UniProtKB - P0C6T2 (OST4_HUMAN)
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4
OST4
Functioni
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667).
N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. Specifically involved in maintaining stability of STT3A-containing OST complexes.
2 Publications: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Biological processi
- protein N-linked glycosylation Source: ComplexPortal
- protein N-linked glycosylation via asparagine Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | P0C6T2 |
SignaLinki | P0C6T2 |
UniPathwayi | UPA00378 |
Protein family/group databases
TCDBi | 9.B.142.3.17, the integral membrane glycosyltransferase family 39 (gt39) family |
Names & Taxonomyi
Protein namesi | Recommended name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4Curated |
Gene namesi | Name:OST4Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:32483, OST4 |
MIMi | 618932, gene |
neXtProti | NX_P0C6T2 |
VEuPathDBi | HostDB:ENSG00000228474 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
- Endoplasmic reticulum membrane ; Single-pass type III membrane protein Curated
Note: The single transmembrane helix has a kink in the middle of the transmembrane span.
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: ComplexPortal
- oligosaccharyltransferase complex Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | LumenalSequence analysis | 4 | |
Transmembranei | 5 – 25 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 26 – 37 | CytoplasmicSequence analysisAdd BLAST | 12 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 | V → K: Decreases interaction with STT3A, STT3B and RPN1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 100128731 |
OpenTargetsi | ENSG00000228474 |
PharmGKBi | PA165697090 |
Miscellaneous databases
Pharosi | P0C6T2, Tdark |
Genetic variation databases
BioMutai | OST4 |
DMDMi | 182894143 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000328638 | 1 – 37 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4Add BLAST | 37 |
Proteomic databases
jPOSTi | P0C6T2 |
MassIVEi | P0C6T2 |
PaxDbi | P0C6T2 |
PeptideAtlasi | P0C6T2 |
PRIDEi | P0C6T2 |
TopDownProteomicsi | P0C6T2 |
Expressioni
Gene expression databases
Bgeei | ENSG00000228474, Expressed in prostate gland and 216 other tissues |
Genevisiblei | P0C6T2, HS |
Organism-specific databases
HPAi | ENSG00000228474, Low tissue specificity |
Interactioni
Subunit structurei
Component of the oligosaccharyltransferase (OST) complex (PubMed:31831667). OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits (PubMed:23606741, PubMed:31831667). STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).
By similarity2 PublicationsBinary interactionsi
P0C6T2
With | #Exp. | IntAct |
---|---|---|
MSGN1 [A6NI15] | 3 | EBI-18397963,EBI-11991020 |
UBQLN1 [Q9UMX0] | 3 | EBI-18397963,EBI-741480 |
Protein-protein interaction databases
BioGRIDi | 933663, 8 interactors |
ComplexPortali | CPX-5621, Oligosaccharyltransferase complex A CPX-5622, Oligosaccharyltransferase complex B |
IntActi | P0C6T2, 3 interactors |
STRINGi | 9606.ENSP00000457935 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P0C6T2 |
BMRBi | P0C6T2 |
SMRi | P0C6T2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502TACH, Eukaryota |
GeneTreei | ENSGT00390000016516 |
HOGENOMi | CLU_186352_2_0_1 |
InParanoidi | P0C6T2 |
OrthoDBi | 1584372at2759 |
PhylomeDBi | P0C6T2 |
Family and domain databases
InterProi | View protein in InterPro IPR018943, Oligosaccaryltransferase IPR036330, Ost4p_sf |
Pfami | View protein in Pfam PF10215, Ost4, 1 hit |
SUPFAMi | SSF103464, SSF103464, 1 hit |
i Sequence
Sequence statusi: Complete.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC013403 Genomic DNA No translation available. BC015653 mRNA No translation available. |
CCDSi | CCDS58703.1 |
RefSeqi | NP_001128165.1, NM_001134693.1 |
Genome annotation databases
Ensembli | ENST00000429985.1; ENSP00000455716.1; ENSG00000228474.6 ENST00000447619.5; ENSP00000454411.1; ENSG00000228474.6 ENST00000456793.2; ENSP00000457935.1; ENSG00000228474.6 |
GeneIDi | 100128731 |
KEGGi | hsa:100128731 |
MANE-Selecti | ENST00000456793.2; ENSP00000457935.1; NM_001134693.2; NP_001128165.1 |
UCSCi | uc002rig.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC013403 Genomic DNA No translation available. BC015653 mRNA No translation available. |
CCDSi | CCDS58703.1 |
RefSeqi | NP_001128165.1, NM_001134693.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LAT | NMR | - | A | 1-37 | [»] | |
6S7O | electron microscopy | 3.50 | B | 1-37 | [»] | |
6S7T | electron microscopy | 3.50 | B | 1-37 | [»] | |
AlphaFoldDBi | P0C6T2 | |||||
BMRBi | P0C6T2 | |||||
SMRi | P0C6T2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 933663, 8 interactors |
ComplexPortali | CPX-5621, Oligosaccharyltransferase complex A CPX-5622, Oligosaccharyltransferase complex B |
IntActi | P0C6T2, 3 interactors |
STRINGi | 9606.ENSP00000457935 |
Protein family/group databases
TCDBi | 9.B.142.3.17, the integral membrane glycosyltransferase family 39 (gt39) family |
Genetic variation databases
BioMutai | OST4 |
DMDMi | 182894143 |
Proteomic databases
jPOSTi | P0C6T2 |
MassIVEi | P0C6T2 |
PaxDbi | P0C6T2 |
PeptideAtlasi | P0C6T2 |
PRIDEi | P0C6T2 |
TopDownProteomicsi | P0C6T2 |
Protocols and materials databases
Antibodypediai | 64138, 15 antibodies from 9 providers |
DNASUi | 100128731 |
Genome annotation databases
Ensembli | ENST00000429985.1; ENSP00000455716.1; ENSG00000228474.6 ENST00000447619.5; ENSP00000454411.1; ENSG00000228474.6 ENST00000456793.2; ENSP00000457935.1; ENSG00000228474.6 |
GeneIDi | 100128731 |
KEGGi | hsa:100128731 |
MANE-Selecti | ENST00000456793.2; ENSP00000457935.1; NM_001134693.2; NP_001128165.1 |
UCSCi | uc002rig.4, human |
Organism-specific databases
CTDi | 100128731 |
DisGeNETi | 100128731 |
GeneCardsi | OST4 |
HGNCi | HGNC:32483, OST4 |
HPAi | ENSG00000228474, Low tissue specificity |
MIMi | 618932, gene |
neXtProti | NX_P0C6T2 |
OpenTargetsi | ENSG00000228474 |
PharmGKBi | PA165697090 |
VEuPathDBi | HostDB:ENSG00000228474 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502TACH, Eukaryota |
GeneTreei | ENSGT00390000016516 |
HOGENOMi | CLU_186352_2_0_1 |
InParanoidi | P0C6T2 |
OrthoDBi | 1584372at2759 |
PhylomeDBi | P0C6T2 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
PathwayCommonsi | P0C6T2 |
SignaLinki | P0C6T2 |
Miscellaneous databases
BioGRID-ORCSi | 100128731, 152 hits in 697 CRISPR screens |
ChiTaRSi | OST4, human |
GenomeRNAii | 100128731 |
Pharosi | P0C6T2, Tdark |
PROi | PR:P0C6T2 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000228474, Expressed in prostate gland and 216 other tissues |
Genevisiblei | P0C6T2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR018943, Oligosaccaryltransferase IPR036330, Ost4p_sf |
Pfami | View protein in Pfam PF10215, Ost4, 1 hit |
SUPFAMi | SSF103464, SSF103464, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OST4_HUMAN | |
Accessioni | P0C6T2Primary (citable) accession number: P0C6T2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | April 8, 2008 | |
Last modified: | May 25, 2022 | |
This is version 86 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families