3_Y3, 1 hit PS51943, COV_NSP3A_UBL, 1 hit PS51944, COV_NSP3D_UBL, 1 hit PS51946, COV_NSP4C, 1 hit PS51949, COV_NSP7, 1 hit PS51950, COV_NSP8, 1 hit PS51951, COV_NSP9_SSRNA_BD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSFVAGVAPQ GARGKYRAEL NTEKRTDHVS LKASLCDAGD LVLKISPWFM
60 70 80 90 100
DGESAYKHVS EQLSKGSKLL FVPQTLKGFI RHLPGPRVYL VERLTGGTYS
110 120 130 140 150
DPFMVNQLAY QNAAGEGVIG TTLQGKRVGM FFPFDADLVT GEFQFLLRKK
160 170 180 190 200
GFGGNRFRDA PWDYNWTPYS DLMDALEADP CGKYSQSLLK KLVGGDFTPI
210 220 230 240 250
DQYMCGKNGK PIAEFAALMA SEGITKLADV EAEVKSRTDS DRYIVFKNKL
260 270 280 290 300
YRIVWNVQRK DVAYSKQSAF TMNSIVQLDT MEDVPRHSFT IGSEIQVIAP
310 320 330 340 350
STAVQANGHL NLKQRLLYAF YGKQAVSEPN YIYHSAYVDC TSCGKGSWLT
360 370 380 390 400
GNAVQGFACD CGAHYCANDV DLQSSGLVRK NAVLLTTCPC NKDGECKHTL
410 420 430 440 450
PQLVSMMTDK CDVEVVGKTF ILTYGGVIYA YMGCSGGTMH FIPRAKSCVS
460 470 480 490 500
KIGDAIFTGC TGTWSKVCET ANLFLERAQH AINFVNEFVL TETVVALLSG
510 520 530 540 550
TTSSIEELRD LCRNATFEKV RDYLTPRGWI VTMGSYIEGV INVGAAGVCN
560 570 580 590 600
AALNAPFIVL SGLGESFKKV AATPWKLCSS LRETLDHYAD SITYRVFPYD
610 620 630 640 650
IPCDVTDYTA LLLDCAVLTG ASAYFVARYV DEKVEQLTNL VFSSCQSAVA
660 670 680 690 700
AFVQACMSTY KATAKFISDM FTLIKVVSER LYVYTSVGFV VVGDYSSQLL
710 720 730 740 750
KQFMHILSKA MQLLHTTVSW AGSKLPSVVY NGRDSLVFPS GTYYCVSTQG
760 770 780 790 800
RSLQDQFDLV IPGDLSKKQI GILEPTPNST TVDKKINTNV VEVVVGQLEP
810 820 830 840 850
TKEHSPELVV GDYVIISNKI FVRSVEDSET VFYPLCTDGK IVPTLFRLKG
860 870 880 890 900
GAPPKGVKFG GEQTKEITAV RSVSVDYDVH PVLDALLAGS ELATFTVEKD
910 920 930 940 950
LPVKDFVDVV KDEVIELLSK LLRGYNVDGF DLEDFADTPC YVYNAEGDLA
960 970 980 990 1000
WSSTMTFSVN PVEEVEEECD DDYVEDEYLS EEMLVEEDEN SWAAAVEAVI
1010 1020 1030 1040 1050
PMEDVQLDTL VAEIDVSEPA DDVAEQASTE EVEVPSACVL EASQVANAAE
1060 1070 1080 1090 1100
VESCEAEVSS SIPLHEDANA AKANDCAEGM PALDSTETVS KLSVDTPVGD
1110 1120 1130 1140 1150
VTQDDATSSN ATVISEDVHT ATHSKGLVAV PEVVPEKALG TSVERMRSTS
1160 1170 1180 1190 1200
EWTVVETSLK QETAVIVKND SSAKPQRVKK PKAENPLKNF KHIVLNNDVT
1210 1220 1230 1240 1250
LVFGDAIAVA RATEDCILVN AANTHLKHGG GIAAAIDRAS GGLVQAESDD
1260 1270 1280 1290 1300
YVNFYGPLNV GDSTLLKGHG LATGILHVVG PDARANQDIQ LLKRCYKAFN
1310 1320 1330 1340 1350
KYPLVVSPLI SAGIFCVEPR VSLEYLLSVV HTKTYVVVNS EKVYNDLAAP
1360 1370 1380 1390 1400
KPPTGLTYSH EGWRGIIRNA KSFGFTCFIC TDQSANAKLL KGRGVDLTKK
1410 1420 1430 1440 1450
TQTVDGVKYY LYSSKDPLTD IITAANACKG ICAMPIGYVT HGLDLAQAGQ
1460 1470 1480 1490 1500
QVKKITVPYV CLLASKDQVP ILNSDVAVQT PEQSFINTVI ANGGYHCWHL
1510 1520 1530 1540 1550
VTGELIVKGV SYRKLLNWSD QTICYADNKF YVVKGQIALP FDSLEKCRTY
1560 1570 1580 1590 1600
LTSRAAQQKN VDVLVTIDGV NFRTVVLNNT TTYRVQLGSV FYKGSDISDT
1610 1620 1630 1640 1650
IPTEKMSGEA VYLADNLSEA EKAVLSEVYG TADTAFLHRY YSLLALVKKW
1660 1670 1680 1690 1700
KYTVHDGVKS LKLNSNNCYV NVTMLMLDML KEIKFIVPAL QAAYLKHKGG
1710 1720 1730 1740 1750
DSTEFIALIM AYGDCTYGEP DDASRLLHTI LSKAELTTQA KMVWRQWCNV
1760 1770 1780 1790 1800
CGVQDTTTTG LKACIYVGMN SLDELHATHE ECCQCGDVRK RQLVEHNAPW
1810 1820 1830 1840 1850
LLLSGLNEAK VMTPTSQSAG PDYTAFNVFQ GVETSVGHYL HVRVKDNLLY
1860 1870 1880 1890 1900
KYDSGSLSKT SDMKCKMTDV YYPKQRYSAD CNVVVYSLDG NTWADVDPDL
1910 1920 1930 1940 1950
SAFYMKDGKY FTKKPVIEYS PATILSGSVY TNSCLVGHDG TIGSDAISSS
1960 1970 1980 1990 2000
FNNLLGFDNS KPVSKKLTYS FFPDFEGDVI LTEYSTYDPI YKNGAMLHGK
2010 2020 2030 2040 2050
PILWVNNSKF DSALNKFNRA TLRQVYDIAP VTLENKYTVL QDNQIQQVEV
2060 2070 2080 2090 2100
EAPKEDAKPQ SPVQVAEDID NKLPIIKCKG LKKPFVKDGY SFVNDPQGVN
2110 2120 2130 2140 2150
VIDTLGIDDL RALYVDRNLR LIVLKENNWS ALFNIHTVEK GDLSVIAASG
2160 2170 2180 2190 2200
SITRRVKILL GASSLFAQFA SVTVNVTTAM GKALGRMTRN VITNTGIIGQ
2210 2220 2230 2240 2250
GFALLKMLLI LPFTFWKSKN QSTVKVEVGA LRTAGIVTTN VVKQCASAAY
2260 2270 2280 2290 2300
DVLVVKFKRI DWKSTLRLLF LICTTGLLLS SLYYLFLFHQ VLTSDVMLDG
2310 2320 2330 2340 2350
AEGMLATYRE LRSYLGIHSL CDGMVEAYRN VSYDVNDFCS NRSALCNWCL
2360 2370 2380 2390 2400
IGQDSLTRYS AFQMIQTHVT SYVINIDWVW FVMEFALAYV LYTSTFNVLL
2410 2420 2430 2440 2450
LVVSSQYFFS YTGAFVNWRS YNYLVSGYFF CVTHIPLLGL VRIYNFLACL
2460 2470 2480 2490 2500
WFLRRFYNHV INGCKDTACL LCYKRNRLTR VEASTVVCGS KRTFYIVANG
2510 2520 2530 2540 2550
GTSFCCRHNW NCVDCDTAGI GNTFICEEVA NDLTTSLRRL VKPTDKSHYY
2560 2570 2580 2590 2600
VESVTVKDSV VQLHYSREGA SCYERYPLCY FTNLDKLKFK EVCKTPTGIP
2610 2620 2630 2640 2650
EHNFLIYDSS DRGQENLARS ACVYYSQVLS KPMLLVDSNM VTTVGDSREI
2660 2670 2680 2690 2700
ASKMLDSYVN SFISLFGVNR DKLDKLVATA RDCVKRGDDF QTVIKTFTDA
2710 2720 2730 2740 2750
ARGPAGVESD VETSSIVDAL QYAYKHDLQL TTEGFNNYVP SYIKPDSVAT
2760 2770 2780 2790 2800
ADLGCLIDLN AASVNQTSIR NANGACIWNS SDYMKLSDSL KRQIRIACRK
2810 2820 2830 2840 2850
CNIPFRLTTS RLRSADNILS VKFSATKLSG GAPKWLLKLR DFTWKSYCVV
2860 2870 2880 2890 2900
TLVVFAMAVL SYLCLPAFNM SQVSFHEDRI LTYKVVENGI IRDITPSDTC
2910 2920 2930 2940 2950
FANKYQSFSK WFNEHYGGLF NNDISCPVTV AVIAGVAGAR VPNLPANVAW
2960 2970 2980 2990 3000
VGRQIVLFVS RVFASSNNVC YTPTAEIPYE RFSDSGCVLA SECTLFRDAE
3010 3020 3030 3040 3050
GKINPYCYDP TVLPGASAYD QMKPHVRYDM YDSDMYIKFP EVVFESTLRI
3060 3070 3080 3090 3100
TKTLATRYCR FGSCEDANEG VCITTNGSWA IYNDHYANKP GVYCGDNYFD
3110 3120 3130 3140 3150
IVRRLGLSLF QPVTYFQLST SLALGVMLCI FLTIAFYYVN KVKRALADYT
3160 3170 3180 3190 3200
QCAVVAVAAA LLNSLCLCFV VSNPLLVLPY TALYYYATFY LTGEPAFVMH
3210 3220 3230 3240 3250
VSWFVMFGTV VPIWMVFAYI VGVCLRHLLW VMAYFSKKHV EVFTDGKLNC
3260 3270 3280 3290 3300
SFQDAAANIF VINKDTYVAL RNSITQDSYN RYLSMFNKYK YYSGAMDTAS
3310 3320 3330 3340 3350
YREASAAHLC KALQVYSETG SDVLFQPPNC SVTSSVLQSG LVKMAAPSGV
3360 3370 3380 3390 3400
VENCMVQVTC GSMTLNGLWL DNYVWCPRHV MCPADQLSDP NYDALLVSKT
3410 3420 3430 3440 3450
NLSFIVQKNV GAPANLRVVG HTMVGTLLKL TVESANPQTP AYTFTTVKPG
3460 3470 3480 3490 3500
ASFSVLACYN GRPTGVFMVN MRQNSTIKGS FLCGSCGSVG YTQEGNVINF
3510 3520 3530 3540 3550
CYMHQMELSN GTHTGCAFDG VMYGAFEDRQ VHQVQLSDKY CTINIVAWLY
3560 3570 3580 3590 3600
AAILNGCNWF VKPNKTGIAT FNEWAMSNQF TEFIGTQSVD MLAHKTGVSV
3610 3620 3630 3640 3650
EQLLYAIQTL HKGFQGKTIL GNSMLEDEFT PDDVNMQVMG VVMQSGVKRI
3660 3670 3680 3690 3700
SYGLVHWLFT TLLLAYVATL QLTKFTIWNY LFEVIPLQLT PLVLCVMACV
3710 3720 3730 3740 3750
MLTVKHKHTF LTLFLLPTAI CLTYANIVYE PQTPVSSALI AVANWLNPAS
3760 3770 3780 3790 3800
VYMRTTHTDL GVYLSLCFAL AVVVRRLYRP NASNLALALG SAMVWFYTYT
3810 3820 3830 3840 3850
TGDCSSPLTY LMFLTTLTSD YTVTVFLAVN VAKFFARVVF LYAPHAGFIF
3860 3870 3880 3890 3900
PEVKLVLLMY LAVGYFCTVY FGVFSLLNLK LRVPLGVYDY TVSTQEFRYL
3910 3920 3930 3940 3950
TGNGLHAPRN SWEALRLNMK LIGIGGTPCI KIASVQSKLT DLKCTSVVLL
3960 3970 3980 3990 4000
SVLQQLHLEA NSKAWAHCVK LHNDILAATD PTEAFDNFVC LFATLMSFSA
4010 4020 4030 4040 4050
NVDLEALASD LLDHPSVLQA TLSEFSHLAS YAELEAAQSS YQKALNSGDA
4060 4070 4080 4090 4100
SPQVLKALQK AVNIAKNAYE KDKAVARKLE RMAEQAMTSM YKQARAEDKK
4110 4120 4130 4140 4150
AKIVSAMQTM LFGMIKKLDN DVLNGVISNA RNGCVPLSVV PLCASNKLRV
4160 4170 4180 4190 4200
VIPDITIWNK VVTWPSLSYA GALWDISLIN NVDGEVVKSS DVTETNESLT
4210 4220 4230 4240 4250
WPLVLECTRA ASSAVTLQNN EIRPSGLKTM VVSAGIDHAN CNTSSLAYYE
4260 4270 4280 4290 4300
PVEGRKMLMG ILSENAHLKW AKVEGRDGFV NIELQPPCKF LIAGPKGPEV
4310 4320 4330 4340 4350
RYLYFVKNLN NLHRGQLLGH IAATVRLQAG SNTEFAINSS VLSAVTFSVD
4360 4370 4380 4390 4400
PGKAYLDFVN AGGAPLTNCV KMLTPKTGTG IAVSVKPEAN ADQDTYGGAS
4410 4420 4430 4440 4450
VCLYCRAHIE HPDVTGVCKF KGKFVQVPLH IRDPVGFCLQ NTPCNVCQFW
4460 4470 4480
IGHGCNCDAL RGTTIPQSKD SNFLNESGVL L
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF065509 Genomic RNA No translation available. |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF065509 Genomic RNA No translation available. |
3D structure databases
SMRi | P0C6T5 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P0C6T5, 1 interactor |
Enzyme and pathway databases
SABIO-RKi | P0C6T5 |
Family and domain databases
CDDi | cd21560, betaCoV-Nsp6, 1 hit cd21666, betaCoV_Nsp5_Mpro, 1 hit cd21517, cv_beta_Nsp2_MERS-like, 1 hit cd21473, cv_Nsp4_TM, 1 hit cd21563, Macro_cv_SUD-M_Nsp3-like, 1 hit cd21557, Macro_X_Nsp3-like, 1 hit cd21523, SUD_C_MERS-CoV_Nsp3, 1 hit cd21467, Ubl1_cv_Nsp3_N-like, 1 hit cd21466, Ubl2_cv_PLpro_N_Nsp3-like, 1 hit |
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.60.120.1680, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.30.70.3540, 1 hit 3.40.220.10, 1 hit 3.40.220.20, 1 hit 3.40.50.11020, 1 hit |
InterProi | View protein in InterPro IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR022733, DPUP_SUD_C_bCoV IPR002589, Macro_dom IPR043472, Macro_dom-like IPR044371, Macro_X_NSP3-like IPR042570, NAR_sf IPR036333, NSP10_sf_CoV IPR021590, NSP1_bCoV IPR044388, NSP2_MERS-like IPR043615, NSP2_N_CoV IPR024375, NSP3_bCoV IPR032592, NSP3_NAB_bCoV IPR038400, NSP3_SUD-M_sf_bCoV IPR044382, NSP3_SUD_C_MERS-CoV IPR044357, NSP3_Ubl1_dom_CoV IPR044353, Nsp3_Ubl2_dom_CoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR044367, NSP6_betaCoV IPR043610, NSP6_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF11501, bCoV_NSP1, 1 hit PF11633, bCoV_SUD_M, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51963, BCOV_NSP1_C, 1 hit PS51942, BCOV_NSP3C_C, 1 hit PS51941, BCOV_NSP3C_M, 1 hit PS51994, BCOV_NSP3E_G2M, 1 hit PS51945, BCOV_NSP3E_NAB, 1 hit PS51952, COV_EXON_MTASE_COACT, 1 hit PS51962, COV_NSP1, 1 hit PS51991, COV_NSP2_C, 1 hit PS51990, COV_NSP2_M, 1 hit PS51989, COV_NSP2_N, 1 hit PS51992, COV_NSP3_Y3, 1 hit PS51943, COV_NSP3A_UBL, 1 hit PS51944, COV_NSP3D_UBL, 1 hit PS51946, COV_NSP4C, 1 hit PS51949, COV_NSP7, 1 hit PS51950, COV_NSP8, 1 hit PS51951, COV_NSP9_SSRNA_BD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | R1A_BCHK5 | |
Accessioni | P0C6T5Primary (citable) accession number: P0C6T5 Secondary accession number(s): A3EXC9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | May 25, 2022 | |
This is version 90 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families