UniProtKB - P0C6P5 (ARG28_RAT)
Rho guanine nucleotide exchange factor 28
Arhgef28
Functioni
Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.
2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 651 – 698 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 48 |
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- cell morphogenesis Source: GO_Central
- central nervous system neuron axonogenesis Source: RGD
- intracellular signal transduction Source: InterPro
- neurofilament cytoskeleton organization Source: RGD
- regulation of Rho protein signal transduction Source: GO_Central
Keywordsi
Molecular function | Guanine-nucleotide releasing factor, RNA-binding |
Biological process | Differentiation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-3928662, EPHB-mediated forward signaling R-RNO-8980692, RHOA GTPase cycle R-RNO-9013026, RHOB GTPase cycle R-RNO-9013106, RHOC GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Rho guanine nucleotide exchange factor 28Alternative name(s): 190 kDa guanine nucleotide exchange factor Short name: p190-RhoGEF Short name: p190RhoGEF Rho guanine nucleotide exchange factor |
Gene namesi | Name:Arhgef28 Synonyms:Rgnef |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1307061, Arhgef28 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity
Note: Colocalizes with the microtubule radial and cortical systems.By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000324120 | 1 – 1700 | Rho guanine nucleotide exchange factor 28Add BLAST | 1700 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 312 | PhosphoserineCombined sources | 1 | |
Modified residuei | 314 | PhosphoserineCombined sources | 1 | |
Modified residuei | 478 | PhosphoserineCombined sources | 1 | |
Modified residuei | 623 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1535 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1604 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0C6P5 |
PaxDbi | P0C6P5 |
PTM databases
iPTMneti | P0C6P5 |
PhosphoSitePlusi | P0C6P5 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000016544, Expressed in kidney and 22 other tissues |
Genevisiblei | P0C6P5, RN |
Interactioni
Subunit structurei
Homooligomer; forms cytoplasmic aggregates.
Forms a complex with MAPK8 and MAPK8IP1.
Interacts with RHOA.
Interacts with microtubules.
Interacts with YWHAE and YWHAH.
Interacts with PTK2/FAK1.
Interacts with NEFL.
Interacts with CTNND2; prevents interaction with RHOA (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000059196 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 848 – 1043 | DHPROSITE-ProRule annotationAdd BLAST | 196 | |
Domaini | 1085 – 1187 | PHPROSITE-ProRule annotationAdd BLAST | 103 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 288 – 335 | DisorderedSequence analysisAdd BLAST | 48 | |
Regioni | 483 – 525 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 709 – 761 | DisorderedSequence analysisAdd BLAST | 53 | |
Regioni | 774 – 799 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 1186 – 1207 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 1294 – 1303 | Interaction with PTK2/FAK1; required for regulation of axonal branching and synapse formationBy similarity | 10 | |
Regioni | 1369 – 1380 | Mediates cytoplasmic retention and interaction with YWHAHBy similarityAdd BLAST | 12 | |
Regioni | 1421 – 1700 | Interaction with microtubulesBy similarityAdd BLAST | 280 | |
Regioni | 1493 – 1524 | RNA-bindingAdd BLAST | 32 | |
Regioni | 1563 – 1576 | Mediates cytoplasmic retention and interaction with MAPK8IP1By similarityAdd BLAST | 14 | |
Regioni | 1574 – 1598 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 1611 – 1700 | DisorderedSequence analysisAdd BLAST | 90 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 1473 – 1522 | Sequence analysisAdd BLAST | 50 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 301 – 323 | Basic and acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 1192 – 1207 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 1611 – 1626 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 1642 – 1674 | Polar residuesSequence analysisAdd BLAST | 33 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 651 – 698 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 48 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG3519, Eukaryota KOG3520, Eukaryota |
GeneTreei | ENSGT00940000155831 |
HOGENOMi | CLU_002466_2_1_1 |
InParanoidi | P0C6P5 |
OMAi | EQECQSQ |
OrthoDBi | 69816at2759 |
PhylomeDBi | P0C6P5 |
TreeFami | TF334740 |
Family and domain databases
CDDi | cd00029, C1, 1 hit cd14680, PH_p190RhoGEF, 1 hit cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR037819, ARHGEF28_PH IPR035899, DBL_dom_sf IPR000219, DH-domain IPR002219, PE/DAG-bd IPR011993, PH-like_dom_sf IPR041020, PH_16 IPR001849, PH_domain |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF17838, PH_16, 1 hit PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MELSCSEVPL YGQRTVYAKF GKNVYLPEDA EFYFIYDGSH QRHVVIADRV
60 70 80 90 100
EDNVLQSIIP GHWLQETVMV SVCLCSEGYS PVTMGSGSVT YVDNMACRLA
110 120 130 140 150
RLLVTQADRL TPCSHQTLLT PFALTAEALP ALDEELVLAL TQLELPLGWT
160 170 180 190 200
VLGNSSLEVC LHRESLLHLA VRWALPKLFH FLLCLPGGVK ALELPNEEAT
210 220 230 240 250
TPLDLALHGG HSMLVEDISN FQGRRSPGFS RLRLNEDATL QFDRSSETLT
260 270 280 290 300
LTVNHTAEHL LEADIKLFRK YFWDRAFLVK ALDQEAKTER ATMPSGAAET
310 320 330 340 350
EEEVRNLESG RSPSEEEEDG QLVKSQADGP SEQEDQDRLV LDHSFDGLKK
360 370 380 390 400
SKHAPASLAA GQLSDVPNGG DEVYANCMVI DQVGDLDINY INIEGLSTHT
410 420 430 440 450
SPESMRSTLG PQACKHILPP DTSPCGHLLC EDSDRTLDAA ASQSCMSPPS
460 470 480 490 500
SHTSNLNLSF GLHGFVKEQS HLKKRSSSLD ALVADSEEEG RSEPPICYAV
510 520 530 540 550
GSQSSPRTGL PGGDELDSFD ANTEPDCNIS RTESLSLSST LHSKESLLSG
560 570 580 590 600
IRSRSYSCSS PKISSGKSRL VRDFTVCSSS EEQRSYSFQE PPGEKRIQEE
610 620 630 640 650
EWDDYIIPAK AESEKHKVSR TFSFLMNRMT SPRNKSKMKN KDTKDKEKLN
660 670 680 690 700
RHQFVPGTFS GVLQCSGCDK TLLGKESLQC ANCKANTHKG CKDTVPPCTK
710 720 730 740 750
KFQDKYNKNK PQTILGSSSV RDVPPPGLSL HPSPSMPIGL PAGRKETAGQ
760 770 780 790 800
AHPLSRSVPG TTLESFRRAV TSLESEGDSN SWRSRSHSDE LFQSMGSSPS
810 820 830 840 850
TESFMMEDVV DSSLWSELSS DAQEFEAESW SLVVDPSFCS RQEKDVIKRQ
860 870 880 890 900
DVIFELMQTE VHHIQTLLIM SEVFRKGMKE ELQLDHSTVD RIFPCLDELL
910 920 930 940 950
ETHKHFFFSM KERRQESCTG NDRNFVINQI GDILVQQFSE ENASKMKRIY
960 970 980 990 1000
GDFCSHHKEA MSLFKELQQN KKFQNFIKIR NSNLLARRRG IPECILLVTQ
1010 1020 1030 1040 1050
RITKYPVLVE RILQYTKERT EEHRDLCRAL GLIKDMIAAV DLKVSEYEKN
1060 1070 1080 1090 1100
QKWLEILNKI ENKTYTKLKN GHVFRKQALM SQERALLHDG LVYWKTATGR
1110 1120 1130 1140 1150
FKDILALLLT DVLLFLQEKD QKYIFAAVDQ KPSVISLQKL IAREVANEER
1160 1170 1180 1190 1200
GMFLISASSA GPEMYEIHTS SKEERNNWMR RIQQAVESCP EEEGGRTSES
1210 1220 1230 1240 1250
DEERRKADAR VAKIQQCQEI LSNQDQQICT YLEEKLHIYA ELGELSGFED
1260 1270 1280 1290 1300
VHLEPHLLIK PDPGEPPQAA SLLAAALREA ESLQVAVKAS KLADVSQPSE
1310 1320 1330 1340 1350
EGPGGAVLAD TLSAQDAPAS PTAFTEGTEG RGCWDVDPRL QGVVTDLAVS
1360 1370 1380 1390 1400
DAGEKVEYRS FSGSSQSEII QAIQNLTRLL YSLQAALTIQ DSHIEIHKLV
1410 1420 1430 1440 1450
LQQQESLAPS HSFRGGPLQD QEKSRYLEKH REELANIHKL QYQFQQEQRR
1460 1470 1480 1490 1500
WHRTCDQQQR EQEAQESWLQ ARERECQSQE ELLLRHRSEL DHQLQEYQQN
1510 1520 1530 1540 1550
LERLREGQRM VERERQRMRD QQGLLGHCKH SRQRSLPAVF SPGSKEVTEL
1560 1570 1580 1590 1600
NRAESLCHEK SFFLNDAFTH MSLNTSNKPN PSGAPWDAHP PGGSHLDLAR
1610 1620 1630 1640 1650
TSDSQIEVKM DVSQPSDVNS ELWTTGLGPQ RPARQESIKD SCKNVADLNS
1660 1670 1680 1690 1700
FQTESPDPQD SNQRGPQPQT LITEAKLTPP MAAGHGGDAG DGAEENIVYL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AABR03012977 Genomic DNA No translation available. AABR03013324 Genomic DNA No translation available. AABR03013680 Genomic DNA No translation available. AABR03015604 Genomic DNA No translation available. AABR03016111 Genomic DNA No translation available. AABR03017173 Genomic DNA No translation available. AABR03017199 Genomic DNA No translation available. AABR03018551 Genomic DNA No translation available. AABR03019008 Genomic DNA No translation available. AABR03021286 Genomic DNA No translation available. AABR03022481 Genomic DNA No translation available. |
RefSeqi | XP_008758898.1, XM_008760676.1 |
Genome annotation databases
Ensembli | ENSRNOT00000065143; ENSRNOP00000059196; ENSRNOG00000016544 |
GeneIDi | 361882 |
UCSCi | RGD:1307061, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AABR03012977 Genomic DNA No translation available. AABR03013324 Genomic DNA No translation available. AABR03013680 Genomic DNA No translation available. AABR03015604 Genomic DNA No translation available. AABR03016111 Genomic DNA No translation available. AABR03017173 Genomic DNA No translation available. AABR03017199 Genomic DNA No translation available. AABR03018551 Genomic DNA No translation available. AABR03019008 Genomic DNA No translation available. AABR03021286 Genomic DNA No translation available. AABR03022481 Genomic DNA No translation available. |
RefSeqi | XP_008758898.1, XM_008760676.1 |
3D structure databases
SMRi | P0C6P5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000059196 |
PTM databases
iPTMneti | P0C6P5 |
PhosphoSitePlusi | P0C6P5 |
Proteomic databases
jPOSTi | P0C6P5 |
PaxDbi | P0C6P5 |
Genome annotation databases
Ensembli | ENSRNOT00000065143; ENSRNOP00000059196; ENSRNOG00000016544 |
GeneIDi | 361882 |
UCSCi | RGD:1307061, rat |
Organism-specific databases
CTDi | 64283 |
RGDi | 1307061, Arhgef28 |
Phylogenomic databases
eggNOGi | KOG3519, Eukaryota KOG3520, Eukaryota |
GeneTreei | ENSGT00940000155831 |
HOGENOMi | CLU_002466_2_1_1 |
InParanoidi | P0C6P5 |
OMAi | EQECQSQ |
OrthoDBi | 69816at2759 |
PhylomeDBi | P0C6P5 |
TreeFami | TF334740 |
Enzyme and pathway databases
Reactomei | R-RNO-3928662, EPHB-mediated forward signaling R-RNO-8980692, RHOA GTPase cycle R-RNO-9013026, RHOB GTPase cycle R-RNO-9013106, RHOC GTPase cycle |
Miscellaneous databases
PROi | PR:P0C6P5 |
Gene expression databases
Bgeei | ENSRNOG00000016544, Expressed in kidney and 22 other tissues |
Genevisiblei | P0C6P5, RN |
Family and domain databases
CDDi | cd00029, C1, 1 hit cd14680, PH_p190RhoGEF, 1 hit cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR037819, ARHGEF28_PH IPR035899, DBL_dom_sf IPR000219, DH-domain IPR002219, PE/DAG-bd IPR011993, PH-like_dom_sf IPR041020, PH_16 IPR001849, PH_domain |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF17838, PH_16, 1 hit PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARG28_RAT | |
Accessioni | P0C6P5Primary (citable) accession number: P0C6P5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | March 18, 2008 | |
Last modified: | September 29, 2021 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |