UniProtKB - P0C6T4 (R1A_BCHK4)
Replicase polyprotein 1a
1a
Functioni
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).
By similarityResponsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
PROSITE-ProRule annotationNsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
By similarityNsp9 is a ssRNA-binding protein.
By similarityBinds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).
By similarityCatalytic activityi
- TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. EC:3.4.22.69
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1634 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1800 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3332 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3439 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Metal bindingi | 4355 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4358 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4364 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4371 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4397 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4400 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4408 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 4410 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1714 – 1751 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 4355 – 4371 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4397 – 4410 | By similarityAdd BLAST | 14 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: InterPro
- G-quadruplex RNA binding Source: InterPro
- single-stranded RNA binding Source: InterPro
- thiol-dependent deubiquitinase Source: UniProtKB-EC
- transferase activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- induction by virus of catabolism of host mRNA Source: UniProtKB-KW
- induction by virus of host autophagy Source: UniProtKB-KW
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- suppression by virus of host gene expression Source: UniProtKB-KW
- suppression by virus of host ISG15-protein conjugation Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
- suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity Source: UniProtKB-KW
- viral genome replication Source: InterPro
- viral protein processing Source: InterPro
Keywordsi
Enzyme and pathway databases
SABIO-RKi | P0C6T4 |
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1aShort name: pp1a Alternative name(s): ORF1a polyprotein Cleaved into the following 11 chains: Alternative name(s): Leader protein Alternative name(s): p65 homolog Alternative name(s): PL2-PRO Papain-like proteinase Short name: PL-PRO Alternative name(s): nsp5 Alternative name(s): Growth factor-like peptide Short name: GFL |
Gene namesi | ORF Names:1a |
Organismi | Bat coronavirus HKU4 (BtCoV) (BtCoV/HKU4/2004) |
Taxonomic identifieri | 694007 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Merbecovirus |
Virus hosti | Tylonycteris pachypus (Lesser bamboo bat) (Vespertilio pachypus) [TaxID: 258959] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 2145 – 2165 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2222 – 2242 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2326 – 2346 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2350 – 2370 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2375 – 2395 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2800 – 2820 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3072 – 3092 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3105 – 3125 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3149 – 3169 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3603 – 3623 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3637 – 3657 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3662 – 3682 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3707 – 3727 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3735 – 3755 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3784 – 3804 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3808 – 3828 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000338086 | 1 – 4434 | Replicase polyprotein 1aAdd BLAST | 4434 | |
ChainiPRO_0000338087 | 1 – 195 | Non-structural protein 1Sequence analysisAdd BLAST | 195 | |
ChainiPRO_0000338088 | 196 – 847 | Non-structural protein 2Sequence analysisAdd BLAST | 652 | |
ChainiPRO_0000338089 | 848 – 2784 | Non-structural protein 3Sequence analysisAdd BLAST | 1937 | |
ChainiPRO_0000338090 | 2785 – 3291 | Non-structural protein 4Sequence analysisAdd BLAST | 507 | |
ChainiPRO_0000338091 | 3292 – 3597 | 3C-like proteinaseSequence analysisAdd BLAST | 306 | |
ChainiPRO_0000338092 | 3598 – 3889 | Non-structural protein 6Sequence analysisAdd BLAST | 292 | |
ChainiPRO_0000338093 | 3890 – 3972 | Non-structural protein 7Sequence analysisAdd BLAST | 83 | |
ChainiPRO_0000338094 | 3973 – 4171 | Non-structural protein 8Sequence analysisAdd BLAST | 199 | |
ChainiPRO_0000338095 | 4172 – 4281 | Non-structural protein 9Sequence analysisAdd BLAST | 110 | |
ChainiPRO_0000338097 | 4281 – 4434 | Non-structural protein 11Sequence analysisAdd BLAST | 154 | |
ChainiPRO_0000338096 | 4282 – 4420 | Non-structural protein 10Sequence analysisAdd BLAST | 139 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 195 – 196 | CleavageSequence analysis | 2 | |
Sitei | 847 – 848 | Cleavage; by PL-PROSequence analysis | 2 | |
Sitei | 2784 – 2785 | Cleavage; by PL-PROSequence analysis | 2 | |
Sitei | 3291 – 3292 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 3597 – 3598 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 3889 – 3890 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 3972 – 3973 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 4171 – 4172 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 4281 – 4282 | Cleavage; by 3CL-PROSequence analysis | 2 | |
Sitei | 4420 – 4421 | Cleavage; by 3CL-PROSequence analysis | 2 |
Interactioni
Subunit structurei
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
By similarityStructurei
Secondary structure
3D structure databases
SMRi | P0C6T4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 154 | CoV Nsp1 globularPROSITE-ProRule annotationAdd BLAST | 130 | |
Domaini | 167 – 195 | BetaCoV Nsp1 C-terminalPROSITE-ProRule annotationAdd BLAST | 29 | |
Domaini | 850 – 960 | Ubiquitin-like 1PROSITE-ProRule annotationAdd BLAST | 111 | |
Domaini | 1152 – 1321 | Macro 1PROSITE-ProRule annotationAdd BLAST | 170 | |
Domaini | 1322 – 1446 | Macro 2PROSITE-ProRule annotationAdd BLAST | 125 | |
Domaini | 1446 – 1519 | DPUPPROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 1524 – 1579 | Ubiquitin-like 2PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 1593 – 1864 | Peptidase C16PROSITE-ProRule annotationAdd BLAST | 272 | |
Domaini | 1878 – 1995 | Nucleic acid-bindingPROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 3195 – 3291 | Nsp4CPROSITE-ProRule annotationAdd BLAST | 97 | |
Domaini | 3292 – 3597 | Peptidase C30PROSITE-ProRule annotationAdd BLAST | 306 | |
Domaini | 3890 – 3972 | RdRp Nsp7 cofactorPROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 3973 – 4171 | RdRp Nsp8 cofactorPROSITE-ProRule annotationAdd BLAST | 199 | |
Domaini | 4172 – 4281 | Nsp9 ssRNA-bindingPROSITE-ProRule annotationAdd BLAST | 110 | |
Domaini | 4282 – 4420 | ExoN/MTase coactivatorPROSITE-ProRule annotationAdd BLAST | 139 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2112 – 2395 | HD1By similarityAdd BLAST | 284 | |
Regioni | 2800 – 3169 | HD2By similarityAdd BLAST | 370 | |
Regioni | 3603 – 3828 | HD3By similarityAdd BLAST | 226 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1714 – 1751 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 4355 – 4371 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4397 – 4410 | By similarityAdd BLAST | 14 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
CDDi | cd21560, betaCoV-Nsp6, 1 hit cd21666, betaCoV_Nsp5_Mpro, 1 hit cd21473, cv_Nsp4_TM, 1 hit cd21563, Macro_cv_SUD-M_Nsp3-like, 1 hit cd21557, Macro_X_Nsp3-like, 1 hit cd21523, SUD_C_MERS-CoV_Nsp3, 1 hit cd21467, Ubl1_cv_Nsp3_N-like, 1 hit cd21466, Ubl2_cv_PLpro_N_Nsp3-like, 1 hit |
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.60.120.1680, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.30.70.3540, 1 hit 3.40.220.10, 1 hit 3.40.220.20, 1 hit 3.40.50.11020, 1 hit |
InterProi | View protein in InterPro IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR022733, DPUP_SUD_C_bCoV IPR002589, Macro_dom IPR043472, Macro_dom-like IPR044371, Macro_X_NSP3-like IPR042570, NAR_sf IPR036333, NSP10_sf_CoV IPR021590, NSP1_bCoV IPR043615, NSP2_N_CoV IPR024375, NSP3_bCoV IPR032592, NSP3_NAB_bCoV IPR038400, NSP3_SUD-M_sf_bCoV IPR044382, NSP3_SUD_C_MERS-CoV IPR044357, NSP3_Ubl1_dom_CoV IPR044353, Nsp3_Ubl2_dom_CoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR044367, NSP6_betaCoV IPR043610, NSP6_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF11501, bCoV_NSP1, 1 hit PF11633, bCoV_SUD_M, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51963, BCOV_NSP1_C, 1 hit PS51942, BCOV_NSP3C_C, 1 hit PS51941, BCOV_NSP3C_M, 1 hit PS51945, BCOV_NSP3E_NAB, 1 hit PS51952, COV_EXON_MTASE_COACT, 1 hit PS51962, COV_NSP1, 1 hit PS51943, COV_NSP3A_UBL, 1 hit PS51944, COV_NSP3D_UBL, 1 hit PS51946, COV_NSP4C, 1 hit PS51949, COV_NSP7, 1 hit PS51950, COV_NSP8, 1 hit PS51951, COV_NSP9_SSRNA_BD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLSKASVTTQ GARGKYRAEL YNEKRSDHVA CTVPLCDTDD MACKLTPWFE
60 70 80 90 100
DGETAFNQVS SILKEKGKIL FVPMHMQRAM KFLPGPRVYL VERLTGGMLS
110 120 130 140 150
KHFLVNQLAY KDQVGAAMMR TTLNAKPLGM FFPYDSSLET GEYTFLLRKN
160 170 180 190 200
GLGGQLFRER PWDRKETPYV EILDDLEADP TGKYSQNLLK KLIGGDCIPI
210 220 230 240 250
DQYMCGKNGK PIADYAKIVA KEGLTTLADI EVDVKSRMDS DRFIVLNKKL
260 270 280 290 300
YRVVWNVTRR NVPYPKQTAF TIVSVVQCDD KDSVPEHTFT IGSQILMVSP
310 320 330 340 350
LKATNNKNFN LKQRLLYTFY GKDAVQQPGY IYHSAYVDCN ACGRGTWCTG
360 370 380 390 400
NAIQGFACDC GANYSANDVD LQSSGLVPRN ALFLANCPCA NNGACSHSAA
410 420 430 440 450
QVYNILDGKA CVEVGGKSFT LTFGGVVYAY MGCCDGTMYF VPRAKSCVSR
460 470 480 490 500
IGDAIFTGCT GTWDKVVETA NLFLEKAQRS LNFCQQFALT EVVLAILSGT
510 520 530 540 550
TSTFEELRDL CHNASYEKVR DHLVNHGFVV TIGDYIRDAI NIGANGVCNA
560 570 580 590 600
TINAPFIAFT GLGESFKKVS AIPWKICSNL KSALDYYSSN IMFRVFPYDI
610 620 630 640 650
PCDVSNFVEL LLDCGKLTVA TSYFVLRYLD EKFDTVLGTV SSACQTALSS
660 670 680 690 700
FLNACVAASR ATAGFINDMF KLFKVLMHKL YVYTSCGYVA VAEHSSKIVQ
710 720 730 740 750
QVLDIMSKAM KLLHTNVSWA GTKLSAIIYE GREALLFNSG TYFCLSTKAK
760 770 780 790 800
TLQGQMNLVL PGDYNKKTLG ILDPVPNADT IDVNANSTVV DVVHGQLEPT
810 820 830 840 850
NEHGPSMIVG NYVLVSDKLF VRTEDEEFYP LCTNGKVVST LFRLKGGMPS
860 870 880 890 900
KKVTFGDVNT VEVTAYRSVS ITYDIHPVLD ALLSSSKLAT FTVEKDLLVE
910 920 930 940 950
DFVDVIKDEV LTLLTPLLRG YDIDGFDVED FIDVPCYVYN QDGDCAWSSN
960 970 980 990 1000
MTFSINPVED VEEVEEFIED DYLSDELPIA DDEEAWARAV EEVMPLDDIL
1010 1020 1030 1040 1050
VAEIELEEDP PLETALESVE AEVVETAEAQ EPSVESIDST PSTSTVVGEN
1060 1070 1080 1090 1100
DLSVKPMSRV AETDDVLELE TAVVGGPVSD VTAIVTNDIV SVEQAQQCGV
1110 1120 1130 1140 1150
SSLPIQDEAS ENQVHQVSDL QGNELLCSET KVEIVQPRQD LKPRRSRKSK
1160 1170 1180 1190 1200
VDLSKYKHTV INNSVTLVLG DAIQIASLLP KCILVNAANR HLKHGGGIAG
1210 1220 1230 1240 1250
VINKASGGDV QEESDEYISN NGPLHVGDSV LLKGHGLADA ILHVVGPDAR
1260 1270 1280 1290 1300
NNEDAALLKR CYKAFNKHTI VVTPLISAGI FSVDPKVSFE YLLANVTTTT
1310 1320 1330 1340 1350
YVVVNNEDIY NTLATPSKPD GLVYSFEGWR GTVRTAKNYG FTCFICTEYS
1360 1370 1380 1390 1400
ANVKFLRTKG VDTTKKIQTV DGVSYYLYSA RDALTDVIAA ANGCSGICAM
1410 1420 1430 1440 1450
PFGYVTHGLD LAQSGNYVRQ VKVPYVCLLA SKEQIPIMNS DVAIQTPETA
1460 1470 1480 1490 1500
FINNVTSNGG YHSWHLVSGD LIVKDVCYKK LLHWSGQTIC YADNKFYVVK
1510 1520 1530 1540 1550
NDVALPFSDL EACRAYLTSR AAQQVNIEVL VTIDGVNFRT VILNDTTTFR
1560 1570 1580 1590 1600
KQLGATFYKG VDISDAFPTV KMGGESLFVA DNLSESEKVV LKEYYGTSDV
1610 1620 1630 1640 1650
TFLQRYYSLQ PLVQQWKFVV HDGVKSLKLS NYNCYINATI MMIDMLHDIK
1660 1670 1680 1690 1700
FVVPALQNAY LRYKGGDPYD FLALIMAYGD CTFDNPDDEA KLLHTLLAKA
1710 1720 1730 1740 1750
ELTVSAKMVW REWCTVCGIR DIEYTGMRAC VYAGVNSMEE LQSVFNETCV
1760 1770 1780 1790 1800
CGSVKHRQLV EHSAPWLLVS GLNEVKVSTS TDPIYRAFNV FQGVETSVGH
1810 1820 1830 1840 1850
YVHIRVKDGL FYKYDSGSLT KTSDMKCKMT SVWYPTVRYT ADCNVVVYDL
1860 1870 1880 1890 1900
DGVTKVEVNP DLSNYYMKDG KYYTSKPTIK YSPATILPGS VYSNSCLVGV
1910 1920 1930 1940 1950
DGTPGSDTIS KFFNDLLGFD ETKPISKKLT YSLLPNEDGD VLLSEFSNYN
1960 1970 1980 1990 2000
PVYKKGVMLK GKPILWVNNG VCDSALNKPN RASLRQLYDV APIVLDNKYT
2010 2020 2030 2040 2050
VLQDNTSQLV EHNVPVVDDV PITTRKLIEV KCKGLNKPFV KGNFSFVNDP
2060 2070 2080 2090 2100
NGVTVVDTLG LTELRALYVD INTRYIVLRD NNWSSLFKLH TVESGDLQIV
2110 2120 2130 2140 2150
AAGGSVTRRA RVLLGASSLF ASFAKITVTA TTAACKTAGR GFCKFVVNYG
2160 2170 2180 2190 2200
VLQNMFVFLK MLFFLPFNYL WPKKQPTVDI GVSGLRTAGI VTTNIVKQCG
2210 2220 2230 2240 2250
TAAYYMLLGK FKRVDWKATL RLFLLLCTTI LLLSSIYHLV LFNQVLSSDV
2260 2270 2280 2290 2300
MLEDATGILA IYKEVRSYLG IRTLCDGLVV EYRNTSFDVM EFCSNRSVLC
2310 2320 2330 2340 2350
QWCLIGQDSL TRYSALQMLQ THITSYVLNI DWIWFALEFF LAYVLYTSSF
2360 2370 2380 2390 2400
NVLLLVVTAQ YFFAYTSAFV NWRAYNYIVS GLFFLVTHIP LHGLVRVYNF
2410 2420 2430 2440 2450
LACLWFLRKF YSHVINGCKD TACLLCYKRN RLTRVEASTI VCGTKRTFYI
2460 2470 2480 2490 2500
AANGGTSYCC KHNWNCVECD TAGVGNTFIC TEVANDLTTT LRRLIKPTDQ
2510 2520 2530 2540 2550
SHYYVDSVVV KDAVVELHYN RDGSSCYERY PLCYFTNLEK LKFKEVCKTP
2560 2570 2580 2590 2600
TGIPEHNFLI YDTNDRGQEN LARSACVYYS QVLCKPMLLV DVNLVTTVGD
2610 2620 2630 2640 2650
SREIAIKMLD SFINSFISLF SVSRDKLEKL INTARDCVRR GDDFQNVLKT
2660 2670 2680 2690 2700
FTDAARGHAG VESDVETTMV VDALQYAHKN DIQLTTECYN NYVPGYIKPD
2710 2720 2730 2740 2750
SINTLDLGCL IDLKAASVNQ TSMRNANGAC VWNSGDYMKL SDSFKRQIRI
2760 2770 2780 2790 2800
ACRKCNIPFR LTTSKLRAAD NILSVKFSAT KIVGGAPSWL LRVRDLTVKG
2810 2820 2830 2840 2850
YCILTLFVFT VAVLSWFCLP SYSIATVNFN DDRILTYKVI ENGIVRDIAP
2860 2870 2880 2890 2900
NDVCFANKYG HFSKWFNENH GGVYRNSMDC PITIAVIAGV AGARVANVPA
2910 2920 2930 2940 2950
NLAWVGKQIV LFVSRVFANT NVCFTPINEI PYDTFSDSGC VLSSECTLFR
2960 2970 2980 2990 3000
DAEGNLNPFC YDPTVLPGAS SYADMKPHVR YDMYDSDMYI KFPEVIVEST
3010 3020 3030 3040 3050
LRITKTLATQ YCRFGSCEES AAGVCISTNG SWALYNQNYS TRPGIYCGDD
3060 3070 3080 3090 3100
YFDIVRRLAI SLFQPVTYFQ LSTSLAMGLV LCVFLTAAFY YINKVKRALA
3110 3120 3130 3140 3150
DYTQCAVVAV VAALLNSLCL CFIVANPLLV APYTAMYYYA TFYLTGEPAF
3160 3170 3180 3190 3200
IMHISWYVMF GAVVPIWMLA SYTVGVMLRH LFWVLAYFSK KHVDVFTDGK
3210 3220 3230 3240 3250
LNCSFQDAAS NIFVIGKDTY VALRNAITQD SFVRYLSLFN KYKYYSGAMD
3260 3270 3280 3290 3300
TASYREACAA HLCKALQTYS ETGSDILYQP PNCSVTSSVL QSGLVKMSAP
3310 3320 3330 3340 3350
SGAVENCIVQ VTCGSMTLNG LWLDNTVWCP RHIMCPADQL TDPNYDALLI
3360 3370 3380 3390 3400
SKTNHSFIVQ KHIGAQANLR VVAHSMVGVL LKLTVDVANP STPAYTFSTV
3410 3420 3430 3440 3450
KPGASFSVLA CYNGKPTGVF TVNLRHNSTI KGSFLCGSCG SVGYTENGGV
3460 3470 3480 3490 3500
INFVYMHQME LSNGTHTGSS FDGVMYGAFE DKQTHQLQLT DKYCTINVVA
3510 3520 3530 3540 3550
WLYAAVLNGC KWFVKPTRVG IVTYNEWALS NQFTEFVGTQ SIDMLAHRTG
3560 3570 3580 3590 3600
VSVEQMLAAI QSLHAGFQGK TILGQSTLED EFTPDDVNMQ VMGVVMQSGV
3610 3620 3630 3640 3650
KRISYGFIHW LISTFVLAYV SVMQLTKFTM WTYLFETIPT QMTPLLLGFM
3660 3670 3680 3690 3700
ACVMFTVKHK HTFMSLFLLP VALCLTYANI VYEPQTLISS TLIAVANWLT
3710 3720 3730 3740 3750
PTSVYMRTTH FDFGLYISLS FVLAIIVRRL YRPSMSNLAL ALCSGVMWFY
3760 3770 3780 3790 3800
TYVIGDHSSP ITYLMFITTL TSDYTITVFA TVNLAKFISG LVFFYAPHLG
3810 3820 3830 3840 3850
FILPEVKLVL LIYLGLGYMC TMYFGVFSLL NLKLRVPLGV YDYSVSTQEF
3860 3870 3880 3890 3900
RFLTGNGLHA PRNSWEALIL NFKLLGIGGT PCIKVATVQS KLTDLKCTSV
3910 3920 3930 3940 3950
VLLTVLQQLH LESNSKAWSY CVKLHNEILA AVDPTEAFER FVCLFATLMS
3960 3970 3980 3990 4000
FSANVDLDAL ANDLFENSSV LQATLTEFSH LATYAELETA QSSYQKALNS
4010 4020 4030 4040 4050
GDASPQVLKA LQKAVNVAKN AYEKDKAVAR KLERMAEQAM TSMYKQARAE
4060 4070 4080 4090 4100
DKKAKIVSAM QTMLFGMIKK LDNDVLNGVI ANARNGCVPL SIVPLCASNK
4110 4120 4130 4140 4150
LRVVIPDISV WNKVVNWPSV SYAGSLWDIT VINNVDNEVV KPTDVVETNE
4160 4170 4180 4190 4200
SLTWPLVIEC SRSSSSAVKL QNNEIHPKGL KTMVITAGVD QVNCNSSAVA
4210 4220 4230 4240 4250
YYEPVQGHRM VMGLLSENAH LKWAKVEGKD GFINIELQPP CKFLIAGPKG
4260 4270 4280 4290 4300
PEIRYLYFVK NLNNLHRGQL LGHIAATVRL QAGANTEFAS NSTVLTLVAF
4310 4320 4330 4340 4350
AVDPAKAYLD YVGSGGTPLS NYVKMLAPKT GTGVAISVKP EATADQETYG
4360 4370 4380 4390 4400
GASVCLYCRA HIEHPDVSGV CKYKTRFVQI PAHVRDPVGF LLKNVPCNVC
4410 4420 4430
QYWVGYGCNC DALRNNTVPQ SKDTNFLNES GVLV
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF065505 Genomic RNA No translation available. |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF065505 Genomic RNA No translation available. |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YNA | X-ray | 1.50 | A/B | 3292-3597 | [»] | |
2YNB | X-ray | 1.96 | A/B | 3292-3597 | [»] | |
SMRi | P0C6T4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
SABIO-RKi | P0C6T4 |
Family and domain databases
CDDi | cd21560, betaCoV-Nsp6, 1 hit cd21666, betaCoV_Nsp5_Mpro, 1 hit cd21473, cv_Nsp4_TM, 1 hit cd21563, Macro_cv_SUD-M_Nsp3-like, 1 hit cd21557, Macro_X_Nsp3-like, 1 hit cd21523, SUD_C_MERS-CoV_Nsp3, 1 hit cd21467, Ubl1_cv_Nsp3_N-like, 1 hit cd21466, Ubl2_cv_PLpro_N_Nsp3-like, 1 hit |
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.60.120.1680, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.30.70.3540, 1 hit 3.40.220.10, 1 hit 3.40.220.20, 1 hit 3.40.50.11020, 1 hit |
InterProi | View protein in InterPro IPR043613, CoV_NSP2_C IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR022733, DPUP_SUD_C_bCoV IPR002589, Macro_dom IPR043472, Macro_dom-like IPR044371, Macro_X_NSP3-like IPR042570, NAR_sf IPR036333, NSP10_sf_CoV IPR021590, NSP1_bCoV IPR043615, NSP2_N_CoV IPR024375, NSP3_bCoV IPR032592, NSP3_NAB_bCoV IPR038400, NSP3_SUD-M_sf_bCoV IPR044382, NSP3_SUD_C_MERS-CoV IPR044357, NSP3_Ubl1_dom_CoV IPR044353, Nsp3_Ubl2_dom_CoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR044367, NSP6_betaCoV IPR043610, NSP6_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF11501, bCoV_NSP1, 1 hit PF11633, bCoV_SUD_M, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51963, BCOV_NSP1_C, 1 hit PS51942, BCOV_NSP3C_C, 1 hit PS51941, BCOV_NSP3C_M, 1 hit PS51945, BCOV_NSP3E_NAB, 1 hit PS51952, COV_EXON_MTASE_COACT, 1 hit PS51962, COV_NSP1, 1 hit PS51943, COV_NSP3A_UBL, 1 hit PS51944, COV_NSP3D_UBL, 1 hit PS51946, COV_NSP4C, 1 hit PS51949, COV_NSP7, 1 hit PS51950, COV_NSP8, 1 hit PS51951, COV_NSP9_SSRNA_BD, 1 hit PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | R1A_BCHK4 | |
Accessioni | P0C6T4Primary (citable) accession number: P0C6T4 Secondary accession number(s): A3EX93 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | February 23, 2022 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families